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Protein

Prostaglandin reductase 3

Gene

ZADH2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest efficienty towards 15-keto-PGE2-alpha. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation.By similarity

Catalytic activityi

11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei185NADP; via amide nitrogen1 Publication1
Binding sitei205NADP1 Publication1
Binding sitei209NADP1 Publication1
Binding sitei224NADP1 Publication1
Binding sitei247NADP1 Publication1
Binding sitei269NADP; via carbonyl oxygen1 Publication1
Binding sitei275NADP1 Publication1
Binding sitei361NADP1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi303 – 305NADP1 Publication3

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciZFISH:ENSG00000180011-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin reductase 3 (EC:1.3.1.48By similarity)
Short name:
PRG-3
Alternative name(s):
Zinc-binding alcohol dehydrogenase domain-containing protein 2
Gene namesi
Name:ZADH2Imported
Synonyms:PTGR3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:28697. ZADH2.

Subcellular locationi

  • Peroxisome By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Organism-specific databases

DisGeNETi284273.
OpenTargetsiENSG00000180011.
PharmGKBiPA134951623.

Polymorphism and mutation databases

BioMutaiZADH2.
DMDMi74728888.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002234671 – 377Prostaglandin reductase 3Add BLAST377

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei35N6-acetyllysineBy similarity1
Modified residuei299PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8N4Q0.
MaxQBiQ8N4Q0.
PaxDbiQ8N4Q0.
PeptideAtlasiQ8N4Q0.
PRIDEiQ8N4Q0.

PTM databases

iPTMnetiQ8N4Q0.
PhosphoSitePlusiQ8N4Q0.

Expressioni

Gene expression databases

BgeeiENSG00000180011.
CleanExiHS_ZADH2.
ExpressionAtlasiQ8N4Q0. baseline and differential.
GenevisibleiQ8N4Q0. HS.

Organism-specific databases

HPAiHPA021468.
HPA053646.

Interactioni

Protein-protein interaction databases

BioGridi129813. 15 interactors.
IntActiQ8N4Q0. 1 interactor.
STRINGi9606.ENSP00000323678.

Structurei

Secondary structure

1377
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi33 – 38Combined sources6
Helixi45 – 48Combined sources4
Beta strandi49 – 56Combined sources8
Beta strandi64 – 73Combined sources10
Helixi78 – 82Combined sources5
Turni83 – 86Combined sources4
Beta strandi98 – 107Combined sources10
Helixi111 – 113Combined sources3
Beta strandi120 – 124Combined sources5
Beta strandi129 – 136Combined sources8
Helixi137 – 139Combined sources3
Beta strandi144 – 146Combined sources3
Helixi148 – 151Combined sources4
Turni152 – 155Combined sources4
Helixi156 – 167Combined sources12
Beta strandi175 – 178Combined sources4
Turni179 – 182Combined sources4
Helixi186 – 195Combined sources10
Beta strandi199 – 206Combined sources8
Helixi207 – 215Combined sources9
Beta strandi219 – 223Combined sources5
Turni224 – 226Combined sources3
Helixi229 – 236Combined sources8
Beta strandi241 – 246Combined sources6
Helixi251 – 259Combined sources9
Beta strandi260 – 268Combined sources9
Helixi272 – 274Combined sources3
Beta strandi277 – 279Combined sources3
Helixi289 – 296Combined sources8
Beta strandi299 – 302Combined sources4
Helixi305 – 307Combined sources3
Helixi309 – 311Combined sources3
Helixi312 – 324Combined sources13
Beta strandi338 – 340Combined sources3
Helixi348 – 357Combined sources10
Beta strandi362 – 368Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C0CX-ray1.45A/B33-371[»]
2WEKX-ray1.90A/B33-371[»]
2X1HX-ray1.75A/B33-371[»]
2X7HX-ray1.60A/B25-371[»]
ProteinModelPortaliQ8N4Q0.
SMRiQ8N4Q0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8N4Q0.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IQ9R. Eukaryota.
COG2130. LUCA.
GeneTreeiENSGT00390000009335.
HOGENOMiHOG000294682.
HOVERGENiHBG056525.
InParanoidiQ8N4Q0.
KOiK07119.
OMAiYMAPGSF.
OrthoDBiEOG091G0CTI.
PhylomeDBiQ8N4Q0.
TreeFamiTF328691.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
IPR002364. Quin_OxRdtase/zeta-crystal_CS.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS01162. QOR_ZETA_CRYSTAL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N4Q0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLRLVPTGAR AIVDMSYARH FLDFQGSAIP QAMQKLVVTR LSPNFREAVT
60 70 80 90 100
LSRDCPVPLP GDGDLLVRNR FVGVNASDIN YSAGRYDPSV KPPFDIGFEG
110 120 130 140 150
IGEVVALGLS ASARYTVGQA VAYMAPGSFA EYTVVPASIA TPVPSVKPEY
160 170 180 190 200
LTLLVSGTTA YISLKELGGL SEGKKVLVTA AAGGTGQFAM QLSKKAKCHV
210 220 230 240 250
IGTCSSDEKS AFLKSLGCDR PINYKTEPVG TVLKQEYPEG VDVVYESVGG
260 270 280 290 300
AMFDLAVDAL ATKGRLIVIG FISGYQTPTG LSPVKAGTLP AKLLKKSASV
310 320 330 340 350
QGFFLNHYLS KYQAAMSHLL EMCVSGDLVC EVDLGDLSPE GRFTGLESIF
360 370
RAVNYMYMGK NTGKIVVELP HSVNSKL
Length:377
Mass (Da):40,140
Last modified:October 1, 2002 - v1
Checksum:i9CDC927D815314D1
GO
Isoform 2 (identifier: Q8N4Q0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-123: Missing.

Note: No experimental confirmation available.
Show »
Length:254
Mass (Da):26,900
Checksum:i1335CE641CBEE387
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048202323C → F.Corresponds to variant rs17056661dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0557961 – 123Missing in isoform 2. 1 PublicationAdd BLAST123

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK292880 mRNA. Translation: BAF85569.1.
AK302801 mRNA. Translation: BAG64003.1.
AC025105 Genomic DNA. No translation available.
CH471117 Genomic DNA. Translation: EAW66569.1.
BC033780 mRNA. Translation: AAH33780.1.
BC078661 mRNA. Translation: AAH78661.1.
CCDSiCCDS12008.1. [Q8N4Q0-1]
CCDS77198.1. [Q8N4Q0-2]
RefSeqiNP_001293022.1. NM_001306093.1. [Q8N4Q0-2]
NP_787103.1. NM_175907.5. [Q8N4Q0-1]
UniGeneiHs.465433.
Hs.719383.

Genome annotation databases

EnsembliENST00000322342; ENSP00000323678; ENSG00000180011. [Q8N4Q0-1]
ENST00000537114; ENSP00000440111; ENSG00000180011. [Q8N4Q0-2]
GeneIDi284273.
KEGGihsa:284273.
UCSCiuc002llx.4. human. [Q8N4Q0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK292880 mRNA. Translation: BAF85569.1.
AK302801 mRNA. Translation: BAG64003.1.
AC025105 Genomic DNA. No translation available.
CH471117 Genomic DNA. Translation: EAW66569.1.
BC033780 mRNA. Translation: AAH33780.1.
BC078661 mRNA. Translation: AAH78661.1.
CCDSiCCDS12008.1. [Q8N4Q0-1]
CCDS77198.1. [Q8N4Q0-2]
RefSeqiNP_001293022.1. NM_001306093.1. [Q8N4Q0-2]
NP_787103.1. NM_175907.5. [Q8N4Q0-1]
UniGeneiHs.465433.
Hs.719383.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C0CX-ray1.45A/B33-371[»]
2WEKX-ray1.90A/B33-371[»]
2X1HX-ray1.75A/B33-371[»]
2X7HX-ray1.60A/B25-371[»]
ProteinModelPortaliQ8N4Q0.
SMRiQ8N4Q0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi129813. 15 interactors.
IntActiQ8N4Q0. 1 interactor.
STRINGi9606.ENSP00000323678.

PTM databases

iPTMnetiQ8N4Q0.
PhosphoSitePlusiQ8N4Q0.

Polymorphism and mutation databases

BioMutaiZADH2.
DMDMi74728888.

Proteomic databases

EPDiQ8N4Q0.
MaxQBiQ8N4Q0.
PaxDbiQ8N4Q0.
PeptideAtlasiQ8N4Q0.
PRIDEiQ8N4Q0.

Protocols and materials databases

DNASUi284273.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000322342; ENSP00000323678; ENSG00000180011. [Q8N4Q0-1]
ENST00000537114; ENSP00000440111; ENSG00000180011. [Q8N4Q0-2]
GeneIDi284273.
KEGGihsa:284273.
UCSCiuc002llx.4. human. [Q8N4Q0-1]

Organism-specific databases

CTDi284273.
DisGeNETi284273.
GeneCardsiZADH2.
HGNCiHGNC:28697. ZADH2.
HPAiHPA021468.
HPA053646.
neXtProtiNX_Q8N4Q0.
OpenTargetsiENSG00000180011.
PharmGKBiPA134951623.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IQ9R. Eukaryota.
COG2130. LUCA.
GeneTreeiENSGT00390000009335.
HOGENOMiHOG000294682.
HOVERGENiHBG056525.
InParanoidiQ8N4Q0.
KOiK07119.
OMAiYMAPGSF.
OrthoDBiEOG091G0CTI.
PhylomeDBiQ8N4Q0.
TreeFamiTF328691.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000180011-MONOMER.

Miscellaneous databases

ChiTaRSiZADH2. human.
EvolutionaryTraceiQ8N4Q0.
GenomeRNAii284273.
PROiQ8N4Q0.

Gene expression databases

BgeeiENSG00000180011.
CleanExiHS_ZADH2.
ExpressionAtlasiQ8N4Q0. baseline and differential.
GenevisibleiQ8N4Q0. HS.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
IPR002364. Quin_OxRdtase/zeta-crystal_CS.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS01162. QOR_ZETA_CRYSTAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTGR3_HUMAN
AccessioniPrimary (citable) accession number: Q8N4Q0
Secondary accession number(s): A8KA15, B4DZ91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.