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Protein

Kinesin-like protein KIF2B

Gene

KIF2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plus end-directed microtubule-dependent motor required for spindle assembly and chromosome movement. Has microtubule depolymerization activity (PubMed:17538014). Plays a role in chromosome congression (PubMed:23891108).2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi303 – 310ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
  • cell division Source: UniProtKB-KW
  • metaphase plate congression Source: UniProtKB
  • microtubule-based movement Source: GO_Central
  • microtubule depolymerization Source: UniProtKB
  • mitotic nuclear division Source: UniProtKB-KW
  • regulation of chromosome segregation Source: UniProtKB
  • retrograde vesicle-mediated transport, Golgi to ER Source: Reactome
  • sister chromatid cohesion Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-2132295. MHC class II antigen presentation.
R-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-HSA-68877. Mitotic Prometaphase.
R-HSA-983189. Kinesins.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIF2B
Gene namesi
Name:KIF2B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:29443. KIF2B.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule

Pathology & Biotechi

Organism-specific databases

DisGeNETi84643.
OpenTargetsiENSG00000141200.
PharmGKBiPA134928830.

Polymorphism and mutation databases

BioMutaiKIF2B.
DMDMi308153588.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002537151 – 673Kinesin-like protein KIF2BAdd BLAST673

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei125Phosphothreonine; by PLK11 Publication1
Modified residuei204Phosphoserine; by PLK11 Publication1

Post-translational modificationi

Phosphorylation at Thr-125 by PLK1 is required for activity in the correction of kinetochore-microtubules attachment errors, while phosphorylation at Ser-204 also by PLK1 is required for the kinetochore localization and activity in prometaphase.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8N4N8.
MaxQBiQ8N4N8.
PaxDbiQ8N4N8.
PeptideAtlasiQ8N4N8.
PRIDEiQ8N4N8.

PTM databases

iPTMnetiQ8N4N8.
PhosphoSitePlusiQ8N4N8.

Expressioni

Tissue specificityi

Highest level in lung. High level in ovary, moderate levels in heart, kidney, placenta, skeletal muscle and spleen (at protein level). Pancreas and spleen express a shorter isoform (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000141200.
CleanExiHS_KIF2B.
ExpressionAtlasiQ8N4N8. baseline and differential.
GenevisibleiQ8N4N8. HS.

Organism-specific databases

HPAiHPA023081.
HPA023103.
HPA024795.

Interactioni

Protein-protein interaction databases

BioGridi124167. 41 interactors.
IntActiQ8N4N8. 1 interactor.
STRINGi9606.ENSP00000268919.

Structurei

3D structure databases

ProteinModelPortaliQ8N4N8.
SMRiQ8N4N8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini213 – 543Kinesin motorPROSITE-ProRule annotationAdd BLAST331

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili151 – 176Sequence analysisAdd BLAST26
Coiled coili640 – 666Sequence analysisAdd BLAST27

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. MCAK/KIF2 subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0246. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00860000133670.
HOGENOMiHOG000231329.
HOVERGENiHBG003875.
InParanoidiQ8N4N8.
KOiK10393.
OMAiQTFCFDH.
OrthoDBiEOG091G06V3.
PhylomeDBiQ8N4N8.
TreeFamiTF105222.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 2 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8N4N8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASQFCLPES PCLSPLKPLK PHFGDIQEGI YVAIQRSDKR IHLAVVTEIN
60 70 80 90 100
RENYWVTVEW VEKAVKKGKK IDLETILLLN PALDSAEHPM PPPPLSPLAL
110 120 130 140 150
APSSAIRDQR TATKWVAMIP QKNQTASGDS LDVRVPSKPC LMKQKKSPCL
160 170 180 190 200
WEIQKLQEQR EKRRRLQQEI RARRALDVNT RNPNYEIMHM IEEYRRHLDS
210 220 230 240 250
SKISVLEPPQ EHRICVCVRK RPLNQRETTL KDLDIITVPS DNVVMVHESK
260 270 280 290 300
QKVDLTRYLQ NQTFCFDHAF DDKASNELVY QFTAQPLVES IFRKGMATCF
310 320 330 340 350
AYGQTGSGKT YTMGGDFSGT AQDCSKGIYA LVAQDVFLLL RNSTYEKLDL
360 370 380 390 400
KVYGTFFEIY GGKVYDLLNW KKKLQVLEDG NQQIQVVGLQ EKEVCCVEEV
410 420 430 440 450
LNLVEIGNSC RTSRQTPVNA HSSRSHAVFQ IILKSGRIMH GKFSLVDLAG
460 470 480 490 500
NERGADTTKA SRKRQLEGAE INKSLLALKE CILALGQNKP HTPFRASKLT
510 520 530 540 550
LVLRDSFIGQ NSSTCMIATI SPGMTSCENT LNTLRYANRV KKLNVDVRPY
560 570 580 590 600
HRGHYPIGHE APRMLKSHIG NSEMSLQRDE FIKIPYVQSE EQKEIEEVET
610 620 630 640 650
LPTLLGKDTT ISGKGSSQWL ENIQERAGGV HHDIDFCIAR SLSILEQKID
660 670
ALTEIQKKLK LLLADLHVKS KVE
Length:673
Mass (Da):76,254
Last modified:October 5, 2010 - v3
Checksum:iA0986D95FCF116F1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32V → A in AAK20830 (Ref. 1) Curated1
Sequence conflicti49I → T in BAB71406 (PubMed:14702039).Curated1
Sequence conflicti93P → L in AAK20830 (Ref. 1) Curated1
Sequence conflicti306G → R in AAK20830 (Ref. 1) Curated1
Sequence conflicti313M → V in AAK20830 (Ref. 1) Curated1
Sequence conflicti320T → K in BAB71406 (PubMed:14702039).Curated1
Sequence conflicti500T → A in AAK20830 (Ref. 1) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_028717112A → V.2 PublicationsCorresponds to variant rs3803824dbSNPEnsembl.1
Natural variantiVAR_028718128G → R.Corresponds to variant rs9912492dbSNPEnsembl.1
Natural variantiVAR_061279148P → S.Corresponds to variant rs59657238dbSNPEnsembl.1
Natural variantiVAR_028719417P → S.3 PublicationsCorresponds to variant rs4561518dbSNPEnsembl.1
Natural variantiVAR_028720437R → G.3 PublicationsCorresponds to variant rs4561519dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF333335 mRNA. Translation: AAK20830.2.
AK057272 mRNA. Translation: BAB71406.1.
AC019315 Genomic DNA. No translation available.
BC033802 mRNA. Translation: AAH33802.1.
CCDSiCCDS32685.1.
RefSeqiNP_115948.4. NM_032559.4.
UniGeneiHs.226805.
Hs.744513.

Genome annotation databases

EnsembliENST00000268919; ENSP00000268919; ENSG00000141200.
GeneIDi84643.
KEGGihsa:84643.
UCSCiuc002iua.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF333335 mRNA. Translation: AAK20830.2.
AK057272 mRNA. Translation: BAB71406.1.
AC019315 Genomic DNA. No translation available.
BC033802 mRNA. Translation: AAH33802.1.
CCDSiCCDS32685.1.
RefSeqiNP_115948.4. NM_032559.4.
UniGeneiHs.226805.
Hs.744513.

3D structure databases

ProteinModelPortaliQ8N4N8.
SMRiQ8N4N8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124167. 41 interactors.
IntActiQ8N4N8. 1 interactor.
STRINGi9606.ENSP00000268919.

PTM databases

iPTMnetiQ8N4N8.
PhosphoSitePlusiQ8N4N8.

Polymorphism and mutation databases

BioMutaiKIF2B.
DMDMi308153588.

Proteomic databases

EPDiQ8N4N8.
MaxQBiQ8N4N8.
PaxDbiQ8N4N8.
PeptideAtlasiQ8N4N8.
PRIDEiQ8N4N8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000268919; ENSP00000268919; ENSG00000141200.
GeneIDi84643.
KEGGihsa:84643.
UCSCiuc002iua.3. human.

Organism-specific databases

CTDi84643.
DisGeNETi84643.
GeneCardsiKIF2B.
H-InvDBHIX0013999.
HGNCiHGNC:29443. KIF2B.
HPAiHPA023081.
HPA023103.
HPA024795.
MIMi615142. gene.
neXtProtiNX_Q8N4N8.
OpenTargetsiENSG00000141200.
PharmGKBiPA134928830.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0246. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00860000133670.
HOGENOMiHOG000231329.
HOVERGENiHBG003875.
InParanoidiQ8N4N8.
KOiK10393.
OMAiQTFCFDH.
OrthoDBiEOG091G06V3.
PhylomeDBiQ8N4N8.
TreeFamiTF105222.

Enzyme and pathway databases

ReactomeiR-HSA-2132295. MHC class II antigen presentation.
R-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-HSA-68877. Mitotic Prometaphase.
R-HSA-983189. Kinesins.

Miscellaneous databases

GenomeRNAii84643.
PROiQ8N4N8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000141200.
CleanExiHS_KIF2B.
ExpressionAtlasiQ8N4N8. baseline and differential.
GenevisibleiQ8N4N8. HS.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 2 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKIF2B_HUMAN
AccessioniPrimary (citable) accession number: Q8N4N8
Secondary accession number(s): Q96MA2, Q9BXG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 5, 2010
Last modified: November 30, 2016
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Osteosarcoma cells (U2OS) lacking KIF2B show disorganised often monopolar mitotic spindles, severely reduced velocity of chromosome movement and blocked cytokinesis. Bipolar mitotic spindles can be restored by simultaneous depletion of KIF2B, KIFC1 and NUMA1.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.