Q8N4C8 (MINK1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Misshapen-like kinase 1
EC=2.7.11.1

Alternative name(s):
GCK family kinase MiNK
MAPK/ERK kinase kinase kinase 6
Short name=MEK kinase kinase 6
Short name=MEKKK 6
Misshapen/NIK-related kinase
Mitogen-activated protein kinase kinase kinase kinase 6

Gene names

Name:	MINK1
Synonyms:	B55, MAP4K6, MINK, YSK2, ZC3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1332 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking. Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons. Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates: TANC1 upon stimulation by RAP2A, MBP and SMAD1. Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1. Ref.2 Ref.7 Ref.9 Ref.18 Isoform 4 can activate the JNK pathway. Involved in the regulation of actin cytoskeleton reorganization, cell-matrix adhesion, cell-cell adhesion and cell migration. Ref.2 Ref.7 Ref.9 Ref.18
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB Q9JM52
Cofactor	Magnesium By similarity. UniProtKB Q9JM52
Subunit structure	Interacts with TANC1 By similarity. Interacts with RAP2A. Isoform 4 interacts with NCK1. Ref.2 Ref.9
Subcellular location	Cytoplasm. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density By similarity. Cell projection › axon By similarity. Cell projection › dendrite By similarity Ref.2 Ref.9. Isoform 4: Golgi apparatus Ref.2 Ref.9.
Tissue specificity	Expressed in the brain, isoform 2 is more abundant than isoform 1. Isoform 3 is ubiquitously expressed. Isoform 1 is most abundant in the skeletal muscle. Isoform 4 is ubiquitously expressed with relative high levels in brain, skeletal muscle, pancreas and testis. Ref.1 Ref.2
Induction	Activated after Ras induction via a mechanism involving reactive oxygen species. Ref.7
Post-translational modification	Autophosphorylated. Ref.2
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CNH domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Cell projection Cytoplasm Golgi apparatus Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	JNK cascade Traceable author statement Ref.1. Source: ProtInc actin cytoskeleton reorganization Inferred from mutant phenotype Ref.2. Source: UniProtKB dendrite morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB negative thymic T cell selection Inferred from electronic annotation. Source: Compara positive regulation of JNK cascade Inferred from mutant phenotype Ref.2. Source: UniProtKB protein autophosphorylation Inferred from direct assay Ref.2. Source: UniProtKB regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Inferred from sequence or structural similarity. Source: UniProtKB regulation of cell migration Inferred from mutant phenotype Ref.2. Source: UniProtKB regulation of cell-cell adhesion Inferred from mutant phenotype Ref.2. Source: UniProtKB regulation of cell-matrix adhesion Inferred from mutant phenotype Ref.2. Source: UniProtKB synaptic transmission Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	Golgi apparatus Inferred from direct assay Ref.2. Source: UniProtKB axon Inferred from electronic annotation. Source: UniProtKB-SubCell cell junction Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW postsynaptic density Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from sequence or structural similarity. Source: UniProtKB protein serine/threonine kinase activity Inferred from direct assay Ref.9Ref.18. Source: UniProtKB small GTPase regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 3 Ref.1 (identifier: Q8N4C8-1)

Also known as: MINK-alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 Ref.1 (identifier: Q8N4C8-2)

Also known as: MiNK-1; MINK-delta;

The sequence of this isoform differs from the canonical sequence as follows:
     696-732: Missing.

Isoform 2 Ref.1 (identifier: Q8N4C8-3)

Also known as: MiNK-2; MINK-gamma;

The sequence of this isoform differs from the canonical sequence as follows:
     696-732: Missing.
     800-800: A → ASYKRAIGE

Isoform 4 (identifier: Q8N4C8-4)

Also known as: MINK-beta;

The sequence of this isoform differs from the canonical sequence as follows:
     581-600: Missing.

Isoform 5 (identifier: Q8N4C8-5)

Also known as: MINK-eta;

The sequence of this isoform differs from the canonical sequence as follows:
     581-600: Missing.
     696-732: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1332	1332	Misshapen-like kinase 1		PRO_0000086329
Regions
	Domain	25 – 289	265	Protein kinase
	Domain	1019 – 1306	288	CNH
	Nucleotide binding	31 – 39	9	ATP By similarity UniProtKB O00506
	Region	866 – 1332	467	Mediates interaction with RAP2A
	Compositional bias	359 – 495	137	Gln-rich
	Compositional bias	542 – 729	188	Pro-rich
Sites
	Active site	153	1	Proton acceptor By similarity UniProtKB O00506
	Binding site	54	1	ATP By similarity UniProtKB O00506
Amino acid modifications
	Modified residue	641	1	Phosphoserine Ref.12
	Modified residue	763	1	Phosphoserine Ref.8 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.16 Ref.19
	Modified residue	777	1	Phosphoserine Ref.13
	Modified residue	778	1	Phosphoserine Ref.13 Ref.15
	Modified residue	782	1	Phosphoserine Ref.12 Ref.13
	Modified residue	916	1	Phosphoserine By similarity
	Modified residue	993	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	581 – 600	20	Missing in isoform 4 and isoform 5.		VSP_041871
	Alternative sequence	696 – 732	37	Missing in isoform 1, isoform 2 and isoform 5. Ref.1		VSP_007059
	Alternative sequence	800	1	A → ASYKRAIGE in isoform 2. Ref.1		VSP_007060
	Natural variant	514	1	A → T. Ref.20 Corresponds to variant rs56131206 [ dbSNP | Ensembl ].		VAR_040799
	Natural variant	771	1	V → A. Ref.2 Ref.6 Corresponds to variant rs11556634 [ dbSNP | Ensembl ].		VAR_046058
	Natural variant	775	1	P → L. Ref.2 Ref.6 Ref.12 Ref.13 Ref.14 Ref.15 Corresponds to variant rs11556635 [ dbSNP | Ensembl ].		VAR_046059
	Natural variant	863	1	V → I. Ref.20 Corresponds to variant rs2302319 [ dbSNP | Ensembl ].		VAR_046060
	Natural variant	1010	1	E → V in a gastric adenocarcinoma sample; somatic mutation. Ref.20		VAR_046061
	Natural variant	1200	1	I → V. Ref.20		VAR_040800

Sequences

	Sequence		Length	Mass (Da)
	Isoform 3 (MINK-alpha) [UniParc]. Last modified May 18, 2010. Version 2. Checksum: 3E3B182CDDB3659C	FASTA	1,332	149,822
	10 20 30 40 50 60 MGDPAPARSL DDIDLSALRD PAGIFELVEV VGNGTYGQVY KGRHVKTGQL AAIKVMDVTE 70 80 90 100 110 120 DEEEEIKQEI NMLKKYSHHR NIATYYGAFI KKSPPGNDDQ LWLVMEFCGA GSVTDLVKNT 130 140 150 160 170 180 KGNALKEDCI AYICREILRG LAHLHAHKVI HRDIKGQNVL LTENAEVKLV DFGVSAQLDR 190 200 210 220 230 240 TVGRRNTFIG TPYWMAPEVI ACDENPDATY DYRSDIWSLG ITAIEMAEGA PPLCDMHPMR 250 260 270 280 290 300 ALFLIPRNPP PRLKSKKWSK KFIDFIDTCL IKTYLSRPPT EQLLKFPFIR DQPTERQVRI 310 320 330 340 350 360 QLKDHIDRSR KKRGEKEETE YEYSGSEEED DSHGEEGEPS SIMNVPGEST LRREFLRLQQ 370 380 390 400 410 420 ENKSNSEALK QQQQLQQQQQ RDPEAHIKHL LHQRQRRIEE QKEERRRVEE QQRREREQRK 430 440 450 460 470 480 LQEKEQQRRL EDMQALRREE ERRQAEREQE YKRKQLEEQR QSERLQRQLQ QEHAYLKSLQ 490 500 510 520 530 540 QQQQQQQLQK QQQQQLLPGD RKPLYHYGRG MNPADKPAWA REVEERTRMN KQQNSPLAKS 550 560 570 580 590 600 KPGSTGPEPP IPQASPGPPG PLSQTPPMQR PVEPQEGPHK SLVAHRVPLK PYAAPVPRSQ 610 620 630 640 650 660 SLQDQPTRNL AAFPASHDPD PAIPAPTATP SARGAVIRQN SDPTSEGPGP SPNPPAWVRP 670 680 690 700 710 720 DNEAPPKVPQ RTSSIATALN TSGAGGSRPA QAVRARPRSN SAWQIYLQRR AERGTPKPPG 730 740 750 760 770 780 PPAQPPGPPN ASSNPDLRRS DPGWERSDSV LPASHGHLPQ AGSLERNRVG VSSKPDSSPV 790 800 810 820 830 840 LSPGNKAKPD DHRSRPGRPA DFVLLKERTL DEAPRPPKKA MDYSSSSEEV ESSEDDEEEG 850 860 870 880 890 900 EGGPAEGSRD TPGGRSDGDT DSVSTMVVHD VEEITGTQPP YGGGTMVVQR TPEEERNLLH 910 920 930 940 950 960 ADSNGYTNLP DVVQPSHSPT ENSKGQSPPS KDGSGDYQSR GLVKAPGKSS FTMFVDLGIY 970 980 990 1000 1010 1020 QPGGSGDSIP ITALVGGEGT RLDQLQYDVR KGSVVNVNPT NTRAHSETPE IRKYKKRFNS 1030 1040 1050 1060 1070 1080 EILCAALWGV NLLVGTENGL MLLDRSGQGK VYGLIGRRRF QQMDVLEGLN LLITISGKRN 1090 1100 1110 1120 1130 1140 KLRVYYLSWL RNKILHNDPE VEKKQGWTTV GDMEGCGHYR VVKYERIKFL VIALKSSVEV 1150 1160 1170 1180 1190 1200 YAWAPKPYHK FMAFKSFADL PHRPLLVDLT VEEGQRLKVI YGSSAGFHAV DVDSGNSYDI 1210 1220 1230 1240 1250 1260 YIPVHIQSQI TPHAIIFLPN TDGMEMLLCY EDEGVYVNTY GRIIKDVVLQ WGEMPTSVAY 1270 1280 1290 1300 1310 1320 ICSNQIMGWG EKAIEIRSVE TGHLDGVFMH KRAQRLKFLC ERNDKVFFAS VRSGGSSQVY 1330 FMTLNRNCIM NW
	Isoform 1 (MiNK-1) (MINK-delta) [UniParc]. Checksum: 029AD8C2806C90EE	FASTA	1,295	145,806
	10 20 30 40 50 60 MGDPAPARSL DDIDLSALRD PAGIFELVEV VGNGTYGQVY KGRHVKTGQL AAIKVMDVTE 70 80 90 100 110 120 DEEEEIKQEI NMLKKYSHHR NIATYYGAFI KKSPPGNDDQ LWLVMEFCGA GSVTDLVKNT 130 140 150 160 170 180 KGNALKEDCI AYICREILRG LAHLHAHKVI HRDIKGQNVL LTENAEVKLV DFGVSAQLDR 190 200 210 220 230 240 TVGRRNTFIG TPYWMAPEVI ACDENPDATY DYRSDIWSLG ITAIEMAEGA PPLCDMHPMR 250 260 270 280 290 300 ALFLIPRNPP PRLKSKKWSK KFIDFIDTCL IKTYLSRPPT EQLLKFPFIR DQPTERQVRI 310 320 330 340 350 360 QLKDHIDRSR KKRGEKEETE YEYSGSEEED DSHGEEGEPS SIMNVPGEST LRREFLRLQQ 370 380 390 400 410 420 ENKSNSEALK QQQQLQQQQQ RDPEAHIKHL LHQRQRRIEE QKEERRRVEE QQRREREQRK 430 440 450 460 470 480 LQEKEQQRRL EDMQALRREE ERRQAEREQE YKRKQLEEQR QSERLQRQLQ QEHAYLKSLQ 490 500 510 520 530 540 QQQQQQQLQK QQQQQLLPGD RKPLYHYGRG MNPADKPAWA REVEERTRMN KQQNSPLAKS 550 560 570 580 590 600 KPGSTGPEPP IPQASPGPPG PLSQTPPMQR PVEPQEGPHK SLVAHRVPLK PYAAPVPRSQ 610 620 630 640 650 660 SLQDQPTRNL AAFPASHDPD PAIPAPTATP SARGAVIRQN SDPTSEGPGP SPNPPAWVRP 670 680 690 700 710 720 DNEAPPKVPQ RTSSIATALN TSGAGGSRPA QAVRASNPDL RRSDPGWERS DSVLPASHGH 730 740 750 760 770 780 LPQAGSLERN RVGVSSKPDS SPVLSPGNKA KPDDHRSRPG RPADFVLLKE RTLDEAPRPP 790 800 810 820 830 840 KKAMDYSSSS EEVESSEDDE EEGEGGPAEG SRDTPGGRSD GDTDSVSTMV VHDVEEITGT 850 860 870 880 890 900 QPPYGGGTMV VQRTPEEERN LLHADSNGYT NLPDVVQPSH SPTENSKGQS PPSKDGSGDY 910 920 930 940 950 960 QSRGLVKAPG KSSFTMFVDL GIYQPGGSGD SIPITALVGG EGTRLDQLQY DVRKGSVVNV 970 980 990 1000 1010 1020 NPTNTRAHSE TPEIRKYKKR FNSEILCAAL WGVNLLVGTE NGLMLLDRSG QGKVYGLIGR 1030 1040 1050 1060 1070 1080 RRFQQMDVLE GLNLLITISG KRNKLRVYYL SWLRNKILHN DPEVEKKQGW TTVGDMEGCG 1090 1100 1110 1120 1130 1140 HYRVVKYERI KFLVIALKSS VEVYAWAPKP YHKFMAFKSF ADLPHRPLLV DLTVEEGQRL 1150 1160 1170 1180 1190 1200 KVIYGSSAGF HAVDVDSGNS YDIYIPVHIQ SQITPHAIIF LPNTDGMEML LCYEDEGVYV 1210 1220 1230 1240 1250 1260 NTYGRIIKDV VLQWGEMPTS VAYICSNQIM GWGEKAIEIR SVETGHLDGV FMHKRAQRLK 1270 1280 1290 FLCERNDKVF FASVRSGGSS QVYFMTLNRN CIMNW
	Isoform 2 (MiNK-2) (MINK-gamma) [UniParc]. Checksum: FD65470BFC6981B2	FASTA	1,303	146,711
	10 20 30 40 50 60 MGDPAPARSL DDIDLSALRD PAGIFELVEV VGNGTYGQVY KGRHVKTGQL AAIKVMDVTE 70 80 90 100 110 120 DEEEEIKQEI NMLKKYSHHR NIATYYGAFI KKSPPGNDDQ LWLVMEFCGA GSVTDLVKNT 130 140 150 160 170 180 KGNALKEDCI AYICREILRG LAHLHAHKVI HRDIKGQNVL LTENAEVKLV DFGVSAQLDR 190 200 210 220 230 240 TVGRRNTFIG TPYWMAPEVI ACDENPDATY DYRSDIWSLG ITAIEMAEGA PPLCDMHPMR 250 260 270 280 290 300 ALFLIPRNPP PRLKSKKWSK KFIDFIDTCL IKTYLSRPPT EQLLKFPFIR DQPTERQVRI 310 320 330 340 350 360 QLKDHIDRSR KKRGEKEETE YEYSGSEEED DSHGEEGEPS SIMNVPGEST LRREFLRLQQ 370 380 390 400 410 420 ENKSNSEALK QQQQLQQQQQ RDPEAHIKHL LHQRQRRIEE QKEERRRVEE QQRREREQRK 430 440 450 460 470 480 LQEKEQQRRL EDMQALRREE ERRQAEREQE YKRKQLEEQR QSERLQRQLQ QEHAYLKSLQ 490 500 510 520 530 540 QQQQQQQLQK QQQQQLLPGD RKPLYHYGRG MNPADKPAWA REVEERTRMN KQQNSPLAKS 550 560 570 580 590 600 KPGSTGPEPP IPQASPGPPG PLSQTPPMQR PVEPQEGPHK SLVAHRVPLK PYAAPVPRSQ 610 620 630 640 650 660 SLQDQPTRNL AAFPASHDPD PAIPAPTATP SARGAVIRQN SDPTSEGPGP SPNPPAWVRP 670 680 690 700 710 720 DNEAPPKVPQ RTSSIATALN TSGAGGSRPA QAVRASNPDL RRSDPGWERS DSVLPASHGH 730 740 750 760 770 780 LPQAGSLERN RVGVSSKPDS SPVLSPGNKA KPDDHRSRPG RPASYKRAIG EDFVLLKERT 790 800 810 820 830 840 LDEAPRPPKK AMDYSSSSEE VESSEDDEEE GEGGPAEGSR DTPGGRSDGD TDSVSTMVVH 850 860 870 880 890 900 DVEEITGTQP PYGGGTMVVQ RTPEEERNLL HADSNGYTNL PDVVQPSHSP TENSKGQSPP 910 920 930 940 950 960 SKDGSGDYQS RGLVKAPGKS SFTMFVDLGI YQPGGSGDSI PITALVGGEG TRLDQLQYDV 970 980 990 1000 1010 1020 RKGSVVNVNP TNTRAHSETP EIRKYKKRFN SEILCAALWG VNLLVGTENG LMLLDRSGQG 1030 1040 1050 1060 1070 1080 KVYGLIGRRR FQQMDVLEGL NLLITISGKR NKLRVYYLSW LRNKILHNDP EVEKKQGWTT 1090 1100 1110 1120 1130 1140 VGDMEGCGHY RVVKYERIKF LVIALKSSVE VYAWAPKPYH KFMAFKSFAD LPHRPLLVDL 1150 1160 1170 1180 1190 1200 TVEEGQRLKV IYGSSAGFHA VDVDSGNSYD IYIPVHIQSQ ITPHAIIFLP NTDGMEMLLC 1210 1220 1230 1240 1250 1260 YEDEGVYVNT YGRIIKDVVL QWGEMPTSVA YICSNQIMGW GEKAIEIRSV ETGHLDGVFM 1270 1280 1290 1300 HKRAQRLKFL CERNDKVFFA SVRSGGSSQV YFMTLNRNCI MNW
	Isoform 4 (MINK-beta) [UniParc]. Checksum: B8D157A582DA5D18	FASTA	1,312	147,653
	10 20 30 40 50 60 MGDPAPARSL DDIDLSALRD PAGIFELVEV VGNGTYGQVY KGRHVKTGQL AAIKVMDVTE 70 80 90 100 110 120 DEEEEIKQEI NMLKKYSHHR NIATYYGAFI KKSPPGNDDQ LWLVMEFCGA GSVTDLVKNT 130 140 150 160 170 180 KGNALKEDCI AYICREILRG LAHLHAHKVI HRDIKGQNVL LTENAEVKLV DFGVSAQLDR 190 200 210 220 230 240 TVGRRNTFIG TPYWMAPEVI ACDENPDATY DYRSDIWSLG ITAIEMAEGA PPLCDMHPMR 250 260 270 280 290 300 ALFLIPRNPP PRLKSKKWSK KFIDFIDTCL IKTYLSRPPT EQLLKFPFIR DQPTERQVRI 310 320 330 340 350 360 QLKDHIDRSR KKRGEKEETE YEYSGSEEED DSHGEEGEPS SIMNVPGEST LRREFLRLQQ 370 380 390 400 410 420 ENKSNSEALK QQQQLQQQQQ RDPEAHIKHL LHQRQRRIEE QKEERRRVEE QQRREREQRK 430 440 450 460 470 480 LQEKEQQRRL EDMQALRREE ERRQAEREQE YKRKQLEEQR QSERLQRQLQ QEHAYLKSLQ 490 500 510 520 530 540 QQQQQQQLQK QQQQQLLPGD RKPLYHYGRG MNPADKPAWA REVEERTRMN KQQNSPLAKS 550 560 570 580 590 600 KPGSTGPEPP IPQASPGPPG PLSQTPPMQR PVEPQEGPHK SLQDQPTRNL AAFPASHDPD 610 620 630 640 650 660 PAIPAPTATP SARGAVIRQN SDPTSEGPGP SPNPPAWVRP DNEAPPKVPQ RTSSIATALN 670 680 690 700 710 720 TSGAGGSRPA QAVRARPRSN SAWQIYLQRR AERGTPKPPG PPAQPPGPPN ASSNPDLRRS 730 740 750 760 770 780 DPGWERSDSV LPASHGHLPQ AGSLERNRVG VSSKPDSSPV LSPGNKAKPD DHRSRPGRPA 790 800 810 820 830 840 DFVLLKERTL DEAPRPPKKA MDYSSSSEEV ESSEDDEEEG EGGPAEGSRD TPGGRSDGDT 850 860 870 880 890 900 DSVSTMVVHD VEEITGTQPP YGGGTMVVQR TPEEERNLLH ADSNGYTNLP DVVQPSHSPT 910 920 930 940 950 960 ENSKGQSPPS KDGSGDYQSR GLVKAPGKSS FTMFVDLGIY QPGGSGDSIP ITALVGGEGT 970 980 990 1000 1010 1020 RLDQLQYDVR KGSVVNVNPT NTRAHSETPE IRKYKKRFNS EILCAALWGV NLLVGTENGL 1030 1040 1050 1060 1070 1080 MLLDRSGQGK VYGLIGRRRF QQMDVLEGLN LLITISGKRN KLRVYYLSWL RNKILHNDPE 1090 1100 1110 1120 1130 1140 VEKKQGWTTV GDMEGCGHYR VVKYERIKFL VIALKSSVEV YAWAPKPYHK FMAFKSFADL 1150 1160 1170 1180 1190 1200 PHRPLLVDLT VEEGQRLKVI YGSSAGFHAV DVDSGNSYDI YIPVHIQSQI TPHAIIFLPN 1210 1220 1230 1240 1250 1260 TDGMEMLLCY EDEGVYVNTY GRIIKDVVLQ WGEMPTSVAY ICSNQIMGWG EKAIEIRSVE 1270 1280 1290 1300 1310 TGHLDGVFMH KRAQRLKFLC ERNDKVFFAS VRSGGSSQVY FMTLNRNCIM NW
	Isoform 5 (MINK-eta) [UniParc]. Checksum: 4E992F63A486A95A	FASTA	1,275	143,637
	10 20 30 40 50 60 MGDPAPARSL DDIDLSALRD PAGIFELVEV VGNGTYGQVY KGRHVKTGQL AAIKVMDVTE 70 80 90 100 110 120 DEEEEIKQEI NMLKKYSHHR NIATYYGAFI KKSPPGNDDQ LWLVMEFCGA GSVTDLVKNT 130 140 150 160 170 180 KGNALKEDCI AYICREILRG LAHLHAHKVI HRDIKGQNVL LTENAEVKLV DFGVSAQLDR 190 200 210 220 230 240 TVGRRNTFIG TPYWMAPEVI ACDENPDATY DYRSDIWSLG ITAIEMAEGA PPLCDMHPMR 250 260 270 280 290 300 ALFLIPRNPP PRLKSKKWSK KFIDFIDTCL IKTYLSRPPT EQLLKFPFIR DQPTERQVRI 310 320 330 340 350 360 QLKDHIDRSR KKRGEKEETE YEYSGSEEED DSHGEEGEPS SIMNVPGEST LRREFLRLQQ 370 380 390 400 410 420 ENKSNSEALK QQQQLQQQQQ RDPEAHIKHL LHQRQRRIEE QKEERRRVEE QQRREREQRK 430 440 450 460 470 480 LQEKEQQRRL EDMQALRREE ERRQAEREQE YKRKQLEEQR QSERLQRQLQ QEHAYLKSLQ 490 500 510 520 530 540 QQQQQQQLQK QQQQQLLPGD RKPLYHYGRG MNPADKPAWA REVEERTRMN KQQNSPLAKS 550 560 570 580 590 600 KPGSTGPEPP IPQASPGPPG PLSQTPPMQR PVEPQEGPHK SLQDQPTRNL AAFPASHDPD 610 620 630 640 650 660 PAIPAPTATP SARGAVIRQN SDPTSEGPGP SPNPPAWVRP DNEAPPKVPQ RTSSIATALN 670 680 690 700 710 720 TSGAGGSRPA QAVRASNPDL RRSDPGWERS DSVLPASHGH LPQAGSLERN RVGVSSKPDS 730 740 750 760 770 780 SPVLSPGNKA KPDDHRSRPG RPADFVLLKE RTLDEAPRPP KKAMDYSSSS EEVESSEDDE 790 800 810 820 830 840 EEGEGGPAEG SRDTPGGRSD GDTDSVSTMV VHDVEEITGT QPPYGGGTMV VQRTPEEERN 850 860 870 880 890 900 LLHADSNGYT NLPDVVQPSH SPTENSKGQS PPSKDGSGDY QSRGLVKAPG KSSFTMFVDL 910 920 930 940 950 960 GIYQPGGSGD SIPITALVGG EGTRLDQLQY DVRKGSVVNV NPTNTRAHSE TPEIRKYKKR 970 980 990 1000 1010 1020 FNSEILCAAL WGVNLLVGTE NGLMLLDRSG QGKVYGLIGR RRFQQMDVLE GLNLLITISG 1030 1040 1050 1060 1070 1080 KRNKLRVYYL SWLRNKILHN DPEVEKKQGW TTVGDMEGCG HYRVVKYERI KFLVIALKSS 1090 1100 1110 1120 1130 1140 VEVYAWAPKP YHKFMAFKSF ADLPHRPLLV DLTVEEGQRL KVIYGSSAGF HAVDVDSGNS 1150 1160 1170 1180 1190 1200 YDIYIPVHIQ SQITPHAIIF LPNTDGMEML LCYEDEGVYV NTYGRIIKDV VLQWGEMPTS 1210 1220 1230 1240 1250 1260 VAYICSNQIM GWGEKAIEIR SVETGHLDGV FMHKRAQRLK FLCERNDKVF FASVRSGGSS 1270 QVYFMTLNRN CIMNW

References

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[1]	"Molecular cloning of MINK, a novel member of mammalian GCK family kinases, which is up-regulated during postnatal mouse cerebral development." Dan I., Watanabe N.M., Kobayashi T., Yamashita-Suzuki K., Fukagaya Y., Kajikawa E., Kimura W.K., Nakashima T.M., Matsumoto K., Ninomiya-Tsuji J., Kusumi A. FEBS Lett. 469:19-23(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Brain.
[2]	"Identification and functional characterization of a novel human misshapen/Nck interacting kinase-related kinase, hMINK beta." Hu Y., Leo C., Yu S., Huang B.C., Wang H., Shen M., Luo Y., Daniel-Issakani S., Payan D.G., Xu X. J. Biol. Chem. 279:54387-54397(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH NCK1, TISSUE SPECIFICITY, ALTERNATIVE SPLICING, AUTOPHOSPHORYLATION, VARIANTS ALA-771 AND LEU-775.
[3]	Erratum Hu Y., Leo C., Yu S., Huang B.C., Wang H., Shen M., Luo Y., Daniel-Issakani S., Payan D.G., Xu X. J. Biol. Chem. 280:5128-5128(2005)
[4]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANTS ALA-771 AND LEU-775. Tissue: Lymph.
[7]	"Involvement of MINK, a Ste20 family kinase, in Ras oncogene-induced growth arrest in human ovarian surface epithelial cells." Nicke B., Bastien J., Khanna S.J., Warne P.H., Cowling V., Cook S.J., Peters G., Delpuech O., Schulze A., Berns K., Mullenders J., Beijersbergen R.L., Bernards R., Ganesan T.S., Downward J., Hancock D.C. Mol. Cell 20:673-685(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
[8]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-763, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"MINK is a Rap2 effector for phosphorylation of the postsynaptic scaffold protein TANC1." Nonaka H., Takei K., Umikawa M., Oshiro M., Kuninaka K., Bayarjargal M., Asato T., Yamashiro Y., Uechi Y., Endo S., Suzuki T., Kariya K. Biochem. Biophys. Res. Commun. 377:573-578(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH RAP2A, SUBCELLULAR LOCATION.
[10]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-763, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Phosphoproteome of resting human platelets." Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A. J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-763, MASS SPECTROMETRY. Tissue: Platelet.
[12]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-641; SER-763 AND SER-782, VARIANT [LARGE SCALE ANALYSIS] LEU-775, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-763; SER-777; SER-778 AND SER-782, VARIANT [LARGE SCALE ANALYSIS] LEU-775, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[14]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-763, VARIANT [LARGE SCALE ANALYSIS] LEU-775, MASS SPECTROMETRY.
[15]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-778, VARIANT [LARGE SCALE ANALYSIS] LEU-775, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[16]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-763, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[17]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[18]	"Smad inhibition by the Ste20 kinase Misshapen." Kaneko S., Chen X., Lu P., Yao X., Wright T.G., Rajurkar M., Kariya K., Mao J., Ip Y.T., Xu L. Proc. Natl. Acad. Sci. U.S.A. 108:11127-11132(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[19]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-763, MASS SPECTROMETRY.
[20]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] THR-514; ILE-863; VAL-1010 AND VAL-1200.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB041926 mRNA. Translation: BAA94838.1. AB035698 mRNA. Translation: BAA90753.1. AY775058 mRNA. Translation: AAV41830.1. AC233723 Genomic DNA. No translation available. CH471108 Genomic DNA. Translation: EAW90401.1. CH471108 Genomic DNA. Translation: EAW90403.1. CH471108 Genomic DNA. Translation: EAW90399.1. CH471108 Genomic DNA. Translation: EAW90400.1. CH471108 Genomic DNA. Translation: EAW90402.1. BC034673 mRNA. Translation: AAH34673.1.
IPI	IPI00166680. IPI00218498. IPI00955938.
RefSeq	NP_001020108.1. NM_001024937.3. NP_056531.1. NM_015716.4. NP_722549.2. NM_153827.4. NP_733763.1. NM_170663.4.
UniGene	Hs.443417.
3D structure databases
ProteinModelPortal	Q8N4C8.
SMR	Q8N4C8. Positions 13-373.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8N4C8. 2 interactions.
STRING	9606.ENSP00000347427.
PTM databases
PhosphoSite	Q8N4C8.
Polymorphism databases
DMDM	296437370.
Proteomic databases
PaxDb	Q8N4C8.
PRIDE	Q8N4C8.
Protocols and materials databases
DNASU	50488.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000347992; ENSP00000269296; ENSG00000141503. ENST00000355280; ENSP00000347427; ENSG00000141503. ENST00000453408; ENSP00000406487; ENSG00000141503.
GeneID	50488.
KEGG	hsa:50488.
UCSC	uc010vsk.2. human. uc010vsl.2. human. uc010vsn.2. human.
Organism-specific databases
CTD	50488.
GeneCards	GC17P004736.
H-InvDB	HIX0013452.
HGNC	HGNC:17565. MINK1.
HPA	HPA056296.
MIM	609426. gene.
neXtProt	NX_Q8N4C8.
PharmGKB	PA134910641.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000290708.
HOVERGEN	HBG036506.
InParanoid	Q8N4C8.
KO	K04413.
OMA	GTQTPYG.
Gene expression databases
ArrayExpress	Q8N4C8.
Bgee	Q8N4C8.
CleanEx	HS_MINK1.
Genevestigator	Q8N4C8.
GermOnline	ENSG00000141503. Homo sapiens.
Family and domain databases
InterPro	IPR001180. Citron. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00780. CNH. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00036. CNH. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS50219. CNH. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL5518.
ChiTaRS	MINK1. human.
GenomeRNAi	50488.
NextBio	53052.
SOURCE	Search...

Entry information

Entry name

MINK1_HUMAN

Accession

Primary (citable) accession number: Q8N4C8
Secondary accession number(s): D3DTK3 , D3DTK4, Q5U8Z0, Q9P1X1, Q9P2R8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.