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Protein

Polypeptide N-acetylgalactosaminyltransferase 4

Gene

GALNT4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a highest activity toward Muc7, EA2 and Muc2, with a lowest activity than GALNT2. Glycosylates 'Thr-57' of SELPLG.

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.1 Publication

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei175SubstrateBy similarity1
Binding sitei204SubstrateBy similarity1
Metal bindingi227ManganeseBy similarity1
Metal bindingi229ManganeseBy similarity1
Binding sitei334SubstrateBy similarity1
Metal bindingi362ManganeseBy similarity1
Binding sitei370SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: ProtInc
  • O-glycan processing Source: Reactome

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandLectin, Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.41. 2681.
ReactomeiR-HSA-913709. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 4 (EC:2.4.1.41)
Alternative name(s):
Polypeptide GalNAc transferase 4
Short name:
GalNAc-T4
Short name:
pp-GaNTase 4
Protein-UDP acetylgalactosaminyltransferase 4
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4
Gene namesi
Name:GALNT4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000257594.3.
HGNCiHGNC:4126. GALNT4.
MIMi603565. gene.
neXtProtiNX_Q8N4A0.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 35Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
Topological domaini36 – 578LumenalSequence analysisAdd BLAST543

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi459D → H: Affects the glycopeptide specificity and abolishes ability to glycosylate Muc1, Muc2 and Muc5AC. 1 Publication1

Organism-specific databases

DisGeNETi100528030.
8693.
OpenTargetsiENSG00000257594.
PharmGKBiPA28539.

Polymorphism and mutation databases

BioMutaiGALNT4.
DMDMi338817878.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000591081 – 578Polypeptide N-acetylgalactosaminyltransferase 4Add BLAST578

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi124 ↔ 357PROSITE-ProRule annotation
Disulfide bondi348 ↔ 421PROSITE-ProRule annotation
Disulfide bondi457 ↔ 477PROSITE-ProRule annotation
Glycosylationi471N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi503 ↔ 518PROSITE-ProRule annotation
Disulfide bondi547 ↔ 565PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ8N4A0.
PaxDbiQ8N4A0.
PeptideAtlasiQ8N4A0.
PRIDEiQ8N4A0.

PTM databases

iPTMnetiQ8N4A0.
PhosphoSitePlusiQ8N4A0.
SwissPalmiQ8N4A0.

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in mucous cells.1 Publication

Gene expression databases

BgeeiENSG00000257594.
CleanExiHS_GALNT4.
GenevisibleiQ8N4A0. HS.

Organism-specific databases

HPAiHPA076116.

Interactioni

Protein-protein interaction databases

BioGridi114240. 9 interactors.
1529357. 1 interactor.
STRINGi9606.ENSP00000436604.

Structurei

Secondary structure

1578
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi62 – 64Combined sources3
Helixi76 – 78Combined sources3
Helixi87 – 100Combined sources14
Helixi104 – 107Combined sources4
Helixi122 – 125Combined sources4
Turni131 – 133Combined sources3
Beta strandi137 – 145Combined sources9
Helixi148 – 161Combined sources14
Turni164 – 166Combined sources3
Beta strandi167 – 174Combined sources8
Helixi180 – 182Combined sources3
Helixi184 – 190Combined sources7
Beta strandi196 – 200Combined sources5
Helixi207 – 217Combined sources11
Beta strandi220 – 225Combined sources6
Beta strandi228 – 232Combined sources5
Helixi238 – 246Combined sources9
Beta strandi250 – 259Combined sources10
Turni261 – 263Combined sources3
Beta strandi275 – 278Combined sources4
Beta strandi284 – 287Combined sources4
Helixi291 – 296Combined sources6
Beta strandi314 – 317Combined sources4
Helixi318 – 323Combined sources6
Beta strandi333 – 335Combined sources3
Helixi338 – 347Combined sources10
Beta strandi351 – 363Combined sources13
Helixi376 – 386Combined sources11
Helixi388 – 390Combined sources3
Helixi391 – 397Combined sources7
Helixi399 – 403Combined sources5
Helixi410 – 418Combined sources9
Helixi424 – 430Combined sources7
Beta strandi444 – 453Combined sources10
Beta strandi456 – 459Combined sources4
Beta strandi473 – 476Combined sources4
Helixi482 – 484Combined sources3
Beta strandi486 – 488Combined sources3
Beta strandi494 – 496Combined sources3
Beta strandi498 – 500Combined sources3
Beta strandi502 – 505Combined sources4
Beta strandi514 – 517Combined sources4
Helixi527 – 529Combined sources3
Beta strandi539 – 541Combined sources3
Turni542 – 545Combined sources4
Beta strandi546 – 552Combined sources7
Beta strandi558 – 563Combined sources6
Helixi570 – 572Combined sources3
Beta strandi574 – 577Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5NQAX-ray1.90A/B1-578[»]
ProteinModelPortaliQ8N4A0.
SMRiQ8N4A0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini444 – 577Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni134 – 243Catalytic subdomain AAdd BLAST110
Regioni303 – 365Catalytic subdomain BAdd BLAST63

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain directs the glycopeptide specificity. It is required in the glycopeptide specificity of enzyme activity but not for activity with naked peptide substrates, suggesting that it triggers the catalytic domain to act on GalNAc-glycopeptide substrates.1 Publication

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3736. Eukaryota.
ENOG410XPMK. LUCA.
GeneTreeiENSGT00760000118828.
HOGENOMiHOG000038227.
HOVERGENiHBG051699.
InParanoidiQ8N4A0.
KOiK00710.
OMAiISLHRHI.
OrthoDBiEOG091G085O.
PhylomeDBiQ8N4A0.
TreeFamiTF352660.

Family and domain databases

CDDicd00161. RICIN. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiView protein in InterPro
IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR035992. Ricin_B-like_lectins.
IPR000772. Ricin_B_lectin.
PfamiView protein in Pfam
PF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
SMARTiView protein in SMART
SM00458. RICIN. 1 hit.
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiView protein in PROSITE
PS50231. RICIN_B_LECTIN. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N4A0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVRWTWAGK SCLLLAFLTV AYIFVELLVS TFHASAGAGR ARELGSRRLS
60 70 80 90 100
DLQKNTEDLS RPLYKKPPAD SRALGEWGKA SKLQLNEDEL KQQEELIERY
110 120 130 140 150
AINIYLSDRI SLHRHIEDKR MYECKSQKFN YRTLPTTSVI IAFYNEAWST
160 170 180 190 200
LLRTIHSVLE TSPAVLLKEI ILVDDLSDRV YLKTQLETYI SNLDRVRLIR
210 220 230 240 250
TNKREGLVRA RLIGATFATG DVLTFLDCHC ECNSGWLEPL LERIGRDETA
260 270 280 290 300
VVCPVIDTID WNTFEFYMQI GEPMIGGFDW RLTFQWHSVP KQERDRRISR
310 320 330 340 350
IDPIRSPTMA GGLFAVSKKY FQYLGTYDTG MEVWGGENLE LSFRVWQCGG
360 370 380 390 400
KLEIHPCSHV GHVFPKRAPY ARPNFLQNTA RAAEVWMDEY KEHFYNRNPP
410 420 430 440 450
ARKEAYGDIS ERKLLRERLR CKSFDWYLKN VFPNLHVPED RPGWHGAIRS
460 470 480 490 500
RGISSECLDY NSPDNNPTGA NLSLFGCHGQ GGNQFFEYTS NKEIRFNSVT
510 520 530 540 550
ELCAEVPEQK NYVGMQNCPK DGFPVPANII WHFKEDGTIF HPHSGLCLSA
560 570
YRTPEGRPDV QMRTCDALDK NQIWSFEK
Length:578
Mass (Da):66,666
Last modified:June 28, 2011 - v2
Checksum:i6EEEF502A40CBD2E
GO
Isoform 2 (identifier: Q8N4A0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-120: MAVRWTWAGK...SLHRHIEDKR → MAWCVATADP...WTSAPTASNL
     121-292: Missing.

Note: No experimental confirmation available.
Show »
Length:406
Mass (Da):45,702
Checksum:iEB89E46BF360847D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11S → T in CAA69875 (PubMed:9804815).Curated1
Sequence conflicti227D → Y in CAA69875 (PubMed:9804815).Curated1
Sequence conflicti247D → Y in CAA69875 (PubMed:9804815).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06525751D → G1 PublicationCorresponds to variant dbSNP:rs17853610Ensembl.1
Natural variantiVAR_019576270I → T1 PublicationCorresponds to variant dbSNP:rs2230281Ensembl.1
Natural variantiVAR_019577506V → I2 PublicationsCorresponds to variant dbSNP:rs2230283Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0450091 – 120MAVRW…IEDKR → MAWCVATADPAHTSRPLFTG LAVSRGSAGHAWSAGFDWAA VVVVTGRRCRSGQTVPGAAR SPLLPHPLPSPLRVPPPTGA LGRPLPRWPQPRRTPFWSVI SKATKLRSPPWTSAPTASNL in isoform 2. 1 PublicationAdd BLAST120
Alternative sequenceiVSP_045010121 – 292Missing in isoform 2. 1 PublicationAdd BLAST172

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08564 Genomic DNA. Translation: CAA69875.1.
AK297677 mRNA. Translation: BAG60038.1.
AK312870 mRNA. Translation: BAG35722.1.
AC010201 Genomic DNA. No translation available.
AC025034 Genomic DNA. No translation available.
BC036390 mRNA. Translation: AAH36390.1.
CCDSiCCDS53817.1. [Q8N4A0-1]
RefSeqiNP_001186711.1. NM_001199782.1. [Q8N4A0-2]
NP_003765.2. NM_003774.4. [Q8N4A0-1]
UniGeneiHs.25130.

Genome annotation databases

EnsembliENST00000529983; ENSP00000436604; ENSG00000257594. [Q8N4A0-1]
GeneIDi100528030.
8693.
KEGGihsa:100528030.
hsa:8693.
UCSCiuc001tbd.4. human. [Q8N4A0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiGALT4_HUMAN
AccessioniPrimary (citable) accession number: Q8N4A0
Secondary accession number(s): B2R775, B4DMX6, O00208
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: June 28, 2011
Last modified: March 28, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome