Q8N448 (LNX2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ligand of Numb protein X 2

Alternative name(s):
Numb-binding protein 2
PDZ domain-containing RING finger protein 1

Gene names

Name:	LNX2
Synonyms:	PDZRN1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with the phosphotyrosine interaction domain of NUMB By similarity.
Domain	The NPXY motif is required for the interaction with the PID domain of NUMB. It is however not sufficient.
Sequence similarities	Contains 4 PDZ (DHR) domains. Contains 1 RING-type zinc finger.
Sequence caution	The sequence BAD18754.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 690

690

Ligand of Numb protein X 2

PRO_0000055915

Regions

Domain

233 – 318

86

PDZ 1

Domain

339 – 422

84

PDZ 2

Domain

468 – 554

87

PDZ 3

Domain

600 – 688

89

PDZ 4

Zinc finger

50 – 88

39

RING-type

Motif

208 – 211

4

NPXY motif

Natural variations

Natural variant

198

1

S → P.
Corresponds to variant rs8002697 [ dbSNP | Ensembl ].

VAR_024612

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

690

Helix Strand Turn

Details...

Beta strand

336 – 343

8

Beta strand

354 – 356

3

Beta strand

365 – 368

4

Turn

375 – 377

3

Beta strand

386 – 390

5

Helix

400 – 409

10

Beta strand

413 – 420

8

Sequences

	Sequence		Length	Mass (Da)
	Q8N448 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: A3F941A477721F1D	FASTA	690	76,004
	10 20 30 40 50 60 MGTTSDEMVS VEQTSSSSLN PLCFECGQQH WTRENHLYNY QNEVDDDLVC HICLQPLLQP 70 80 90 100 110 120 LDTPCGHTFC YKCLRNFLQE KDFCPLDRKR LHFKLCKKSS ILVHKLLDKL LVLCPFSSVC 130 140 150 160 170 180 KDVMQRCDLE AHLKNRCPGA SHRRVALERR KTSRTQAEIE NENGPTLLDP AGTLSPEADC 190 200 210 220 230 240 LGTGAVPVER HLTSASLSTW SEEPGLDNPA FEESAGADTT QQPLSLPEGE ITTIEIHRSN 250 260 270 280 290 300 PYIQLGISIV GGNETPLINI VIQEVYRDGV IARDGRLLAG DQILQVNNYN ISNVSHNYAR 310 320 330 340 350 360 AVLSQPCNTL HLTVLRERRF GNRAHNHSDS NSPREEIFQV ALHKRDSGEQ LGIKLVRRTD 370 380 390 400 410 420 EPGVFILDLL EGGLAAQDGR LSSNDRVLAI NGHDLKYGTP ELAAQIIQAS GERVNLTIAR 430 440 450 460 470 480 PGKPQPGNTI REAGNHSSSS QHHTPPPYYS RPSSHKDLTQ CVTCQEKHIT VKKEPHESLG 490 500 510 520 530 540 MTVAGGRGSK SGELPIFVTS VPPHGCLARD GRIKRGDVLL NINGIDLTNL SHSEAVAMLK 550 560 570 580 590 600 ASAASPAVAL KALEVQIVEE ATQNAEEQPS TFSENEYDAS WSPSWVMWLG LPSTLHSCHD 610 620 630 640 650 660 IVLRRSYLGS WGFSIVGGYE ENHTNQPFFI KTIVLGTPAY YDGRLKCGDM IVAVNGLSTV 670 680 690 GMSHSALVPM LKEQRNKVTL TVICWPGSLV

References

[1]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed: 15057823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Melanoma.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 522-690. Tissue: Colon.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AL138699 Genomic DNA. Translation: CAH73446.1.
BC036755 mRNA. Translation: AAH36755.1.
AK172771 mRNA. Translation: BAD18754.1. Different initiation.

IPI

IPI00171047.

RefSeq

NP_699202.1. NM_153371.3.

UniGene

Hs.132359.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VWR	X-ray	1.30	A	336-424	[»]

ProteinModelPortal

Q8N448.

SMR

Q8N448. Positions 38-138, 223-687.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q8N448. 15 interactions.

STRING

Q8N448.

Polymorphism databases

DMDM

29840784.

Proteomic databases

PeptideAtlas

Q8N448.

PRIDE

Q8N448.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000316334; ENSP00000325929; ENSG00000139517.

GeneID

222484.

KEGG

hsa:222484.

UCSC

uc001url.2. human.

Organism-specific databases

CTD

222484.

GeneCards

GC13M028120.

HGNC

HGNC:20421. LNX2.

HPA

HPA040698.

MIM

609733. gene.

neXtProt

NX_Q8N448.

PharmGKB

PA134899015.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG18073.

GeneTree

ENSGT00570000078792.

HOGENOM

HBG444181.

HOVERGEN

HBG039539.

InParanoid

Q8N448.

OMA

ENHLYNY.

OrthoDB

EOG4ZW59N.

PhylomeDB

Q8N448.

Gene expression databases

ArrayExpress

Q8N448.

Bgee

Q8N448.

CleanEx

HS_LNX2.

Genevestigator

Q8N448.

GermOnline

ENSG00000139517. Homo sapiens.

Family and domain databases

InterPro

IPR001478. PDZ/DHR/GLGF.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]

Gene3D

G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.

Pfam

PF00595. PDZ. 4 hits.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]

SMART

SM00228. PDZ. 4 hits.
SM00184. RING. 1 hit.
[Graphical view]

SUPFAM

SSF50156. PDZ. 4 hits.

PROSITE

PS50106. PDZ. 4 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

91602.

SOURCE

Search...

Entry information

Entry name

LNX2_HUMAN

Accession

Primary (citable) accession number: Q8N448
Secondary accession number(s): Q5W0P0, Q6ZMH2, Q96SH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 28, 2003
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.