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Protein

Translation factor GUF1, mitochondrial

Gene

GUF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.UniRule annotation

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 82GTPUniRule annotation8
Nucleotide bindingi140 – 144GTPUniRule annotation5
Nucleotide bindingi194 – 197GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Translation factor GUF1, mitochondrialUniRule annotation (EC:3.6.5.-)
Alternative name(s):
Elongation factor 4 homologUniRule annotation
Short name:
EF-4UniRule annotation
GTPase GUF1UniRule annotation
Ribosomal back-translocaseUniRule annotation
Gene namesi
Name:GUF1UniRule annotation
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:25799. GUF1.

Subcellular locationi

  • Mitochondrion inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Matrix side UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000151806.
PharmGKBiPA143485485.

Polymorphism and mutation databases

BioMutaiGUF1.
DMDMi74728811.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 49MitochondrionUniRule annotationAdd BLAST49
ChainiPRO_000025625150 – 669Translation factor GUF1, mitochondrialAdd BLAST620

Proteomic databases

EPDiQ8N442.
MaxQBiQ8N442.
PaxDbiQ8N442.
PeptideAtlasiQ8N442.
PRIDEiQ8N442.

PTM databases

iPTMnetiQ8N442.
PhosphoSitePlusiQ8N442.

Expressioni

Gene expression databases

BgeeiENSG00000151806.
CleanExiHS_GUF1.
ExpressionAtlasiQ8N442. baseline and differential.
GenevisibleiQ8N442. HS.

Organism-specific databases

HPAiHPA003934.
HPA024222.

Interactioni

Protein-protein interaction databases

BioGridi121939. 8 interactors.
IntActiQ8N442. 1 interactor.
STRINGi9606.ENSP00000281543.

Structurei

3D structure databases

ProteinModelPortaliQ8N442.
SMRiQ8N442.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini66 – 247tr-type GAdd BLAST182

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0462. Eukaryota.
COG0481. LUCA.
GeneTreeiENSGT00550000074940.
HOGENOMiHOG000020624.
HOVERGENiHBG081580.
InParanoidiQ8N442.
OMAiFAGIYPV.
OrthoDBiEOG091G03BP.
PhylomeDBiQ8N442.
TreeFamiTF314751.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA. 1 hit.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8N442-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWTLVGRGWG CARALAPRAT GAALLVAPGP RSAPTLGAAP ESWATDRLYS
60 70 80 90 100
SAEFKEKLDM SRFPVENIRN FSIVAHVDHG KSTLADRLLE LTGTIDKTKN
110 120 130 140 150
NKQVLDKLQV ERERGITVKA QTASLFYNCE GKQYLLNLID TPGHVDFSYE
160 170 180 190 200
VSRSLSACQG VLLVVDANEG IQAQTVANFF LAFEAQLSVI PVINKIDLKN
210 220 230 240 250
ADPERVENQI EKVFDIPSDE CIKISAKLGT NVESVLQAII ERIPPPKVHR
260 270 280 290 300
KNPLRALVFD STFDQYRGVI ANVALFDGVV SKGDKIVSAH TQKTYEVNEV
310 320 330 340 350
GVLNPNEQPT HKLYAGQVGY LIAGMKDVTE AQIGDTLCLH KQPVEPLPGF
360 370 380 390 400
KSAKPMVFAG MYPLDQSEYN NLKSAIEKLT LNDSSVTVHR DSSLALGAGW
410 420 430 440 450
RLGFLGLLHM EVFNQRLEQE YNASVILTTP TVPYKAVLSS SKLIKEHREK
460 470 480 490 500
EITIINPAQF PDKSKVTEYL EPVVLGTIIT PDEYTGKIMM LCEARRAVQK
510 520 530 540 550
NMIFIDQNRV MLKYLFPLNE IVVDFYDSLK SLSSGYASFD YEDAGYQTAE
560 570 580 590 600
LVKMDILLNG NTVEELVTVV HKDKAHSIGK AICERLKDSL PRQLFEIAIQ
610 620 630 640 650
AAIGSKIIAR ETVKAYRKNV LAKCYGGDIT RKMKLLKRQA EGKKKLRKIG
660
NVEVPKDAFI KVLKTQSSK
Length:669
Mass (Da):74,328
Last modified:October 1, 2002 - v1
Checksum:i428892437C91ED36
GO

Sequence cautioni

The sequence BAB15090 differs from that shown. Reason: Frameshift at position 646.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85A → T in BAB14507 (PubMed:14702039).Curated1
Sequence conflicti417L → P in BAB14507 (PubMed:14702039).Curated1
Sequence conflicti625Y → C in BAB14507 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02889558L → P.1 PublicationCorresponds to variant rs6447368dbSNPEnsembl.1
Natural variantiVAR_028896329T → I.Corresponds to variant rs10470742dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023282 mRNA. Translation: BAB14507.1.
AK025248 mRNA. Translation: BAB15090.1. Sequence problems.
BC036768 mRNA. Translation: AAH36768.1.
CCDSiCCDS3468.1.
RefSeqiNP_068746.2. NM_021927.2.
UniGeneiHs.546419.

Genome annotation databases

EnsembliENST00000281543; ENSP00000281543; ENSG00000151806.
GeneIDi60558.
KEGGihsa:60558.
UCSCiuc003gww.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023282 mRNA. Translation: BAB14507.1.
AK025248 mRNA. Translation: BAB15090.1. Sequence problems.
BC036768 mRNA. Translation: AAH36768.1.
CCDSiCCDS3468.1.
RefSeqiNP_068746.2. NM_021927.2.
UniGeneiHs.546419.

3D structure databases

ProteinModelPortaliQ8N442.
SMRiQ8N442.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121939. 8 interactors.
IntActiQ8N442. 1 interactor.
STRINGi9606.ENSP00000281543.

PTM databases

iPTMnetiQ8N442.
PhosphoSitePlusiQ8N442.

Polymorphism and mutation databases

BioMutaiGUF1.
DMDMi74728811.

Proteomic databases

EPDiQ8N442.
MaxQBiQ8N442.
PaxDbiQ8N442.
PeptideAtlasiQ8N442.
PRIDEiQ8N442.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000281543; ENSP00000281543; ENSG00000151806.
GeneIDi60558.
KEGGihsa:60558.
UCSCiuc003gww.5. human.

Organism-specific databases

CTDi60558.
GeneCardsiGUF1.
HGNCiHGNC:25799. GUF1.
HPAiHPA003934.
HPA024222.
neXtProtiNX_Q8N442.
OpenTargetsiENSG00000151806.
PharmGKBiPA143485485.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0462. Eukaryota.
COG0481. LUCA.
GeneTreeiENSGT00550000074940.
HOGENOMiHOG000020624.
HOVERGENiHBG081580.
InParanoidiQ8N442.
OMAiFAGIYPV.
OrthoDBiEOG091G03BP.
PhylomeDBiQ8N442.
TreeFamiTF314751.

Miscellaneous databases

ChiTaRSiGUF1. human.
GenomeRNAii60558.
PROiQ8N442.

Gene expression databases

BgeeiENSG00000151806.
CleanExiHS_GUF1.
ExpressionAtlasiQ8N442. baseline and differential.
GenevisibleiQ8N442. HS.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA. 1 hit.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUF1_HUMAN
AccessioniPrimary (citable) accession number: Q8N442
Secondary accession number(s): Q5XKM8, Q9H710, Q9H8U4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.