Q8N3X1 (FNBP4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formin-binding protein 4

Alternative name(s):
Formin-binding protein 30

Gene names

Name:	FNBP4
Synonyms:	FBP30, KIAA1014

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1017 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Binds FMN1. Interacts with the Arg/Gly-rich-flanked Pro-rich of KHDRBS1/SAM68. Arginine methylation in these regions has no effect on this binding. Ref.7
Domain	These WW domains interact with Arg/Gly-rich-flanked Pro-rich domains found in several WW domain-binding proteins (WBPs). The N-terminal WW domain has the greater ligand-binding ability By similarity.
Sequence similarities	Contains 2 WW domains.
Sequence caution	The sequence BAA76858.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from direct assay. Source: HPA
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N3X1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N3X1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     73-73: E → EGK

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1017	1017	Formin-binding protein 4		PRO_0000289863
Regions
	Domain	214 – 248	35	WW 1
	Domain	595 – 629	35	WW 2
	Compositional bias	630 – 635	6	Poly-Glu
	Compositional bias	701 – 731	31	Pro-rich
	Compositional bias	905 – 925	21	Pro-rich
Amino acid modifications
	Modified residue	18	1	Phosphoserine Ref.8 Ref.12
	Modified residue	116	1	Phosphoserine Ref.10 Ref.12
	Modified residue	172	1	Phosphothreonine Ref.8
	Modified residue	432	1	Phosphoserine Ref.9 Ref.10 Ref.11 Ref.12 Ref.14
	Modified residue	435	1	Phosphoserine Ref.11
	Modified residue	464	1	Phosphoserine Ref.12
	Modified residue	479	1	Phosphothreonine Ref.12
	Modified residue	499	1	Phosphoserine Ref.11
	Modified residue	508	1	Phosphoserine Ref.11 Ref.12 Ref.14
	Modified residue	963	1	Phosphoserine Ref.10
	Modified residue	964	1	Phosphoserine Ref.10
	Modified residue	965	1	Phosphoserine Ref.10
Natural variations
	Alternative sequence	73	1	E → EGK in isoform 2.		VSP_040292
	Natural variant	125	1	E → G. Corresponds to variant rs34962598 [ dbSNP | Ensembl ].		VAR_032623
	Natural variant	794	1	T → A. Corresponds to variant rs35040940 [ dbSNP | Ensembl ].		VAR_032624
Experimental info
	Sequence conflict	55 – 56	2	Missing in BAA76858. Ref.1
	Sequence conflict	55 – 56	2	Missing in AAH37404. Ref.4
	Sequence conflict	55 – 56	2	Missing in CAB70761. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 30, 2010. Version 3. Checksum: 510DC8011B5973AD	FASTA	1,017	110,266
	10 20 30 40 50 60 MGKKSRAVPG RRPILQLSPP GPRGSTPGRD PEPEPDTEPD STAAVPSQPA PSAATTTTTA 70 80 90 100 110 120 VTAAAASDDS PSEDEQEAVQ EVPRVVQNPP KPVMTTRPTA VKATGGLCLL GAYADSDDDD 130 140 150 160 170 180 NDVSEKLAQS KETNGNQSTD IDSTLANFLA EIDAITAPQP AAPVGASAPP PTPPRPEPKE 190 200 210 220 230 240 AATSTLSSST SNGTDSTQTS GWQYDTQCSL AGVGIEMGDW QEVWDENTGC YYYWNTQTNE 250 260 270 280 290 300 VTWELPQYLA TQVQGLQHYQ PSSVPGAETS FVVNTDIYSK EKTISVSSSK SGPVIAKREV 310 320 330 340 350 360 KKEVNEGIQA LSNSEEEKKG VAASLLAPLL PEGIKEEEER WRRKVICKEE PVSEVKETST 370 380 390 400 410 420 TVEEATTIVK PQEIMLDNIE DPSQEDLCSV VQSGESEEEE EQDTLELELV LERKKAELRA 430 440 450 460 470 480 LEEGDGSVSG SSPRSDISQP ASQDGMRRLM SKRGKWKMFV RATSPESTSR SSSKTGRDTP 490 500 510 520 530 540 ENGETAIGAE NSEKIDENSD KEMEVEESPE KIKVQTTPKV EEEQDLKFQI GELANTLTSK 550 560 570 580 590 600 FEFLGINRQS ISNFHVLLLQ TETRIADWRE GALNGNYLKR KLQDAAEQLK QYEINATPKG 610 620 630 640 650 660 WSCHWDRDHR RYFYVNEQSG ESQWEFPDGE EEEEESQAQE NRDETLAKQT LKDKTGTDSN 670 680 690 700 710 720 STESSETSTG SLCKESFSGQ VSSSSLMPLT PFWTLLQSNV PVLQPPLPLE MPPPPPPPPE 730 740 750 760 770 780 SPPPPPPPPP PAEDGEIQEV EMEDEGSEEP PAPGTEEDTP LKPSAQTTVV TSQSSVDSTI 790 800 810 820 830 840 SSSSSTKGIK RKATEISTAV VQRSATIGSS PVLYSQSAIA TGHQAAGIGN QATGIGHQTI 850 860 870 880 890 900 PVSLPAAGMG HQARGMSLQS NYLGLAAAPA IMSYAECSVP IGVTAPSLQP VQARGAVPTA 910 920 930 940 950 960 TIIEPPPPPP PPPPPPPPAP KMPPPEKTKK GRKDKAKKSK TKMPSLVKKW QSIQRELDEE 970 980 990 1000 1010 DNSSSSEEDR ESTAQKRIEE WKQQQLVSGM AERNANFEAL PEDWRARLKR RKMAPNT
	Isoform 2 [UniParc]. Checksum: 2D60B6B14CC4CE16	FASTA	1,019	110,451
	10 20 30 40 50 60 MGKKSRAVPG RRPILQLSPP GPRGSTPGRD PEPEPDTEPD STAAVPSQPA PSAATTTTTA 70 80 90 100 110 120 VTAAAASDDS PSEGKDEQEA VQEVPRVVQN PPKPVMTTRP TAVKATGGLC LLGAYADSDD 130 140 150 160 170 180 DDNDVSEKLA QSKETNGNQS TDIDSTLANF LAEIDAITAP QPAAPVGASA PPPTPPRPEP 190 200 210 220 230 240 KEAATSTLSS STSNGTDSTQ TSGWQYDTQC SLAGVGIEMG DWQEVWDENT GCYYYWNTQT 250 260 270 280 290 300 NEVTWELPQY LATQVQGLQH YQPSSVPGAE TSFVVNTDIY SKEKTISVSS SKSGPVIAKR 310 320 330 340 350 360 EVKKEVNEGI QALSNSEEEK KGVAASLLAP LLPEGIKEEE ERWRRKVICK EEPVSEVKET 370 380 390 400 410 420 STTVEEATTI VKPQEIMLDN IEDPSQEDLC SVVQSGESEE EEEQDTLELE LVLERKKAEL 430 440 450 460 470 480 RALEEGDGSV SGSSPRSDIS QPASQDGMRR LMSKRGKWKM FVRATSPEST SRSSSKTGRD 490 500 510 520 530 540 TPENGETAIG AENSEKIDEN SDKEMEVEES PEKIKVQTTP KVEEEQDLKF QIGELANTLT 550 560 570 580 590 600 SKFEFLGINR QSISNFHVLL LQTETRIADW REGALNGNYL KRKLQDAAEQ LKQYEINATP 610 620 630 640 650 660 KGWSCHWDRD HRRYFYVNEQ SGESQWEFPD GEEEEEESQA QENRDETLAK QTLKDKTGTD 670 680 690 700 710 720 SNSTESSETS TGSLCKESFS GQVSSSSLMP LTPFWTLLQS NVPVLQPPLP LEMPPPPPPP 730 740 750 760 770 780 PESPPPPPPP PPPAEDGEIQ EVEMEDEGSE EPPAPGTEED TPLKPSAQTT VVTSQSSVDS 790 800 810 820 830 840 TISSSSSTKG IKRKATEIST AVVQRSATIG SSPVLYSQSA IATGHQAAGI GNQATGIGHQ 850 860 870 880 890 900 TIPVSLPAAG MGHQARGMSL QSNYLGLAAA PAIMSYAECS VPIGVTAPSL QPVQARGAVP 910 920 930 940 950 960 TATIIEPPPP PPPPPPPPPP APKMPPPEKT KKGRKDKAKK SKTKMPSLVK KWQSIQRELD 970 980 990 1000 1010 EEDNSSSSEE DRESTAQKRI EEWKQQQLVS GMAERNANFE ALPEDWRARL KRRKMAPNT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[2]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Cervix.
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-1017 (ISOFORM 1). Tissue: Testis.
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 457-1017 (ISOFORMS 1/2).
[7]	"Arginine methylation inhibits the binding of proline-rich ligands to Src homology 3, but not WW, domains." Bedford M.T., Frankel A., Yaffe M.B., Clarke S., Leder P., Richard S. J. Biol. Chem. 275:16030-16036(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH KHDRBS1.
[8]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18 AND THR-172, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-432, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-116; SER-432; SER-963; SER-964 AND SER-965, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-432; SER-435; SER-499 AND SER-508, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[12]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-116; SER-432; SER-464; THR-479 AND SER-508, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-432 AND SER-508, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB023231 mRNA. Translation: BAA76858.2. Different initiation. AC021443 Genomic DNA. No translation available. BC037404 mRNA. Translation: AAH37404.1. AL137480 mRNA. Translation: CAB70761.1. AK022987 mRNA. Translation: BAB14348.1.
IPI	IPI00170778. IPI00972964.
PIR	T46422.
RefSeq	NP_056123.2. NM_015308.2.
UniGene	Hs.6834.
3D structure databases
ProteinModelPortal	Q8N3X1.
SMR	Q8N3X1. Positions 217-252, 597-630.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8N3X1. 2 interactions.
MINT	MINT-127221.
STRING	9606.ENSP00000263773.
PTM databases
PhosphoSite	Q8N3X1.
Polymorphism databases
DMDM	148886619.
Proteomic databases
PaxDb	Q8N3X1.
PRIDE	Q8N3X1.
Protocols and materials databases
DNASU	23360.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000263773; ENSP00000263773; ENSG00000109920.
GeneID	23360.
KEGG	hsa:23360.
UCSC	uc001ngj.3. human.
Organism-specific databases
CTD	23360.
GeneCards	GC11M047805.
H-InvDB	HIX0201592.
HGNC	HGNC:19752. FNBP4.
HPA	HPA039485. HPA040029.
neXtProt	NX_Q8N3X1.
PharmGKB	PA134971679.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG46362.
HOGENOM	HOG000168238.
HOVERGEN	HBG066308.
InParanoid	Q8N3X1.
OMA	PSQEDLC.
OrthoDB	EOG42BX80.
Gene expression databases
ArrayExpress	Q8N3X1.
Bgee	Q8N3X1.
CleanEx	HS_FNBP4.
Genevestigator	Q8N3X1.
Family and domain databases
InterPro	IPR001202. WW_dom. [Graphical view]
Pfam	PF00397. WW. 2 hits. [Graphical view]
SMART	SM00456. WW. 2 hits. [Graphical view]
SUPFAM	SSF51045. WW_Rsp5_WWP. 2 hits.
PROSITE	PS01159. WW_DOMAIN_1. 1 hit. PS50020. WW_DOMAIN_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	FNBP4. human.
GenomeRNAi	23360.
NextBio	45391.
PMAP-CutDB	Q8N3X1.

Entry information

Entry name

FNBP4_HUMAN

Accession

Primary (citable) accession number: Q8N3X1
Secondary accession number(s): Q9H985, Q9NT81, Q9Y2L7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	November 30, 2010
Last modified:	May 1, 2013
This is version 85 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.