Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8N3X1 (FNBP4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formin-binding protein 4
Alternative name(s):
Formin-binding protein 30
Gene names
Name:FNBP4
Synonyms:FBP30, KIAA1014
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1017 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Binds FMN1. Interacts with the Arg/Gly-rich-flanked Pro-rich of KHDRBS1/SAM68. Arginine methylation in these regions has no effect on this binding. Ref.7

Domain

These WW domains interact with Arg/Gly-rich-flanked Pro-rich domains found in several WW domain-binding proteins (WBPs). The N-terminal WW domain has the greater ligand-binding ability By similarity.

Sequence similarities

Contains 2 WW domains.

Sequence caution

The sequence BAA76858.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from direct assay. Source: HPA

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 17500595. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HTTP428586EBI-310600,EBI-466029

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8N3X1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8N3X1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     73-73: E → EGK

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10171017Formin-binding protein 4
PRO_0000289863

Regions

Domain214 – 24835WW 1
Domain595 – 62935WW 2
Compositional bias630 – 6356Poly-Glu
Compositional bias701 – 73131Pro-rich
Compositional bias905 – 92521Pro-rich

Amino acid modifications

Modified residue181Phosphoserine Ref.8 Ref.13
Modified residue1161Phosphoserine Ref.10 Ref.13
Modified residue1721Phosphothreonine Ref.8
Modified residue2901N6-acetyllysine By similarity
Modified residue4321Phosphoserine Ref.9 Ref.10 Ref.12 Ref.13 Ref.15
Modified residue4351Phosphoserine Ref.12
Modified residue4641Phosphoserine Ref.13
Modified residue4791Phosphothreonine Ref.13
Modified residue4991Phosphoserine Ref.12
Modified residue5081Phosphoserine Ref.12 Ref.13 Ref.15
Modified residue9631Phosphoserine Ref.10
Modified residue9641Phosphoserine Ref.10
Modified residue9651Phosphoserine Ref.10

Natural variations

Alternative sequence731E → EGK in isoform 2.
VSP_040292
Natural variant1251E → G.
Corresponds to variant rs34962598 [ dbSNP | Ensembl ].
VAR_032623
Natural variant7941T → A.
Corresponds to variant rs35040940 [ dbSNP | Ensembl ].
VAR_032624

Experimental info

Sequence conflict55 – 562Missing in BAA76858. Ref.1
Sequence conflict55 – 562Missing in AAH37404. Ref.4
Sequence conflict55 – 562Missing in CAB70761. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 30, 2010. Version 3.
Checksum: 510DC8011B5973AD

FASTA1,017110,266
        10         20         30         40         50         60 
MGKKSRAVPG RRPILQLSPP GPRGSTPGRD PEPEPDTEPD STAAVPSQPA PSAATTTTTA 

        70         80         90        100        110        120 
VTAAAASDDS PSEDEQEAVQ EVPRVVQNPP KPVMTTRPTA VKATGGLCLL GAYADSDDDD 

       130        140        150        160        170        180 
NDVSEKLAQS KETNGNQSTD IDSTLANFLA EIDAITAPQP AAPVGASAPP PTPPRPEPKE 

       190        200        210        220        230        240 
AATSTLSSST SNGTDSTQTS GWQYDTQCSL AGVGIEMGDW QEVWDENTGC YYYWNTQTNE 

       250        260        270        280        290        300 
VTWELPQYLA TQVQGLQHYQ PSSVPGAETS FVVNTDIYSK EKTISVSSSK SGPVIAKREV 

       310        320        330        340        350        360 
KKEVNEGIQA LSNSEEEKKG VAASLLAPLL PEGIKEEEER WRRKVICKEE PVSEVKETST 

       370        380        390        400        410        420 
TVEEATTIVK PQEIMLDNIE DPSQEDLCSV VQSGESEEEE EQDTLELELV LERKKAELRA 

       430        440        450        460        470        480 
LEEGDGSVSG SSPRSDISQP ASQDGMRRLM SKRGKWKMFV RATSPESTSR SSSKTGRDTP 

       490        500        510        520        530        540 
ENGETAIGAE NSEKIDENSD KEMEVEESPE KIKVQTTPKV EEEQDLKFQI GELANTLTSK 

       550        560        570        580        590        600 
FEFLGINRQS ISNFHVLLLQ TETRIADWRE GALNGNYLKR KLQDAAEQLK QYEINATPKG 

       610        620        630        640        650        660 
WSCHWDRDHR RYFYVNEQSG ESQWEFPDGE EEEEESQAQE NRDETLAKQT LKDKTGTDSN 

       670        680        690        700        710        720 
STESSETSTG SLCKESFSGQ VSSSSLMPLT PFWTLLQSNV PVLQPPLPLE MPPPPPPPPE 

       730        740        750        760        770        780 
SPPPPPPPPP PAEDGEIQEV EMEDEGSEEP PAPGTEEDTP LKPSAQTTVV TSQSSVDSTI 

       790        800        810        820        830        840 
SSSSSTKGIK RKATEISTAV VQRSATIGSS PVLYSQSAIA TGHQAAGIGN QATGIGHQTI 

       850        860        870        880        890        900 
PVSLPAAGMG HQARGMSLQS NYLGLAAAPA IMSYAECSVP IGVTAPSLQP VQARGAVPTA 

       910        920        930        940        950        960 
TIIEPPPPPP PPPPPPPPAP KMPPPEKTKK GRKDKAKKSK TKMPSLVKKW QSIQRELDEE 

       970        980        990       1000       1010 
DNSSSSEEDR ESTAQKRIEE WKQQQLVSGM AERNANFEAL PEDWRARLKR RKMAPNT 

« Hide

Isoform 2 [UniParc].

Checksum: 2D60B6B14CC4CE16
Show »

FASTA1,019110,451

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[2]"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[3]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Cervix.
[5]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-1017 (ISOFORM 1).
Tissue: Testis.
[6]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 457-1017 (ISOFORMS 1/2).
[7]"Arginine methylation inhibits the binding of proline-rich ligands to Src homology 3, but not WW, domains."
Bedford M.T., Frankel A., Yaffe M.B., Clarke S., Leder P., Richard S.
J. Biol. Chem. 275:16030-16036(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH KHDRBS1.
[8]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18 AND THR-172, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-432, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-116; SER-432; SER-963; SER-964 AND SER-965, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-432; SER-435; SER-499 AND SER-508, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[13]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; SER-116; SER-432; SER-464; THR-479 AND SER-508, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[14]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-432 AND SER-508, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB023231 mRNA. Translation: BAA76858.2. Different initiation.
AC021443 Genomic DNA. No translation available.
BC037404 mRNA. Translation: AAH37404.1.
AL137480 mRNA. Translation: CAB70761.1.
AK022987 mRNA. Translation: BAB14348.1.
CCDSCCDS41644.1. [Q8N3X1-1]
PIRT46422.
RefSeqNP_056123.2. NM_015308.2. [Q8N3X1-1]
XP_005252890.1. XM_005252833.1. [Q8N3X1-2]
UniGeneHs.6834.

3D structure databases

ProteinModelPortalQ8N3X1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116941. 12 interactions.
IntActQ8N3X1. 4 interactions.
MINTMINT-127221.
STRING9606.ENSP00000263773.

PTM databases

PhosphoSiteQ8N3X1.

Polymorphism databases

DMDM313104235.

Proteomic databases

MaxQBQ8N3X1.
PaxDbQ8N3X1.
PRIDEQ8N3X1.

Protocols and materials databases

DNASU23360.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000263773; ENSP00000263773; ENSG00000109920. [Q8N3X1-1]
GeneID23360.
KEGGhsa:23360.
UCSCuc001ngj.3. human. [Q8N3X1-1]

Organism-specific databases

CTD23360.
GeneCardsGC11M048805.
H-InvDBHIX0201592.
HGNCHGNC:19752. FNBP4.
HPAHPA039485.
HPA040029.
MIM615265. gene.
neXtProtNX_Q8N3X1.
Orphanet1106. Microphthalmia with limb anomalies.
PharmGKBPA134971679.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG46362.
HOGENOMHOG000168238.
HOVERGENHBG066308.
InParanoidQ8N3X1.
OMAKMPPPEK.
OrthoDBEOG75F4GV.
PhylomeDBQ8N3X1.
TreeFamTF331046.

Enzyme and pathway databases

SignaLinkQ8N3X1.

Gene expression databases

BgeeQ8N3X1.
CleanExHS_FNBP4.
GenevestigatorQ8N3X1.

Family and domain databases

InterProIPR001202. WW_dom.
[Graphical view]
PfamPF00397. WW. 2 hits.
[Graphical view]
SMARTSM00456. WW. 2 hits.
[Graphical view]
SUPFAMSSF51045. SSF51045. 2 hits.
PROSITEPS01159. WW_DOMAIN_1. 1 hit.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSFNBP4. human.
GeneWikiFNBP4.
GenomeRNAi23360.
NextBio45391.
PMAP-CutDBQ8N3X1.
PROQ8N3X1.
SOURCESearch...

Entry information

Entry nameFNBP4_HUMAN
AccessionPrimary (citable) accession number: Q8N3X1
Secondary accession number(s): Q9H985, Q9NT81, Q9Y2L7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 30, 2010
Last modified: July 9, 2014
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM