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Protein

Polypeptide N-acetylgalactosaminyltransferase 15

Gene

GALNT15

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Although it displays a much weaker activity toward all substrates tested compared to GALNT2, it is able to transfer up to seven GalNAc residues to the Muc5AC peptide, suggesting that it can fill vicinal Thr/Ser residues in cooperation with other GALNT proteins. Prefers Muc1a as substrate.

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.1 Publication

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei231SubstrateBy similarity1
Binding sitei260SubstrateBy similarity1
Metal bindingi283ManganeseBy similarity1
Metal bindingi285ManganeseBy similarity1
Metal bindingi417ManganeseBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Lectin, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:HS13381-MONOMER.
ReactomeiR-HSA-913709. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 15 (EC:2.4.1.41)
Alternative name(s):
Polypeptide GalNAc transferase-like protein 2
Short name:
GalNAc-T-like protein 2
Short name:
pp-GaNTase-like protein 2
Polypeptide N-acetylgalactosaminyltransferase-like protein 2
Protein-UDP acetylgalactosaminyltransferase-like protein 2
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 2
Gene namesi
Name:GALNT15
Synonyms:GALNTL2
ORF Names:UNQ770/PRO1564
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:21531. GALNT15.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 34Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
Topological domaini35 – 639LumenalSequence analysisAdd BLAST605

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi117248.
OpenTargetsiENSG00000131386.
PharmGKBiPA134936170.

Polymorphism and mutation databases

BioMutaiGALNT15.
DMDMi51316023.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000591371 – 639Polypeptide N-acetylgalactosaminyltransferase 15Add BLAST639

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi107N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi181 ↔ 412PROSITE-ProRule annotation
Disulfide bondi403 ↔ 482PROSITE-ProRule annotation
Disulfide bondi517 ↔ 536PROSITE-ProRule annotation
Disulfide bondi562 ↔ 575PROSITE-ProRule annotation
Glycosylationi574N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi603 ↔ 620PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8N3T1.
PeptideAtlasiQ8N3T1.
PRIDEiQ8N3T1.

PTM databases

PhosphoSitePlusiQ8N3T1.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in small intestine, placenta, spleen, cerebral cortex and ovary. Expressed at intermediate level in uterus, mammary gland, stomach, cerebellum and whole brain. Weakly expressed in fetal brain, bone marrow, thyroid gland, thymus, heart, skeletal muscle, lung, liver, colon, pancreas, kidney and testis. Not expressed in leukocyte. Expressed in both normal and osteoarthritic cartilage. Expressed at low level in chondrocytes in all zones of both normal and osteoarthritic cartilage.2 Publications

Gene expression databases

BgeeiENSG00000131386.
CleanExiHS_GALNTL2.
ExpressionAtlasiQ8N3T1. baseline and differential.
GenevisibleiQ8N3T1. HS.

Organism-specific databases

HPAiHPA017076.

Interactioni

Protein-protein interaction databases

BioGridi125583. 1 interactor.
IntActiQ8N3T1. 1 interactor.
MINTiMINT-4724072.
STRINGi9606.ENSP00000344260.

Structurei

3D structure databases

ProteinModelPortaliQ8N3T1.
SMRiQ8N3T1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini504 – 631Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST128

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 299Catalytic subdomain AAdd BLAST110
Regioni358 – 420Catalytic subdomain BAdd BLAST63

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

Sequence similaritiesi

Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3736. Eukaryota.
ENOG410XPMK. LUCA.
GeneTreeiENSGT00670000097647.
HOGENOMiHOG000038227.
HOVERGENiHBG051699.
InParanoidiQ8N3T1.
KOiK00710.
OMAiETWLGSF.
OrthoDBiEOG091G04OC.
PhylomeDBiQ8N3T1.
TreeFamiTF313267.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8N3T1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLRKRYRHR PCRLQFLLLL LMLGCVLMMV AMLHPPHHTL HQTVTAQASK
60 70 80 90 100
HSPEARYRLD FGESQDWVLE AEDEGEEYSP LEGLPPFISL REDQLLVAVA
110 120 130 140 150
LPQARRNQSQ GRRGGSYRLI KQPRRQDKEA PKRDWGADED GEVSEEEELT
160 170 180 190 200
PFSLDPRGLQ EALSARIPLQ RALPEVRHPL CLQQHPQDSL PTASVILCFH
210 220 230 240 250
DEAWSTLLRT VHSILDTVPR AFLKEIILVD DLSQQGQLKS ALSEYVARLE
260 270 280 290 300
GVKLLRSNKR LGAIRARMLG ATRATGDVLV FMDAHCECHP GWLEPLLSRI
310 320 330 340 350
AGDRSRVVSP VIDVIDWKTF QYYPSKDLQR GVLDWKLDFH WEPLPEHVRK
360 370 380 390 400
ALQSPISPIR SPVVPGEVVA MDRHYFQNTG AYDSLMSLRG GENLELSFKA
410 420 430 440 450
WLCGGSVEIL PCSRVGHIYQ NQDSHSPLDQ EATLRNRVRI AETWLGSFKE
460 470 480 490 500
TFYKHSPEAF SLSKAEKPDC MERLQLQRRL GCRTFHWFLA NVYPELYPSE
510 520 530 540 550
PRPSFSGKLH NTGLGLCADC QAEGDILGCP MVLAPCSDSR QQQYLQHTSR
560 570 580 590 600
KEIHFGSPQH LCFAVRQEQV ILQNCTEEGL AIHQQHWDFQ ENGMIVHILS
610 620 630
GKCMEAVVQE NNKDLYLRPC DGKARQQWRF DQINAVDER
Length:639
Mass (Da):73,063
Last modified:August 16, 2004 - v2
Checksum:iF5DCA523AF4965DA
GO

Sequence cautioni

The sequence CAD89983 differs from that shown. Reason: Frameshift at positions 169, 192, 206, 222 and 270.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44V → D in CAD38585 (PubMed:17974005).Curated1
Sequence conflicti326K → R in CAD89983 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04924368V → G.Corresponds to variant rs36026882dbSNPEnsembl.1
Natural variantiVAR_049244151P → L.Corresponds to variant rs11715981dbSNPEnsembl.1
Natural variantiVAR_049245324P → A.Corresponds to variant rs12634179dbSNPEnsembl.1
Natural variantiVAR_049246432A → T.2 PublicationsCorresponds to variant rs17851238dbSNPEnsembl.1
Natural variantiVAR_019593510H → Y.Corresponds to variant rs2271077dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB078149 mRNA. Translation: BAD29961.1.
AY035399 mRNA. Translation: AAK63127.1.
AY358443 mRNA. Translation: AAQ88808.1.
AK312425 mRNA. Translation: BAG35335.1.
AL831925 mRNA. Translation: CAD38585.1.
AL832575 mRNA. Translation: CAD89983.1. Frameshift.
AC087858 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW64266.1.
BC014789 mRNA. Translation: AAH14789.1.
CCDSiCCDS33711.1.
RefSeqiNP_473451.3. NM_054110.4.
UniGeneiHs.411308.

Genome annotation databases

EnsembliENST00000339732; ENSP00000344260; ENSG00000131386.
GeneIDi117248.
KEGGihsa:117248.
UCSCiuc003car.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

Polypeptide N-acetylgalactosaminyltransferase-like protein 2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB078149 mRNA. Translation: BAD29961.1.
AY035399 mRNA. Translation: AAK63127.1.
AY358443 mRNA. Translation: AAQ88808.1.
AK312425 mRNA. Translation: BAG35335.1.
AL831925 mRNA. Translation: CAD38585.1.
AL832575 mRNA. Translation: CAD89983.1. Frameshift.
AC087858 Genomic DNA. No translation available.
CH471055 Genomic DNA. Translation: EAW64266.1.
BC014789 mRNA. Translation: AAH14789.1.
CCDSiCCDS33711.1.
RefSeqiNP_473451.3. NM_054110.4.
UniGeneiHs.411308.

3D structure databases

ProteinModelPortaliQ8N3T1.
SMRiQ8N3T1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125583. 1 interactor.
IntActiQ8N3T1. 1 interactor.
MINTiMINT-4724072.
STRINGi9606.ENSP00000344260.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

PTM databases

PhosphoSitePlusiQ8N3T1.

Polymorphism and mutation databases

BioMutaiGALNT15.
DMDMi51316023.

Proteomic databases

PaxDbiQ8N3T1.
PeptideAtlasiQ8N3T1.
PRIDEiQ8N3T1.

Protocols and materials databases

DNASUi117248.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000339732; ENSP00000344260; ENSG00000131386.
GeneIDi117248.
KEGGihsa:117248.
UCSCiuc003car.5. human.

Organism-specific databases

CTDi117248.
DisGeNETi117248.
GeneCardsiGALNT15.
HGNCiHGNC:21531. GALNT15.
HPAiHPA017076.
MIMi615131. gene.
neXtProtiNX_Q8N3T1.
OpenTargetsiENSG00000131386.
PharmGKBiPA134936170.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3736. Eukaryota.
ENOG410XPMK. LUCA.
GeneTreeiENSGT00670000097647.
HOGENOMiHOG000038227.
HOVERGENiHBG051699.
InParanoidiQ8N3T1.
KOiK00710.
OMAiETWLGSF.
OrthoDBiEOG091G04OC.
PhylomeDBiQ8N3T1.
TreeFamiTF313267.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciZFISH:HS13381-MONOMER.
ReactomeiR-HSA-913709. O-linked glycosylation of mucins.

Miscellaneous databases

GeneWikiiGALNTL2.
GenomeRNAii117248.
PROiQ8N3T1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000131386.
CleanExiHS_GALNTL2.
ExpressionAtlasiQ8N3T1. baseline and differential.
GenevisibleiQ8N3T1. HS.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLT15_HUMAN
AccessioniPrimary (citable) accession number: Q8N3T1
Secondary accession number(s): A6NMN1
, B2R638, F1LIP6, Q86T60, Q96C46, Q96DJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: November 2, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was originally termed Galnt15/pp-GaNTase 15.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.