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Protein

Polypeptide N-acetylgalactosaminyltransferase 15

Gene

GALNT15

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Although it displays a much weaker activity toward all substrates tested compared to GALNT2, it is able to transfer up to seven GalNAc residues to the Muc5AC peptide, suggesting that it can fill vicinal Thr/Ser residues in cooperation with other GALNT proteins. Prefers Muc1a as substrate.

Caution

Was originally termed Galnt15/pp-GaNTase 15.1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.1 Publication

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei231SubstrateBy similarity1
Binding sitei260SubstrateBy similarity1
Metal bindingi283ManganeseBy similarity1
Metal bindingi285ManganeseBy similarity1
Metal bindingi417ManganeseBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandLectin, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-913709 O-linked glycosylation of mucins
UniPathwayiUPA00378

Protein family/group databases

CAZyiCBM13 Carbohydrate-Binding Module Family 13
GT27 Glycosyltransferase Family 27

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 15 (EC:2.4.1.41)
Alternative name(s):
Polypeptide GalNAc transferase-like protein 2
Short name:
GalNAc-T-like protein 2
Short name:
pp-GaNTase-like protein 2
Polypeptide N-acetylgalactosaminyltransferase-like protein 2
Protein-UDP acetylgalactosaminyltransferase-like protein 2
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 2
Gene namesi
Name:GALNT15
Synonyms:GALNTL2
ORF Names:UNQ770/PRO1564
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000131386.17
HGNCiHGNC:21531 GALNT15
MIMi615131 gene
neXtProtiNX_Q8N3T1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 34Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
Topological domaini35 – 639LumenalSequence analysisAdd BLAST605

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi117248
OpenTargetsiENSG00000131386
PharmGKBiPA134936170

Polymorphism and mutation databases

BioMutaiGALNT15
DMDMi51316023

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000591371 – 639Polypeptide N-acetylgalactosaminyltransferase 15Add BLAST639

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi107N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi181 ↔ 412PROSITE-ProRule annotation
Disulfide bondi403 ↔ 482PROSITE-ProRule annotation
Disulfide bondi517 ↔ 536PROSITE-ProRule annotation
Disulfide bondi562 ↔ 575PROSITE-ProRule annotation
Glycosylationi574N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi603 ↔ 620PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8N3T1
PeptideAtlasiQ8N3T1
PRIDEiQ8N3T1

PTM databases

PhosphoSitePlusiQ8N3T1

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in small intestine, placenta, spleen, cerebral cortex and ovary. Expressed at intermediate level in uterus, mammary gland, stomach, cerebellum and whole brain. Weakly expressed in fetal brain, bone marrow, thyroid gland, thymus, heart, skeletal muscle, lung, liver, colon, pancreas, kidney and testis. Not expressed in leukocyte. Expressed in both normal and osteoarthritic cartilage. Expressed at low level in chondrocytes in all zones of both normal and osteoarthritic cartilage.2 Publications

Gene expression databases

BgeeiENSG00000131386
CleanExiHS_GALNTL2
ExpressionAtlasiQ8N3T1 baseline and differential
GenevisibleiQ8N3T1 HS

Organism-specific databases

HPAiHPA017076
HPA051551

Interactioni

Protein-protein interaction databases

BioGridi125583, 1 interactor
IntActiQ8N3T1, 2 interactors
STRINGi9606.ENSP00000344260

Structurei

3D structure databases

ProteinModelPortaliQ8N3T1
SMRiQ8N3T1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini504 – 631Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST128

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 299Catalytic subdomain AAdd BLAST110
Regioni358 – 420Catalytic subdomain BAdd BLAST63

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3736 Eukaryota
ENOG410XPMK LUCA
GeneTreeiENSGT00900000140827
HOGENOMiHOG000038227
HOVERGENiHBG051699
InParanoidiQ8N3T1
KOiK00710
OMAiMLAPCSD
OrthoDBiEOG091G04OC
PhylomeDBiQ8N3T1
TreeFamiTF313267

Family and domain databases

CDDicd00161 RICIN, 1 hit
Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR001173 Glyco_trans_2-like
IPR029044 Nucleotide-diphossugar_trans
IPR035992 Ricin_B-like_lectins
IPR000772 Ricin_B_lectin
PfamiView protein in Pfam
PF00535 Glycos_transf_2, 1 hit
PF00652 Ricin_B_lectin, 1 hit
SMARTiView protein in SMART
SM00458 RICIN, 1 hit
SUPFAMiSSF50370 SSF50370, 1 hit
SSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS50231 RICIN_B_LECTIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q8N3T1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLRKRYRHR PCRLQFLLLL LMLGCVLMMV AMLHPPHHTL HQTVTAQASK
60 70 80 90 100
HSPEARYRLD FGESQDWVLE AEDEGEEYSP LEGLPPFISL REDQLLVAVA
110 120 130 140 150
LPQARRNQSQ GRRGGSYRLI KQPRRQDKEA PKRDWGADED GEVSEEEELT
160 170 180 190 200
PFSLDPRGLQ EALSARIPLQ RALPEVRHPL CLQQHPQDSL PTASVILCFH
210 220 230 240 250
DEAWSTLLRT VHSILDTVPR AFLKEIILVD DLSQQGQLKS ALSEYVARLE
260 270 280 290 300
GVKLLRSNKR LGAIRARMLG ATRATGDVLV FMDAHCECHP GWLEPLLSRI
310 320 330 340 350
AGDRSRVVSP VIDVIDWKTF QYYPSKDLQR GVLDWKLDFH WEPLPEHVRK
360 370 380 390 400
ALQSPISPIR SPVVPGEVVA MDRHYFQNTG AYDSLMSLRG GENLELSFKA
410 420 430 440 450
WLCGGSVEIL PCSRVGHIYQ NQDSHSPLDQ EATLRNRVRI AETWLGSFKE
460 470 480 490 500
TFYKHSPEAF SLSKAEKPDC MERLQLQRRL GCRTFHWFLA NVYPELYPSE
510 520 530 540 550
PRPSFSGKLH NTGLGLCADC QAEGDILGCP MVLAPCSDSR QQQYLQHTSR
560 570 580 590 600
KEIHFGSPQH LCFAVRQEQV ILQNCTEEGL AIHQQHWDFQ ENGMIVHILS
610 620 630
GKCMEAVVQE NNKDLYLRPC DGKARQQWRF DQINAVDER
Length:639
Mass (Da):73,063
Last modified:August 16, 2004 - v2
Checksum:iF5DCA523AF4965DA
GO

Sequence cautioni

The sequence CAD89983 differs from that shown. Reason: Frameshift at positions 169, 192, 206, 222 and 270.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44V → D in CAD38585 (PubMed:17974005).Curated1
Sequence conflicti326K → R in CAD89983 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04924368V → G. Corresponds to variant dbSNP:rs36026882Ensembl.1
Natural variantiVAR_049244151P → L. Corresponds to variant dbSNP:rs11715981Ensembl.1
Natural variantiVAR_049245324P → A. Corresponds to variant dbSNP:rs12634179Ensembl.1
Natural variantiVAR_049246432A → T2 PublicationsCorresponds to variant dbSNP:rs17851238Ensembl.1
Natural variantiVAR_019593510H → Y. Corresponds to variant dbSNP:rs2271077Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB078149 mRNA Translation: BAD29961.1
AY035399 mRNA Translation: AAK63127.1
AY358443 mRNA Translation: AAQ88808.1
AK312425 mRNA Translation: BAG35335.1
AL831925 mRNA Translation: CAD38585.1
AL832575 mRNA Translation: CAD89983.1 Frameshift.
AC087858 Genomic DNA No translation available.
CH471055 Genomic DNA Translation: EAW64266.1
BC014789 mRNA Translation: AAH14789.1
CCDSiCCDS33711.1
RefSeqiNP_473451.3, NM_054110.4
UniGeneiHs.411308

Genome annotation databases

EnsembliENST00000339732; ENSP00000344260; ENSG00000131386
GeneIDi117248
KEGGihsa:117248
UCSCiuc003car.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiGLT15_HUMAN
AccessioniPrimary (citable) accession number: Q8N3T1
Secondary accession number(s): A6NMN1
, B2R638, F1LIP6, Q86T60, Q96C46, Q96DJ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: March 28, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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