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Protein

MAGUK p55 subfamily member 5

Gene

MPP5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton (By similarity).By similarity

GO - Molecular functioni

  • protein domain specific binding Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000072415-MONOMER.
ReactomeiR-HSA-420029. Tight junction interactions.
SIGNORiQ8N3R9.

Names & Taxonomyi

Protein namesi
Recommended name:
MAGUK p55 subfamily member 5
Gene namesi
Name:MPP5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:18669. MPP5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

Pathology & Biotechi

Organism-specific databases

DisGeNETi64398.
OpenTargetsiENSG00000072415.
PharmGKBiPA38631.

Polymorphism and mutation databases

BioMutaiMPP5.
DMDMi116242632.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000945801 – 675MAGUK p55 subfamily member 5Add BLAST675

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineCombined sources1
Modified residuei25PhosphoserineCombined sources1
Modified residuei83PhosphoserineCombined sources1
Modified residuei84PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8N3R9.
MaxQBiQ8N3R9.
PaxDbiQ8N3R9.
PeptideAtlasiQ8N3R9.
PRIDEiQ8N3R9.

PTM databases

iPTMnetiQ8N3R9.
PhosphoSitePlusiQ8N3R9.
SwissPalmiQ8N3R9.

Expressioni

Tissue specificityi

Expressed in the retina (at protein level).2 Publications

Gene expression databases

BgeeiENSG00000072415.
CleanExiHS_MPP5.
ExpressionAtlasiQ8N3R9. baseline and differential.
GenevisibleiQ8N3R9. HS.

Organism-specific databases

HPAiHPA000993.

Interactioni

Subunit structurei

Interacts with CRB3, LIN7C and MPDZ. Interacts with PATJ, PARD6B, SC6A1 and EZR (By similarity). Forms a complex with CRB1 and MPP4. Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, PATJ and PARD3/PAR3. Heterodimer with MPP1. Interacts with MPP7.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
INADLQ8NI354EBI-2513978,EBI-724390
LIN7AO149107EBI-2513978,EBI-2513988

GO - Molecular functioni

  • protein domain specific binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi122155. 29 interactors.
IntActiQ8N3R9. 25 interactors.
MINTiMINT-1772982.
STRINGi9606.ENSP00000261681.

Structurei

Secondary structure

1675
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi124 – 137Combined sources14
Helixi141 – 155Combined sources15
Helixi157 – 173Combined sources17
Helixi186 – 197Combined sources12
Turni198 – 200Combined sources3
Helixi203 – 213Combined sources11
Helixi215 – 229Combined sources15
Beta strandi230 – 232Combined sources3
Beta strandi249 – 251Combined sources3
Beta strandi255 – 261Combined sources7
Beta strandi268 – 274Combined sources7
Beta strandi277 – 283Combined sources7
Helixi288 – 292Combined sources5
Beta strandi300 – 304Combined sources5
Helixi314 – 322Combined sources9
Beta strandi326 – 333Combined sources8
Beta strandi348 – 352Combined sources5
Helixi358 – 360Combined sources3
Helixi367 – 369Combined sources3
Beta strandi378 – 383Combined sources6
Beta strandi389 – 394Combined sources6
Beta strandi405 – 408Combined sources4
Beta strandi464 – 472Combined sources9
Beta strandi482 – 486Combined sources5
Turni488 – 491Combined sources4
Helixi492 – 502Combined sources11
Turni503 – 506Combined sources4
Turni524 – 527Combined sources4
Helixi533 – 541Combined sources9
Beta strandi545 – 551Combined sources7
Beta strandi554 – 559Combined sources6
Helixi560 – 567Combined sources8
Turni568 – 570Combined sources3
Beta strandi571 – 576Combined sources6
Helixi579 – 581Combined sources3
Helixi582 – 586Combined sources5
Beta strandi593 – 598Combined sources6
Helixi602 – 608Combined sources7
Helixi621 – 631Combined sources11
Helixi634 – 636Combined sources3
Beta strandi639 – 644Combined sources6
Helixi648 – 660Combined sources13
Turni661 – 664Combined sources4
Beta strandi667 – 670Combined sources4
Helixi671 – 673Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y76NMR-B/D118-177[»]
3UITX-ray2.05A/B/C/D119-232[»]
4UU5X-ray1.23A251-335[»]
4UU6X-ray1.80A251-335[»]
4WSIX-ray2.95A/B236-675[»]
ProteinModelPortaliQ8N3R9.
SMRiQ8N3R9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8N3R9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini120 – 177L27 1PROSITE-ProRule annotationAdd BLAST58
Domaini179 – 235L27 2PROSITE-ProRule annotationAdd BLAST57
Domaini256 – 336PDZPROSITE-ProRule annotationAdd BLAST81
Domaini345 – 417SH3PROSITE-ProRule annotationAdd BLAST73
Domaini479 – 660Guanylate kinase-likePROSITE-ProRule annotationAdd BLAST182

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 140Interaction with PARD6BBy similarityAdd BLAST120
Regioni181 – 243Interaction with LIN7CBy similarityAdd BLAST63

Domaini

The L27 domain 1 functions in targeting to the tight junctions by binding to PATJ.By similarity
The PDZ domain binds to the C-terminus of SC6A1.By similarity

Sequence similaritiesi

Belongs to the MAGUK family.Curated
Contains 1 guanylate kinase-like domain.PROSITE-ProRule annotation
Contains 2 L27 domains.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG0609. Eukaryota.
COG0194. LUCA.
GeneTreeiENSGT00760000118866.
HOGENOMiHOG000233034.
HOVERGENiHBG001858.
InParanoidiQ8N3R9.
KOiK06091.
OMAiHQKEGQE.
OrthoDBiEOG091G065I.
PhylomeDBiQ8N3R9.
TreeFamiTF314263.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR020590. Guanylate_kinase_CS.
IPR014775. L27_C.
IPR004172. L27_dom.
IPR015145. L27_N.
IPR031185. MPP5.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23122:SF14. PTHR23122:SF14. 1 hit.
PfamiPF00625. Guanylate_kin. 1 hit.
PF02828. L27. 1 hit.
PF09060. L27_N. 1 hit.
PF00595. PDZ. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
SM00569. L27. 2 hits.
SM00228. PDZ. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF101288. SSF101288. 2 hits.
SSF50044. SSF50044. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS51022. L27. 2 hits.
PS50106. PDZ. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N3R9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTSHMNGHV TEESDSEVKN VDLASPEEHQ KHREMAVDCP GDLGTRMMPI
60 70 80 90 100
RRSAQLERIR QQQEDMRRRR EEEGKKQELD LNSSMRLKKL AQIPPKTGID
110 120 130 140 150
NPMFDTEEGI VLESPHYAVK ILEIEDLFSS LKHIQHTLVD SQSQEDISLL
160 170 180 190 200
LQLVQNKDFQ NAFKIHNAIT VHMNKASPPF PLISNAQDLA QEVQTVLKPV
210 220 230 240 250
HHKEGQELTA LLNTPHIQAL LLAHDKVAEQ EMQLEPITDE RVYESIGQYG
260 270 280 290 300
GETVKIVRIE KARDIPLGAT VRNEMDSVII SRIVKGGAAE KSGLLHEGDE
310 320 330 340 350
VLEINGIEIR GKDVNEVFDL LSDMHGTLTF VLIPSQQIKP PPAKETVIHV
360 370 380 390 400
KAHFDYDPSD DPYVPCRELG LSFQKGDILH VISQEDPNWW QAYREGDEDN
410 420 430 440 450
QPLAGLVPGK SFQQQREAMK QTIEEDKEPE KSGKLWCAKK NKKKRKKVLY
460 470 480 490 500
NANKNDDYDN EEILTYEEMS LYHQPANRKR PIILIGPQNC GQNELRQRLM
510 520 530 540 550
NKEKDRFASA VPHTTRSRRD QEVAGRDYHF VSRQAFEADI AAGKFIEHGE
560 570 580 590 600
FEKNLYGTSI DSVRQVINSG KICLLSLRTQ SLKTLRNSDL KPYIIFIAPP
610 620 630 640 650
SQERLRALLA KEGKNPKPEE LREIIEKTRE MEQNNGHYFD TAIVNSDLDK
660 670
AYQELLRLIN KLDTEPQWVP STWLR
Length:675
Mass (Da):77,294
Last modified:October 17, 2006 - v3
Checksum:iA3B2CB594E0908CA
GO
Isoform 2 (identifier: Q8N3R9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: Missing.

Show »
Length:641
Mass (Da):73,421
Checksum:iAD604D26B3580FDB
GO

Sequence cautioni

The sequence AAH53366 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAB14172 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti100D → G in CAD38620 (PubMed:17974005).Curated1
Sequence conflicti141S → Y in CAD89937 (PubMed:17974005).Curated1
Sequence conflicti161N → S in BAC05295 (PubMed:14702039).Curated1
Sequence conflicti324M → L in BAC05295 (PubMed:14702039).Curated1
Sequence conflicti401Q → H in CAD89937 (PubMed:17974005).Curated1
Sequence conflicti460N → H in CAD89937 (PubMed:17974005).Curated1
Sequence conflicti490C → F in BAB14172 (PubMed:14702039).Curated1
Sequence conflicti613G → V in CAD38620 (PubMed:17974005).Curated1
Sequence conflicti634N → S in CAD38620 (PubMed:17974005).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0140021 – 34Missing in isoform 2. 1 PublicationAdd BLAST34

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL832326 mRNA. Translation: CAD38620.1.
AL832578 mRNA. Translation: CAD89937.1.
CH471061 Genomic DNA. Translation: EAW80930.1.
BC053366 mRNA. Translation: AAH53366.1. Sequence problems.
BC129933 mRNA. Translation: AAI29934.1.
AK022677 mRNA. Translation: BAB14172.1. Different initiation.
AK098373 mRNA. Translation: BAC05295.1.
CCDSiCCDS58325.1. [Q8N3R9-2]
CCDS9779.1. [Q8N3R9-1]
RefSeqiNP_001243479.1. NM_001256550.1. [Q8N3R9-2]
NP_071919.2. NM_022474.3. [Q8N3R9-1]
XP_005268060.1. XM_005268003.1. [Q8N3R9-2]
XP_011535388.1. XM_011537086.2. [Q8N3R9-1]
XP_011535389.1. XM_011537087.2. [Q8N3R9-1]
UniGeneiHs.652312.
Hs.740524.

Genome annotation databases

EnsembliENST00000261681; ENSP00000261681; ENSG00000072415. [Q8N3R9-1]
ENST00000555925; ENSP00000451488; ENSG00000072415. [Q8N3R9-2]
GeneIDi64398.
KEGGihsa:64398.
UCSCiuc001xjc.5. human. [Q8N3R9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL832326 mRNA. Translation: CAD38620.1.
AL832578 mRNA. Translation: CAD89937.1.
CH471061 Genomic DNA. Translation: EAW80930.1.
BC053366 mRNA. Translation: AAH53366.1. Sequence problems.
BC129933 mRNA. Translation: AAI29934.1.
AK022677 mRNA. Translation: BAB14172.1. Different initiation.
AK098373 mRNA. Translation: BAC05295.1.
CCDSiCCDS58325.1. [Q8N3R9-2]
CCDS9779.1. [Q8N3R9-1]
RefSeqiNP_001243479.1. NM_001256550.1. [Q8N3R9-2]
NP_071919.2. NM_022474.3. [Q8N3R9-1]
XP_005268060.1. XM_005268003.1. [Q8N3R9-2]
XP_011535388.1. XM_011537086.2. [Q8N3R9-1]
XP_011535389.1. XM_011537087.2. [Q8N3R9-1]
UniGeneiHs.652312.
Hs.740524.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y76NMR-B/D118-177[»]
3UITX-ray2.05A/B/C/D119-232[»]
4UU5X-ray1.23A251-335[»]
4UU6X-ray1.80A251-335[»]
4WSIX-ray2.95A/B236-675[»]
ProteinModelPortaliQ8N3R9.
SMRiQ8N3R9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122155. 29 interactors.
IntActiQ8N3R9. 25 interactors.
MINTiMINT-1772982.
STRINGi9606.ENSP00000261681.

PTM databases

iPTMnetiQ8N3R9.
PhosphoSitePlusiQ8N3R9.
SwissPalmiQ8N3R9.

Polymorphism and mutation databases

BioMutaiMPP5.
DMDMi116242632.

Proteomic databases

EPDiQ8N3R9.
MaxQBiQ8N3R9.
PaxDbiQ8N3R9.
PeptideAtlasiQ8N3R9.
PRIDEiQ8N3R9.

Protocols and materials databases

DNASUi64398.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261681; ENSP00000261681; ENSG00000072415. [Q8N3R9-1]
ENST00000555925; ENSP00000451488; ENSG00000072415. [Q8N3R9-2]
GeneIDi64398.
KEGGihsa:64398.
UCSCiuc001xjc.5. human. [Q8N3R9-1]

Organism-specific databases

CTDi64398.
DisGeNETi64398.
GeneCardsiMPP5.
HGNCiHGNC:18669. MPP5.
HPAiHPA000993.
MIMi606958. gene.
neXtProtiNX_Q8N3R9.
OpenTargetsiENSG00000072415.
PharmGKBiPA38631.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0609. Eukaryota.
COG0194. LUCA.
GeneTreeiENSGT00760000118866.
HOGENOMiHOG000233034.
HOVERGENiHBG001858.
InParanoidiQ8N3R9.
KOiK06091.
OMAiHQKEGQE.
OrthoDBiEOG091G065I.
PhylomeDBiQ8N3R9.
TreeFamiTF314263.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000072415-MONOMER.
ReactomeiR-HSA-420029. Tight junction interactions.
SIGNORiQ8N3R9.

Miscellaneous databases

ChiTaRSiMPP5. human.
EvolutionaryTraceiQ8N3R9.
GeneWikiiMPP5.
GenomeRNAii64398.
PROiQ8N3R9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000072415.
CleanExiHS_MPP5.
ExpressionAtlasiQ8N3R9. baseline and differential.
GenevisibleiQ8N3R9. HS.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR020590. Guanylate_kinase_CS.
IPR014775. L27_C.
IPR004172. L27_dom.
IPR015145. L27_N.
IPR031185. MPP5.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23122:SF14. PTHR23122:SF14. 1 hit.
PfamiPF00625. Guanylate_kin. 1 hit.
PF02828. L27. 1 hit.
PF09060. L27_N. 1 hit.
PF00595. PDZ. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
SM00569. L27. 2 hits.
SM00228. PDZ. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF101288. SSF101288. 2 hits.
SSF50044. SSF50044. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
PS51022. L27. 2 hits.
PS50106. PDZ. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPP5_HUMAN
AccessioniPrimary (citable) accession number: Q8N3R9
Secondary accession number(s): A1L380
, Q7Z631, Q86T98, Q8N7I5, Q9H9Q0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: October 17, 2006
Last modified: November 30, 2016
This is version 157 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.