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Protein

Vacuolar protein sorting-associated protein 8 homolog

Gene

VPS8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in vesicle-mediated protein trafficking of the endocytic membrane transport pathway. Believed to act as a component of the putative CORVET endosomal tethering complexes which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed:25266290). Functions predominantly in APPL1-containing endosomes (PubMed:25266290).1 Publication1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1258 – 131053RING-type; atypicalPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • endosomal vesicle fusion Source: UniProtKB
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 8 homolog
Gene namesi
Name:VPS8
Synonyms:KIAA0804
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:29122. VPS8.

Subcellular locationi

GO - Cellular componenti

  • CORVET complex Source: Ensembl
  • early endosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endosome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671617.
PA142671888.

Polymorphism and mutation databases

BioMutaiVPS8.
DMDMi296452997.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14281428Vacuolar protein sorting-associated protein 8 homologPRO_0000278267Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei26 – 261PhosphoserineCombined sources
Modified residuei32 – 321PhosphoserineCombined sources
Modified residuei127 – 1271PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8N3P4.
MaxQBiQ8N3P4.
PaxDbiQ8N3P4.
PeptideAtlasiQ8N3P4.
PRIDEiQ8N3P4.

PTM databases

iPTMnetiQ8N3P4.
PhosphoSiteiQ8N3P4.

Expressioni

Gene expression databases

BgeeiENSG00000156931.
CleanExiHS_VPS8.
ExpressionAtlasiQ8N3P4. baseline and differential.
GenevisibleiQ8N3P4. HS.

Organism-specific databases

HPAiHPA036871.

Interactioni

Subunit structurei

Interacts with RAB5C (By similarity). Interacts with TGFBRAP1 (PubMed:25266290). Component of the putative class C core vacuaole/endosome tethering (CORVET) complex; the core of which composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, is associated with VPS8 and TGFBRAP1 (PubMed:25266290).By similarity1 Publication1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TGFBRAP1Q8WUH22EBI-7261494,EBI-2954829

Protein-protein interaction databases

BioGridi116937. 17 interactions.
IntActiQ8N3P4. 8 interactions.
MINTiMINT-1196904.
STRINGi9606.ENSP00000397879.

Structurei

3D structure databases

ProteinModelPortaliQ8N3P4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati195 – 23642WDAdd
BLAST

Sequence similaritiesi

Belongs to the VPS8 family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 1 WD repeat.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1258 – 131053RING-type; atypicalPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

WD repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2079. Eukaryota.
ENOG410XRX6. LUCA.
GeneTreeiENSGT00390000010672.
HOGENOMiHOG000013171.
HOVERGENiHBG071091.
InParanoidiQ8N3P4.
KOiK20178.
PhylomeDBiQ8N3P4.
TreeFamiTF314244.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR011044. Quino_amine_DH_bsu.
IPR025941. Vps8_central_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF12816. Vps8. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF50969. SSF50969. 2 hits.
SSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N3P4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENEPDHENV EQSLCAKTSE EELNKSFNLE ASLSKFSYID MDKELEFKND
60 70 80 90 100
LIDDKEFDIP QVDTPPTLES ILNETDDEDE SFILEDPTLL NIDTIDSHSY
110 120 130 140 150
DTSSVASSDS GDRTNLKRKK KLPDSFSLHG SVMRHSLLKG ISAQIVSAAD
160 170 180 190 200
KVDAGLPTAI AVSSLIAVGT SHGLALIFGK DQNQALRLCL GSTSVGGQYG
210 220 230 240 250
AISALSINND CSRLLCGFAK GQITMWDLAS GKLLRSITDA HPPGTAILHI
260 270 280 290 300
KFTDDPTLAI CNDSGGSVFE LTFKRVMGVR TCESRCLFSG SKGEVCCIEP
310 320 330 340 350
LHSKPELKDH PITQFSLLAM ASLTKILVIG LKPSLKVWMT FPYGRMDPSS
360 370 380 390 400
VPLLAWHFVA VQNYVNPMLA FCRGDVVHFL LVKRDESGAI HVTKQKHLHL
410 420 430 440 450
YYDLINFTWI NSRTVVLLDS VEKLHVIDRQ TQEELETVEI SEVQLVYNSS
460 470 480 490 500
HFKSLATGGN VSQALALVGE KACYQSISSY GGQIFYLGTK SVYVMMLRSW
510 520 530 540 550
RERVDHLLKQ DCLTEALALA WSFHEGKAKA VVGLSGDASK RKAIVADRMV
560 570 580 590 600
EILFHYADRA LKKCPDQGKI QVMEQHFQDM VPVIVDYCLL LQRKDLLFSQ
610 620 630 640 650
MYDKLSENSV AKGVFLECLE PYILSDKLVG ITPQVMKDLI VHFQDKKLME
660 670 680 690 700
NVEALIVHMD ITSLDIQQVV LMCWENRLYD AMIYVYNRGM NEFISPMEKL
710 720 730 740 750
FRVIAPPLNA GKTLTDEQVV MGNKLLVYIS CCLAGRAYPL GDIPEDLVPL
760 770 780 790 800
VKNQVFEFLI RLHSAEASPE EEIYPYIRTL LHFDTREFLN VLALTFEDFK
810 820 830 840 850
NDKQAVEYQQ RIVDILLKVM VENSDFTPSQ VGCLFTFLAR QLAKPDNTLF
860 870 880 890 900
VNRTLFDQVL EFLCSPDDDS RHSERQQVLL ELLQAGGIVQ FEESRLIRMA
910 920 930 940 950
EKAEFYQICE FMYEREHQYD KIIDCYLRDP LREEEVFNYI HNILSIPGHS
960 970 980 990 1000
AEEKQSVWQK AMDHIEELVS LKPCKAAELV ATHFSGHIET VIKKLQNQVL
1010 1020 1030 1040 1050
LFKFLRSLLD PREGIHVNQE LLQISPCITE QFIELLCQFN PTQVIETLQV
1060 1070 1080 1090 1100
LECYRLEETI QITQKYQLHE VTAYLLEKKG DIHGAFLIML ERLQSKLQEV
1110 1120 1130 1140 1150
THQGENTKED PSLKDVEDTM VETIALCQRN SHNLNQQQRE ALWFPLLEAM
1160 1170 1180 1190 1200
MAPQKLSSSA IPHLHSEALK SLTMQVLNSM AAFIALPSIL QRILQDPVYG
1210 1220 1230 1240 1250
KGKLGEIQGL ILGMLDTFNY EQTLLETTTS LLNQDLHWSL CNLRASVTRG
1260 1270 1280 1290 1300
LNPKQDYCSI CLQQYKRRQE MADEIIVFSC GHLYHSFCLQ NKECTVEFEG
1310 1320 1330 1340 1350
QTRWTCYKCS SSNKVGKLSE NSSEIKKGRI TPSQVKMSPS YHQSKGDPTA
1360 1370 1380 1390 1400
KKGTSEPVLD PQQIQAFDQL CRLYRGSSRL ALLTELSQNR SSESYRPFSG
1410 1420
SQSAPAFNSI FQNENFQLQL IPPPVTED
Length:1,428
Mass (Da):161,754
Last modified:May 18, 2010 - v3
Checksum:i97FB5E8D453BBCB6
GO
Isoform 2 (identifier: Q8N3P4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     179-180: Missing.
     578-667: Missing.

Show »
Length:1,336
Mass (Da):151,216
Checksum:i67DBD2AE6D4FBEE6
GO
Isoform 3 (identifier: Q8N3P4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     179-180: Missing.

Note: No experimental confirmation available.
Show »
Length:1,426
Mass (Da):161,568
Checksum:iECF6DAC303CC7B16
GO

Sequence cautioni

The sequence BAB14322 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAH56195 differs from that shown. Reason: Frameshift at position 325. Curated
Isoform 2 : The sequence CAH56195 differs from that shown. Reason: Frameshift at position 326. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti433 – 4331E → G in CAH56195 (PubMed:17974005).Curated
Sequence conflicti471 – 4711K → N in CAH56195 (PubMed:17974005).Curated
Sequence conflicti765 – 7651A → S in BAB14322 (PubMed:14702039).Curated
Sequence conflicti932 – 9321Missing in CAH56195 (PubMed:17974005).Curated
Sequence conflicti1042 – 10421T → A in BAB14322 (PubMed:14702039).Curated
Sequence conflicti1043 – 10431Q → L in CAH56195 (PubMed:17974005).Curated
Sequence conflicti1167 – 11671E → G in BAB14322 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti83 – 831I → V.1 Publication
Corresponds to variant rs9830734 [ dbSNP | Ensembl ].
VAR_030730
Natural varianti1165 – 11651H → Y.1 Publication
Corresponds to variant rs11555405 [ dbSNP | Ensembl ].
VAR_030731
Natural varianti1364 – 13641I → T.
Corresponds to variant rs3821750 [ dbSNP | Ensembl ].
VAR_030732
Natural varianti1372 – 13721R → H.
Corresponds to variant rs16859527 [ dbSNP | Ensembl ].
VAR_030733

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei179 – 1802Missing in isoform 2 and isoform 3. 2 PublicationsVSP_023249
Alternative sequencei578 – 66790Missing in isoform 2. 1 PublicationVSP_023250Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK022945 mRNA. Translation: BAB14322.1. Different initiation.
AK292423 mRNA. Translation: BAF85112.1.
AL833838 mRNA. Translation: CAD38698.1.
BX647915 mRNA. Translation: CAH56195.1. Frameshift.
AC025573 Genomic DNA. No translation available.
AC107294 Genomic DNA. No translation available.
AC117436 Genomic DNA. No translation available.
BC001001 mRNA. Translation: AAH01001.2.
BC140768 mRNA. Translation: AAI40769.1.
AB018347 mRNA. Translation: BAA34524.1.
CCDSiCCDS46971.1. [Q8N3P4-1]
CCDS46972.1. [Q8N3P4-3]
RefSeqiNP_001009921.1. NM_001009921.2. [Q8N3P4-1]
NP_056118.2. NM_015303.3. [Q8N3P4-3]
XP_005247308.1. XM_005247251.3. [Q8N3P4-1]
UniGeneiHs.269263.

Genome annotation databases

EnsembliENST00000436792; ENSP00000404704; ENSG00000156931. [Q8N3P4-3]
ENST00000446204; ENSP00000405483; ENSG00000156931. [Q8N3P4-2]
ENST00000625842; ENSP00000487164; ENSG00000156931. [Q8N3P4-1]
GeneIDi23355.
KEGGihsa:23355.
UCSCiuc003fpb.2. human. [Q8N3P4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK022945 mRNA. Translation: BAB14322.1. Different initiation.
AK292423 mRNA. Translation: BAF85112.1.
AL833838 mRNA. Translation: CAD38698.1.
BX647915 mRNA. Translation: CAH56195.1. Frameshift.
AC025573 Genomic DNA. No translation available.
AC107294 Genomic DNA. No translation available.
AC117436 Genomic DNA. No translation available.
BC001001 mRNA. Translation: AAH01001.2.
BC140768 mRNA. Translation: AAI40769.1.
AB018347 mRNA. Translation: BAA34524.1.
CCDSiCCDS46971.1. [Q8N3P4-1]
CCDS46972.1. [Q8N3P4-3]
RefSeqiNP_001009921.1. NM_001009921.2. [Q8N3P4-1]
NP_056118.2. NM_015303.3. [Q8N3P4-3]
XP_005247308.1. XM_005247251.3. [Q8N3P4-1]
UniGeneiHs.269263.

3D structure databases

ProteinModelPortaliQ8N3P4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116937. 17 interactions.
IntActiQ8N3P4. 8 interactions.
MINTiMINT-1196904.
STRINGi9606.ENSP00000397879.

PTM databases

iPTMnetiQ8N3P4.
PhosphoSiteiQ8N3P4.

Polymorphism and mutation databases

BioMutaiVPS8.
DMDMi296452997.

Proteomic databases

EPDiQ8N3P4.
MaxQBiQ8N3P4.
PaxDbiQ8N3P4.
PeptideAtlasiQ8N3P4.
PRIDEiQ8N3P4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000436792; ENSP00000404704; ENSG00000156931. [Q8N3P4-3]
ENST00000446204; ENSP00000405483; ENSG00000156931. [Q8N3P4-2]
ENST00000625842; ENSP00000487164; ENSG00000156931. [Q8N3P4-1]
GeneIDi23355.
KEGGihsa:23355.
UCSCiuc003fpb.2. human. [Q8N3P4-1]

Organism-specific databases

CTDi23355.
GeneCardsiVPS8.
HGNCiHGNC:29122. VPS8.
HPAiHPA036871.
neXtProtiNX_Q8N3P4.
PharmGKBiPA142671617.
PA142671888.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2079. Eukaryota.
ENOG410XRX6. LUCA.
GeneTreeiENSGT00390000010672.
HOGENOMiHOG000013171.
HOVERGENiHBG071091.
InParanoidiQ8N3P4.
KOiK20178.
PhylomeDBiQ8N3P4.
TreeFamiTF314244.

Miscellaneous databases

ChiTaRSiVPS8. human.
GenomeRNAii23355.
PROiQ8N3P4.

Gene expression databases

BgeeiENSG00000156931.
CleanExiHS_VPS8.
ExpressionAtlasiQ8N3P4. baseline and differential.
GenevisibleiQ8N3P4. HS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR011044. Quino_amine_DH_bsu.
IPR025941. Vps8_central_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF12816. Vps8. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF50969. SSF50969. 2 hits.
SSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVPS8_HUMAN
AccessioniPrimary (citable) accession number: Q8N3P4
Secondary accession number(s): A8K8Q8
, B9EIQ1, C9JB61, O94896, Q63HP2, Q9BVP9, Q9H9B0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: May 18, 2010
Last modified: September 7, 2016
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.