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Q8N3P4 (VPS8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar protein sorting-associated protein 8 homolog
Gene names
Name:VPS8
Synonyms:KIAA0804
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1428 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the VPS8 family.

Contains 1 RING-type zinc finger.

Contains 1 WD repeat.

Sequence caution

The sequence BAB14322.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence CAH56195.1 differs from that shown. Reason: Frameshift at position 325.

Isoform 2: The sequence CAH56195.1 differs from that shown. Reason: Frameshift at position 326.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainWD repeat
Zinc-finger
   LigandMetal-binding
Zinc
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionzinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8N3P4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8N3P4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     179-180: Missing.
     578-667: Missing.
Isoform 3 (identifier: Q8N3P4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     179-180: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14281428Vacuolar protein sorting-associated protein 8 homolog
PRO_0000278267

Regions

Repeat195 – 23642WD
Zinc finger1258 – 131053RING-type; atypical

Amino acid modifications

Modified residue261Phosphoserine Ref.6 Ref.7 Ref.8

Natural variations

Alternative sequence179 – 1802Missing in isoform 2 and isoform 3.
VSP_023249
Alternative sequence578 – 66790Missing in isoform 2.
VSP_023250
Natural variant831I → V. Ref.2
Corresponds to variant rs9830734 [ dbSNP | Ensembl ].
VAR_030730
Natural variant11651H → Y. Ref.2
Corresponds to variant rs11555405 [ dbSNP | Ensembl ].
VAR_030731
Natural variant13641I → T.
Corresponds to variant rs3821750 [ dbSNP | Ensembl ].
VAR_030732
Natural variant13721R → H.
Corresponds to variant rs16859527 [ dbSNP | Ensembl ].
VAR_030733

Experimental info

Sequence conflict4331E → G in CAH56195. Ref.2
Sequence conflict4711K → N in CAH56195. Ref.2
Sequence conflict7651A → S in BAB14322. Ref.1
Sequence conflict9321Missing in CAH56195. Ref.2
Sequence conflict10421T → A in BAB14322. Ref.1
Sequence conflict10431Q → L in CAH56195. Ref.2
Sequence conflict11671E → G in BAB14322. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 18, 2010. Version 3.
Checksum: 97FB5E8D453BBCB6

FASTA1,428161,754
        10         20         30         40         50         60 
MENEPDHENV EQSLCAKTSE EELNKSFNLE ASLSKFSYID MDKELEFKND LIDDKEFDIP 

        70         80         90        100        110        120 
QVDTPPTLES ILNETDDEDE SFILEDPTLL NIDTIDSHSY DTSSVASSDS GDRTNLKRKK 

       130        140        150        160        170        180 
KLPDSFSLHG SVMRHSLLKG ISAQIVSAAD KVDAGLPTAI AVSSLIAVGT SHGLALIFGK 

       190        200        210        220        230        240 
DQNQALRLCL GSTSVGGQYG AISALSINND CSRLLCGFAK GQITMWDLAS GKLLRSITDA 

       250        260        270        280        290        300 
HPPGTAILHI KFTDDPTLAI CNDSGGSVFE LTFKRVMGVR TCESRCLFSG SKGEVCCIEP 

       310        320        330        340        350        360 
LHSKPELKDH PITQFSLLAM ASLTKILVIG LKPSLKVWMT FPYGRMDPSS VPLLAWHFVA 

       370        380        390        400        410        420 
VQNYVNPMLA FCRGDVVHFL LVKRDESGAI HVTKQKHLHL YYDLINFTWI NSRTVVLLDS 

       430        440        450        460        470        480 
VEKLHVIDRQ TQEELETVEI SEVQLVYNSS HFKSLATGGN VSQALALVGE KACYQSISSY 

       490        500        510        520        530        540 
GGQIFYLGTK SVYVMMLRSW RERVDHLLKQ DCLTEALALA WSFHEGKAKA VVGLSGDASK 

       550        560        570        580        590        600 
RKAIVADRMV EILFHYADRA LKKCPDQGKI QVMEQHFQDM VPVIVDYCLL LQRKDLLFSQ 

       610        620        630        640        650        660 
MYDKLSENSV AKGVFLECLE PYILSDKLVG ITPQVMKDLI VHFQDKKLME NVEALIVHMD 

       670        680        690        700        710        720 
ITSLDIQQVV LMCWENRLYD AMIYVYNRGM NEFISPMEKL FRVIAPPLNA GKTLTDEQVV 

       730        740        750        760        770        780 
MGNKLLVYIS CCLAGRAYPL GDIPEDLVPL VKNQVFEFLI RLHSAEASPE EEIYPYIRTL 

       790        800        810        820        830        840 
LHFDTREFLN VLALTFEDFK NDKQAVEYQQ RIVDILLKVM VENSDFTPSQ VGCLFTFLAR 

       850        860        870        880        890        900 
QLAKPDNTLF VNRTLFDQVL EFLCSPDDDS RHSERQQVLL ELLQAGGIVQ FEESRLIRMA 

       910        920        930        940        950        960 
EKAEFYQICE FMYEREHQYD KIIDCYLRDP LREEEVFNYI HNILSIPGHS AEEKQSVWQK 

       970        980        990       1000       1010       1020 
AMDHIEELVS LKPCKAAELV ATHFSGHIET VIKKLQNQVL LFKFLRSLLD PREGIHVNQE 

      1030       1040       1050       1060       1070       1080 
LLQISPCITE QFIELLCQFN PTQVIETLQV LECYRLEETI QITQKYQLHE VTAYLLEKKG 

      1090       1100       1110       1120       1130       1140 
DIHGAFLIML ERLQSKLQEV THQGENTKED PSLKDVEDTM VETIALCQRN SHNLNQQQRE 

      1150       1160       1170       1180       1190       1200 
ALWFPLLEAM MAPQKLSSSA IPHLHSEALK SLTMQVLNSM AAFIALPSIL QRILQDPVYG 

      1210       1220       1230       1240       1250       1260 
KGKLGEIQGL ILGMLDTFNY EQTLLETTTS LLNQDLHWSL CNLRASVTRG LNPKQDYCSI 

      1270       1280       1290       1300       1310       1320 
CLQQYKRRQE MADEIIVFSC GHLYHSFCLQ NKECTVEFEG QTRWTCYKCS SSNKVGKLSE 

      1330       1340       1350       1360       1370       1380 
NSSEIKKGRI TPSQVKMSPS YHQSKGDPTA KKGTSEPVLD PQQIQAFDQL CRLYRGSSRL 

      1390       1400       1410       1420 
ALLTELSQNR SSESYRPFSG SQSAPAFNSI FQNENFQLQL IPPPVTED 

« Hide

Isoform 2 [UniParc].

Checksum: 67DBD2AE6D4FBEE6
Show »

FASTA1,336151,216
Isoform 3 [UniParc].

Checksum: ECF6DAC303CC7B16
Show »

FASTA1,426161,568

References

[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 608-1428 (ISOFORM 1).
Tissue: Teratocarcinoma and Testis.
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 100-1428 (ISOFORM 1), VARIANTS VAL-83 AND TYR-1165.
Tissue: Amygdala and Colon endothelium.
[3]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain and Placenta.
[5]"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 218-1428 (ISOFORM 1).
Tissue: Brain.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK022945 mRNA. Translation: BAB14322.1. Different initiation.
AK292423 mRNA. Translation: BAF85112.1.
AL833838 mRNA. Translation: CAD38698.1.
BX647915 mRNA. Translation: CAH56195.1. Frameshift.
AC025573 Genomic DNA. No translation available.
AC107294 Genomic DNA. No translation available.
AC117436 Genomic DNA. No translation available.
BC001001 mRNA. Translation: AAH01001.2.
BC140768 mRNA. Translation: AAI40769.1.
AB018347 mRNA. Translation: BAA34524.1.
CCDSCCDS46971.1. [Q8N3P4-1]
CCDS46972.1. [Q8N3P4-3]
RefSeqNP_001009921.1. NM_001009921.2. [Q8N3P4-1]
NP_056118.2. NM_015303.3. [Q8N3P4-3]
XP_005247308.1. XM_005247251.2. [Q8N3P4-1]
UniGeneHs.269263.

3D structure databases

ProteinModelPortalQ8N3P4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116937. 2 interactions.
IntActQ8N3P4. 1 interaction.
MINTMINT-1196904.
STRING9606.ENSP00000397879.

PTM databases

PhosphoSiteQ8N3P4.

Polymorphism databases

DMDM296452997.

Proteomic databases

MaxQBQ8N3P4.
PaxDbQ8N3P4.
PRIDEQ8N3P4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000436792; ENSP00000404704; ENSG00000156931. [Q8N3P4-3]
ENST00000437079; ENSP00000397879; ENSG00000156931. [Q8N3P4-1]
ENST00000446204; ENSP00000405483; ENSG00000156931. [Q8N3P4-2]
GeneID23355.
KEGGhsa:23355.
UCSCuc003fpb.1. human. [Q8N3P4-3]
uc010hyd.1. human. [Q8N3P4-2]
uc010hye.1. human. [Q8N3P4-1]

Organism-specific databases

CTD23355.
GeneCardsGC03P184529.
HGNCHGNC:29122. VPS8.
HPAHPA036871.
neXtProtNX_Q8N3P4.
PharmGKBPA142671617.
PA142671888.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG327467.
HOGENOMHOG000013171.
HOVERGENHBG071091.
InParanoidQ8N3P4.
OrthoDBEOG7KWSH0.
PhylomeDBQ8N3P4.
TreeFamTF314244.

Gene expression databases

ArrayExpressQ8N3P4.
BgeeQ8N3P4.
CleanExHS_VPS8.
GenevestigatorQ8N3P4.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProIPR011044. Quino_amine_DH_bsu.
IPR025941. Vps8_central_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF12816. Vps8. 1 hit.
[Graphical view]
SMARTSM00184. RING. 1 hit.
[Graphical view]
SUPFAMSSF50969. SSF50969. 2 hits.
SSF50978. SSF50978. 1 hit.
PROSITEPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi23355.
NextBio45370.
PROQ8N3P4.

Entry information

Entry nameVPS8_HUMAN
AccessionPrimary (citable) accession number: Q8N3P4
Secondary accession number(s): A8K8Q8 expand/collapse secondary AC list , B9EIQ1, C9JB61, O94896, Q63HP2, Q9BVP9, Q9H9B0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: May 18, 2010
Last modified: July 9, 2014
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM