Reviewed, UniProtKB/Swiss-Prot Q8N3A8 (PARP8_HUMAN)

Names and origin

Protein names

Recommended name:
    Poly [ADP-ribose] polymerase 8
      Short name=PARP-8
    EC=2.4.2.30

Gene names

Name:

PARP8

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	854 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.4
Sequence similarities	Contains 1 PARP catalytic domain.
Sequence caution	The sequence AAI05790.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	NAD
Molecular function	Glycosyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	intracellular Inferred from direct assay. Source: LIFEdb
Molecular function	NAD+ ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N3A8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N3A8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     557-598: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 854	854	Poly [ADP-ribose] polymerase 8		PRO_0000252433
Regions
	Domain	617 – 844	228	PARP catalytic
	Compositional bias	468 – 473	6	Poly-Ser
Amino acid modifications
	Modified residue	760	1	Phosphoserine Ref.4
	Modified residue	767	1	Phosphothreonine Ref.4
	Modified residue	770	1	Phosphoserine Ref.4
Natural variations
	Alternative sequence	557 – 598	42	Missing in isoform 2.		VSP_020970
	Natural variant	777	1	S → A in a colorectal cancer sample; somatic mutation. Ref.5		VAR_035853
Experimental info
	Sequence conflict	849	1	N → D in BAB15044. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: D3287F5F46BA2B63	FASTA	854	95,871
	10 20 30 40 50 60 MGMCSRQERI QKDIDVVIQK SRAEKDCLFA DFRYSDSTFT FTYVGGPRSV SYSVHVSEDY 70 80 90 100 110 120 PDNTYVSSSE NDEDVLVTTE PIPVIFHRIA TELRKTNDIN CCLSIKSKLQ KENGEESRQN 130 140 150 160 170 180 STVEEDSEGD NDSEEFYYGG QVNYDGELHK HPQLEADLSA VREIYGPHAV SLREYGAIDD 190 200 210 220 230 240 VDIDLHIDVS FLDEEIAVAW EVIRTEPIIV RLHCSLTQYL NGPVPTVDVF QISTKERFGL 250 260 270 280 290 300 GHQLKKIMQT FVTQQWKQSK EKSNCLHNKK LSEKKVKSPL HLFSTLRRSP SYPPPGCGKS 310 320 330 340 350 360 KSKLKSEQDG ISKTHKLLRR TCSSTVKTDD VCVTKSHRTF GRSLSSDPRA EQAMTAIKSH 370 380 390 400 410 420 KLLNRPCPAA VKSEECLTLK SHRLLTRSCS GDPRCEHNTN LKPHKLLSRS YSSNLRMEEL 430 440 450 460 470 480 YGLKNHKLLS KSYSSAPKSS KTELFKEPNA EGRRLSLTSG LIGILTPSSS SSSQLAPNGA 490 500 510 520 530 540 KCIPVRDRGF LVQTIEFAEQ RIPVLNEYCV VCDEPHVFQN GPMLRPTVCE RELCVFAFQT 550 560 570 580 590 600 LGVMNEAADE IATGAQVVDL LVSMCRSALE SPRKVVIFEP YPSVVDPNDP QMLAFNPRKK 610 620 630 640 650 660 NYDRVMKALD SITSIREMTQ APYLEIKKQM DKQDPLAHPL LQWVISSNRS HIVKLPVNRQ 670 680 690 700 710 720 LKFMHTPHQF LLLSSPPAKE SNFRAAKKLF GSTFAFHGSH IENWHSILRN GLVVASNTRL 730 740 750 760 770 780 QLHGAMYGSG IYLSPMSSIS FGYSGMNKKQ KVSAKDEPAS SSKSSNTSQS QKKGQQSQFL 790 800 810 820 830 840 QSRNLKCIAL CEVITSSDLH KHGEIWVVPN TDHVCTRFFF VYEDGQVGDA NINTQEGGIH 850 KEILRVIGNQ TATG
	Isoform 2. Checksum: 968394B0E0897F42	FASTA	812	91,158
	10 20 30 40 50 60 MGMCSRQERI QKDIDVVIQK SRAEKDCLFA DFRYSDSTFT FTYVGGPRSV SYSVHVSEDY 70 80 90 100 110 120 PDNTYVSSSE NDEDVLVTTE PIPVIFHRIA TELRKTNDIN CCLSIKSKLQ KENGEESRQN 130 140 150 160 170 180 STVEEDSEGD NDSEEFYYGG QVNYDGELHK HPQLEADLSA VREIYGPHAV SLREYGAIDD 190 200 210 220 230 240 VDIDLHIDVS FLDEEIAVAW EVIRTEPIIV RLHCSLTQYL NGPVPTVDVF QISTKERFGL 250 260 270 280 290 300 GHQLKKIMQT FVTQQWKQSK EKSNCLHNKK LSEKKVKSPL HLFSTLRRSP SYPPPGCGKS 310 320 330 340 350 360 KSKLKSEQDG ISKTHKLLRR TCSSTVKTDD VCVTKSHRTF GRSLSSDPRA EQAMTAIKSH 370 380 390 400 410 420 KLLNRPCPAA VKSEECLTLK SHRLLTRSCS GDPRCEHNTN LKPHKLLSRS YSSNLRMEEL 430 440 450 460 470 480 YGLKNHKLLS KSYSSAPKSS KTELFKEPNA EGRRLSLTSG LIGILTPSSS SSSQLAPNGA 490 500 510 520 530 540 KCIPVRDRGF LVQTIEFAEQ RIPVLNEYCV VCDEPHVFQN GPMLRPTVCE RELCVFAFQT 550 560 570 580 590 600 LGVMNEAADE IATGAQKKNY DRVMKALDSI TSIREMTQAP YLEIKKQMDK QDPLAHPLLQ 610 620 630 640 650 660 WVISSNRSHI VKLPVNRQLK FMHTPHQFLL LSSPPAKESN FRAAKKLFGS TFAFHGSHIE 670 680 690 700 710 720 NWHSILRNGL VVASNTRLQL HGAMYGSGIY LSPMSSISFG YSGMNKKQKV SAKDEPASSS 730 740 750 760 770 780 KSSNTSQSQK KGQQSQFLQS RNLKCIALCE VITSSDLHKH GEIWVVPNTD HVCTRFFFVY 790 800 810 EDGQVGDANI NTQEGGIHKE ILRVIGNQTA TG

References

[1]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Melanoma.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Pancreas and Skeletal muscle.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 259-854 (ISOFORM 1). Tissue: Colon.
[4]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-760; THR-767 AND SER-770, MASS SPECTROMETRY.
[5]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ALA-777.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL834477 mRNA. Translation: CAD39136.1. BC075801 mRNA. Translation: AAH75801.1. BC105789 mRNA. Translation: AAI05790.1. Sequence problems. AK024961 mRNA. Translation: BAB15044.1.
IPI	IPI00171012. IPI00795496.
RefSeq	NP_078891.2.
UniGene	Hs.369581
3D structure databases
SMR	Q8N3A8. Positions 677-745.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8N3A8.
PTM databases
PhosphoSite	Q8N3A8.
Proteomic databases
PRIDE	Q8N3A8.
Genome annotation databases
Ensembl	ENST00000281631; ENSP00000281631; ENSG00000151883; Homo sapiens. [Genome view]
GeneID	79668.
KEGG	hsa:79668.
UCSC	uc003jon.2. human. uc003jop.1. human.
Organism-specific databases
CTD	79668.
GeneCards	GC05P049998.
HGNC	HGNC:26124. PARP8.
PharmGKB	PA134903201.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG11403.
HOGENOM	HBG445795.
HOVERGEN	Q8N3A8.
InParanoid	Q8N3A8.
OMA	PGCGKSK.
OrthoDB	EOG97DD28.
PhylomeDB	Q8N3A8.
Enzyme and pathway databases
BRENDA	2.4.2.30. 247.
Gene expression databases
ArrayExpress	Q8N3A8.
Bgee	Q8N3A8.
CleanEx	HS_PARP8.
Genevestigator	Q8N3A8.
GermOnline	ENSG00000151883. Homo sapiens.
Family and domain databases
InterPro	IPR012317. Poly(ADP-ribose)pol_cat_dom. [Graphical view]
Pfam	PF00644. PARP. 1 hit. [Graphical view]
PROSITE	PS51059. PARP_CATALYTIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	68883.

Entry information

Entry name

PARP8_HUMAN

Accession

Primary (citable) accession number: Q8N3A8
Secondary accession number(s): Q3KRB7, Q6DHZ1, Q9H754

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	October 1, 2002
Last modified:	January 19, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.