Reviewed, UniProtKB/Swiss-Prot Q8N392 (RHG18_HUMAN)

Names and origin

Protein names

Recommended name:
    Rho GTPase-activating protein 18
Alternative name(s):
    Rho-type GTPase-activating protein 18
    MacGAP

Gene names

Name:

ARHGAP18

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	663 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state By similarity. UniProtKB Q9BRR9
Subunit structure	Interacts with MPHOSPH6. Ref.5
Sequence similarities	Contains 1 Rho-GAP domain.
Sequence caution	The sequence AAH39611.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence. The sequence AAH62417.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	GTPase activation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	GTPase activator activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.5 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MPHOSPH6	Q99547	1	EBI-372814,EBI-373187

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.5 Ref.3 Ref.4 (identifier: Q8N392-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.2 (identifier: Q8N392-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-45: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 663	663	Rho GTPase-activating protein 18		PRO_0000245789
Regions
	Domain	324 – 523	200	Rho-GAP
Natural variations
	Alternative sequence	1 – 45	45	Missing in isoform 2. Ref.2		VSP_052092
	Natural variant	23	1	A → T: dbSNP rs3752536.		VAR_060460
	Natural variant	91	1	N → S: dbSNP rs11544371.		VAR_060461
	Natural variant	165	1	Q → H: dbSNP rs11544372.		VAR_060462
Experimental info
	Sequence conflict	307	1	K → E in AAH17223. Ref.3
	Sequence conflict	467	1	N → K in AAH62417. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 25, 2004. Version 2. Checksum: E490D7B78E56971B	FASTA	663	74,947
	10 20 30 40 50 60 MSWLSSSQGV VLTAYHPSGK DQAVGNSHAK AGEEATSSRR YGQYTMNQES TTIKVMEKPP 70 80 90 100 110 120 FDRSISQDSL DELSMEDYWI ELENIKKSSE NSQEDQEVVV VKEPDEGELE EEWLKEAGLS 130 140 150 160 170 180 NLFGESAGDP QESIVFLSTL TRTQAAAVQK RVETVSQTLR KKNKQYQIPD VRDIFAQQRE 190 200 210 220 230 240 SKETAPGGTE SQSLRTNENK YQGRDDEASN LVGEEKLIPP EETPAPETDI NLEVSFAEQA 250 260 270 280 290 300 LNQKESSKEK IQKSKGDDAT LPSFRLPKDK TGTTRIGDLA PQDMKKVCHL ALIELTALYD 310 320 330 340 350 360 VLGIELKQQK AVKIKTKDSG LFCVPLTALL EQDQRKVPGM RIPLIFQKLI SRIEERGLET 370 380 390 400 410 420 EGLLRIPGAA IRIKNLCQEL EAKFYEGTFN WESVKQHDAA SLLKLFIREL PQPLLSVEYL 430 440 450 460 470 480 KAFQAVQNLP TKKQQLQALN LLVILLPDAN RDTLKALLEF LQRVIDNKEK NKMTVMNVAM 490 500 510 520 530 540 VMAPNLFMCH ALGLKSSEQR EFVMAAGTAN TMHLLIKYQK LLWTIPKFIV NQVRKQNTEN 550 560 570 580 590 600 HKKDKRAMKK LLKKMAYDRE KYEKQDKSTN DADVPQGVIR VQAPHLSKVS MAIQLTEELK 610 620 630 640 650 660 ASDVLARFLS QESGVAQTLK KGEVFLYEIG GNIGERCLDD DTYMKDLYQL NPNAEWVIKS KPL
	Isoform 2. Checksum: 12A738DABAFE2ABB	FASTA	618	70,164
	10 20 30 40 50 60 MNQESTTIKV MEKPPFDRSI SQDSLDELSM EDYWIELENI KKSSENSQED QEVVVVKEPD 70 80 90 100 110 120 EGELEEEWLK EAGLSNLFGE SAGDPQESIV FLSTLTRTQA AAVQKRVETV SQTLRKKNKQ 130 140 150 160 170 180 YQIPDVRDIF AQQRESKETA PGGTESQSLR TNENKYQGRD DEASNLVGEE KLIPPEETPA 190 200 210 220 230 240 PETDINLEVS FAEQALNQKE SSKEKIQKSK GDDATLPSFR LPKDKTGTTR IGDLAPQDMK 250 260 270 280 290 300 KVCHLALIEL TALYDVLGIE LKQQKAVKIK TKDSGLFCVP LTALLEQDQR KVPGMRIPLI 310 320 330 340 350 360 FQKLISRIEE RGLETEGLLR IPGAAIRIKN LCQELEAKFY EGTFNWESVK QHDAASLLKL 370 380 390 400 410 420 FIRELPQPLL SVEYLKAFQA VQNLPTKKQQ LQALNLLVIL LPDANRDTLK ALLEFLQRVI 430 440 450 460 470 480 DNKEKNKMTV MNVAMVMAPN LFMCHALGLK SSEQREFVMA AGTANTMHLL IKYQKLLWTI 490 500 510 520 530 540 PKFIVNQVRK QNTENHKKDK RAMKKLLKKM AYDREKYEKQ DKSTNDADVP QGVIRVQAPH 550 560 570 580 590 600 LSKVSMAIQL TEELKASDVL ARFLSQESGV AQTLKKGEVF LYEIGGNIGE RCLDDDTYMK 610 DLYQLNPNAE WVIKSKPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Melanoma.
[2]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 2).
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain and Uterus.
[4]	"Moleculer cloning and characterization of a novel GTPase activating Protein that regulated mast cell degranulation." Uchida T., Kuramasu A., Okumura K., Nakao A., Ogawa H., Ra C. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 39-663 (ISOFORM 1). Tissue: Mast cell.
[5]	"A protein interaction framework for human mRNA degradation." Lehner B., Sanderson C.M. Genome Res. 14:1315-1323(2004) [PubMed: 15231747] [Abstract] Cited for: INTERACTION WITH MPHOSPH6.
[6]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL834511 mRNA. Translation: CAD39167.2. AL450310 Genomic DNA. Translation: CAH72298.1. BC017223 mRNA. Translation: AAH17223.1. Sequence problems. BC039611 mRNA. Translation: AAH39611.1. Sequence problems. BC062417 mRNA. Translation: AAH62417.1. Sequence problems. BC101708 mRNA. Translation: AAI01709.1. BC107416 mRNA. Translation: AAI07417.1. BC111940 mRNA. Translation: AAI11941.1. AB053293 mRNA. Translation: BAB61887.1. Different initiation.
IPI	IPI00296353. IPI00760924.
PIR	G59432.
RefSeq	NP_277050.2.
UniGene	Hs.486458
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8N392. 1 interaction.
STRING	Q8N392.
PTM databases
PhosphoSite	Q8N392.
Proteomic databases
PRIDE	Q8N392.
Genome annotation databases
Ensembl	ENST00000275189; ENSP00000275189; ENSG00000146376; Homo sapiens. [Genome view]
GeneID	93663.
KEGG	hsa:93663.
UCSC	uc003qbr.1. human.
Organism-specific databases
CTD	93663.
GeneCards	GC06M129939.
HGNC	HGNC:21035. ARHGAP18.
PharmGKB	PA134884487.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q8N392.
HOVERGEN	Q8N392.
Enzyme and pathway databases
Reactome	REACT_11044. Signaling by Rho GTPases.
Gene expression databases
ArrayExpress	Q8N392.
Bgee	Q8N392.
CleanEx	HS_ARHGAP18.
Genevestigator	Q8N392.
GermOnline	ENSG00000146376. Homo sapiens.
Family and domain databases
InterPro	IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP. [Graphical view]
Gene3D	G3DSA:1.10.555.10. RhoGAP. 1 hit.
Pfam	PF00620. RhoGAP. 1 hit. [Graphical view]
SMART	SM00324. RhoGAP. 1 hit. [Graphical view]
PROSITE	PS50238. RHOGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	78204.

Entry information

Entry name

RHG18_HUMAN

Accession

Primary (citable) accession number: Q8N392
Secondary accession number(s): Q58EZ3 , Q6P679, Q6PJD7, Q96S64

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	October 25, 2004
Last modified:	November 24, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.