Q8N357 (S35F6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Solute carrier family 35 member F6

Alternative name(s):
ANT2-binding protein
Short name=ANT2BP
Transport and Golgi organization 9 homolog

Gene names

Name:	SLC35F6
Synonyms:	C2orf18
ORF Names:	UNQ3047/PRO9863

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the maintenance of mitochondrial membrane potential in pancreatic ductal adenocarcinoma (PDAC) cells. Promotes pancreatic ductal adenocarcinoma (PDAC) cell growth. May play a role as a nucleotide-sugar transporter. Ref.11
Subunit structure	Interacts with SLC25A5.
Subcellular location	Mitochondrion. Lysosome membrane; Multi-pass membrane protein Ref.11 Ref.12.
Tissue specificity	Expressed in pancreatic ductal adenocarcinoma (PDAC) (at protein level). Strongly expressed in prostate and thyroid. Weakly expressed in lung, heart, liver and kidney. Ref.11
Sequence similarities	Belongs to the SLC35F solute transporter family. Contains 1 EamA domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Lysosome Membrane Mitochondrion
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of mitochondrial outer membrane permeabilization Inferred from mutant phenotype Ref.11. Source: UniProtKB positive regulation of cell proliferation Inferred from mutant phenotype Ref.11. Source: UniProtKB transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from direct assay. Source: HPA integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lysosomal membrane Inferred from direct assay Ref.12. Source: UniProtKB mitochondrion Inferred from direct assay Ref.11. Source: UniProtKB nucleus Inferred from direct assay. Source: HPA
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SLC25A5	P05141	2	EBI-713484,EBI-355133

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 371	353	Solute carrier family 35 member F6		PRO_0000232514
Regions
	Transmembrane	48 – 68	21	Helical; Potential
	Transmembrane	89 – 109	21	Helical; Potential
	Transmembrane	117 – 137	21	Helical; Potential
	Transmembrane	140 – 160	21	Helical; Potential
	Transmembrane	176 – 196	21	Helical; Potential
	Transmembrane	216 – 236	21	Helical; Potential
	Transmembrane	261 – 281	21	Helical; Potential
	Transmembrane	295 – 312	18	Helical; Potential
	Transmembrane	317 – 336	20	Helical; Potential
	Domain	104 – 160	57	EamA
Amino acid modifications
	Modified residue	365	1	Phosphothreonine Ref.10
	Glycosylation	110	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	107	1	V → A in BAD96649. Ref.5
	Sequence conflict	163	1	L → P in BAD96649. Ref.5
	Sequence conflict	215	1	E → K in BAA91255. Ref.2
	Sequence conflict	274	1	A → V in BAD96649. Ref.5
	Sequence conflict	295	1	L → S in BAC11445. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8N357 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 225AFE41FA555B12	FASTA	371	40,214
	10 20 30 40 50 60 MAWTKYQLFL AGLMLVTGSI NTLSAKWADN FMAEGCGGSK EHSFQHPFLQ AVGMFLGEFS 70 80 90 100 110 120 CLAAFYLLRC RAAGQSDSSV DPQQPFNPLL FLPPALCDMT GTSLMYVALN MTSASSFQML 130 140 150 160 170 180 RGAVIIFTGL FSVAFLGRRL VLSQWLGILA TIAGLVVVGL ADLLSKHDSQ HKLSEVITGD 190 200 210 220 230 240 LLIIMAQIIV AIQMVLEEKF VYKHNVHPLR AVGTEGLFGF VILSLLLVPM YYIPAGSFSG 250 260 270 280 290 300 NPRGTLEDAL DAFCQVGQQP LIAVALLGNI SSIAFFNFAG ISVTKELSAT TRMVLDSLRT 310 320 330 340 350 360 VVIWALSLAL GWEAFHALQI LGFLILLIGT ALYNGLHRPL LGRLSRGRPL AEESEQERLL 370 GGTRTPINDA S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[6]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood.
[9]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-365, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Identification of C2orf18, termed ANT2BP (ANT2-binding protein), as one of the key molecules involved in pancreatic carcinogenesis." Kashiwaya K., Hosokawa M., Eguchi H., Ohigashi H., Ishikawa O., Shinomura Y., Nakamura Y., Nakagawa H. Cancer Sci. 100:457-464(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTS WITH SLC25A5, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[12]	"The proteome of lysosomes." Schroeder B.A., Wrocklage C., Hasilik A., Saftig P. Proteomics 10:4053-4076(2010) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW, SUBCELLULAR LOCATION.
[13]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY358820 mRNA. Translation: AAQ89179.1. AK000562 mRNA. Translation: BAA91255.1. AK075164 mRNA. Translation: BAC11445.1. CR457243 mRNA. Translation: CAG33524.1. AK222929 mRNA. Translation: BAD96649.1. AC011740 Genomic DNA. Translation: AAX93265.1. CH471053 Genomic DNA. Translation: EAX00673.1. CH471053 Genomic DNA. Translation: EAX00676.1. BC028081 mRNA. Translation: AAH28081.1.
IPI	IPI00550440.
RefSeq	NP_060347.2. NM_017877.3.
UniGene	Hs.1594. Hs.516034.
3D structure databases
ProteinModelPortal	Q8N357.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8N357. 16 interactions.
MINT	MINT-1383766.
STRING	9606.ENSP00000345528.
Protein family/group databases
TCDB	2.A.7.12.13. drug/metabolite transporter (DMT) superfamily.
PTM databases
PhosphoSite	Q8N357.
Polymorphism databases
DMDM	74728772.
Proteomic databases
PaxDb	Q8N357.
PRIDE	Q8N357.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000344420; ENSP00000345528; ENSG00000213699.
GeneID	54978.
KEGG	hsa:54978.
UCSC	uc002rhp.1. human.
Organism-specific databases
CTD	54978.
GeneCards	GC02P026987.
HGNC	HGNC:26055. SLC35F6.
HPA	HPA034655.
neXtProt	NX_Q8N357.
PharmGKB	PA134913368.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0697.
HOGENOM	HOG000210249.
HOVERGEN	HBG057690.
InParanoid	Q8N357.
OMA	WEAFHPL.
PhylomeDB	Q8N357.
Gene expression databases
ArrayExpress	Q8N357.
Bgee	Q8N357.
CleanEx	HS_C2orf18.
Genevestigator	Q8N357.
GermOnline	ENSG00000115163. Homo sapiens.
Family and domain databases
InterPro	IPR009262. DUF914_euk. IPR012404. UCP036436. [Graphical view]
Pfam	PF06027. DUF914. 1 hit. [Graphical view]
PIRSF	PIRSF036436. UCP036436. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	54978.
NextBio	58238.

Entry information

Entry name

S35F6_HUMAN

Accession

Primary (citable) accession number: Q8N357
Secondary accession number(s): D6W543 , Q53GK2, Q8NBX6, Q9NWX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.