ID   Q8N355_HUMAN            Unreviewed;       234 AA.
AC   Q8N355;
DT   01-OCT-2002, integrated into UniProtKB/TrEMBL.
DT   01-OCT-2002, sequence version 1.
DT   27-MAR-2024, entry version 150.
DE   SubName: Full=IGL@ protein {ECO:0000313|EMBL:AAH28090.1};
GN   Name=IGL@ {ECO:0000313|EMBL:AAH28090.1};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606 {ECO:0000313|EMBL:AAH28090.1};
RN   [1] {ECO:0000313|EMBL:AAH28090.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain {ECO:0000313|EMBL:AAH28090.1};
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RA   Gerhard D.S., Wagner L., Feingold E.A., Shenmen C.M., Grouse L.H.,
RA   Schuler G., Klein S.L., Old S., Rasooly R., Good P., Guyer M., Peck A.M.,
RA   Derge J.G., Lipman D., Collins F.S., Jang W., Sherry S., Feolo M.,
RA   Misquitta L., Lee E., Rotmistrovsky K., Greenhut S.F., Schaefer C.F.,
RA   Buetow K., Bonner T.I., Haussler D., Kent J., Kiekhaus M., Furey T.,
RA   Brent M., Prange C., Schreiber K., Shapiro N., Bhat N.K., Hopkins R.F.,
RA   Hsie F., Driscoll T., Soares M.B., Casavant T.L., Scheetz T.E.,
RA   Brown-stein M.J., Usdin T.B., Toshiyuki S., Carninci P., Piao Y.,
RA   Dudekula D.B., Ko M.S., Kawakami K., Suzuki Y., Sugano S., Gruber C.E.,
RA   Smith M.R., Simmons B., Moore T., Waterman R., Johnson S.L., Ruan Y.,
RA   Wei C.L., Mathavan S., Gunaratne P.H., Wu J., Garcia A.M., Hulyk S.W.,
RA   Fuh E., Yuan Y., Sneed A., Kowis C., Hodgson A., Muzny D.M., McPherson J.,
RA   Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Madari A., Young A.C., Wetherby K.D., Granite S.J.,
RA   Kwong P.N., Brinkley C.P., Pearson R.L., Bouffard G.G., Blakesly R.W.,
RA   Green E.D., Dickson M.C., Rodriguez A.C., Grimwood J., Schmutz J.,
RA   Myers R.M., Butterfield Y.S., Griffith M., Griffith O.L., Krzywinski M.I.,
RA   Liao N., Morin R., Morrin R., Palmquist D., Petrescu A.S., Skalska U.,
RA   Smailus D.E., Stott J.M., Schnerch A., Schein J.E., Jones S.J., Holt R.A.,
RA   Baross A., Marra M.A., Clifton S., Makowski K.A., Bosak S., Malek J.;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [2] {ECO:0007829|PDB:2DD8}
RP   X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 20-234, AND DISULFIDE BONDS.
RX   PubMed=16597622; DOI=10.1074/jbc.m600697200;
RA   Prabakaran P., Gan J., Feng Y., Zhu Z., Choudhry V., Xiao X., Ji X.,
RA   Dimitrov D.S.;
RT   "Structure of severe acute respiratory syndrome coronavirus receptor-
RT   binding domain complexed with neutralizing antibody.";
RL   J. Biol. Chem. 281:15829-15836(2006).
RN   [3] {ECO:0007829|PDB:5UGY}
RP   X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) OF 22-231, AND DISULFIDE BONDS.
RX   PubMed=21825125; DOI=10.1073/pnas.1111497108;
RA   Whittle J.R., Zhang R., Khurana S., King L.R., Manischewitz J., Golding H.,
RA   Dormitzer P.R., Haynes B.F., Walter E.B., Moody M.A., Kepler T.B.,
RA   Liao H.X., Harrison S.C.;
RT   "Broadly neutralizing human antibody that recognizes the receptor-binding
RT   pocket of influenza virus hemagglutinin.";
RL   Proc. Natl. Acad. Sci. U.S.A. 108:14216-14221(2011).
RN   [4] {ECO:0007829|PDB:4FQQ}
RP   X-RAY CRYSTALLOGRAPHY (2.42 ANGSTROMS) OF 20-234, AND DISULFIDE BONDS.
RX   PubMed=23115339; DOI=10.1073/pnas.1217207109;
RA   Mouquet H., Scharf L., Euler Z., Liu Y., Eden C., Scheid J.F.,
RA   Halper-Stromberg A., Gnanapragasam P.N., Spencer D.I., Seaman M.S.,
RA   Schuitemaker H., Feizi T., Nussenzweig M.C., Bjorkman P.J.;
RT   "Complex-type N-glycan recognition by potent broadly neutralizing HIV
RT   antibodies.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:E3268-E3277(2012).
RN   [5] {ECO:0007829|PDB:4HK0, ECO:0007829|PDB:4HK3}
RP   X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 22-234, AND DISULFIDE BONDS.
RX   PubMed=23175789; DOI=10.1073/pnas.1218256109;
RA   Schmidt A.G., Xu H., Khan A.R., O'Donnell T., Khurana S., King L.R.,
RA   Manischewitz J., Golding H., Suphaphiphat P., Carfi A., Settembre E.C.,
RA   Dormitzer P.R., Kepler T.B., Zhang R., Moody M.A., Haynes B.F., Liao H.X.,
RA   Shaw D.E., Harrison S.C.;
RT   "Preconfiguration of the antigen-binding site during affinity maturation of
RT   a broadly neutralizing influenza virus antibody.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:264-269(2013).
RN   [6] {ECO:0007829|PDB:6DC3}
RP   X-RAY CRYSTALLOGRAPHY (3.50 ANGSTROMS) OF 22-234, AND DISULFIDE BONDS.
RX   PubMed=31306469; DOI=10.1371/journal.ppat.1007944;
RA   Jones H.G., Battles M.B., Lin C.C., Bianchi S., Corti D., McLellan J.S.;
RT   "Alternative conformations of a major antigenic site on RSV F.";
RL   PLoS Pathog. 15:e1007944-e1007944(2019).
RN   [7] {ECO:0007829|PDB:6UDJ, ECO:0007829|PDB:6UDK}
RP   STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF 20-234, AND DISULFIDE
RP   BONDS.
RX   PubMed=32004464; DOI=10.1016/j.cell.2020.01.010;
RA   Schommers P., Gruell H., Abernathy M.E., Tran M.K., Dingens A.S.,
RA   Gristick H.B., Barnes C.O., Schoofs T., Schlotz M., Vanshylla K., Kreer C.,
RA   Weiland D., Holtick U., Scheid C., Valter M.M., van Gils M.J.,
RA   Sanders R.W., Vehreschild J.J., Cornely O.A., Lehmann C., Fatkenheuer G.,
RA   Seaman M.S., Bloom J.D., Bjorkman P.J., Klein F.;
RT   "Restriction of HIV-1 Escape by a Highly Broad and Potent Neutralizing
RT   Antibody.";
RL   Cell 180:471-489.e22(2020).
RN   [8] {ECO:0007829|PDB:6VPY}
RP   X-RAY CRYSTALLOGRAPHY (2.36 ANGSTROMS) OF 20-234, AND DISULFIDE BONDS.
RX   PubMed=32765530; DOI=10.3389/fimmu.2020.01529;
RA   Zhou J.O., Zaidi H.A., Ton T., Fera D.;
RT   "The Effects of Framework Mutations at the Variable Domain Interface on
RT   Antibody Affinity Maturation in an HIV-1 Broadly Neutralizing Antibody
RT   Lineage.";
RL   Front. Immunol. 11:1529-1529(2020).
CC   -!- INTERACTION:
CC       Q8N355; O43765: SGTA; NbExp=4; IntAct=EBI-748681, EBI-347996;
CC       Q8N355; Q9UMX0: UBQLN1; NbExp=4; IntAct=EBI-748681, EBI-741480;
CC       Q8N355; Q9UMX0-2: UBQLN1; NbExp=3; IntAct=EBI-748681, EBI-10173939;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC028090; AAH28090.1; -; mRNA.
DR   PIR; S09713; S09713.
DR   PIR; S12441; S12441.
DR   PIR; S30527; S30527.
DR   PDB; 2DD8; X-ray; 2.30 A; L=20-234.
DR   PDB; 4FQQ; X-ray; 2.42 A; A/C/E/L=20-234.
DR   PDB; 4HK0; X-ray; 2.50 A; B/D=22-234.
DR   PDB; 4HK3; X-ray; 3.00 A; N=22-234.
DR   PDB; 5UGY; X-ray; 2.80 A; L/M/N=22-231.
DR   PDB; 6DC3; X-ray; 3.50 A; L=22-234.
DR   PDB; 6UDJ; EM; 2.50 A; B/L/R=20-234.
DR   PDB; 6UDK; EM; 3.90 A; B/I/P=20-234.
DR   PDB; 6VPY; X-ray; 2.36 A; B/L=20-234.
DR   PDBsum; 4FQQ; -.
DR   PDBsum; 4HK0; -.
DR   PDBsum; 4HK3; -.
DR   PDBsum; 6UDJ; -.
DR   PDBsum; 6UDK; -.
DR   AlphaFoldDB; Q8N355; -.
DR   SMR; Q8N355; -.
DR   IntAct; Q8N355; 5.
DR   MaxQB; Q8N355; -.
DR   ChiTaRS; IGL; human.
DR   CDD; cd07699; IgC1_L; 1.
DR   CDD; cd04984; IgV_L_lambda; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR   InterPro; IPR007110; Ig-like_dom.
DR   InterPro; IPR036179; Ig-like_dom_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR003006; Ig/MHC_CS.
DR   InterPro; IPR003597; Ig_C1-set.
DR   InterPro; IPR003599; Ig_sub.
DR   InterPro; IPR013106; Ig_V-set.
DR   PANTHER; PTHR19944:SF98; IG-LIKE DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR19944; MHC CLASS II-RELATED; 1.
DR   Pfam; PF07654; C1-set; 1.
DR   Pfam; PF07686; V-set; 1.
DR   SMART; SM00409; IG; 2.
DR   SMART; SM00407; IGc1; 1.
DR   SMART; SM00406; IGv; 1.
DR   SUPFAM; SSF48726; Immunoglobulin; 2.
DR   PROSITE; PS50835; IG_LIKE; 2.
DR   PROSITE; PS00290; IG_MHC; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:2DD8, ECO:0007829|PDB:4FQQ};
KW   Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           17..234
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5004314219"
FT   DOMAIN          20..108
FT                   /note="Ig-like"
FT                   /evidence="ECO:0000259|PROSITE:PS50835"
FT   DOMAIN          135..229
FT                   /note="Ig-like"
FT                   /evidence="ECO:0000259|PROSITE:PS50835"
FT   DISULFID        41..106
FT                   /evidence="ECO:0007829|PDB:2DD8, ECO:0007829|PDB:4FQQ"
FT   DISULFID        156..215
FT                   /evidence="ECO:0007829|PDB:2DD8, ECO:0007829|PDB:4FQQ"
SQ   SEQUENCE   234 AA;  24793 MW;  CC848CAEBA4A9D63 CRC64;
     MAWTVLLLGL LSHCTGSVTS YVLTQPPSVS VAPGQTARIT CGGNNIGSKS VHWYQQKPGQ
     APVLVVYDDS DRPSGIPERF SGSNSGNTAT LTISRVDAGD EADYYCQLWD SSSDHPVVFG
     GGTKLTVLGQ PKAAPSVTLF PPSSEELQAN KATLVCLISD FYPGAVTVAW KADSSPVKAG
     VETTTPSKQS NNKYAASSYL SLTPEQWKSH RSYSCQVTHE GSTVEKTVAP TECS
//