Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Centromere protein S

Gene

APITD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding component of the FA core complex involved in DNA damage repair and genome maintenance. Required for optimal chromatin association of the FA core complex. Required for efficient damage-induced monoubiquitination and focus formation of FANCD2. Stabilizes FAAD24, FANCM and STRA13/CENPX in the FA core complex. Plays a role in DNA interstrand cross-linking (ICL) repair and in recovery of replication forks stalled by topoisomerase I-DNA cleavage intermediates induced by camptothecin. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. Component of the APITD1/CENPS complex that is essential for the stable assembly of the outer kinetochore. Plays an important role in mitotic progression and chromosome segregation. Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation.3 Publications

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • DNA binding Source: UniProtKB

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • cellular response to DNA damage stimulus Source: UniProtKB
  • CENP-A containing nucleosome assembly Source: Reactome
  • DNA repair Source: UniProtKB
  • interstrand cross-link repair Source: GO_Central
  • mitotic nuclear division Source: UniProtKB-KW
  • mitotic recombination Source: GO_Central
  • positive regulation of protein ubiquitination Source: UniProtKB
  • replication fork processing Source: UniProtKB
  • resolution of meiotic recombination intermediates Source: UniProtKB
  • sister chromatid cohesion Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA repair, Mitosis

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-606279. Deposition of new CENPA-containing nucleosomes at the centromere.
R-HSA-6783310. Fanconi Anemia Pathway.
R-HSA-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein S
Short name:
CENP-S
Alternative name(s):
Apoptosis-inducing TAF9-like domain-containing protein 1
FANCM-interacting histone fold protein 1
Fanconi anemia-associated polypeptide of 16 kDa
Gene namesi
Name:APITD1
Synonyms:CENPS, FAAP16, MHF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:23163. APITD1.

Subcellular locationi

GO - Cellular componenti

  • condensed chromosome kinetochore Source: UniProtKB-SubCell
  • cytosol Source: Reactome
  • FANCM-MHF complex Source: UniProtKB
  • Fanconi anaemia nuclear complex Source: UniProtKB
  • nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Fanconi anemia

Organism-specific databases

PharmGKBiPA134861614.

Polymorphism and mutation databases

BioMutaiAPITD1.
DMDMi74759833.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 138138Centromere protein SPRO_0000249477Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8N2Z9.
MaxQBiQ8N2Z9.
PaxDbiQ8N2Z9.
PeptideAtlasiQ8N2Z9.
PRIDEiQ8N2Z9.

PTM databases

iPTMnetiQ8N2Z9.
PhosphoSiteiQ8N2Z9.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000175279.
ExpressionAtlasiQ8N2Z9. baseline and differential.
GenevisibleiQ8N2Z9. HS.

Interactioni

Subunit structurei

Component of a discrete APITD1/CENPS complex composed of at least APITD1/CENPS and STRA13/CENPX. Belongs to the multisubunit FA complex composed of APITD1/CENPS, FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9, FANCM, FAAP24 and STRA13/CENPX. Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and APITD1/CENPS. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. Interacts with APITD1/CENPS, FANCM and FAAP24. Binds DNA. Component of a heterotetrameric CENP-T-W-S-X complex composed of APITD1/CENPS, STRA13/CENPX, CENPT and CENPW.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HOMEZQ8IX15-33EBI-5529649,EBI-10172004
STRA13A8MT696EBI-5529649,EBI-5529694

Protein-protein interaction databases

BioGridi132044. 21 interactions.
1529300. 1 interaction.
IntActiQ8N2Z9. 6 interactions.
STRINGi9606.ENSP00000308583.

Structurei

Secondary structure

1
138
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 125Combined sources
Helixi15 – 3824Combined sources
Helixi44 – 7128Combined sources
Beta strandi75 – 773Combined sources
Helixi79 – 857Combined sources
Turni86 – 883Combined sources
Helixi90 – 10314Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DRAX-ray2.41A/B/C/D1-107[»]
4DRBX-ray2.63A/B/D/E/G/H1-114[»]
4E44X-ray2.10A/C1-110[»]
4E45X-ray2.00A/C/F/H/K/M1-110[»]
4NDYX-ray7.00A/C/G/I/J/K/Q/R/S/T14-105[»]
4NE1X-ray6.50A/C/G/I/J/K/Q/R/S/T/Y/a/c/e/f/g/k/l/m/n14-118[»]
4NE3X-ray1.80A14-105[»]
4NE5X-ray2.50A/C/E/G14-105[»]
4NE6X-ray2.10A/C14-105[»]
ProteinModelPortaliQ8N2Z9.
SMRiQ8N2Z9. Positions 4-105.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TAF9 family. CENPS subfamily.Curated

Phylogenomic databases

eggNOGiENOG410J11U. Eukaryota.
ENOG410XX38. LUCA.
GeneTreeiENSGT00510000048007.
HOGENOMiHOG000216554.
HOVERGENiHBG080286.
InParanoidiQ8N2Z9.
KOiK11511.
OMAiEITFRQC.
OrthoDBiEOG091G19D0.
PhylomeDBiQ8N2Z9.
TreeFamiTF300253.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR029003. CENP-S/Mhf1.
IPR033554. CENPS.
IPR009072. Histone-fold.
[Graphical view]
PANTHERiPTHR22980:SF4. PTHR22980:SF4. 1 hit.
PfamiPF15630. CENP-S. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N2Z9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEEAETEEQ QRFSYQQRLK AAVHYTVGCL CEEVALDKEM QFSKQTIAAI
60 70 80 90 100
SELTFRQCEN FAKDLEMFAR HAKRTTINTE DVKLLARRSN SLLKYITDKS
110 120 130
EEIAQINLER KAQKKKKSED GSKNSRQPAE AGVVESEN
Length:138
Mass (Da):15,893
Last modified:October 1, 2002 - v1
Checksum:iCBCD22CDDE213D87
GO
Isoform 2 (identifier: Q8N2Z9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     91-138: SLLKYITDKS...AEAGVVESEN → SLHMQEAAGI...FFWKTFSSCK

Note: No experimental confirmation available.
Show »
Length:164
Mass (Da):19,048
Checksum:i389682C41252426A
GO
Isoform 3 (identifier: Q8N2Z9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     70-77: RHAKRTTI → SICRKRQE
     78-138: Missing.

Note: No experimental confirmation available.
Show »
Length:77
Mass (Da):9,074
Checksum:i447AD9A2366361C1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei70 – 778RHAKRTTI → SICRKRQE in isoform 3. 1 PublicationVSP_020432
Alternative sequencei78 – 13861Missing in isoform 3. 1 PublicationVSP_020433Add
BLAST
Alternative sequencei91 – 13848SLLKY…VESEN → SLHMQEAAGIRKSSLLTFLA WWFEWTSQASAGPLIGEEAR EVARRQEGAPPQQSARRDRM PCRNFFWKTFSSCK in isoform 2. 1 PublicationVSP_020434Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF516753 mRNA. Translation: AAM61954.1.
AF521016 mRNA. Translation: AAM70482.1.
AL139424, AL354956 Genomic DNA. Translation: CAI95756.1.
AL354956, AL139424 Genomic DNA. Translation: CAI19200.1.
AL139424, AL354956 Genomic DNA. Translation: CAI95757.1.
AL354956, AL139424 Genomic DNA. Translation: CAI19201.1.
AL139424, AL354956 Genomic DNA. Translation: CAI95755.1.
AL354956, AL139424 Genomic DNA. Translation: CAI19199.1.
BC029430 mRNA. Translation: AAH29430.1.
CCDSiCCDS115.1. [Q8N2Z9-1]
RefSeqiNP_001257446.1. NM_001270517.1. [Q8N2Z9-1]
NP_940946.1. NM_198544.3. [Q8N2Z9-2]
NP_950171.2. NM_199006.2. [Q8N2Z9-3]
NP_954988.1. NM_199294.2. [Q8N2Z9-1]
UniGeneiHs.412311.

Genome annotation databases

EnsembliENST00000309048; ENSP00000308583; ENSG00000175279. [Q8N2Z9-1]
GeneIDi100526739.
378708.
KEGGihsa:100526739.
hsa:378708.
UCSCiuc001are.4. human. [Q8N2Z9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF516753 mRNA. Translation: AAM61954.1.
AF521016 mRNA. Translation: AAM70482.1.
AL139424, AL354956 Genomic DNA. Translation: CAI95756.1.
AL354956, AL139424 Genomic DNA. Translation: CAI19200.1.
AL139424, AL354956 Genomic DNA. Translation: CAI95757.1.
AL354956, AL139424 Genomic DNA. Translation: CAI19201.1.
AL139424, AL354956 Genomic DNA. Translation: CAI95755.1.
AL354956, AL139424 Genomic DNA. Translation: CAI19199.1.
BC029430 mRNA. Translation: AAH29430.1.
CCDSiCCDS115.1. [Q8N2Z9-1]
RefSeqiNP_001257446.1. NM_001270517.1. [Q8N2Z9-1]
NP_940946.1. NM_198544.3. [Q8N2Z9-2]
NP_950171.2. NM_199006.2. [Q8N2Z9-3]
NP_954988.1. NM_199294.2. [Q8N2Z9-1]
UniGeneiHs.412311.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DRAX-ray2.41A/B/C/D1-107[»]
4DRBX-ray2.63A/B/D/E/G/H1-114[»]
4E44X-ray2.10A/C1-110[»]
4E45X-ray2.00A/C/F/H/K/M1-110[»]
4NDYX-ray7.00A/C/G/I/J/K/Q/R/S/T14-105[»]
4NE1X-ray6.50A/C/G/I/J/K/Q/R/S/T/Y/a/c/e/f/g/k/l/m/n14-118[»]
4NE3X-ray1.80A14-105[»]
4NE5X-ray2.50A/C/E/G14-105[»]
4NE6X-ray2.10A/C14-105[»]
ProteinModelPortaliQ8N2Z9.
SMRiQ8N2Z9. Positions 4-105.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi132044. 21 interactions.
1529300. 1 interaction.
IntActiQ8N2Z9. 6 interactions.
STRINGi9606.ENSP00000308583.

PTM databases

iPTMnetiQ8N2Z9.
PhosphoSiteiQ8N2Z9.

Polymorphism and mutation databases

BioMutaiAPITD1.
DMDMi74759833.

Proteomic databases

EPDiQ8N2Z9.
MaxQBiQ8N2Z9.
PaxDbiQ8N2Z9.
PeptideAtlasiQ8N2Z9.
PRIDEiQ8N2Z9.

Protocols and materials databases

DNASUi378708.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000309048; ENSP00000308583; ENSG00000175279. [Q8N2Z9-1]
GeneIDi100526739.
378708.
KEGGihsa:100526739.
hsa:378708.
UCSCiuc001are.4. human. [Q8N2Z9-1]

Organism-specific databases

CTDi100526739.
378708.
GeneCardsiAPITD1.
HGNCiHGNC:23163. APITD1.
MIMi609130. gene.
neXtProtiNX_Q8N2Z9.
PharmGKBiPA134861614.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J11U. Eukaryota.
ENOG410XX38. LUCA.
GeneTreeiENSGT00510000048007.
HOGENOMiHOG000216554.
HOVERGENiHBG080286.
InParanoidiQ8N2Z9.
KOiK11511.
OMAiEITFRQC.
OrthoDBiEOG091G19D0.
PhylomeDBiQ8N2Z9.
TreeFamiTF300253.

Enzyme and pathway databases

ReactomeiR-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-606279. Deposition of new CENPA-containing nucleosomes at the centromere.
R-HSA-6783310. Fanconi Anemia Pathway.
R-HSA-68877. Mitotic Prometaphase.

Miscellaneous databases

PROiQ8N2Z9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000175279.
ExpressionAtlasiQ8N2Z9. baseline and differential.
GenevisibleiQ8N2Z9. HS.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR029003. CENP-S/Mhf1.
IPR033554. CENPS.
IPR009072. Histone-fold.
[Graphical view]
PANTHERiPTHR22980:SF4. PTHR22980:SF4. 1 hit.
PfamiPF15630. CENP-S. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCENPS_HUMAN
AccessioniPrimary (citable) accession number: Q8N2Z9
Secondary accession number(s): Q8NFE5, Q8NFG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: October 1, 2002
Last modified: September 7, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.