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Protein

Neuroligin-1

Gene

NLGN1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Required to maintain wakefulness quality and normal synchrony of cerebral cortex activity during wakefulness and sleep.By similarity

GO - Molecular functioni

  • amyloid-beta binding Source: ARUK-UCL
  • carboxylic ester hydrolase activity Source: GO_Central
  • cell adhesion molecule binding Source: BHF-UCL
  • neurexin family protein binding Source: UniProtKB
  • PDZ domain binding Source: UniProtKB
  • protein dimerization activity Source: BHF-UCL
  • receptor activity Source: BHF-UCL
  • scaffold protein binding Source: BHF-UCL

GO - Biological processi

Keywordsi

Biological processBiological rhythms, Cell adhesion

Enzyme and pathway databases

ReactomeiR-HSA-6794361. Neurexins and neuroligins.

Protein family/group databases

ESTHERihuman-NLGN1. Neuroligin.
MEROPSiS09.994.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroligin-1
Gene namesi
Name:NLGN1
Synonyms:KIAA1070
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000169760.17.
HGNCiHGNC:14291. NLGN1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini46 – 694ExtracellularSequence analysisAdd BLAST649
Transmembranei695 – 715HelicalSequence analysisAdd BLAST21
Topological domaini716 – 840CytoplasmicSequence analysisAdd BLAST125

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Organism-specific databases

DisGeNETi22871.
OpenTargetsiENSG00000169760.
PharmGKBiPA31647.

Polymorphism and mutation databases

DMDMi31076822.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 45Sequence analysisAdd BLAST45
ChainiPRO_000000864046 – 840Neuroligin-1Add BLAST795

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi109N-linked (GlcNAc...) (complex) asparagineBy similarity1
Disulfide bondi117 ↔ 153By similarity
Glycosylationi300N-linked (GlcNAc...) (complex) asparagineBy similarity1
Disulfide bondi339 ↔ 350By similarity
Glycosylationi340N-linked (GlcNAc...) (complex) asparagineBy similarity1
Disulfide bondi509 ↔ 543By similarity
Glycosylationi544N-linked (GlcNAc...) asparagineBy similarity1
Glycosylationi680O-linked (GalNAc...) serineBy similarity1
Glycosylationi683O-linked (GalNAc...) serineBy similarity1
Modified residuei730PhosphoserineBy similarity1
Modified residuei779PhosphotyrosineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8N2Q7.
PeptideAtlasiQ8N2Q7.
PRIDEiQ8N2Q7.

PTM databases

iPTMnetiQ8N2Q7.
PhosphoSitePlusiQ8N2Q7.

Expressioni

Tissue specificityi

Expressed in the blood vessel walls (at protein level). Detected in brain, and at lower levels in pancreas islet beta cells.2 Publications

Gene expression databases

BgeeiENSG00000169760.
CleanExiHS_NLGN1.
ExpressionAtlasiQ8N2Q7. baseline and differential.
GenevisibleiQ8N2Q7. HS.

Organism-specific databases

HPAiHPA006572.
HPA006680.

Interactioni

Subunit structurei

Interacts with NRXN1, NRXN2 and NRXN3. Interacts with NLGN3. Interacts with AIP1 and PDZRN3 (By similarity). Interacts (via its C-terminus) with DLG4/PSD-95 (via PDZ domain 3). Interacts with GOPC.By similarity2 Publications

GO - Molecular functioni

  • cell adhesion molecule binding Source: BHF-UCL
  • neurexin family protein binding Source: UniProtKB
  • PDZ domain binding Source: UniProtKB
  • protein dimerization activity Source: BHF-UCL
  • scaffold protein binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi116538. 12 interactors.
IntActiQ8N2Q7. 5 interactors.
MINTiMINT-1901478.
STRINGi9606.ENSP00000354541.

Structurei

Secondary structure

1840
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi54 – 57Combined sources4
Beta strandi60 – 63Combined sources4
Beta strandi65 – 67Combined sources3
Beta strandi72 – 74Combined sources3
Beta strandi77 – 84Combined sources8
Helixi91 – 93Combined sources3
Beta strandi105 – 109Combined sources5
Beta strandi122 – 125Combined sources4
Turni128 – 130Combined sources3
Helixi133 – 137Combined sources5
Helixi139 – 144Combined sources6
Beta strandi147 – 149Combined sources3
Beta strandi155 – 160Combined sources6
Beta strandi190 – 195Combined sources6
Beta strandi199 – 203Combined sources5
Helixi206 – 208Combined sources3
Helixi212 – 218Combined sources7
Beta strandi220 – 225Combined sources6
Helixi230 – 234Combined sources5
Beta strandi238 – 241Combined sources4
Helixi246 – 261Combined sources16
Helixi262 – 265Combined sources4
Beta strandi267 – 277Combined sources11
Helixi279 – 288Combined sources10
Helixi291 – 293Combined sources3
Beta strandi304 – 313Combined sources10
Beta strandi316 – 318Combined sources3
Helixi326 – 336Combined sources11
Helixi344 – 351Combined sources8
Helixi356 – 360Combined sources5
Beta strandi372 – 374Combined sources3
Beta strandi379 – 382Combined sources4
Helixi386 – 391Combined sources6
Beta strandi399 – 405Combined sources7
Turni406 – 409Combined sources4
Helixi410 – 413Combined sources4
Turni414 – 416Combined sources3
Beta strandi419 – 421Combined sources3
Helixi425 – 439Combined sources15
Beta strandi443 – 445Combined sources3
Helixi446 – 456Combined sources11
Helixi460 – 462Combined sources3
Helixi466 – 481Combined sources16
Helixi483 – 495Combined sources13
Beta strandi500 – 505Combined sources6
Turni522 – 525Combined sources4
Helixi526 – 530Combined sources5
Helixi532 – 535Combined sources4
Beta strandi539 – 541Combined sources3
Helixi547 – 566Combined sources20
Beta strandi571 – 573Combined sources3
Turni586 – 589Combined sources4
Turni597 – 599Combined sources3
Beta strandi601 – 608Combined sources8
Beta strandi610 – 614Combined sources5
Helixi617 – 624Combined sources8
Helixi626 – 629Combined sources4
Turni630 – 632Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5OJ6X-ray3.30A46-635[»]
5OJKX-ray2.55A/B46-635[»]
ProteinModelPortaliQ8N2Q7.
SMRiQ8N2Q7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1516. Eukaryota.
COG2272. LUCA.
GeneTreeiENSGT00760000118946.
HOGENOMiHOG000231424.
HOVERGENiHBG008839.
InParanoidiQ8N2Q7.
KOiK07378.
OMAiANDFDYA.
OrthoDBiEOG091G0CHW.
PhylomeDBiQ8N2Q7.
TreeFamiTF326187.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiView protein in InterPro
IPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019819. Carboxylesterase_B_CS.
IPR000460. Nlgn.
IPR030022. NLGN1.
PANTHERiPTHR43903:SF2. PTHR43903:SF2. 1 hit.
PfamiView protein in Pfam
PF00135. COesterase. 1 hit.
PRINTSiPR01090. NEUROLIGIN.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiView protein in PROSITE
PS00941. CARBOXYLESTERASE_B_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N2Q7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALPRCTWPN YVWRAVMACL VHRGLGAPLT LCMLGCLLQA GHVLSQKLDD
60 70 80 90 100
VDPLVATNFG KIRGIKKELN NEILGPVIQF LGVPYAAPPT GERRFQPPEP
110 120 130 140 150
PSPWSDIRNA TQFAPVCPQN IIDGRLPEVM LPVWFTNNLD VVSSYVQDQS
160 170 180 190 200
EDCLYLNIYV PTEDGPLTKK RDEATLNPPD TDIRDSGGPK PVMVYIHGGS
210 220 230 240 250
YMEGTGNLYD GSVLASYGNV IVITVNYRLG VLGFLSTGDQ AAKGNYGLLD
260 270 280 290 300
LIQALRWTSE NIGFFGGDPL RITVFGSGAG GSCVNLLTLS HYSEGNRWSN
310 320 330 340 350
STKGLFQRAI AQSGTALSSW AVSFQPAKYA RMLATKVGCN VSDTVELVEC
360 370 380 390 400
LQKKPYKELV DQDIQPARYH IAFGPVIDGD VIPDDPQILM EQGEFLNYDI
410 420 430 440 450
MLGVNQGEGL KFVENIVDSD DGISASDFDF AVSNFVDNLY GYPEGKDVLR
460 470 480 490 500
ETIKFMYTDW ADRHNPETRR KTLLALFTDH QWVAPAVATA DLHSNFGSPT
510 520 530 540 550
YFYAFYHHCQ TDQVPAWADA AHGDEVPYVL GIPMIGPTEL FPCNFSKNDV
560 570 580 590 600
MLSAVVMTYW TNFAKTGDPN QPVPQDTKFI HTKPNRFEEV AWTRYSQKDQ
610 620 630 640 650
LYLHIGLKPR VKEHYRANKV NLWLELVPHL HNLNDISQYT STTTKVPSTD
660 670 680 690 700
ITFRPTRKNS VPVTSAFPTA KQDDPKQQPS PFSVDQRDYS TELSVTIAVG
710 720 730 740 750
ASLLFLNILA FAALYYKKDK RRHDVHRRCS PQRTTTNDLT HAQEEEIMSL
760 770 780 790 800
QMKHTDLDHE CESIHPHEVV LRTACPPDYT LAMRRSPDDV PLMTPNTITM
810 820 830 840
IPNTIPGIQP LHTFNTFTGG QNNTLPHPHP HPHSHSTTRV
Note: No experimental confirmation available.
Length:840
Mass (Da):93,835
Last modified:May 23, 2003 - v2
Checksum:iA3FB21716F62DE29
GO
Isoform 2 (identifier: Q8N2Q7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     165-181: Missing.

Show »
Length:823
Mass (Da):92,000
Checksum:iEA93E8F9801CB8A8
GO

Sequence cautioni

The sequence BAA83022 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAC11039 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti711F → L in BAC11039 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007527165 – 181Missing in isoform 2. 2 PublicationsAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028993 mRNA. Translation: BAA83022.2. Different initiation.
AC008082 Genomic DNA. No translation available.
AC008120 Genomic DNA. No translation available.
AC092923 Genomic DNA. No translation available.
AC092967 Genomic DNA. No translation available.
AC110871 Genomic DNA. No translation available.
AC131158 Genomic DNA. No translation available.
BC032555 mRNA. Translation: AAH32555.1.
AK074522 mRNA. Translation: BAC11039.1. Different initiation.
CCDSiCCDS3222.1. [Q8N2Q7-2]
RefSeqiNP_055747.1. NM_014932.3. [Q8N2Q7-2]
XP_005247291.1. XM_005247234.2. [Q8N2Q7-2]
XP_005247292.1. XM_005247235.3. [Q8N2Q7-2]
XP_016861387.1. XM_017005898.1. [Q8N2Q7-2]
XP_016861388.1. XM_017005899.1. [Q8N2Q7-2]
XP_016861389.1. XM_017005900.1. [Q8N2Q7-2]
XP_016861390.1. XM_017005901.1. [Q8N2Q7-2]
UniGeneiHs.478289.
Hs.732502.

Genome annotation databases

EnsembliENST00000361589; ENSP00000354541; ENSG00000169760. [Q8N2Q7-2]
ENST00000457714; ENSP00000392500; ENSG00000169760. [Q8N2Q7-2]
GeneIDi22871.
KEGGihsa:22871.
UCSCiuc003fio.3. human. [Q8N2Q7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNLGN1_HUMAN
AccessioniPrimary (citable) accession number: Q8N2Q7
Secondary accession number(s): Q9UPT2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: May 23, 2003
Last modified: November 22, 2017
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families