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Q8N2Q7 (NLGN1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuroligin-1
Gene names
Name:NLGN1
Synonyms:KIAA1070
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length840 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses By similarity.

Subunit structure

Interacts with NRXN1, NRXN2 and NRXN3. Interacts with NLGN3. Interacts with AIP1 and PDZRN3 By similarity. Interacts (via its C-terminus) with DLG4/PSD-95 (via PDZ domain 3). Interacts with GOPC. Ref.5 Ref.6

Subcellular location

Cell membrane; Single-pass type I membrane protein By similarity. Cell junctionsynapse By similarity. Cell junctionsynapsepostsynaptic cell membranepostsynaptic density By similarity. Note: Enriched in synaptic plasma membranes and clustered in synaptic clefts and postsynaptic densities. Detected at dendritic spines. Colocalized with DLG4/PSD-95 and GRIN1/NMDAR1 By similarity.

Tissue specificity

Expressed in the blood vessel walls (at protein level). Detected in brain, and at lower levels in pancreas islet beta cells. Ref.7 Ref.8

Sequence similarities

Belongs to the type-B carboxylesterase/lipase family.

Sequence caution

The sequence BAA83022.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence BAC11039.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentCell junction
Cell membrane
Membrane
Postsynaptic cell membrane
Synapse
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processN-methyl-D-aspartate receptor clustering

Inferred from sequence or structural similarity. Source: BHF-UCL

alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor clustering

Inferred from sequence or structural similarity. Source: BHF-UCL

calcium-dependent cell-cell adhesion

Inferred from sequence or structural similarity. Source: UniProtKB

cytoskeletal matrix organization at active zone

Inferred from sequence or structural similarity. Source: BHF-UCL

establishment of protein localization

Inferred from sequence or structural similarity. Source: BHF-UCL

heterophilic cell-cell adhesion

Inferred from sequence or structural similarity. Source: BHF-UCL

nervous system development

Inferred from sequence or structural similarity. Source: UniProtKB

neurexin clustering

Inferred from sequence or structural similarity. Source: BHF-UCL

neuron cell-cell adhesion

Inferred from sequence or structural similarity. Source: BHF-UCL

neuronal signal transduction

Traceable author statement PubMed 15797875. Source: BHF-UCL

positive regulation of dendritic spine development

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of excitatory postsynaptic membrane potential

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of filopodium assembly

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of intracellular signal transduction

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of ruffle assembly

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of synapse assembly

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of synaptic transmission, GABAergic

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of synaptic transmission, glutamatergic

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of synaptic vesicle endocytosis

Inferred from sequence or structural similarity. Source: BHF-UCL

positive regulation of synaptic vesicle exocytosis

Inferred from sequence or structural similarity. Source: BHF-UCL

postsynaptic density protein 95 clustering

Inferred from sequence or structural similarity. Source: BHF-UCL

postsynaptic membrane assembly

Inferred from sequence or structural similarity. Source: BHF-UCL

presynaptic membrane assembly

Inferred from sequence or structural similarity. Source: BHF-UCL

protein heterotetramerization

Inferred from electronic annotation. Source: Ensembl

protein homooligomerization

Inferred from sequence or structural similarity. Source: BHF-UCL

protein localization to synapse

Inferred from sequence or structural similarity. Source: BHF-UCL

protein targeting

Inferred from sequence or structural similarity. Source: UniProtKB

receptor localization to synapse

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of N-methyl-D-aspartate selective glutamate receptor activity

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity

Inferred from sequence or structural similarity. Source: BHF-UCL

regulation of neuron differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of respiratory gaseous exchange by neurological system process

Inferred from electronic annotation. Source: Ensembl

synapse assembly

Inferred from sequence or structural similarity. Source: UniProtKB

synaptic vesicle clustering

Inferred from sequence or structural similarity. Source: BHF-UCL

synaptic vesicle targeting

Inferred from sequence or structural similarity. Source: UniProtKB

terminal button organization

Inferred from sequence or structural similarity. Source: BHF-UCL

   Cellular_componentN-methyl-D-aspartate selective glutamate receptor complex

Inferred from electronic annotation. Source: Ensembl

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

cell surface

Inferred from sequence or structural similarity. Source: BHF-UCL

dendrite

Inferred from sequence or structural similarity. Source: BHF-UCL

external side of plasma membrane

Inferred from electronic annotation. Source: Ensembl

filopodium tip

Inferred from sequence or structural similarity. Source: BHF-UCL

integral component of plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

neuronal postsynaptic density

Traceable author statement PubMed 15797875. Source: BHF-UCL

postsynaptic membrane

Inferred from electronic annotation. Source: UniProtKB-KW

synapse

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionPDZ domain binding

Inferred from direct assay PubMed 17474715. Source: UniProt

cell adhesion molecule binding

Inferred from sequence or structural similarity. Source: BHF-UCL

neurexin family protein binding

Inferred from sequence or structural similarity. Source: UniProtKB

protein dimerization activity

Inferred from sequence or structural similarity. Source: BHF-UCL

receptor activity

Inferred from sequence or structural similarity. Source: BHF-UCL

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8N2Q7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform 2 (identifier: Q8N2Q7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     165-181: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4545 Potential
Chain46 – 840795Neuroligin-1
PRO_0000008640

Regions

Topological domain46 – 694649Extracellular Potential
Transmembrane695 – 71521Helical; Potential
Topological domain716 – 840125Cytoplasmic Potential

Amino acid modifications

Glycosylation1091N-linked (GlcNAc...) (complex) By similarity
Glycosylation3001N-linked (GlcNAc...) (complex) By similarity
Glycosylation3401N-linked (GlcNAc...) (complex) By similarity
Glycosylation5441N-linked (GlcNAc...) By similarity
Glycosylation6801O-linked (GalNAc...) By similarity
Glycosylation6831O-linked (GalNAc...) By similarity
Disulfide bond117 ↔ 153 By similarity
Disulfide bond339 ↔ 350 By similarity
Disulfide bond509 ↔ 543 By similarity

Natural variations

Alternative sequence165 – 18117Missing in isoform 2.
VSP_007527

Experimental info

Sequence conflict7111F → L in BAC11039. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 23, 2003. Version 2.
Checksum: A3FB21716F62DE29

FASTA84093,835
        10         20         30         40         50         60 
MALPRCTWPN YVWRAVMACL VHRGLGAPLT LCMLGCLLQA GHVLSQKLDD VDPLVATNFG 

        70         80         90        100        110        120 
KIRGIKKELN NEILGPVIQF LGVPYAAPPT GERRFQPPEP PSPWSDIRNA TQFAPVCPQN 

       130        140        150        160        170        180 
IIDGRLPEVM LPVWFTNNLD VVSSYVQDQS EDCLYLNIYV PTEDGPLTKK RDEATLNPPD 

       190        200        210        220        230        240 
TDIRDSGGPK PVMVYIHGGS YMEGTGNLYD GSVLASYGNV IVITVNYRLG VLGFLSTGDQ 

       250        260        270        280        290        300 
AAKGNYGLLD LIQALRWTSE NIGFFGGDPL RITVFGSGAG GSCVNLLTLS HYSEGNRWSN 

       310        320        330        340        350        360 
STKGLFQRAI AQSGTALSSW AVSFQPAKYA RMLATKVGCN VSDTVELVEC LQKKPYKELV 

       370        380        390        400        410        420 
DQDIQPARYH IAFGPVIDGD VIPDDPQILM EQGEFLNYDI MLGVNQGEGL KFVENIVDSD 

       430        440        450        460        470        480 
DGISASDFDF AVSNFVDNLY GYPEGKDVLR ETIKFMYTDW ADRHNPETRR KTLLALFTDH 

       490        500        510        520        530        540 
QWVAPAVATA DLHSNFGSPT YFYAFYHHCQ TDQVPAWADA AHGDEVPYVL GIPMIGPTEL 

       550        560        570        580        590        600 
FPCNFSKNDV MLSAVVMTYW TNFAKTGDPN QPVPQDTKFI HTKPNRFEEV AWTRYSQKDQ 

       610        620        630        640        650        660 
LYLHIGLKPR VKEHYRANKV NLWLELVPHL HNLNDISQYT STTTKVPSTD ITFRPTRKNS 

       670        680        690        700        710        720 
VPVTSAFPTA KQDDPKQQPS PFSVDQRDYS TELSVTIAVG ASLLFLNILA FAALYYKKDK 

       730        740        750        760        770        780 
RRHDVHRRCS PQRTTTNDLT HAQEEEIMSL QMKHTDLDHE CESIHPHEVV LRTACPPDYT 

       790        800        810        820        830        840 
LAMRRSPDDV PLMTPNTITM IPNTIPGIQP LHTFNTFTGG QNNTLPHPHP HPHSHSTTRV 

« Hide

Isoform 2 [UniParc].

Checksum: EA93E8F9801CB8A8
Show »

FASTA82392,000

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 6:197-205(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[2]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Duodenum.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 315-823.
Tissue: Embryo.
[5]"Binding of neuroligins to PSD-95."
Irie M., Hata Y., Takeuchi M., Ichtchenko K., Toyoda A., Hirao K., Takai Y., Rosahl T.W., Suedhof T.C.
Science 277:1511-1515(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DLG4.
[6]"Solution structure of GOPC PDZ domain and its interaction with the C-terminal motif of neuroligin."
Li X., Zhang J., Cao Z., Wu J., Shi Y.
Protein Sci. 15:2149-2158(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GOPC.
[7]"Expression of neurexin, neuroligin, and their cytoplasmic binding partners in the pancreatic beta-cells and the involvement of neuroligin in insulin secretion."
Suckow A.T., Comoletti D., Waldrop M.A., Mosedale M., Egodage S., Taylor P., Chessler S.D.
Endocrinology 149:6006-6017(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, ALTERNATIVE SPLICING.
[8]"The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions."
Bottos A., Destro E., Rissone A., Graziano S., Cordara G., Assenzio B., Cera M.R., Mascia L., Bussolino F., Arese M.
Proc. Natl. Acad. Sci. U.S.A. 106:20782-20787(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB028993 mRNA. Translation: BAA83022.2. Different initiation.
AC008082 Genomic DNA. No translation available.
AC008120 Genomic DNA. No translation available.
AC092923 Genomic DNA. No translation available.
AC092967 Genomic DNA. No translation available.
AC110871 Genomic DNA. No translation available.
AC131158 Genomic DNA. No translation available.
BC032555 mRNA. Translation: AAH32555.1.
AK074522 mRNA. Translation: BAC11039.1. Different initiation.
CCDSCCDS3222.1. [Q8N2Q7-2]
RefSeqNP_055747.1. NM_014932.3. [Q8N2Q7-2]
XP_005247291.1. XM_005247234.1. [Q8N2Q7-2]
XP_005247292.1. XM_005247235.1. [Q8N2Q7-2]
UniGeneHs.478289.
Hs.732502.

3D structure databases

ProteinModelPortalQ8N2Q7.
SMRQ8N2Q7. Positions 52-628.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116538. 6 interactions.
IntActQ8N2Q7. 4 interactions.
MINTMINT-1901478.
STRING9606.ENSP00000354541.

Protein family/group databases

MEROPSS09.994.

PTM databases

PhosphoSiteQ8N2Q7.

Polymorphism databases

DMDM31076822.

Proteomic databases

PaxDbQ8N2Q7.
PRIDEQ8N2Q7.

Protocols and materials databases

DNASU22871.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000361589; ENSP00000354541; ENSG00000169760. [Q8N2Q7-2]
ENST00000457714; ENSP00000392500; ENSG00000169760. [Q8N2Q7-2]
ENST00000545397; ENSP00000441108; ENSG00000169760. [Q8N2Q7-2]
GeneID22871.
KEGGhsa:22871.
UCSCuc003fio.1. human. [Q8N2Q7-2]

Organism-specific databases

CTD22871.
GeneCardsGC03P173114.
HGNCHGNC:14291. NLGN1.
HPAHPA006680.
MIM600568. gene.
neXtProtNX_Q8N2Q7.
PharmGKBPA31647.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2272.
HOGENOMHOG000231424.
HOVERGENHBG008839.
InParanoidQ8N2Q7.
KOK07378.
OrthoDBEOG7RBZ7R.
PhylomeDBQ8N2Q7.
TreeFamTF326187.

Gene expression databases

ArrayExpressQ8N2Q7.
BgeeQ8N2Q7.
CleanExHS_NLGN1.
GenevestigatorQ8N2Q7.

Family and domain databases

Gene3D3.40.50.1820. 1 hit.
InterProIPR029058. AB_hydrolase.
IPR002018. CarbesteraseB.
IPR019819. Carboxylesterase_B_CS.
IPR000460. Neuroligin.
[Graphical view]
PfamPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSPR01090. NEUROLIGIN.
SUPFAMSSF53474. SSF53474. 1 hit.
PROSITEPS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNLGN1. human.
GeneWikiNLGN1.
GenomeRNAi22871.
NextBio43403.
PROQ8N2Q7.
SOURCESearch...

Entry information

Entry nameNLGN1_HUMAN
AccessionPrimary (citable) accession number: Q8N2Q7
Secondary accession number(s): Q9UPT2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: May 23, 2003
Last modified: July 9, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM