Reviewed, UniProtKB/Swiss-Prot Q8N2K1 (UB2J2_HUMAN)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 J2
    EC=6.3.2.19
Alternative name(s):
    Non-canonical ubiquitin-conjugating enzyme 2
      Short name=NCUBE2

Gene names

Name:	UBE2J2
Synonyms:	NCUBE2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type IV membrane protein By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Molecular function	Ligase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N2K1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N2K1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: MNSTSSKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEW → MWPQDIAGR

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 259

259

Ubiquitin-conjugating enzyme E2 J2

PRO_0000082596

Regions

Topological domain

1 – 226

226

Cytoplasmic Potential

Transmembrane

227 – 247

21

Anchor for type IV membrane protein Potential

Topological domain

248 – 259

12

Lumenal Potential

Sites

Active site

94

1

Glycyl thioester intermediate By similarity

Natural variations

Alternative sequence

1 – 44

44

MNSTS…NILEW → MWPQDIAGR in isoform 2.

VSP_011572

Experimental info

Sequence conflict

2

1

N → S in AAK52609. Ref.1

Sequence conflict

100

1

F → S in BAC11355. Ref.2

Sequence conflict

214 – 238

25

PNLAG…ANLFV → QTSQGSSRPTGTTDSGWRPG ELVC in AAK52609. Ref.1

Secondary structure

1

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259

Helix Strand Turn

Details...

Helix

13 – 26

14

Beta strand

32 – 37

6

Beta strand

40 – 49

10

Turn

55 – 58

4

Beta strand

60 – 66

7

Turn

69 – 72

4

Beta strand

77 – 80

4

Beta strand

85 – 87

3

Helix

111 – 123

13

Helix

136 – 151

16

Helix

154 – 159

6

Helix

161 – 167

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 13, 2004. Version 2. Checksum: 7DB1023D294C177E	FASTA	259	28,925
	10 20 30 40 50 60 MNSTSSKRAP TTATQRLKQD YLRIKKDPVP YICAEPLPSN ILEWHYVVRG PEMTPYEGGY 70 80 90 100 110 120 YHGKLIFPRE FPFKPPSIYM ITPNGRFKCN TRLCLSITDF HPDTWNPAWS VSTILTGLLS 130 140 150 160 170 180 FMVEKGPTLG SIETSDFTKR QLAVQSLAFN LKDKVFCELF PEVVEEIKQK QKAQDELSSR 190 200 210 220 230 240 PQTLPLPDVV PDGETHLVQN GIQLLNGHAP GAVPNLAGLQ QANRHHGLLG GALANLFVIV 250 GFAAFAYTVK YVLRSIAQE
	Isoform 2. Checksum: D5E649B09D7CC30E	FASTA	224	24,937
	10 20 30 40 50 60 MWPQDIAGRH YVVRGPEMTP YEGGYYHGKL IFPREFPFKP PSIYMITPNG RFKCNTRLCL 70 80 90 100 110 120 SITDFHPDTW NPAWSVSTIL TGLLSFMVEK GPTLGSIETS DFTKRQLAVQ SLAFNLKDKV 130 140 150 160 170 180 FCELFPEVVE EIKQKQKAQD ELSSRPQTLP LPDVVPDGET HLVQNGIQLL NGHAPGAVPN 190 200 210 220 LAGLQQANRH HGLLGGALAN LFVIVGFAAF AYTVKYVLRS IAQE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Endoplasmic reticulum (ER)-associated degradation of T cell receptor subunits. Involvement of ER-associated ubiquitin-conjugating enzymes (E2s)." Tiwari S., Weissman A.M. J. Biol. Chem. 276:16193-16200(2001) [PubMed: 11278356] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Ovary.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF296658 mRNA. Translation: AAK52609.1.
AK056065 mRNA. Translation: BAB71086.1.
AK075017 mRNA. Translation: BAC11355.1.

IPI

IPI00455911.
IPI00873186.

RefSeq

NP_477515.2.
NP_919440.1.

UniGene

Hs.191987

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2F4W	X-ray	2.00	A/B	1-185	[»]

SMR

Q8N2K1. Positions 11-171.

ModBase

Search...

Genome annotation databases

Ensembl

ENSG00000160087. Homo sapiens. [Contig view]

GeneID

118424.

KEGG

hsa:118424.

Organism-specific databases

GeneCards

GC01M001182.

HGNC

HGNC:19268. UBE2J2.

PharmGKB

PA134882268.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

Q8N2K1.

Enzyme and pathway databases

BRENDA

6.3.2.19. 247.

Gene expression databases

ArrayExpress

Q8N2K1.

Bgee

Q8N2K1.

CleanEx

HS_UBE2J2.

GermOnline

ENSG00000160087. Homo sapiens.

Family and domain databases

InterPro

IPR016135. UBQ-conjugat/RWD-like.
IPR000608. UBQ-conjugat_E2.
[Graphical view]

Gene3D

G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.

PANTHER

PTHR11621. UBQ-conjugat_E2. 1 hit.

Pfam

PF00179. UQ_con. 1 hit.
[Graphical view]

ProDom

PD000461. UBQ_conjugat. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00212. UBCc. 1 hit.
[Graphical view]

PROSITE

PS00183. UBIQUITIN_CONJUGAT_1. False negative.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

80253.

Entry information

Entry name

UB2J2_HUMAN

Accession

Primary (citable) accession number: Q8N2K1
Secondary accession number(s): Q96N26, Q96T84

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	September 13, 2004
Last modified:	April 14, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)