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Protein

Monoacylglycerol lipase ABHD12

Gene

ABHD12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lysophosphatidylserine (LPS) lipase that plays a key role in the central nervous system. Represents a major LPS lipase in the brain (By similarity). May also have a 2-arachidonoylglycerol (2-AG) hydrolase activity and act as a regulator of endocannabinoid signaling pathways.By similarity2 Publications

Catalytic activityi

Hydrolyzes glycerol monoesters of long-chain fatty acids.1 Publication

Kineticsi

  1. KM=117 µM for 2-arachidonoyglycerol1 Publication

Vmax=42 nmol/min/mg enzyme toward 2-arachidonoyglycerol1 Publication

pH dependencei

Optimum pH is 7.2-9 with 2-arachidonoyglycerol as substrate.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei246 – 2461Nucleophile1 Publication
Active sitei333 – 3331Charge relay system1 Publication
Active sitei372 – 3721Charge relay system1 Publication

GO - Molecular functioni

  1. acylglycerol lipase activity Source: UniProtKB-EC

GO - Biological processi

  1. adult walking behavior Source: Ensembl
  2. phosphatidylserine catabolic process Source: Ensembl
  3. response to auditory stimulus Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Protein family/group databases

MEROPSiS09.054.

Names & Taxonomyi

Protein namesi
Recommended name:
Monoacylglycerol lipase ABHD12Curated (EC:3.1.1.231 Publication)
Alternative name(s):
2-arachidonoylglycerol hydrolaseCurated
Abhydrolase domain-containing protein 12Curated
Gene namesi
Name:ABHD12Imported
Synonyms:C20orf22Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 20

Organism-specific databases

HGNCiHGNC:15868. ABHD12.

Subcellular locationi

Membrane By similarity; Single-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7474CytoplasmicBy similarityAdd
BLAST
Transmembranei75 – 9521HelicalBy similarityAdd
BLAST
Topological domaini96 – 398303ExtracellularBy similarityAdd
BLAST

GO - Cellular componenti

  1. alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Involvement in diseasei

Polyneuropathy, hearing loss, ataxia, retinitis pigmentosa, and cataract3 Publications

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionA slowly progressive neurologic disorder with a variable phenotype resembling Refsum disease. Clinical features include sensorineural hearing loss, visual problems related to cataracts, retinitis pigmentosa, pes cavus, ataxic and/or spastic gait disturbances with a progressive sensorimotor peripheral neuropathy. Other features include hyporeflexia, hyperreflexia, extensor plantar responses.

See also OMIM:612674

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi246 – 2461S → A: Loss of 2-arachidonoyglycerol hydrolase activity. 1 Publication
Mutagenesisi333 – 3331D → N: Loss of 2-arachidonoyglycerol hydrolase activity. 1 Publication
Mutagenesisi372 – 3721H → A: Loss of 2-arachidonoyglycerol hydrolase activity. 1 Publication

Keywords - Diseasei

Cataract, Deafness, Neuropathy, Retinitis pigmentosa

Organism-specific databases

MIMi612674. phenotype.
Orphaneti171848. Polyneuropathy - hearing loss - ataxia - retinitis pigmentosa - cataract.
PharmGKBiPA25738.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Monoacylglycerol lipase ABHD12PRO_0000079413Add
BLAST

Post-translational modificationi

Glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8N2K0.
PaxDbiQ8N2K0.
PRIDEiQ8N2K0.

PTM databases

PhosphoSiteiQ8N2K0.

Expressioni

Gene expression databases

BgeeiQ8N2K0.
CleanExiHS_ABHD12.
ExpressionAtlasiQ8N2K0. baseline and differential.
GenevestigatoriQ8N2K0.

Organism-specific databases

HPAiHPA026866.

Interactioni

Protein-protein interaction databases

BioGridi117541. 6 interactions.
IntActiQ8N2K0. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ8N2K0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi20 – 256Poly-Ser
Compositional biasi26 – 294Poly-Ala

Sequence similaritiesi

Belongs to the serine esterase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1073.
GeneTreeiENSGT00390000006625.
HOVERGENiHBG051152.
InParanoidiQ8N2K0.
KOiK13704.
OMAiLGVYIAI.
PhylomeDBiQ8N2K0.
TreeFamiTF315122.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR029059. AB_hydrolase_5.
IPR026605. ABHD12.
[Graphical view]
PANTHERiPTHR12277:SF47. PTHR12277:SF47. 1 hit.
PfamiPF12695. Abhydrolase_5. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N2K0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRKRTEPVAL EHERCAAAGS SSSGSAAAAL DADCRLKQNL RLTGPAAAEP
60 70 80 90 100
RCAADAGMKR ALGRRKGVWL RLRKILFCVL GLYIAIPFLI KLCPGIQAKL
110 120 130 140 150
IFLNFVRVPY FIDLKKPQDQ GLNHTCNYYL QPEEDVTIGV WHTVPAVWWK
160 170 180 190 200
NAQGKDQMWY EDALASSHPI ILYLHGNAGT RGGDHRVELY KVLSSLGYHV
210 220 230 240 250
VTFDYRGWGD SVGTPSERGM TYDALHVFDW IKARSGDNPV YIWGHSLGTG
260 270 280 290 300
VATNLVRRLC ERETPPDALI LESPFTNIRE EAKSHPFSVI YRYFPGFDWF
310 320 330 340 350
FLDPITSSGI KFANDENVKH ISCPLLILHA EDDPVVPFQL GRKLYSIAAP
360 370 380 390
ARSFRDFKVQ FVPFHSDLGY RHKYIYKSPE LPRILREFLG KSEPEHQH
Length:398
Mass (Da):45,097
Last modified:November 28, 2003 - v2
Checksum:iE21425C1412B5607
GO
Isoform 2 (identifier: Q8N2K0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     387-398: EFLGKSEPEHQH → PQQGPGSSPDPSMWSELV

Show »
Length:404
Mass (Da):45,558
Checksum:i9BB2A5E05E8BA15F
GO
Isoform 3 (identifier: Q8N2K0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     11-48: Missing.

Show »
Length:360
Mass (Da):41,387
Checksum:i0F9E42F1D556B0E6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti359 – 3591V → I in BAF83504 (PubMed:14702039).Curated
Sequence conflicti376 – 3761Y → C in BAC11357 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti349 – 3491A → T.
Corresponds to variant rs746748 [ dbSNP | Ensembl ].
VAR_050630

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei11 – 4838Missing in isoform 3. 1 PublicationVSP_037372Add
BLAST
Alternative sequencei387 – 39812EFLGK…PEHQH → PQQGPGSSPDPSMWSELV in isoform 2. 1 PublicationVSP_009097Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK075023 mRNA. Translation: BAC11357.1.
AK290815 mRNA. Translation: BAF83504.1.
AK293495 mRNA. Translation: BAG56982.1.
AL121772, AL353812 Genomic DNA. Translation: CAI23474.1.
AL121772, AL353812 Genomic DNA. Translation: CAI23475.1.
AL353812, AL121772 Genomic DNA. Translation: CAI13762.1.
AL353812, AL121772 Genomic DNA. Translation: CAI13763.1.
CH471133 Genomic DNA. Translation: EAX10089.1.
BC014049 mRNA. Translation: AAH14049.1.
AL117442 mRNA. Translation: CAB55927.1.
CCDSiCCDS13172.1. [Q8N2K0-2]
CCDS42857.1. [Q8N2K0-1]
PIRiT17237.
RefSeqiNP_001035937.1. NM_001042472.2. [Q8N2K0-1]
NP_056415.1. NM_015600.4. [Q8N2K0-2]
UniGeneiHs.441550.

Genome annotation databases

EnsembliENST00000339157; ENSP00000341408; ENSG00000100997. [Q8N2K0-1]
ENST00000376542; ENSP00000365725; ENSG00000100997. [Q8N2K0-2]
GeneIDi26090.
KEGGihsa:26090.
UCSCiuc002wuq.3. human. [Q8N2K0-2]
uc002wus.2. human. [Q8N2K0-1]

Polymorphism databases

DMDMi38604894.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK075023 mRNA. Translation: BAC11357.1.
AK290815 mRNA. Translation: BAF83504.1.
AK293495 mRNA. Translation: BAG56982.1.
AL121772, AL353812 Genomic DNA. Translation: CAI23474.1.
AL121772, AL353812 Genomic DNA. Translation: CAI23475.1.
AL353812, AL121772 Genomic DNA. Translation: CAI13762.1.
AL353812, AL121772 Genomic DNA. Translation: CAI13763.1.
CH471133 Genomic DNA. Translation: EAX10089.1.
BC014049 mRNA. Translation: AAH14049.1.
AL117442 mRNA. Translation: CAB55927.1.
CCDSiCCDS13172.1. [Q8N2K0-2]
CCDS42857.1. [Q8N2K0-1]
PIRiT17237.
RefSeqiNP_001035937.1. NM_001042472.2. [Q8N2K0-1]
NP_056415.1. NM_015600.4. [Q8N2K0-2]
UniGeneiHs.441550.

3D structure databases

ProteinModelPortaliQ8N2K0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117541. 6 interactions.
IntActiQ8N2K0. 1 interaction.

Chemistry

BindingDBiQ8N2K0.
ChEMBLiCHEMBL5516.

Protein family/group databases

MEROPSiS09.054.

PTM databases

PhosphoSiteiQ8N2K0.

Polymorphism databases

DMDMi38604894.

Proteomic databases

MaxQBiQ8N2K0.
PaxDbiQ8N2K0.
PRIDEiQ8N2K0.

Protocols and materials databases

DNASUi26090.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000339157; ENSP00000341408; ENSG00000100997. [Q8N2K0-1]
ENST00000376542; ENSP00000365725; ENSG00000100997. [Q8N2K0-2]
GeneIDi26090.
KEGGihsa:26090.
UCSCiuc002wuq.3. human. [Q8N2K0-2]
uc002wus.2. human. [Q8N2K0-1]

Organism-specific databases

CTDi26090.
GeneCardsiGC20M025275.
HGNCiHGNC:15868. ABHD12.
HPAiHPA026866.
MIMi612674. phenotype.
613599. gene.
neXtProtiNX_Q8N2K0.
Orphaneti171848. Polyneuropathy - hearing loss - ataxia - retinitis pigmentosa - cataract.
PharmGKBiPA25738.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1073.
GeneTreeiENSGT00390000006625.
HOVERGENiHBG051152.
InParanoidiQ8N2K0.
KOiK13704.
OMAiLGVYIAI.
PhylomeDBiQ8N2K0.
TreeFamiTF315122.

Miscellaneous databases

ChiTaRSiABHD12. human.
GenomeRNAii26090.
NextBioi48021.
PROiQ8N2K0.
SOURCEiSearch...

Gene expression databases

BgeeiQ8N2K0.
CleanExiHS_ABHD12.
ExpressionAtlasiQ8N2K0. baseline and differential.
GenevestigatoriQ8N2K0.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR029059. AB_hydrolase_5.
IPR026605. ABHD12.
[Graphical view]
PANTHERiPTHR12277:SF47. PTHR12277:SF47. 1 hit.
PfamiPF12695. Abhydrolase_5. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Tissue: Cerebellum, Kidney and Ovarian carcinoma.
  2. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Pancreas.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 152-398 (ISOFORM 1).
    Tissue: Testis.
  6. Cited for: INVOLVEMENT IN PHARC.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Biochemical and pharmacological characterization of human alpha/beta-hydrolase domain containing 6 (ABHD6) and 12 (ABHD12)."
    Navia-Paldanius D., Savinainen J.R., Laitinen J.T.
    J. Lipid Res. 53:2413-2424(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF SER-246; ASP-333 AND HIS-372.
  9. "Targeted next-generation sequencing identifies a homozygous nonsense mutation in ABHD12, the gene underlying PHARC, in a family clinically diagnosed with Usher syndrome type 3."
    Eisenberger T., Slim R., Mansour A., Nauck M., Nurnberg G., Nurnberg P., Decker C., Dafinger C., Ebermann I., Bergmann C., Bolz H.J.
    Orphanet J. Rare Dis. 7:59-59(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INVOLVEMENT IN PHARC.
  10. "Two novel mutations in ABHD12: expansion of the mutation spectrum in PHARC and assessment of their functional effects."
    Chen D.H., Naydenov A., Blankman J.L., Mefford H.C., Davis M., Sul Y., Barloon A.S., Bonkowski E., Wolff J., Matsushita M., Smith C., Cravatt B.F., Mackie K., Raskind W.H., Stella N., Bird T.D.
    Hum. Mutat. 34:1672-1678(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INVOLVEMENT IN PHARC.

Entry informationi

Entry nameiABD12_HUMAN
AccessioniPrimary (citable) accession number: Q8N2K0
Secondary accession number(s): A6NED4
, A6NJ90, A8K450, B4DE71, Q5T710, Q5T711, Q96CR1, Q9BX05, Q9NPX7, Q9UFV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: March 4, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

A family suffering from Polyneuropathy, hearing loss, ataxia, retinitis pigmentosa, and cataract (PHARC) was initially clinically diagnosed with Usher syndrome type 3. Reexamination of one affected member of this family revealed ataxia but not polyneuropathy, demonstrating the phenotypic heterogeneity in PHARC and the need for careful neurological assessments to distinguish this disease from other neuropathic disorders (PubMed:22938382).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.