Q8N2I9 (STK40_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase 40
EC=2.7.11.1

Alternative name(s):
SINK-homologous serine/threonine-protein kinase
Sugen kinase 495
Short name=SgK495

Gene names

Name:	STK40
Synonyms:	SGK495, SHIK

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be a negative regulator of NF-kappa-B and p53-mediated gene transcription. Ref.8
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Nucleus. Cytoplasm Ref.8.
Tissue specificity	Strongly expressed in heart, brain, placenta, lung, skeletal muscle, kidney, spleen, thymus, prostate, liver, pancreas, testis, ovary, small intestine, colon and peripheral blood leukocytes. Ref.8
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Sequence caution	The sequence AAH08344.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB15794.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC11361.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA nucleus Inferred from direct assay. Source: HPA
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N2I9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N2I9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-262: Missing.
     263-295: VLFTMLYGQFPFYDSIPQELFRKIKAAEYTIPE → MGLRQGNGTVSLGILATDPAPPTEPSSPTPCLR

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8N2I9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     364-417: AKVTEECSQY...PPVRRLGHDA → VSWGGQMGHY...GGKGRLQSSA
     418-435: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q8N2I9-4)

The sequence of this isoform differs from the canonical sequence as follows:
     37-37: L → LALCAV

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Serine/threonine-protein kinase 40		PRO_0000252261
Regions
	Domain	35 – 331	297	Protein kinase
	Nucleotide binding	41 – 49	9	ATP By similarity
Sites
	Active site	196	1	Proton acceptor By similarity
	Binding site	66	1	ATP By similarity
Natural variations
	Alternative sequence	1 – 262	262	Missing in isoform 2.		VSP_020896
	Alternative sequence	37	1	L → LALCAV in isoform 4.		VSP_020897
	Alternative sequence	263 – 295	33	VLFTM…YTIPE → MGLRQGNGTVSLGILATDPA PPTEPSSPTPCLR in isoform 2.		VSP_020898
	Alternative sequence	364 – 417	54	AKVTE…LGHDA → VSWGGQMGHYPAPRDRLLGA GRARAEVAATRRPQSFLGLL PQPWGGKGRLQSSA in isoform 3.		VSP_020899
	Alternative sequence	418 – 435	18	Missing in isoform 3.		VSP_020900
	Natural variant	10	1	A → V. Ref.9 Corresponds to variant rs56314546 [ dbSNP | Ensembl ].		VAR_041200
	Natural variant	133	1	M → T in a colorectal adenocarcinoma sample; somatic mutation. Ref.9		VAR_041201
	Natural variant	211	1	R → Q in a colorectal adenocarcinoma sample; somatic mutation. Ref.9		VAR_041202
	Natural variant	395	1	A → T. Ref.2 Ref.6 Ref.9 Corresponds to variant rs3795498 [ dbSNP | Ensembl ].		VAR_041203
Experimental info
	Sequence conflict	191	1	Missing in BAC11371. Ref.2
	Sequence conflict	363	1	E → Q in AAH05169. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: EBA7A19963582BCC	FASTA	435	49,001
	10 20 30 40 50 60 MKRRASDRGA GETSARAKAL GSGISGNNAK RAGPFILGPR LGNSPVPSIV QCLARKDGTD 70 80 90 100 110 120 DFYQLKILTL EERGDQGIES QEERQGKMLL HTEYSLLSLL HTQDGVVHHH GLFQDRTCEI 130 140 150 160 170 180 VEDTESSRMV KKMKKRICLV LDCLCAHDFS DKTADLINLQ HYVIKEKRLS ERETVVIFYD 190 200 210 220 230 240 VVRVVEALHQ KNIVHRDLKL GNMVLNKRTH RITITNFCLG KHLVSEGDLL KDQRGSPAYI 250 260 270 280 290 300 SPDVLSGRPY RGKPSDMWAL GVVLFTMLYG QFPFYDSIPQ ELFRKIKAAE YTIPEDGRVS 310 320 330 340 350 360 ENTVCLIRKL LVLDPQQRLA AADVLEALSA IIASWQSLSS LSGPLQVVPD IDDQMSNADS 370 380 390 400 410 420 SQEAKVTEEC SQYEFENYMR QQLLLAEEKS SIHDARSWVP KRQFGSAPPV RRLGHDAQPM 430 TSLDTAILAQ RYLRK
	Isoform 2 [UniParc]. Checksum: F219891A6F5C9AE1	FASTA	173	18,956
	10 20 30 40 50 60 MGLRQGNGTV SLGILATDPA PPTEPSSPTP CLRDGRVSEN TVCLIRKLLV LDPQQRLAAA 70 80 90 100 110 120 DVLEALSAII ASWQSLSSLS GPLQVVPDID DQMSNADSSQ EAKVTEECSQ YEFENYMRQQ 130 140 150 160 170 LLLAEEKSSI HDARSWVPKR QFGSAPPVRR LGHDAQPMTS LDTAILAQRY LRK
	Isoform 3 [UniParc]. Checksum: 33C6B3B9131B8B77	FASTA	417	46,393
	10 20 30 40 50 60 MKRRASDRGA GETSARAKAL GSGISGNNAK RAGPFILGPR LGNSPVPSIV QCLARKDGTD 70 80 90 100 110 120 DFYQLKILTL EERGDQGIES QEERQGKMLL HTEYSLLSLL HTQDGVVHHH GLFQDRTCEI 130 140 150 160 170 180 VEDTESSRMV KKMKKRICLV LDCLCAHDFS DKTADLINLQ HYVIKEKRLS ERETVVIFYD 190 200 210 220 230 240 VVRVVEALHQ KNIVHRDLKL GNMVLNKRTH RITITNFCLG KHLVSEGDLL KDQRGSPAYI 250 260 270 280 290 300 SPDVLSGRPY RGKPSDMWAL GVVLFTMLYG QFPFYDSIPQ ELFRKIKAAE YTIPEDGRVS 310 320 330 340 350 360 ENTVCLIRKL LVLDPQQRLA AADVLEALSA IIASWQSLSS LSGPLQVVPD IDDQMSNADS 370 380 390 400 410 SQEVSWGGQM GHYPAPRDRL LGAGRARAEV AATRRPQSFL GLLPQPWGGK GRLQSSA
	Isoform 4 [UniParc]. Checksum: 75428BCF0DE3717C	FASTA	440	49,459
	10 20 30 40 50 60 MKRRASDRGA GETSARAKAL GSGISGNNAK RAGPFILALC AVGPRLGNSP VPSIVQCLAR 70 80 90 100 110 120 KDGTDDFYQL KILTLEERGD QGIESQEERQ GKMLLHTEYS LLSLLHTQDG VVHHHGLFQD 130 140 150 160 170 180 RTCEIVEDTE SSRMVKKMKK RICLVLDCLC AHDFSDKTAD LINLQHYVIK EKRLSERETV 190 200 210 220 230 240 VIFYDVVRVV EALHQKNIVH RDLKLGNMVL NKRTHRITIT NFCLGKHLVS EGDLLKDQRG 250 260 270 280 290 300 SPAYISPDVL SGRPYRGKPS DMWALGVVLF TMLYGQFPFY DSIPQELFRK IKAAEYTIPE 310 320 330 340 350 360 DGRVSENTVC LIRKLLVLDP QQRLAAADVL EALSAIIASW QSLSSLSGPL QVVPDIDDQM 370 380 390 400 410 420 SNADSSQEAK VTEECSQYEF ENYMRQQLLL AEEKSSIHDA RSWVPKRQFG SAPPVRRLGH 430 440 DAQPMTSLDT AILAQRYLRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a novel Ser/Thr-like kinase." Shan Y.X., Wu H., Yu L. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3), VARIANT THR-395. Tissue: Ovary, Spleen and Testis.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Melanoma.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4), VARIANT THR-395. Tissue: Kidney and Muscle.
[7]	"The protein kinase complement of the human genome." Manning G., Whyte D.B., Martinez R., Hunter T., Sudarsanam S. Science 298:1912-1934(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[8]	"Identification of a novel serine/threonine kinase that inhibits TNF-induced NF-kappaB activation and p53-induced transcription." Huang J., Teng L., Liu T., Li L., Chen D., Li F., Xu L.-G., Zhai Z., Shu H.-B. Biochem. Biophys. Res. Commun. 309:774-778(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[9]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] VAL-10; THR-133; GLN-211 AND THR-395.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY198395 mRNA. Translation: AAP20040.1. AK024504 mRNA. Translation: BAB15794.1. Different initiation. AK075029 mRNA. Translation: BAC11361.1. Different initiation. AK075048 mRNA. Translation: BAC11371.1. AK131560 mRNA. Translation: BAD18694.1. AL834137 mRNA. Translation: CAD38852.1. AL591845 Genomic DNA. Translation: CAH71862.1. AL591845 Genomic DNA. Translation: CAH71863.1. AL591845 Genomic DNA. Translation: CAH71864.1. CH471059 Genomic DNA. Translation: EAX07371.1. CH471059 Genomic DNA. Translation: EAX07373.1. BC005169 mRNA. Translation: AAH05169.3. BC007835 mRNA. Translation: AAH07835.2. BC008344 mRNA. Translation: AAH08344.1. Different initiation.
IPI	IPI00161013. IPI00181701. IPI00644404. IPI00790155.
RefSeq	NP_114406.1. NM_032017.1.
UniGene	Hs.471768.
3D structure databases
ProteinModelPortal	Q8N2I9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8N2I9. 2 interactions.
MINT	MINT-1382789.
Polymorphism databases
DMDM	116256080.
Proteomic databases
PaxDb	Q8N2I9.
PRIDE	Q8N2I9.
Protocols and materials databases
DNASU	83931.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000359297; ENSP00000352245; ENSG00000196182. ENST00000373129; ENSP00000362221; ENSG00000196182. ENST00000373130; ENSP00000362222; ENSG00000196182. ENST00000373132; ENSP00000362224; ENSG00000196182.
GeneID	83931.
KEGG	hsa:83931.
UCSC	uc001cak.1. human. uc001cal.1. human. uc001can.1. human.
Organism-specific databases
CTD	83931.
GeneCards	GC01M036805.
HGNC	HGNC:21373. STK40.
HPA	HPA008026.
MIM	609437. gene.
neXtProt	NX_Q8N2I9.
PharmGKB	PA142670860.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0515.
HOVERGEN	HBG080296.
KO	K16312.
OMA	SSIIASW.
OrthoDB	EOG4ZPDV8.
PhylomeDB	Q8N2I9.
Gene expression databases
Bgee	Q8N2I9.
Genevestigator	Q8N2I9.
GermOnline	ENSG00000196182. Homo sapiens.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR024236. Ser/Thr_kinase_40. IPR008271. Ser/Thr_kinase_AS. IPR024104. Tribbles/Ser_Thr_kinase_40. [Graphical view]
PANTHER	PTHR22961. PTHR22961. 1 hit. PTHR22961:SF1. PTHR22961:SF1. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	STK40. human.
GenomeRNAi	83931.
NextBio	73043.
SOURCE	Search...

Entry information

Entry name

STK40_HUMAN

Accession

Primary (citable) accession number: Q8N2I9
Secondary accession number(s): D3DPS8 , Q5VTK8, Q5VTK9, Q6ZMN1, Q8N2J8, Q8N3I6, Q96HN6, Q96I44, Q9BSA3, Q9H7H6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.