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Q8N2H9

- PELI3_HUMAN

UniProt

Q8N2H9 - PELI3_HUMAN

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Protein

E3 ubiquitin-protein ligase pellino homolog 3

Gene

PELI3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1. Can activate AP1/JUN and ELK1. Not required for NF-kappa-B activation.2 Publications

Pathwayi

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. ubiquitin-protein transferase activity Source: Ensembl

GO - Biological processi

  1. defense response to Gram-negative bacterium Source: Ensembl
  2. negative regulation of extrinsic apoptotic signaling pathway Source: MGI
  3. negative regulation of protein ubiquitination Source: Ensembl
  4. negative regulation of Toll signaling pathway Source: Ensembl
  5. negative regulation of tumor necrosis factor-mediated signaling pathway Source: MGI
  6. negative regulation of type I interferon production Source: Ensembl
  7. positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway Source: Ensembl
  8. protein K63-linked ubiquitination Source: Ensembl
  9. regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway Source: Ensembl
  10. Toll signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_22442. Interleukin-1 signaling.
REACT_24918. IRAK1 recruits IKK complex.
REACT_25354. IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase pellino homolog 3 (EC:6.3.2.-)
Short name:
Pellino-3
Gene namesi
Name:PELI3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:30010. PELI3.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671185.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469E3 ubiquitin-protein ligase pellino homolog 3PRO_0000194176Add
BLAST

Post-translational modificationi

Phosphorylated by IRAK1 enhancing its E3 ligase activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8N2H9.
PRIDEiQ8N2H9.

PTM databases

PhosphoSiteiQ8N2H9.

Expressioni

Tissue specificityi

Highly expressed in brain, heart and testis, and at lower level in kidney, liver, lung, placenta, small intestine, spleen and stomach. Isoform 1 is not expressed in lung.1 Publication

Gene expression databases

BgeeiQ8N2H9.
CleanExiHS_PELI3.
ExpressionAtlasiQ8N2H9. baseline and differential.
GenevestigatoriQ8N2H9.

Organism-specific databases

HPAiCAB033750.

Interactioni

Subunit structurei

Interacts with TRAF6, IRAK1, MAP3K14 and MAP3K7.1 Publication

Protein-protein interaction databases

BioGridi128900. 14 interactions.
DIPiDIP-31770N.
IntActiQ8N2H9. 4 interactions.
MINTiMINT-2810142.
STRINGi9606.ENSP00000322532.

Structurei

3D structure databases

ProteinModelPortaliQ8N2H9.
SMRiQ8N2H9. Positions 40-305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pellino family.Curated

Phylogenomic databases

eggNOGiNOG258040.
GeneTreeiENSGT00390000007680.
HOGENOMiHOG000234110.
HOVERGENiHBG053559.
InParanoidiQ8N2H9.
KOiK11964.
OMAiTSDLQHP.
OrthoDBiEOG751NFB.
PhylomeDBiQ8N2H9.
TreeFamiTF314338.

Family and domain databases

InterProiIPR006800. Pellino_fam.
[Graphical view]
PANTHERiPTHR12098. PTHR12098. 1 hit.
PfamiPF04710. Pellino. 1 hit.
[Graphical view]
PIRSFiPIRSF038886. Pellino. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8N2H9-1) [UniParc]FASTAAdd to Basket

Also known as: Alpha, A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLEGNPEVG SPRTSDLQHR GNKGSCVLSS PGEDAQPGEE PIKYGELIVL
60 70 80 90 100
GCCEEGGEET EAQRGEVTGP RAHSCYNGCL ASGDKGRRRS RLALSRRSHA
110 120 130 140 150
NGVKPDVMHH ISTPLVSKAL SNRGQHSISY TLSRSHSVIV EYTHDSDTDM
160 170 180 190 200
FQIGRSTENM IDFVVTDTSP GGGAAEGPSA QSTISRYACR ILCDRRPPYT
210 220 230 240 250
ARIYAAGFDA SSNIFLGERA AKWRTPDGLM DGLTTNGVLV MHPAGGFSED
260 270 280 290 300
SAPGVWREIS VCGNVYTLRD SRSAQQRGKL VENESNVLQD GSLIDLCGAT
310 320 330 340 350
LLWRTPAGLL RAPTLKQLEA QRQEANAARP QCPVGLSTLA FPSPARGRTA
360 370 380 390 400
PDKQQPWVYV RCGHVHGYHG WGCRRERGPQ ERECPLCRLV GPYVPLWLGQ
410 420 430 440 450
EAGLCLDPGP PSHAFAPCGH VCSEKTARYW AQTPLPHGTH AFHAACPFCG
460
AWLTGEHGCV RLIFQGPLD
Length:469
Mass (Da):50,755
Last modified:October 24, 2003 - v2
Checksum:i7E14D1405B023F96
GO
Isoform 2 (identifier: Q8N2H9-2) [UniParc]FASTAAdd to Basket

Also known as: Beta, B

The sequence of this isoform differs from the canonical sequence as follows:
     52-75: Missing.

Show »
Length:445
Mass (Da):48,237
Checksum:iAD1D7E00533CD807
GO
Isoform 3 (identifier: Q8N2H9-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     48-110: Missing.

Note: No experimental confirmation available.

Show »
Length:406
Mass (Da):43,970
Checksum:iEDFD5137B164C41E
GO
Isoform 4 (identifier: Q8N2H9-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     282-331: ENESNVLQDG...RQEANAARPQ → GGPLHSPPLS...YQGIPGPGGS
     332-469: Missing.

Note: No experimental confirmation available.

Show »
Length:331
Mass (Da):35,339
Checksum:iF0B85D8FF892B233
GO

Sequence cautioni

The sequence BAC11499.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731H → L in BAB85015. (PubMed:14702039)Curated
Sequence conflicti109 – 1091H → L in BAB85015. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti287 – 2871V → M.
Corresponds to variant rs34989499 [ dbSNP | Ensembl ].
VAR_061502

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei48 – 11063Missing in isoform 3. 1 PublicationVSP_008639Add
BLAST
Alternative sequencei52 – 7524Missing in isoform 2. 2 PublicationsVSP_008640Add
BLAST
Alternative sequencei282 – 33150ENESN…AARPQ → GGPLHSPPLSLPGPHKLPIP LPKPGDDLQSFCPREPQQAP YQGIPGPGGS in isoform 4. 1 PublicationVSP_008641Add
BLAST
Alternative sequencei332 – 469138Missing in isoform 4. 1 PublicationVSP_008642Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF487456 mRNA. Translation: AAO49465.1.
AF487457 mRNA. Translation: AAO49466.1.
AK074201 mRNA. Translation: BAB85015.1.
AK075253 mRNA. Translation: BAC11499.1. Different initiation.
AK094060 mRNA. Translation: BAC04275.1.
AL834395 mRNA. Translation: CAD39057.1.
BC021256 mRNA. Translation: AAH21256.1.
BC025723 mRNA. Translation: AAH25723.1.
CCDSiCCDS31615.1. [Q8N2H9-1]
CCDS41675.1. [Q8N2H9-2]
CCDS73328.1. [Q8N2H9-3]
RefSeqiNP_001091980.1. NM_001098510.1. [Q8N2H9-2]
NP_001230064.1. NM_001243135.1. [Q8N2H9-3]
NP_001230065.1. NM_001243136.1.
NP_659502.2. NM_145065.2. [Q8N2H9-1]
XP_006718547.1. XM_006718484.1. [Q8N2H9-2]
UniGeneiHs.656875.

Genome annotation databases

EnsembliENST00000320740; ENSP00000322532; ENSG00000174516. [Q8N2H9-1]
ENST00000349459; ENSP00000309848; ENSG00000174516. [Q8N2H9-2]
ENST00000524466; ENSP00000434677; ENSG00000174516. [Q8N2H9-4]
ENST00000618547; ENSP00000484220; ENSG00000174516. [Q8N2H9-3]
GeneIDi246330.
KEGGihsa:246330.
UCSCiuc001oib.2. human. [Q8N2H9-4]
uc001oic.4. human. [Q8N2H9-1]
uc001oid.4. human. [Q8N2H9-2]
uc021qlx.1. human. [Q8N2H9-3]

Polymorphism databases

DMDMi37999712.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF487456 mRNA. Translation: AAO49465.1 .
AF487457 mRNA. Translation: AAO49466.1 .
AK074201 mRNA. Translation: BAB85015.1 .
AK075253 mRNA. Translation: BAC11499.1 . Different initiation.
AK094060 mRNA. Translation: BAC04275.1 .
AL834395 mRNA. Translation: CAD39057.1 .
BC021256 mRNA. Translation: AAH21256.1 .
BC025723 mRNA. Translation: AAH25723.1 .
CCDSi CCDS31615.1. [Q8N2H9-1 ]
CCDS41675.1. [Q8N2H9-2 ]
CCDS73328.1. [Q8N2H9-3 ]
RefSeqi NP_001091980.1. NM_001098510.1. [Q8N2H9-2 ]
NP_001230064.1. NM_001243135.1. [Q8N2H9-3 ]
NP_001230065.1. NM_001243136.1.
NP_659502.2. NM_145065.2. [Q8N2H9-1 ]
XP_006718547.1. XM_006718484.1. [Q8N2H9-2 ]
UniGenei Hs.656875.

3D structure databases

ProteinModelPortali Q8N2H9.
SMRi Q8N2H9. Positions 40-305.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 128900. 14 interactions.
DIPi DIP-31770N.
IntActi Q8N2H9. 4 interactions.
MINTi MINT-2810142.
STRINGi 9606.ENSP00000322532.

PTM databases

PhosphoSitei Q8N2H9.

Polymorphism databases

DMDMi 37999712.

Proteomic databases

PaxDbi Q8N2H9.
PRIDEi Q8N2H9.

Protocols and materials databases

DNASUi 246330.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000320740 ; ENSP00000322532 ; ENSG00000174516 . [Q8N2H9-1 ]
ENST00000349459 ; ENSP00000309848 ; ENSG00000174516 . [Q8N2H9-2 ]
ENST00000524466 ; ENSP00000434677 ; ENSG00000174516 . [Q8N2H9-4 ]
ENST00000618547 ; ENSP00000484220 ; ENSG00000174516 . [Q8N2H9-3 ]
GeneIDi 246330.
KEGGi hsa:246330.
UCSCi uc001oib.2. human. [Q8N2H9-4 ]
uc001oic.4. human. [Q8N2H9-1 ]
uc001oid.4. human. [Q8N2H9-2 ]
uc021qlx.1. human. [Q8N2H9-3 ]

Organism-specific databases

CTDi 246330.
GeneCardsi GC11P066234.
HGNCi HGNC:30010. PELI3.
HPAi CAB033750.
MIMi 609827. gene.
neXtProti NX_Q8N2H9.
PharmGKBi PA142671185.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG258040.
GeneTreei ENSGT00390000007680.
HOGENOMi HOG000234110.
HOVERGENi HBG053559.
InParanoidi Q8N2H9.
KOi K11964.
OMAi TSDLQHP.
OrthoDBi EOG751NFB.
PhylomeDBi Q8N2H9.
TreeFami TF314338.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_22442. Interleukin-1 signaling.
REACT_24918. IRAK1 recruits IKK complex.
REACT_25354. IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation.

Miscellaneous databases

ChiTaRSi PELI3. human.
GenomeRNAii 246330.
NextBioi 91928.
PROi Q8N2H9.
SOURCEi Search...

Gene expression databases

Bgeei Q8N2H9.
CleanExi HS_PELI3.
ExpressionAtlasi Q8N2H9. baseline and differential.
Genevestigatori Q8N2H9.

Family and domain databases

InterProi IPR006800. Pellino_fam.
[Graphical view ]
PANTHERi PTHR12098. PTHR12098. 1 hit.
Pfami PF04710. Pellino. 1 hit.
[Graphical view ]
PIRSFi PIRSF038886. Pellino. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Pellino3, a novel member of the Pellino protein family, promotes activation of c-Jun and Elk-1 and may act as a scaffolding protein."
    Jensen L.E., Whitehead A.S.
    J. Immunol. 171:1500-1506(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH IRAK1; TRAF6; MAP3K7 AND MAP3K14.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Tissue: Adipose tissue, Thyroid and Uterus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Amygdala.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
    Tissue: Brain and Lung.
  5. "The IRAK-catalysed activation of the E3 ligase function of Pellino isoforms induces the Lys63-linked polyubiquitination of IRAK1."
    Ordureau A., Smith H., Windheim M., Peggie M., Carrick E., Morrice N., Cohen P.
    Biochem. J. 409:43-52(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY IRAK1.
  6. "Kinase-active interleukin-1 receptor-associated kinases promote polyubiquitination and degradation of the Pellino family: direct evidence for PELLINO proteins being ubiquitin-protein isopeptide ligases."
    Butler M.P., Hanly J.A., Moynagh P.N.
    J. Biol. Chem. 282:29729-29737(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPELI3_HUMAN
AccessioniPrimary (citable) accession number: Q8N2H9
Secondary accession number(s): Q8N3E1
, Q8N9Q6, Q8TAW7, Q8TED5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: October 24, 2003
Last modified: October 29, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3