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Q8N2H9

- PELI3_HUMAN

UniProt

Q8N2H9 - PELI3_HUMAN

Protein

E3 ubiquitin-protein ligase pellino homolog 3

Gene

PELI3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (24 Oct 2003)
      Previous versions | rss
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    Functioni

    E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1. Can activate AP1/JUN and ELK1. Not required for NF-kappa-B activation.2 Publications

    Pathwayi

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW

    GO - Biological processi

    1. negative regulation of extrinsic apoptotic signaling pathway Source: MGI
    2. negative regulation of tumor necrosis factor-mediated signaling pathway Source: MGI
    3. protein ubiquitination Source: UniProtKB-UniPathway
    4. Toll signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    ReactomeiREACT_22442. Interleukin-1 signaling.
    REACT_24918. IRAK1 recruits IKK complex.
    REACT_25354. IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 ubiquitin-protein ligase pellino homolog 3 (EC:6.3.2.-)
    Short name:
    Pellino-3
    Gene namesi
    Name:PELI3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:30010. PELI3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142671185.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 469469E3 ubiquitin-protein ligase pellino homolog 3PRO_0000194176Add
    BLAST

    Post-translational modificationi

    Phosphorylated by IRAK1 enhancing its E3 ligase activity.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8N2H9.
    PRIDEiQ8N2H9.

    PTM databases

    PhosphoSiteiQ8N2H9.

    Expressioni

    Tissue specificityi

    Highly expressed in brain, heart and testis, and at lower level in kidney, liver, lung, placenta, small intestine, spleen and stomach. Isoform 1 is not expressed in lung.1 Publication

    Gene expression databases

    ArrayExpressiQ8N2H9.
    BgeeiQ8N2H9.
    CleanExiHS_PELI3.
    GenevestigatoriQ8N2H9.

    Organism-specific databases

    HPAiCAB033750.

    Interactioni

    Subunit structurei

    Interacts with TRAF6, IRAK1, MAP3K14 and MAP3K7.1 Publication

    Protein-protein interaction databases

    BioGridi128900. 11 interactions.
    DIPiDIP-31770N.
    IntActiQ8N2H9. 4 interactions.
    MINTiMINT-2810142.
    STRINGi9606.ENSP00000322532.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8N2H9.
    SMRiQ8N2H9. Positions 40-305.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pellino family.Curated

    Phylogenomic databases

    eggNOGiNOG258040.
    HOGENOMiHOG000234110.
    HOVERGENiHBG053559.
    InParanoidiQ8N2H9.
    KOiK11964.
    OMAiTSDLQHP.
    OrthoDBiEOG751NFB.
    PhylomeDBiQ8N2H9.
    TreeFamiTF314338.

    Family and domain databases

    InterProiIPR006800. Pellino_fam.
    [Graphical view]
    PANTHERiPTHR12098. PTHR12098. 1 hit.
    PfamiPF04710. Pellino. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038886. Pellino. 1 hit.

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8N2H9-1) [UniParc]FASTAAdd to Basket

    Also known as: Alpha, A

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVLEGNPEVG SPRTSDLQHR GNKGSCVLSS PGEDAQPGEE PIKYGELIVL    50
    GCCEEGGEET EAQRGEVTGP RAHSCYNGCL ASGDKGRRRS RLALSRRSHA 100
    NGVKPDVMHH ISTPLVSKAL SNRGQHSISY TLSRSHSVIV EYTHDSDTDM 150
    FQIGRSTENM IDFVVTDTSP GGGAAEGPSA QSTISRYACR ILCDRRPPYT 200
    ARIYAAGFDA SSNIFLGERA AKWRTPDGLM DGLTTNGVLV MHPAGGFSED 250
    SAPGVWREIS VCGNVYTLRD SRSAQQRGKL VENESNVLQD GSLIDLCGAT 300
    LLWRTPAGLL RAPTLKQLEA QRQEANAARP QCPVGLSTLA FPSPARGRTA 350
    PDKQQPWVYV RCGHVHGYHG WGCRRERGPQ ERECPLCRLV GPYVPLWLGQ 400
    EAGLCLDPGP PSHAFAPCGH VCSEKTARYW AQTPLPHGTH AFHAACPFCG 450
    AWLTGEHGCV RLIFQGPLD 469
    Length:469
    Mass (Da):50,755
    Last modified:October 24, 2003 - v2
    Checksum:i7E14D1405B023F96
    GO
    Isoform 2 (identifier: Q8N2H9-2) [UniParc]FASTAAdd to Basket

    Also known as: Beta, B

    The sequence of this isoform differs from the canonical sequence as follows:
         52-75: Missing.

    Show »
    Length:445
    Mass (Da):48,237
    Checksum:iAD1D7E00533CD807
    GO
    Isoform 3 (identifier: Q8N2H9-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         48-110: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:406
    Mass (Da):43,970
    Checksum:iEDFD5137B164C41E
    GO
    Isoform 4 (identifier: Q8N2H9-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         282-331: ENESNVLQDG...RQEANAARPQ → GGPLHSPPLS...YQGIPGPGGS
         332-469: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:331
    Mass (Da):35,339
    Checksum:iF0B85D8FF892B233
    GO

    Sequence cautioni

    The sequence BAC11499.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti73 – 731H → L in BAB85015. (PubMed:14702039)Curated
    Sequence conflicti109 – 1091H → L in BAB85015. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti287 – 2871V → M.
    Corresponds to variant rs34989499 [ dbSNP | Ensembl ].
    VAR_061502

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei48 – 11063Missing in isoform 3. 1 PublicationVSP_008639Add
    BLAST
    Alternative sequencei52 – 7524Missing in isoform 2. 2 PublicationsVSP_008640Add
    BLAST
    Alternative sequencei282 – 33150ENESN…AARPQ → GGPLHSPPLSLPGPHKLPIP LPKPGDDLQSFCPREPQQAP YQGIPGPGGS in isoform 4. 1 PublicationVSP_008641Add
    BLAST
    Alternative sequencei332 – 469138Missing in isoform 4. 1 PublicationVSP_008642Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF487456 mRNA. Translation: AAO49465.1.
    AF487457 mRNA. Translation: AAO49466.1.
    AK074201 mRNA. Translation: BAB85015.1.
    AK075253 mRNA. Translation: BAC11499.1. Different initiation.
    AK094060 mRNA. Translation: BAC04275.1.
    AL834395 mRNA. Translation: CAD39057.1.
    BC021256 mRNA. Translation: AAH21256.1.
    BC025723 mRNA. Translation: AAH25723.1.
    CCDSiCCDS31615.1. [Q8N2H9-1]
    CCDS41675.1. [Q8N2H9-2]
    RefSeqiNP_001091980.1. NM_001098510.1. [Q8N2H9-2]
    NP_001230064.1. NM_001243135.1. [Q8N2H9-3]
    NP_001230065.1. NM_001243136.1.
    NP_659502.2. NM_145065.2. [Q8N2H9-1]
    XP_006718547.1. XM_006718484.1. [Q8N2H9-2]
    UniGeneiHs.656875.

    Genome annotation databases

    EnsembliENST00000320740; ENSP00000322532; ENSG00000174516. [Q8N2H9-1]
    ENST00000349459; ENSP00000309848; ENSG00000174516. [Q8N2H9-2]
    ENST00000524466; ENSP00000434677; ENSG00000174516. [Q8N2H9-4]
    GeneIDi246330.
    KEGGihsa:246330.
    UCSCiuc001oib.2. human. [Q8N2H9-4]
    uc001oic.4. human. [Q8N2H9-1]
    uc001oid.4. human. [Q8N2H9-2]
    uc021qlx.1. human. [Q8N2H9-3]

    Polymorphism databases

    DMDMi37999712.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF487456 mRNA. Translation: AAO49465.1 .
    AF487457 mRNA. Translation: AAO49466.1 .
    AK074201 mRNA. Translation: BAB85015.1 .
    AK075253 mRNA. Translation: BAC11499.1 . Different initiation.
    AK094060 mRNA. Translation: BAC04275.1 .
    AL834395 mRNA. Translation: CAD39057.1 .
    BC021256 mRNA. Translation: AAH21256.1 .
    BC025723 mRNA. Translation: AAH25723.1 .
    CCDSi CCDS31615.1. [Q8N2H9-1 ]
    CCDS41675.1. [Q8N2H9-2 ]
    RefSeqi NP_001091980.1. NM_001098510.1. [Q8N2H9-2 ]
    NP_001230064.1. NM_001243135.1. [Q8N2H9-3 ]
    NP_001230065.1. NM_001243136.1.
    NP_659502.2. NM_145065.2. [Q8N2H9-1 ]
    XP_006718547.1. XM_006718484.1. [Q8N2H9-2 ]
    UniGenei Hs.656875.

    3D structure databases

    ProteinModelPortali Q8N2H9.
    SMRi Q8N2H9. Positions 40-305.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 128900. 11 interactions.
    DIPi DIP-31770N.
    IntActi Q8N2H9. 4 interactions.
    MINTi MINT-2810142.
    STRINGi 9606.ENSP00000322532.

    PTM databases

    PhosphoSitei Q8N2H9.

    Polymorphism databases

    DMDMi 37999712.

    Proteomic databases

    PaxDbi Q8N2H9.
    PRIDEi Q8N2H9.

    Protocols and materials databases

    DNASUi 246330.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000320740 ; ENSP00000322532 ; ENSG00000174516 . [Q8N2H9-1 ]
    ENST00000349459 ; ENSP00000309848 ; ENSG00000174516 . [Q8N2H9-2 ]
    ENST00000524466 ; ENSP00000434677 ; ENSG00000174516 . [Q8N2H9-4 ]
    GeneIDi 246330.
    KEGGi hsa:246330.
    UCSCi uc001oib.2. human. [Q8N2H9-4 ]
    uc001oic.4. human. [Q8N2H9-1 ]
    uc001oid.4. human. [Q8N2H9-2 ]
    uc021qlx.1. human. [Q8N2H9-3 ]

    Organism-specific databases

    CTDi 246330.
    GeneCardsi GC11P066234.
    HGNCi HGNC:30010. PELI3.
    HPAi CAB033750.
    MIMi 609827. gene.
    neXtProti NX_Q8N2H9.
    PharmGKBi PA142671185.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG258040.
    HOGENOMi HOG000234110.
    HOVERGENi HBG053559.
    InParanoidi Q8N2H9.
    KOi K11964.
    OMAi TSDLQHP.
    OrthoDBi EOG751NFB.
    PhylomeDBi Q8N2H9.
    TreeFami TF314338.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_22442. Interleukin-1 signaling.
    REACT_24918. IRAK1 recruits IKK complex.
    REACT_25354. IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation.

    Miscellaneous databases

    ChiTaRSi PELI3. human.
    GenomeRNAii 246330.
    NextBioi 91928.
    PROi Q8N2H9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8N2H9.
    Bgeei Q8N2H9.
    CleanExi HS_PELI3.
    Genevestigatori Q8N2H9.

    Family and domain databases

    InterProi IPR006800. Pellino_fam.
    [Graphical view ]
    PANTHERi PTHR12098. PTHR12098. 1 hit.
    Pfami PF04710. Pellino. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038886. Pellino. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Pellino3, a novel member of the Pellino protein family, promotes activation of c-Jun and Elk-1 and may act as a scaffolding protein."
      Jensen L.E., Whitehead A.S.
      J. Immunol. 171:1500-1506(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH IRAK1; TRAF6; MAP3K7 AND MAP3K14.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
      Tissue: Adipose tissue, Thyroid and Uterus.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Amygdala.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
      Tissue: Brain and Lung.
    5. "The IRAK-catalysed activation of the E3 ligase function of Pellino isoforms induces the Lys63-linked polyubiquitination of IRAK1."
      Ordureau A., Smith H., Windheim M., Peggie M., Carrick E., Morrice N., Cohen P.
      Biochem. J. 409:43-52(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION BY IRAK1.
    6. "Kinase-active interleukin-1 receptor-associated kinases promote polyubiquitination and degradation of the Pellino family: direct evidence for PELLINO proteins being ubiquitin-protein isopeptide ligases."
      Butler M.P., Hanly J.A., Moynagh P.N.
      J. Biol. Chem. 282:29729-29737(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPELI3_HUMAN
    AccessioniPrimary (citable) accession number: Q8N2H9
    Secondary accession number(s): Q8N3E1
    , Q8N9Q6, Q8TAW7, Q8TED5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 24, 2003
    Last sequence update: October 24, 2003
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3