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Q8N2H9 (PELI3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
E3 ubiquitin-protein ligase pellino homolog 3

Short name=Pellino-3
EC=6.3.2.-
Gene names
Name:PELI3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1. Can activate AP1/JUN and ELK1. Not required for NF-kappa-B activation. Ref.1 Ref.6

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with TRAF6, IRAK1, MAP3K14 and MAP3K7. Ref.1

Tissue specificity

Highly expressed in brain, heart and testis, and at lower level in kidney, liver, lung, placenta, small intestine, spleen and stomach. Isoform 1 is not expressed in lung. Ref.1

Post-translational modification

Phosphorylated by IRAK1 enhancing its E3 ligase activity. Ref.5

Sequence similarities

Belongs to the pellino family.

Sequence caution

The sequence BAC11499.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Coding sequence diversityAlternative splicing
Polymorphism
   Molecular functionLigase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processToll signaling pathway

Inferred from electronic annotation. Source: InterPro

protein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytosol

Traceable author statement. Source: Reactome

   Molecular_functionligase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8N2H9-1)

Also known as: Alpha; A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8N2H9-2)

Also known as: Beta; B;

The sequence of this isoform differs from the canonical sequence as follows:
     52-75: Missing.
Isoform 3 (identifier: Q8N2H9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     48-110: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q8N2H9-4)

The sequence of this isoform differs from the canonical sequence as follows:
     282-331: ENESNVLQDG...RQEANAARPQ → GGPLHSPPLS...YQGIPGPGGS
     332-469: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 469469E3 ubiquitin-protein ligase pellino homolog 3
PRO_0000194176

Natural variations

Alternative sequence48 – 11063Missing in isoform 3.
VSP_008639
Alternative sequence52 – 7524Missing in isoform 2.
VSP_008640
Alternative sequence282 – 33150ENESN…AARPQ → GGPLHSPPLSLPGPHKLPIP LPKPGDDLQSFCPREPQQAP YQGIPGPGGS in isoform 4.
VSP_008641
Alternative sequence332 – 469138Missing in isoform 4.
VSP_008642
Natural variant2871V → M.
Corresponds to variant rs34989499 [ dbSNP | Ensembl ].
VAR_061502

Experimental info

Sequence conflict731H → L in BAB85015. Ref.2
Sequence conflict1091H → L in BAB85015. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Alpha) (A) [UniParc].

Last modified October 24, 2003. Version 2.
Checksum: 7E14D1405B023F96

FASTA46950,755
        10         20         30         40         50         60 
MVLEGNPEVG SPRTSDLQHR GNKGSCVLSS PGEDAQPGEE PIKYGELIVL GCCEEGGEET 

        70         80         90        100        110        120 
EAQRGEVTGP RAHSCYNGCL ASGDKGRRRS RLALSRRSHA NGVKPDVMHH ISTPLVSKAL 

       130        140        150        160        170        180 
SNRGQHSISY TLSRSHSVIV EYTHDSDTDM FQIGRSTENM IDFVVTDTSP GGGAAEGPSA 

       190        200        210        220        230        240 
QSTISRYACR ILCDRRPPYT ARIYAAGFDA SSNIFLGERA AKWRTPDGLM DGLTTNGVLV 

       250        260        270        280        290        300 
MHPAGGFSED SAPGVWREIS VCGNVYTLRD SRSAQQRGKL VENESNVLQD GSLIDLCGAT 

       310        320        330        340        350        360 
LLWRTPAGLL RAPTLKQLEA QRQEANAARP QCPVGLSTLA FPSPARGRTA PDKQQPWVYV 

       370        380        390        400        410        420 
RCGHVHGYHG WGCRRERGPQ ERECPLCRLV GPYVPLWLGQ EAGLCLDPGP PSHAFAPCGH 

       430        440        450        460 
VCSEKTARYW AQTPLPHGTH AFHAACPFCG AWLTGEHGCV RLIFQGPLD 

« Hide

Isoform 2 (Beta) (B) [UniParc].

Checksum: AD1D7E00533CD807
Show »

FASTA44548,237
Isoform 3 [UniParc].

Checksum: EDFD5137B164C41E
Show »

FASTA40643,970
Isoform 4 [UniParc].

Checksum: F0B85D8FF892B233
Show »

FASTA33135,339

References

« Hide 'large scale' references
[1]"Pellino3, a novel member of the Pellino protein family, promotes activation of c-Jun and Elk-1 and may act as a scaffolding protein."
Jensen L.E., Whitehead A.S.
J. Immunol. 171:1500-1506(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH IRAK1; TRAF6; MAP3K7 AND MAP3K14.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Adipose tissue, Thyroid and Uterus.
[3]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Amygdala.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
Tissue: Brain and Lung.
[5]"The IRAK-catalysed activation of the E3 ligase function of Pellino isoforms induces the Lys63-linked polyubiquitination of IRAK1."
Ordureau A., Smith H., Windheim M., Peggie M., Carrick E., Morrice N., Cohen P.
Biochem. J. 409:43-52(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY IRAK1.
[6]"Kinase-active interleukin-1 receptor-associated kinases promote polyubiquitination and degradation of the Pellino family: direct evidence for PELLINO proteins being ubiquitin-protein isopeptide ligases."
Butler M.P., Hanly J.A., Moynagh P.N.
J. Biol. Chem. 282:29729-29737(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF487456 mRNA. Translation: AAO49465.1.
AF487457 mRNA. Translation: AAO49466.1.
AK074201 mRNA. Translation: BAB85015.1.
AK075253 mRNA. Translation: BAC11499.1. Different initiation.
AK094060 mRNA. Translation: BAC04275.1.
AL834395 mRNA. Translation: CAD39057.1.
BC021256 mRNA. Translation: AAH21256.1.
BC025723 mRNA. Translation: AAH25723.1.
RefSeqNP_001091980.1. NM_001098510.1.
NP_001230064.1. NM_001243135.1.
NP_001230065.1. NM_001243136.1.
NP_659502.2. NM_145065.2.
UniGeneHs.656875.

3D structure databases

ProteinModelPortalQ8N2H9.
SMRQ8N2H9. Positions 40-305.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid128900. 11 interactions.
DIPDIP-31770N.
IntActQ8N2H9. 4 interactions.
MINTMINT-2810142.
STRING9606.ENSP00000322532.

PTM databases

PhosphoSiteQ8N2H9.

Polymorphism databases

DMDM37999712.

Proteomic databases

PaxDbQ8N2H9.
PRIDEQ8N2H9.

Protocols and materials databases

DNASU246330.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000320740; ENSP00000322532; ENSG00000174516. [Q8N2H9-1]
ENST00000349459; ENSP00000309848; ENSG00000174516. [Q8N2H9-2]
ENST00000524466; ENSP00000434677; ENSG00000174516. [Q8N2H9-4]
GeneID246330.
KEGGhsa:246330.
UCSCuc001oib.2. human. [Q8N2H9-4]
uc001oic.4. human. [Q8N2H9-1]
uc001oid.4. human. [Q8N2H9-2]
uc021qlx.1. human. [Q8N2H9-3]

Organism-specific databases

CTD246330.
GeneCardsGC11P066234.
HGNCHGNC:30010. PELI3.
HPACAB033750.
MIM609827. gene.
neXtProtNX_Q8N2H9.
PharmGKBPA142671185.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG258040.
HOGENOMHOG000234110.
HOVERGENHBG053559.
InParanoidQ8N2H9.
KOK11964.
OMATSDLQHP.
OrthoDBEOG751NFB.
PhylomeDBQ8N2H9.
TreeFamTF314338.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.
UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ8N2H9.
BgeeQ8N2H9.
CleanExHS_PELI3.
GenevestigatorQ8N2H9.

Family and domain databases

InterProIPR006800. Pellino_fam.
[Graphical view]
PANTHERPTHR12098. PTHR12098. 1 hit.
PfamPF04710. Pellino. 1 hit.
[Graphical view]
PIRSFPIRSF038886. Pellino. 1 hit.
ProtoNetSearch...

Other

ChiTaRSPELI3. human.
GenomeRNAi246330.
NextBio91928.
PROQ8N2H9.
SOURCESearch...

Entry information

Entry namePELI3_HUMAN
AccessionPrimary (citable) accession number: Q8N2H9
Secondary accession number(s): Q8N3E1 expand/collapse secondary AC list , Q8N9Q6, Q8TAW7, Q8TED5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: October 24, 2003
Last modified: April 16, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM