Q8N1V2 (WDR16_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
WD repeat-containing protein 16

Alternative name(s):
WD40-repeat protein up-regulated in HCC

Gene names

Name:	WDR16
Synonyms:	WDRPUH

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	620 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play an essential role in the growth or survival of hepatocellular carcinoma (HCC). Ref.1
Subcellular location	Cytoplasm Ref.1.
Tissue specificity	Highly expressed in testis. Up-regulated in hepatocellular carcinoma (HCC). Ref.1
Miscellaneous	May be a good candidate as a diagnostic marker for HCC as well as a potential molecular target for development of novel therapeutic drugs.
Sequence similarities	Contains 11 WD repeats.
Sequence caution	The sequence BAB85083.1 differs from that shown. Reason: Frameshift at position 183.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat WD repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from direct assay. Source: HPA intracellular membrane-bounded organelle Inferred from direct assay. Source: HPA
Molecular function	protein binding Inferred from physical interaction Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N1V2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N1V2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: MDNKISPEAQVAELELDAVIGFN → MEAVVLPWQIFGVRELPVERGVTMKGPRLFRAP

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8N1V2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     24-91: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 620	620	WD repeat-containing protein 16		PRO_0000233153
Regions
	Repeat	62 – 106	45	WD 1
	Repeat	109 – 150	42	WD 2
	Repeat	156 – 195	40	WD 3
	Repeat	288 – 327	40	WD 4
	Repeat	330 – 369	40	WD 5
	Repeat	372 – 411	40	WD 6
	Repeat	415 – 454	40	WD 7
	Repeat	459 – 498	40	WD 8
	Repeat	500 – 539	40	WD 9
	Repeat	543 – 582	40	WD 10
	Repeat	585 – 620	36	WD 11
Natural variations
	Alternative sequence	1 – 23	23	MDNKI…VIGFN → MEAVVLPWQIFGVRELPVER GVTMKGPRLFRAP in isoform 2.		VSP_018067
	Alternative sequence	24 – 91	68	Missing in isoform 3.		VSP_018068
	Natural variant	336	1	E → K. Ref.2 Ref.4 Corresponds to variant rs6503235 [ dbSNP | Ensembl ].		VAR_026057
Experimental info
	Sequence conflict	91	1	A → V in AAH25392. Ref.4
	Sequence conflict	199	1	C → S in AAH25392. Ref.4
	Sequence conflict	243	1	G → E in BAB85083. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2007. Version 3. Checksum: DC2F5DC3994CD7DD	FASTA	620	68,298
	10 20 30 40 50 60 MDNKISPEAQ VAELELDAVI GFNGHVPTGL KCHPDQEHMI YPLGCTVLIQ AINTKEQNFL 70 80 90 100 110 120 QGHGNNVSCL AISRSGEYIA SGQVTFMGFK ADIILWDYKN RELLARLSLH KGKIEALAFS 130 140 150 160 170 180 PNDLYLVSLG GPDDGSVVVW SIAKRDAICG SPAAGLNVGN ATNVIFSRCR DEMFMTAGNG 190 200 210 220 230 240 TIRVWELDLP NRKIWPTECQ TGQLKRIVMS IGVDDDDSFF YLGTTTGDIL KMNPRTKLLT 250 260 270 280 290 300 DVGPAKDKFS LGVSAIRCLK MGGLLVGSGA GLLVFCKSPG YKPIKKIQLQ GGITSITLRG 310 320 330 340 350 360 EGHQFLVGTE ESHIYRVSFT DFKETLIATC HFDAVEDIVF PFGTAELFAT CAKKDIRVWH 370 380 390 400 410 420 TSSNRELLRI TVPNMTCHGI DFMRDGKSII SAWNDGKIRA FAPETGRLMY VINNAHRIGV 430 440 450 460 470 480 TAIATTSDCK RVISGGGEGE VRVWQIGCQT QKLEEALKEH KSSVSCIRVK RNNEECVTAS 490 500 510 520 530 540 TDGTCIIWDL VRLRRNQMIL ANTLFQCVCY HPEEFQIITS GTDRKIAYWE VFDGTVIREL 550 560 570 580 590 600 EGSLSGSING MDITQEGVHF VTGGNDHLVK VWDYNEGEVT HVGVGHSGNI TRIRISPGNQ 610 620 YIVSVSADGA ILRWKYPYTS
	Isoform 2 [UniParc]. Checksum: 9FA6195B75B4D0C4	FASTA	630	69,575
	10 20 30 40 50 60 MEAVVLPWQI FGVRELPVER GVTMKGPRLF RAPGHVPTGL KCHPDQEHMI YPLGCTVLIQ 70 80 90 100 110 120 AINTKEQNFL QGHGNNVSCL AISRSGEYIA SGQVTFMGFK ADIILWDYKN RELLARLSLH 130 140 150 160 170 180 KGKIEALAFS PNDLYLVSLG GPDDGSVVVW SIAKRDAICG SPAAGLNVGN ATNVIFSRCR 190 200 210 220 230 240 DEMFMTAGNG TIRVWELDLP NRKIWPTECQ TGQLKRIVMS IGVDDDDSFF YLGTTTGDIL 250 260 270 280 290 300 KMNPRTKLLT DVGPAKDKFS LGVSAIRCLK MGGLLVGSGA GLLVFCKSPG YKPIKKIQLQ 310 320 330 340 350 360 GGITSITLRG EGHQFLVGTE ESHIYRVSFT DFKETLIATC HFDAVEDIVF PFGTAELFAT 370 380 390 400 410 420 CAKKDIRVWH TSSNRELLRI TVPNMTCHGI DFMRDGKSII SAWNDGKIRA FAPETGRLMY 430 440 450 460 470 480 VINNAHRIGV TAIATTSDCK RVISGGGEGE VRVWQIGCQT QKLEEALKEH KSSVSCIRVK 490 500 510 520 530 540 RNNEECVTAS TDGTCIIWDL VRLRRNQMIL ANTLFQCVCY HPEEFQIITS GTDRKIAYWE 550 560 570 580 590 600 VFDGTVIREL EGSLSGSING MDITQEGVHF VTGGNDHLVK VWDYNEGEVT HVGVGHSGNI 610 620 630 TRIRISPGNQ YIVSVSADGA ILRWKYPYTS
	Isoform 3 [UniParc]. Checksum: F67217BF3FC71AC8	FASTA	552	60,957
	10 20 30 40 50 60 MDNKISPEAQ VAELELDAVI GFNDIILWDY KNRELLARLS LHKGKIEALA FSPNDLYLVS 70 80 90 100 110 120 LGGPDDGSVV VWSIAKRDAI CGSPAAGLNV GNATNVIFSR CRDEMFMTAG NGTIRVWELD 130 140 150 160 170 180 LPNRKIWPTE CQTGQLKRIV MSIGVDDDDS FFYLGTTTGD ILKMNPRTKL LTDVGPAKDK 190 200 210 220 230 240 FSLGVSAIRC LKMGGLLVGS GAGLLVFCKS PGYKPIKKIQ LQGGITSITL RGEGHQFLVG 250 260 270 280 290 300 TEESHIYRVS FTDFKETLIA TCHFDAVEDI VFPFGTAELF ATCAKKDIRV WHTSSNRELL 310 320 330 340 350 360 RITVPNMTCH GIDFMRDGKS IISAWNDGKI RAFAPETGRL MYVINNAHRI GVTAIATTSD 370 380 390 400 410 420 CKRVISGGGE GEVRVWQIGC QTQKLEEALK EHKSSVSCIR VKRNNEECVT ASTDGTCIIW 430 440 450 460 470 480 DLVRLRRNQM ILANTLFQCV CYHPEEFQII TSGTDRKIAY WEVFDGTVIR ELEGSLSGSI 490 500 510 520 530 540 NGMDITQEGV HFVTGGNDHL VKVWDYNEGE VTHVGVGHSG NITRIRISPG NQYIVSVSAD 550 GAILRWKYPY TS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"WDRPUH, a novel WD-repeat-containing protein, is highly expressed in human hepatocellular carcinoma and involved in cell proliferation." Silva F.P., Hamamoto R., Nakamura Y., Furukawa Y. Neoplasia 7:348-355(2005) [PubMed: 15967112] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH HSP70; CCT CHAPERONIN COMPLEX AND BRCA2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3), VARIANT LYS-336. Tissue: Caudate nucleus, Lung and Testis.
[3]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed: 16625196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT LYS-336. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB065281 mRNA. Translation: BAB83743.1. AK074435 mRNA. Translation: BAB85083.1. Frameshift. AK094847 mRNA. Translation: BAC04435.1. AK315679 mRNA. Translation: BAG38044.1. AC087501 Genomic DNA. No translation available. AC118755 Genomic DNA. No translation available. BC025392 mRNA. Translation: AAH25392.1.
IPI	IPI00170961. IPI00184719. IPI00745344.
RefSeq	NP_001074025.1. NM_001080556.1. NP_659491.4. NM_145054.4.
UniGene	Hs.232270.
3D structure databases
ProteinModelPortal	Q8N1V2.
SMR	Q8N1V2. Positions 28-146, 335-618.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8N1V2.
PTM databases
PhosphoSite	Q8N1V2.
Polymorphism databases
DMDM	146291099.
Proteomic databases
PRIDE	Q8N1V2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000352665; ENSP00000339449; ENSG00000166596.
GeneID	146845.
KEGG	hsa:146845.
UCSC	uc002gly.1. human. uc002glz.1. human. uc010coc.1. human.
Organism-specific databases
CTD	146845.
GeneCards	GC17P009422.
H-InvDB	HIX0013530.
HGNC	HGNC:16053. WDR16.
HPA	HPA023247. HPA023562.
MIM	609804. gene.
neXtProt	NX_Q8N1V2.
PharmGKB	PA38084.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG07909.
GeneTree	ENSGT00530000063072.
HOVERGEN	HBG055224.
OMA	MADDDSF.
OrthoDB	EOG4SBDXM.
PhylomeDB	Q8N1V2.
Gene expression databases
ArrayExpress	Q8N1V2.
Bgee	Q8N1V2.
CleanEx	HS_WDR16.
Genevestigator	Q8N1V2.
GermOnline	ENSG00000166596. Homo sapiens.
Family and domain databases
InterPro	IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 2 hits.
Pfam	PF00400. WD40. 7 hits. [Graphical view]
SMART	SM00320. WD40. 11 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 2 hits.
PROSITE	PS00678. WD_REPEATS_1. 2 hits. PS50082. WD_REPEATS_2. 5 hits. PS50294. WD_REPEATS_REGION. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	85465.
SOURCE	Search...

Entry information

Entry name

WDR16_HUMAN

Accession

Primary (citable) accession number: Q8N1V2
Secondary accession number(s): B2RDU7 , Q5DX23, Q8TC73, Q8TCI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	May 1, 2007
Last modified:	January 25, 2012
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.