Q8N1N5 (CRPAK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine-rich PAK1 inhibitor
Short name=CRIPak

Gene names

Name:

CRIPAK

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Negative regulator of PAK1. It has been suggested that the lost of CRIPAK in breast tumors might contribute to hormonal independence. Ref.2
Subunit structure	Interacts with PAK1. Ref.2
Subcellular location	Cytoplasm. Membrane. Nucleus. Note: Translocated to the nuclear compartment upon hormonal treatment by estrogen. Ref.2
Tissue specificity	Widely expressed with a highest expression observed in trachea, prostate and adrenal glands. Expressed in many cancer cell lines including breast cancer cells. Ref.2
Induction	Up-regulated by estrogen. Ref.2

Ontologies

Keywords
Cellular component	Cytoplasm Membrane Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ER-nucleus signaling pathway Inferred from direct assay. Source: HGNC negative regulation of protein kinase activity Inferred from direct assay Ref.2. Source: HGNC regulation of cytoskeleton organization Inferred from direct assay Ref.2. Source: HGNC response to estrogen stimulus Inferred from direct assay Ref.2. Source: HGNC
Cellular component	endoplasmic reticulum Inferred from direct assay Ref.2. Source: HGNC nucleus Inferred from direct assay Ref.2. Source: HGNC plasma membrane Inferred from direct assay Ref.2. Source: HGNC
Molecular function	protein binding Inferred from physical interaction Ref.2. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PAK1	Q13153	6	EBI-1205846,EBI-1307

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Cysteine-rich PAK1 inhibitor		PRO_0000318594
Regions
	Repeat	34 – 50	17	1
	Repeat	106 – 122	17	2
	Repeat	137 – 153	17	3
	Repeat	168 – 184	17	4
	Repeat	199 – 215	17	5
	Repeat	230 – 246	17	6
	Repeat	250 – 266	17	7
	Repeat	270 – 286	17	8
	Repeat	321 – 337	17	9
	Repeat	374 – 390	17	10
	Region	367 – 446	80	Interaction with PAK1
Natural variations
	Natural variant	89	1	C → Y. Corresponds to variant rs13097 [ dbSNP | Ensembl ].		VAR_038794
	Natural variant	95	1	H → P. Corresponds to variant rs9328733 [ dbSNP | Ensembl ].		VAR_038795
	Natural variant	95	1	H → R. Corresponds to variant rs9328733 [ dbSNP | Ensembl ].		VAR_061628
	Natural variant	120	1	V → M. Corresponds to variant rs13097 [ dbSNP | Ensembl ].		VAR_050908
Experimental info
	Sequence conflict	358	1	H → Y in BAC03741. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8N1N5 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 92EDA1B7B8037FDF	FASTA	446	48,076
	10 20 30 40 50 60 MHEPSLCANV ECPPAHTCPC GVPACSCAHV ECPPAHTCRC GVPACSHMPM WSARLLTRAH 70 80 90 100 110 120 VECPPAHTRV HVECPPAHVP MWSAHLLTCA DVECHLLTHV PMWSARLLTC PCGVPACSHV 130 140 150 160 170 180 PMRSARLLTR AHAECPPAHT CPCGVPACSH VPMRSARLLT RADVECPPAH TCPCGVPACS 190 200 210 220 230 240 HVPTWSARLI TRAHVECSPA HTCRCGVPAC SHVPMWSVRL LTRADAECPP AHTCRCGVPA 250 260 270 280 290 300 CSHVPMWSAR LLTCRCGVPA CSHVPMWSAR LLTCRCGVPA CSHVPMWSAR LLTRAHVECP 310 320 330 340 350 360 PAHTCRRGVP ACSRAHMECP PAHTCHCGVP ACSHTCRCGV PACSHVPMWS ARLLTRAHVE 370 380 390 400 410 420 CPPAHTRAHV ECPPAHTCPC GVPACSHTCP CGVPACSHKA LAWWFCRFPV LPAESDAVTV 430 440 HSTHGGFLIR FYVKDPFYIS LHLEIT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Oesophagus.
[2]	"CRIPak, a novel endogenous Pak1 inhibitor." Talukder A.H., Meng Q., Kumar R. Oncogene 25:1311-1319(2006) [PubMed: 16278681] [Abstract] Cited for: FUNCTION, INTERACTION WITH PAK1, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION BY ESTROGEN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK091762 mRNA. Translation: BAC03741.1. AK096209 mRNA. Translation: BAC04725.1.
IPI	IPI00184021.
RefSeq	NP_787114.2. NM_175918.3.
UniGene	Hs.26410.
3D structure databases
ProteinModelPortal	Q8N1N5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8N1N5. 14 interactions.
STRING	Q8N1N5.
Polymorphism databases
DMDM	74728555.
Proteomic databases
PRIDE	Q8N1N5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000324803; ENSP00000323978; ENSG00000179979.
GeneID	285464.
KEGG	hsa:285464.
UCSC	uc003gdf.2. human.
Organism-specific databases
CTD	285464.
GeneCards	GC04P001378.
H-InvDB	HIX0004022.
HGNC	HGNC:26619. CRIPAK.
MIM	610203. gene.
neXtProt	NX_Q8N1N5.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00570000080097.
HOVERGEN	HBG064261.
InParanoid	Q8N1N5.
OMA	GVPACSH.
OrthoDB	EOG4933HX.
PhylomeDB	Q8N1N5.
Gene expression databases
ArrayExpress	Q8N1N5.
Bgee	Q8N1N5.
CleanEx	HS_CRIPAK.
Genevestigator	Q8N1N5.
Family and domain databases
InterPro	IPR003616. Post-SET_dom. [Graphical view]
SMART	SM00508. PostSET. 10 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	95535.
SOURCE	Search...

Entry information

Entry name

CRPAK_HUMAN

Accession

Primary (citable) accession number: Q8N1N5
Secondary accession number(s): Q8NB03

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	October 1, 2002
Last modified:	December 14, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.