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Protein

Keratin, type II cytoskeletal 78

Gene

KRT78

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin, I (acidic) and II (neutral to basic) (40-55 and 56-70 kDa, respectively).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei362Stutter1

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-6805567. Keratinization.
R-HSA-6809371. Formation of the cornified envelope.

Names & Taxonomyi

Protein namesi
Recommended name:
Keratin, type II cytoskeletal 78
Alternative name(s):
Cytokeratin-78
Short name:
CK-78
Keratin-5b
Keratin-78
Short name:
K78
Type-II keratin Kb40
Gene namesi
Name:KRT78
Synonyms:K5B, KB40
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:28926. KRT78.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: UniProtKB
  • keratin filament Source: InterPro

Keywords - Cellular componenti

Intermediate filament, Keratin

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000170423.
PharmGKBiPA147357807.

Polymorphism and mutation databases

BioMutaiKRT78.
DMDMi166218809.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003148911 – 520Keratin, type II cytoskeletal 78Add BLAST520

Proteomic databases

EPDiQ8N1N4.
PaxDbiQ8N1N4.
PeptideAtlasiQ8N1N4.
PRIDEiQ8N1N4.

PTM databases

iPTMnetiQ8N1N4.
PhosphoSitePlusiQ8N1N4.
SwissPalmiQ8N1N4.

Expressioni

Tissue specificityi

In non-keratinising esophageal and vaginal epithelium, strongly expressed in the basal and parabasal/lower suprabasal cell layers with considerably decreased expression in the mid/upper suprabasal layers (at protein level) (PubMed:26340985). A similar gradient from basal to lower suprabasal layers is seen in the partially keratinised dorsal tongue epithelium, in the scalp and in the plantar epidermis (at protein level) (PubMed:26340985). Extension of expression into the suprabasal compartments is distinctly more pronounced in non-keratinising epithelia than in keratinising epithelia and epidermis (at protein level) (PubMed:26340985). In scalp sections, present in the interfollicular epidermis and infundibulum including the entire outer root sheath of the hair follicles and also in the sebocytes (at protein level) (PubMed:26340985). In sweat glands, expressed in peripheral and luminal cells of the lower duct and in peripheral cells of the middle/upper duct with no expression observed in luminal cells (at protein level) (PubMed:26340985). In embryos at the 14th week of pregnancy, detected in basal and parabasal layers but is absent from the uppermost epidermal layer (at protein level) (PubMed:26340985). Expressed in tongue epithelium (PubMed:15737194).2 Publications

Gene expression databases

BgeeiENSG00000170423.
CleanExiHS_KRT78.
ExpressionAtlasiQ8N1N4. baseline and differential.
GenevisibleiQ8N1N4. HS.

Organism-specific databases

HPAiHPA039724.

Interactioni

Subunit structurei

Heterotetramer of two type I and two type II keratins.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi128199. 11 interactors.
IntActiQ8N1N4. 19 interactors.
STRINGi9606.ENSP00000306261.

Structurei

3D structure databases

ProteinModelPortaliQ8N1N4.
SMRiQ8N1N4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 110HeadAdd BLAST110
Regioni111 – 421RodAdd BLAST311
Regioni111 – 146Coil 1AAdd BLAST36
Regioni147 – 165Linker 1Add BLAST19
Regioni166 – 258Coil 1BAdd BLAST93
Regioni259 – 281Linker 12Add BLAST23
Regioni282 – 421Coil 2Add BLAST140
Regioni422 – 520TailAdd BLAST99

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi7 – 82Gly-richAdd BLAST76
Compositional biasi430 – 507Ser-richAdd BLAST78

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IHY4. Eukaryota.
ENOG4111DA8. LUCA.
GeneTreeiENSGT00830000128228.
HOGENOMiHOG000230976.
HOVERGENiHBG013015.
InParanoidiQ8N1N4.
KOiK07605.
OMAiLNSFGGC.
OrthoDBiEOG091G0BSH.
PhylomeDBiQ8N1N4.
TreeFamiTF317854.

Family and domain databases

InterProiView protein in InterPro
IPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR032444. Keratin_2_head.
IPR003054. Keratin_II.
PANTHERiPTHR23239. PTHR23239. 1 hit.
PfamiView protein in Pfam
PF00038. Filament. 1 hit.
PF16208. Keratin_2_head. 1 hit.
PRINTSiPR01276. TYPE2KERATIN.
SMARTiView protein in SMART
SM01391. Filament. 1 hit.
PROSITEiView protein in PROSITE
PS00226. IF. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N1N4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLSPCRAQR GFSARSACSA RSRGRSRGGF SSRGGFSSRS LNSFGGCLEG
60 70 80 90 100
SRGSTWGSGG RLGVRFGEWS GGPGLSLCPP GGIQEVTINQ NLLTPLKIEI
110 120 130 140 150
DPQFQVVRTQ ETQEIRTLNN QFASFIDKVR FLEQQNKVLE TKWHLLQQQG
160 170 180 190 200
LSGSQQGLEP VFEACLDQLR KQLEQLQGER GALDAELKAC RDQEEEYKSK
210 220 230 240 250
YEEEAHRRAT LENDFVVLKK DVDGVFLSKM ELEGKLEALR EYLYFLKHLN
260 270 280 290 300
EEELGQLQTQ ASDTSVVLSM DNNRYLDFSS IITEVRARYE EIARSSKAEA
310 320 330 340 350
EALYQTKYQE LQVSAQLHGD RMQETKVQIS QLHQEIQRLQ SQTENLKKQN
360 370 380 390 400
ASLQAAITDA EQRGELALKD AQAKVDELEA ALRMAKQNLA RLLCEYQELT
410 420 430 440 450
STKLSLDVEI ATYRRLLEGE ECRMSGECTS QVTISSVGGS AVMSGGVGGG
460 470 480 490 500
LGSTCGLGSG KGSPGSCCTS IVTGGSNIIL GSGKDPVLDS CSVSGSSAGS
510 520
SCHTILKKTV ESSLKTSITY
Length:520
Mass (Da):56,866
Last modified:January 15, 2008 - v2
Checksum:i4502110AAA408E86
GO
Isoform 2 (identifier: Q8N1N4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-110: Missing.
     111-127: ETQEIRTLNNQFASFID → MEGHEASPAQVGQGDRG

Note: No experimental confirmation available.
Show »
Length:410
Mass (Da):45,017
Checksum:iAF958D13D971A4A8
GO

Sequence cautioni

The sequence DAA00377 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti250N → S in CAI46001 (PubMed:17974005).Curated1
Sequence conflicti259T → A in BAC04782 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03810925R → H. Corresponds to variant dbSNP:rs11170289Ensembl.1
Natural variantiVAR_03811092L → P1 PublicationCorresponds to variant dbSNP:rs2013335Ensembl.1
Natural variantiVAR_038111224G → A. Corresponds to variant dbSNP:rs2682343Ensembl.1
Natural variantiVAR_038112238A → T. Corresponds to variant dbSNP:rs10876360Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0304191 – 110Missing in isoform 2. 1 PublicationAdd BLAST110
Alternative sequenceiVSP_030420111 – 127ETQEI…ASFID → MEGHEASPAQVGQGDRG in isoform 2. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK096419 mRNA. Translation: BAC04782.1.
AK290901 mRNA. Translation: BAF83590.1.
BX647095 mRNA. Translation: CAI46001.1.
AC107016 Genomic DNA. No translation available.
BK000646 Genomic DNA. Translation: DAA00377.1. Sequence problems.
CH471054 Genomic DNA. Translation: EAW96650.1.
CCDSiCCDS73473.1. [Q8N1N4-2]
CCDS8840.1. [Q8N1N4-1]
RefSeqiNP_001287743.1. NM_001300814.1. [Q8N1N4-2]
NP_775487.2. NM_173352.3. [Q8N1N4-1]
UniGeneiHs.665267.

Genome annotation databases

EnsembliENST00000304620; ENSP00000306261; ENSG00000170423. [Q8N1N4-1]
ENST00000359499; ENSP00000352479; ENSG00000170423. [Q8N1N4-2]
GeneIDi196374.
KEGGihsa:196374.
UCSCiuc001sbc.1. human. [Q8N1N4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiK2C78_HUMAN
AccessioniPrimary (citable) accession number: Q8N1N4
Secondary accession number(s): A8K4D6, Q5HYM7, Q7RTT2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: August 30, 2017
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families