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Protein

Dedicator of cytokinesis protein 4

Gene

DOCK4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in regulation of adherens junction between cells. Plays a role in cell migration. Functions as a guanine nucleotide exchange factor (GEF), which activates Rap1 small GTPase by exchanging bound GDP for free GTP.2 Publications

GO - Molecular functioni

  • GTPase activator activity Source: HGNC
  • guanyl-nucleotide exchange factor activity Source: UniProtKB-KW
  • PDZ domain binding Source: HGNC
  • Rac GTPase binding Source: HGNC
  • receptor tyrosine kinase binding Source: UniProtKB

GO - Biological processi

  • cell chemotaxis Source: UniProtKB
  • negative regulation of vascular smooth muscle contraction Source: BHF-UCL
  • positive regulation of vascular associated smooth muscle cell migration Source: BHF-UCL
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

BioCyciZFISH:ENSG00000128512-MONOMER.
ReactomeiR-HSA-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Dedicator of cytokinesis protein 4
Gene namesi
Name:DOCK4
Synonyms:KIAA0716
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:19192. DOCK4.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
  • stereocilium Source: HGNC
  • stereocilium bundle Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

DisGeNETi9732.
OpenTargetsiENSG00000128512.
PharmGKBiPA134939318.

Polymorphism and mutation databases

BioMutaiDOCK4.
DMDMi296439369.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001899901 – 1966Dedicator of cytokinesis protein 4Add BLAST1966

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei167PhosphotyrosineBy similarity1
Modified residuei193PhosphothreonineBy similarity1
Modified residuei1599PhosphoserineBy similarity1
Modified residuei1607PhosphoserineBy similarity1
Modified residuei1614PhosphoserineCombined sources1
Modified residuei1618PhosphoserineCombined sources1
Modified residuei1620PhosphoserineCombined sources1
Modified residuei1631PhosphoserineBy similarity1
Modified residuei1769PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8N1I0.
MaxQBiQ8N1I0.
PaxDbiQ8N1I0.
PeptideAtlasiQ8N1I0.
PRIDEiQ8N1I0.

PTM databases

iPTMnetiQ8N1I0.
PhosphoSitePlusiQ8N1I0.

Expressioni

Tissue specificityi

Widely expressed at low level. Highly expressed in skeletal muscle, prostate and ovary.1 Publication

Gene expression databases

BgeeiENSG00000128512.
CleanExiHS_DOCK4.
ExpressionAtlasiQ8N1I0. baseline and differential.
GenevisibleiQ8N1I0. HS.

Interactioni

Subunit structurei

Interacts with the SH3 domain of CRK. Interacts with nucleotide-free Rap1. Interacts with FASLG. Interacts with ELMO2 and EPHA2; mediates activation of RAC1 by EPHA2.3 Publications

GO - Molecular functioni

  • PDZ domain binding Source: HGNC
  • Rac GTPase binding Source: HGNC
  • receptor tyrosine kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi115081. 17 interactors.
IntActiQ8N1I0. 2 interactors.
MINTiMINT-1632264.
STRINGi9606.ENSP00000404179.

Structurei

3D structure databases

ProteinModelPortaliQ8N1I0.
SMRiQ8N1I0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 67SH3PROSITE-ProRule annotationAdd BLAST62
Domaini401 – 574DHR-1PROSITE-ProRule annotationAdd BLAST174
Domaini1190 – 1596DHR-2PROSITE-ProRule annotationAdd BLAST407

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1788 – 1794SH3-bindingSequence analysis7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1642 – 1863Ser-richAdd BLAST222
Compositional biasi1875 – 1960Pro-richAdd BLAST86

Domaini

The DHR-2 domain probably mediates the GEF activity.By similarity

Sequence similaritiesi

Belongs to the DOCK family.PROSITE-ProRule annotation
Contains 1 DHR-1 domain.PROSITE-ProRule annotation
Contains 1 DHR-2 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain, SH3-binding

Phylogenomic databases

eggNOGiKOG1998. Eukaryota.
ENOG410XQH7. LUCA.
GeneTreeiENSGT00610000085806.
HOGENOMiHOG000006631.
HOVERGENiHBG051389.
InParanoidiQ8N1I0.
KOiK17697.
PhylomeDBiQ8N1I0.
TreeFamiTF300423.

Family and domain databases

InterProiIPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR010703. DOCK_C.
IPR032376. DOCK_N.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23317. PTHR23317. 1 hit.
PfamiPF06920. DHR-2. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF16172. DOCK_N. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N1I0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWIPTEHEKY GVVIASFRGT VPYGLSLEIG DTVQILEKCD GWYRGFALKN
60 70 80 90 100
PNIKGIFPSS YVHLKNACVK NKGQFEMVIP TEDSVITEMT STLRDWGTMW
110 120 130 140 150
KQLYVRNEGD LFHRLWHIMN EILDLRRQVL VGHLTHDRMK DVKRHITARL
160 170 180 190 200
DWGNEQLGLD LVPRKEYAMV DPEDISITEL YRLMEHRHRK KDTPVQASSH
210 220 230 240 250
HLFVQMKSLM CSNLGEELEV IFSLFDSKEN RPISERFFLR LNRNGLPKAP
260 270 280 290 300
DKPERHCSLF VDLGSSELRK DIYITVHIIR IGRMGAGEKK NACSVQYRRP
310 320 330 340 350
FGCAVLSIAD LLTGETKDDL ILKVYMCNTE SEWYQIHENI IKKLNARYNL
360 370 380 390 400
TGSNAGLAVS LQLLHGDIEQ IRREYSSVFS HGVSITRKLG FSNIIMPGEM
410 420 430 440 450
RNDLYITIER GEFEKGGKSV ARNVEVTMFI VDSSGQTLKD FISFGSGEPP
460 470 480 490 500
ASEYHSFVLY HNNSPRWSEL LKLPIPVDKF RGAHIRFEFR HCSTKEKGEK
510 520 530 540 550
KLFGFSFVPL MQEDGRTLPD GTHELIVHKC EENTNLQDTT RYLKLPFSKG
560 570 580 590 600
IFLGNNNQAM KATKESFCIT SFLCSTKLTQ NGDMLDLLKW RTHPDKITGC
610 620 630 640 650
LSKLKEIDGS EIVKFLQDTL DTLFGILDEN SQKYGSKVFD SLVHIINLLQ
660 670 680 690 700
DSKFHHFKPV MDTYIESHFA GALAYRDLIK VLKWYVDRIT EAERQEHIQE
710 720 730 740 750
VLKAQEYIFK YIVQSRRLFS LATGGQNEEE FRCCIQELLM SVRFFLSQES
760 770 780 790 800
KGSGALSQSQ AVFLSSFPAV YSELLKLFDV REVANLVQDT LGSLPTILHV
810 820 830 840 850
DDSLQAIKLQ CIGKTVESQL YTNPDSRYIL LPVVLHHLHI HLQEQKDLIM
860 870 880 890 900
CARILSNVFC LIKKNSSEKS VLEEIDVIVA SLLDILLRTI LEITSRPQPS
910 920 930 940 950
SSAMRFQFQD VTGEFVACLL SLLRQMTDRH YQQLLDSFNT KEELRDFLLQ
960 970 980 990 1000
IFTVFRILIR PEMFPKDWTV MRLVANNVII TTVLYLSDAL RKNFLNENFD
1010 1020 1030 1040 1050
YKIWDSYFYL AVIFINQLCL QLEMFTPSKK KKVLEKYGDM RVTMGCEIFS
1060 1070 1080 1090 1100
MWQNLGEHKL HFIPALIGPF LEVTLIPQPD LRNVMIPIFH DMMDWEQRRS
1110 1120 1130 1140 1150
GNFKQVEAKL IDKLDSLMSE GKGDETYREL FNSILLKKIE RETWRESGVS
1160 1170 1180 1190 1200
LIATVTRLME RLLDYRDCMK MGEVDGKKIG CTVSLLNFYK TELNKEEMYI
1210 1220 1230 1240 1250
RYIHKLYDLH LKAQNFTEAA YTLLLYDELL EWSDRPLREF LTYPMQTEWQ
1260 1270 1280 1290 1300
RKEHLHLTII QNFDRGKCWE NGIILCRKIA EQYESYYDYR NLSKMRMMEA
1310 1320 1330 1340 1350
SLYDKIMDQQ RLEPEFFRVG FYGKKFPFFL RNKEFVCRGH DYERLEAFQQ
1360 1370 1380 1390 1400
RMLNEFPHAI AMQHANQPDE TIFQAEAQYL QIYAVTPIPE SQEVLQREGV
1410 1420 1430 1440 1450
PDNIKSFYKV NHIWKFRYDR PFHKGTKDKE NEFKSLWVER TSLYLVQSLP
1460 1470 1480 1490 1500
GISRWFEVEK REVVEMSPLE NAIEVLENKN QQLKTLISQC QTRQMQNINP
1510 1520 1530 1540 1550
LTMCLNGVID AAVNGGVSRY QEAFFVKEYI LSHPEDGEKI ARLRELMLEQ
1560 1570 1580 1590 1600
AQILEFGLAV HEKFVPQDMR PLHKKLVDQF FVMKSSLGIQ EFSACMQASP
1610 1620 1630 1640 1650
VHFPNGSPRV CRNSAPASVS PDGTRVIPRR SPLSYPAVNR YSSSSLSSQA
1660 1670 1680 1690 1700
SAEVSNITGQ SESSDEVFNM QPSPSTSSLS STHSASPNVT SSAPSSARAS
1710 1720 1730 1740 1750
PLLSDKHKHS RENSCLSPRE RPCSAIYPTP VEPSQRMLFN HIGDGALPRS
1760 1770 1780 1790 1800
DPNLSAPEKA VNPTPSSWSL DSGKEAKNMS DSGKLISPPV PPRPTQTASP
1810 1820 1830 1840 1850
ARHTTSVSPS PAGRSPLKGS VQSFTPSPVE YHSPGLISNS PVLSGSYSSG
1860 1870 1880 1890 1900
ISSLSRCSTS ETSGFENQVN EQSAPLPVPV PVPVPSYGGE EPVRKESKTP
1910 1920 1930 1940 1950
PPYSVYERTL RRPVPLPHSL SIPVTSEPPA LPPKPLAARS SHLENGARRT
1960
DPGPRPRPLP RKVSQL
Length:1,966
Mass (Da):225,206
Last modified:May 18, 2010 - v3
Checksum:i9CFB6299F7730AA9
GO
Isoform 2 (identifier: Q8N1I0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1134-1134: I → IIPLFGPYPS
     1760-1797: Missing.

Note: No experimental confirmation available.
Show »
Length:1,937
Mass (Da):222,218
Checksum:i544451D8A8B23F09
GO
Isoform 3 (identifier: Q8N1I0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1134-1134: I → IIPLFGPYPS

Note: No experimental confirmation available.
Show »
Length:1,975
Mass (Da):226,178
Checksum:i535C971462E903DB
GO
Isoform 4 (identifier: Q8N1I0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1687: Missing.
     1688-1698: NVTSSAPSSAR → MLMILSLLLFP

Note: No experimental confirmation available.
Show »
Length:279
Mass (Da):29,833
Checksum:i52A4E7E9F1738323
GO

Sequence cautioni

The sequence BAC03696 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti945R → K in AAB83946 (PubMed:12853948).Curated1
Sequence conflicti1080D → G in AAO73565 (PubMed:12628187).Curated1
Sequence conflicti1122K → E in BAC03696 (PubMed:14702039).Curated1
Sequence conflicti1379 – 1381YLQ → CIRTYKGWTQFGTAVITDVG RLGTQIITQIN in BAC05221 (PubMed:12853948).Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01582387T → I Found in a CNS cancer cell line. 1 Publication1
Natural variantiVAR_057517535N → D.Corresponds to variant rs12705801dbSNPEnsembl.1
Natural variantiVAR_015824606E → Q.1 Publication1
Natural variantiVAR_057518853R → H.Corresponds to variant rs2074130dbSNPEnsembl.1
Natural variantiVAR_0158251059K → T Found in a CNS cancer cell line. 1 Publication1
Natural variantiVAR_0575191570R → K.Corresponds to variant rs3757650dbSNPEnsembl.1
Natural variantiVAR_0575201580F → L.Corresponds to variant rs3757651dbSNPEnsembl.1
Natural variantiVAR_0158261718P → L Found in prostate and ovarian cancer cell lines; abolishes ability to interact with CRK and to activate Rap1. 1 Publication1
Natural variantiVAR_0158271733P → A.1 PublicationCorresponds to variant rs150569245dbSNPEnsembl.1
Natural variantiVAR_0158281755S → P Found in colorectal cancer cell line. 1 Publication1
Natural variantiVAR_0575211822Q → K.Corresponds to variant rs10281942dbSNPEnsembl.1
Natural variantiVAR_0158291884V → M Found in a prostate cancer cell line. 1 PublicationCorresponds to variant rs369715294dbSNPEnsembl.1
Natural variantiVAR_0158301914V → I.1 PublicationCorresponds to variant rs12705795dbSNPEnsembl.1
Natural variantiVAR_0158311917P → L.1 PublicationCorresponds to variant rs199706346dbSNPEnsembl.1
Natural variantiVAR_0158321926S → L.1 PublicationCorresponds to variant rs34597439dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0077011 – 1687Missing in isoform 4. 1 PublicationAdd BLAST1687
Alternative sequenceiVSP_0077031134I → IIPLFGPYPS in isoform 2 and isoform 3. 2 Publications1
Alternative sequenceiVSP_0077051688 – 1698NVTSSAPSSAR → MLMILSLLLFP in isoform 4. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_0077061760 – 1797Missing in isoform 2. 1 PublicationAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY233380 mRNA. Translation: AAO73565.1.
AK091557 mRNA. Translation: BAC03696.1. Different initiation.
AK098050 mRNA. Translation: BAC05221.1.
AC003077 Genomic DNA. Translation: AAB83946.1.
AC003080 Genomic DNA. No translation available.
AC004111 Genomic DNA. No translation available.
AC005047 Genomic DNA. No translation available.
AB018259 mRNA. Translation: BAA34436.3.
CCDSiCCDS47688.1. [Q8N1I0-1]
PIRiT01438.
RefSeqiNP_055520.3. NM_014705.3. [Q8N1I0-1]
XP_006716251.1. XM_006716188.2. [Q8N1I0-3]
XP_006716252.1. XM_006716189.2. [Q8N1I0-2]
UniGeneiHs.654652.

Genome annotation databases

EnsembliENST00000428084; ENSP00000410746; ENSG00000128512. [Q8N1I0-3]
ENST00000437633; ENSP00000404179; ENSG00000128512. [Q8N1I0-1]
GeneIDi9732.
KEGGihsa:9732.
UCSCiuc003vfx.4. human. [Q8N1I0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY233380 mRNA. Translation: AAO73565.1.
AK091557 mRNA. Translation: BAC03696.1. Different initiation.
AK098050 mRNA. Translation: BAC05221.1.
AC003077 Genomic DNA. Translation: AAB83946.1.
AC003080 Genomic DNA. No translation available.
AC004111 Genomic DNA. No translation available.
AC005047 Genomic DNA. No translation available.
AB018259 mRNA. Translation: BAA34436.3.
CCDSiCCDS47688.1. [Q8N1I0-1]
PIRiT01438.
RefSeqiNP_055520.3. NM_014705.3. [Q8N1I0-1]
XP_006716251.1. XM_006716188.2. [Q8N1I0-3]
XP_006716252.1. XM_006716189.2. [Q8N1I0-2]
UniGeneiHs.654652.

3D structure databases

ProteinModelPortaliQ8N1I0.
SMRiQ8N1I0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115081. 17 interactors.
IntActiQ8N1I0. 2 interactors.
MINTiMINT-1632264.
STRINGi9606.ENSP00000404179.

PTM databases

iPTMnetiQ8N1I0.
PhosphoSitePlusiQ8N1I0.

Polymorphism and mutation databases

BioMutaiDOCK4.
DMDMi296439369.

Proteomic databases

EPDiQ8N1I0.
MaxQBiQ8N1I0.
PaxDbiQ8N1I0.
PeptideAtlasiQ8N1I0.
PRIDEiQ8N1I0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000428084; ENSP00000410746; ENSG00000128512. [Q8N1I0-3]
ENST00000437633; ENSP00000404179; ENSG00000128512. [Q8N1I0-1]
GeneIDi9732.
KEGGihsa:9732.
UCSCiuc003vfx.4. human. [Q8N1I0-1]

Organism-specific databases

CTDi9732.
DisGeNETi9732.
GeneCardsiDOCK4.
H-InvDBHIX0007003.
HGNCiHGNC:19192. DOCK4.
MIMi607679. gene.
neXtProtiNX_Q8N1I0.
OpenTargetsiENSG00000128512.
PharmGKBiPA134939318.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1998. Eukaryota.
ENOG410XQH7. LUCA.
GeneTreeiENSGT00610000085806.
HOGENOMiHOG000006631.
HOVERGENiHBG051389.
InParanoidiQ8N1I0.
KOiK17697.
PhylomeDBiQ8N1I0.
TreeFamiTF300423.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000128512-MONOMER.
ReactomeiR-HSA-983231. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

ChiTaRSiDOCK4. human.
GeneWikiiDock4.
GenomeRNAii9732.
PROiQ8N1I0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000128512.
CleanExiHS_DOCK4.
ExpressionAtlasiQ8N1I0. baseline and differential.
GenevisibleiQ8N1I0. HS.

Family and domain databases

InterProiIPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR010703. DOCK_C.
IPR032376. DOCK_N.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23317. PTHR23317. 1 hit.
PfamiPF06920. DHR-2. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF16172. DOCK_N. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDOCK4_HUMAN
AccessioniPrimary (citable) accession number: Q8N1I0
Secondary accession number(s): O14584, O94824, Q8NB45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 3, 2003
Last sequence update: May 18, 2010
Last modified: November 2, 2016
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Injection of wild-type protein suppresses tumor invasion in osteosarcoma mouse cell lines while it is not the case with the Leu-1718 variant.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.