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Protein

Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1

Gene

CMTR1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that mediates mRNA cap1 2'-O-ribose methylation to the 5'-cap structure of mRNAs. Methylates the ribose of the first nucleotide of a m7GpppG-capped mRNA and small nuclear RNA (snRNA) to produce m7GpppRm (cap1). Displays a preference for cap0 transcripts. Cap1 modification is linked to higher levels of translation. May be involved in the interferon response pathway.3 Publications

Catalytic activityi

S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-[mRNA].1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei281S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1
Binding sitei311S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei364S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei404Proton acceptor1 Publication1

GO - Molecular functioni

GO - Biological processi

  • 7-methylguanosine mRNA capping Source: UniProtKB
  • cap1 mRNA methylation Source: UniProtKB
  • mRNA methylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000137200-MONOMER.
ZFISH:ENSG00000137200-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 (EC:2.1.1.57)
Alternative name(s):
Cap methyltransferase 1
Cap1 2'O-ribose methyltransferase 1
Short name:
MTr1
Short name:
hMTr1
FtsJ methyltransferase domain-containing protein 2
Interferon-stimulated gene 95 kDa protein
Short name:
ISG95
Gene namesi
Name:CMTR1
Synonyms:FTSJD2, KIAA0082, MTR1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:21077. CMTR1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi239K → A: Abolishes catalytic activity. 1 Publication1
Mutagenesisi364D → A: Abolishes catalytic activity. 1 Publication1
Mutagenesisi404K → A: Abolishes catalytic activity. 1 Publication1

Organism-specific databases

DisGeNETi23070.
OpenTargetsiENSG00000137200.
PharmGKBiPA162389052.

Polymorphism and mutation databases

BioMutaiCMTR1.
DMDMi74750894.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002512391 – 835Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1Add BLAST835

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei28PhosphoserineBy similarity1
Modified residuei31PhosphoserineBy similarity1
Modified residuei53PhosphoserineCombined sources1
Modified residuei66PhosphoserineCombined sources1
Modified residuei91PhosphoserineCombined sources1
Modified residuei108N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8N1G2.
MaxQBiQ8N1G2.
PaxDbiQ8N1G2.
PeptideAtlasiQ8N1G2.
PRIDEiQ8N1G2.
TopDownProteomicsiQ8N1G2.

PTM databases

iPTMnetiQ8N1G2.
PhosphoSitePlusiQ8N1G2.

Expressioni

Inductioni

By interferons alpha and beta, and by Vaccinia virus infection.3 Publications

Gene expression databases

BgeeiENSG00000137200.
CleanExiHS_FTSJD2.
ExpressionAtlasiQ8N1G2. baseline and differential.
GenevisibleiQ8N1G2. HS.

Organism-specific databases

HPAiHPA029954.
HPA029979.
HPA029980.

Interactioni

Subunit structurei

Interacts with POLR2A (via C-terminus).1 Publication

Protein-protein interaction databases

BioGridi116703. 14 interactors.
IntActiQ8N1G2. 4 interactors.
STRINGi9606.ENSP00000362550.

Structurei

Secondary structure

1835
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi143 – 146Combined sources4
Turni168 – 174Combined sources7
Beta strandi176 – 179Combined sources4
Helixi184 – 186Combined sources3
Beta strandi189 – 191Combined sources3
Helixi193 – 204Combined sources12
Helixi205 – 208Combined sources4
Helixi211 – 221Combined sources11
Helixi225 – 227Combined sources3
Turni228 – 231Combined sources4
Beta strandi232 – 234Combined sources3
Helixi235 – 246Combined sources12
Turni247 – 252Combined sources6
Helixi265 – 267Combined sources3
Beta strandi271 – 277Combined sources7
Helixi282 – 291Combined sources10
Beta strandi293 – 300Combined sources8
Helixi310 – 312Combined sources3
Turni313 – 316Combined sources4
Turni318 – 320Combined sources3
Beta strandi321 – 323Combined sources3
Beta strandi326 – 328Combined sources3
Helixi329 – 331Combined sources3
Helixi339 – 351Combined sources13
Turni352 – 355Combined sources4
Beta strandi358 – 363Combined sources6
Turni370 – 372Combined sources3
Helixi373 – 375Combined sources3
Helixi376 – 379Combined sources4
Helixi381 – 394Combined sources14
Beta strandi395 – 405Combined sources11
Helixi411 – 423Combined sources13
Beta strandi424 – 430Combined sources7
Beta strandi442 – 449Combined sources8
Helixi454 – 470Combined sources17
Beta strandi473 – 481Combined sources9
Helixi483 – 487Combined sources5
Helixi490 – 519Combined sources30
Helixi528 – 539Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4N48X-ray2.70A/B126-550[»]
4N49X-ray1.90A126-550[»]
4N4AX-ray2.35A126-550[»]
ProteinModelPortaliQ8N1G2.
SMRiQ8N1G2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini87 – 133G-patchPROSITE-ProRule annotationAdd BLAST47
Domaini231 – 450RrmJ-type SAM-dependent 2'-O-MTasePROSITE-ProRule annotationAdd BLAST220
Domaini752 – 786WWAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni727 – 835Interaction with POLR2A1 PublicationAdd BLAST109

Sequence similaritiesi

Contains 1 G-patch domain.PROSITE-ProRule annotation
Contains 1 RrmJ-type SAM-dependent 2'-O-MTase domain.PROSITE-ProRule annotation
Contains 1 WW domain.Curated

Phylogenomic databases

eggNOGiKOG3673. Eukaryota.
ENOG410XP08. LUCA.
GeneTreeiENSGT00530000063742.
HOGENOMiHOG000021970.
HOVERGENiHBG057271.
KOiK14589.
OMAiSRLFWEW.
OrthoDBiEOG091G02K5.
PhylomeDBiQ8N1G2.
TreeFamiTF314897.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR030376. Cap_mRNA_MeTrfase_1.
IPR000467. G_patch_dom.
IPR025816. RrmJ-type_MeTrfase.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
IPR001202. WW_dom.
[Graphical view]
PANTHERiPTHR16121:SF0. PTHR16121:SF0. 1 hit.
PfamiPF01728. FtsJ. 1 hit.
PF01585. G-patch. 1 hit.
[Graphical view]
SMARTiSM00443. G_patch. 1 hit.
SM00456. WW. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS50174. G_PATCH. 1 hit.
PS51613. SAM_MT_RRMJ. 1 hit.
PS01159. WW_DOMAIN_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8N1G2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRRTDPECT APIKKQKKRV AELALSLSST SDDEPPSSVS HGAKASTTSL
60 70 80 90 100
SGSDSETEGK QHSSDSFDDA FKADSLVEGT SSRYSMYNSV SQKLMAKMGF
110 120 130 140 150
REGEGLGKYS QGRKDIVEAS SQKGRRGLGL TLRGFDQELN VDWRDEPEPS
160 170 180 190 200
ACEQVSWFPE CTTEIPDTQE MSDWMVVGKR KMIIEDETEF CGEELLHSVL
210 220 230 240 250
QCKSVFDVLD GEEMRRARTR ANPYEMIRGV FFLNRAAMKM ANMDFVFDRM
260 270 280 290 300
FTNPRDSYGK PLVKDREAEL LYFADVCAGP GGFSEYVLWR KKWHAKGFGM
310 320 330 340 350
TLKGPNDFKL EDFYSASSEL FEPYYGEGGI DGDGDITRPE NISAFRNFVL
360 370 380 390 400
DNTDRKGVHF LMADGGFSVE GQENLQEILS KQLLLCQFLM ALSIVRTGGH
410 420 430 440 450
FICKTFDLFT PFSVGLVYLL YCCFERVCLF KPITSRPANS ERYVVCKGLK
460 470 480 490 500
VGIDDVRDYL FAVNIKLNQL RNTDSDVNLV VPLEVIKGDH EFTDYMIRSN
510 520 530 540 550
ESHCSLQIKA LAKIHAFVQD TTLSEPRQAE IRKECLRLWG IPDQARVAPS
560 570 580 590 600
SSDPKSKFFE LIQGTEIDIF SYKPTLLTSK TLEKIRPVFD YRCMVSGSEQ
610 620 630 640 650
KFLIGLGKSQ IYTWDGRQSD RWIKLDLKTE LPRDTLLSVE IVHELKGEGK
660 670 680 690 700
AQRKISAIHI LDVLVLNGTD VREQHFNQRI QLAEKFVKAV SKPSRPDMNP
710 720 730 740 750
IRVKEVYRLE EMEKIFVRLE MKIIKGSSGT PKLSYTGRDD RHFVPMGLYI
760 770 780 790 800
VRTVNEPWTM GFSKSFKKKF FYNKKTKDST FDLPADSIAP FHICYYGRLF
810 820 830
WEWGDGIRVH DSQKPQDQDK LSKEDVLSFI QMHRA
Length:835
Mass (Da):95,321
Last modified:October 1, 2002 - v1
Checksum:i03919512A73C30FB
GO

Sequence cautioni

The sequence BAA07893 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti795Y → H in BAF85253 (PubMed:14702039).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D43949 mRNA. Translation: BAA07893.2. Different initiation.
AK292564 mRNA. Translation: BAF85253.1.
AL353597 Genomic DNA. Translation: CAI19603.1.
BC010731 mRNA. Translation: AAH10731.2.
BC031890 mRNA. Translation: AAH31890.1.
CCDSiCCDS4835.1.
RefSeqiNP_055865.1. NM_015050.2.
UniGeneiHs.520102.

Genome annotation databases

EnsembliENST00000373451; ENSP00000362550; ENSG00000137200.
GeneIDi23070.
KEGGihsa:23070.
UCSCiuc003ons.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D43949 mRNA. Translation: BAA07893.2. Different initiation.
AK292564 mRNA. Translation: BAF85253.1.
AL353597 Genomic DNA. Translation: CAI19603.1.
BC010731 mRNA. Translation: AAH10731.2.
BC031890 mRNA. Translation: AAH31890.1.
CCDSiCCDS4835.1.
RefSeqiNP_055865.1. NM_015050.2.
UniGeneiHs.520102.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4N48X-ray2.70A/B126-550[»]
4N49X-ray1.90A126-550[»]
4N4AX-ray2.35A126-550[»]
ProteinModelPortaliQ8N1G2.
SMRiQ8N1G2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116703. 14 interactors.
IntActiQ8N1G2. 4 interactors.
STRINGi9606.ENSP00000362550.

PTM databases

iPTMnetiQ8N1G2.
PhosphoSitePlusiQ8N1G2.

Polymorphism and mutation databases

BioMutaiCMTR1.
DMDMi74750894.

Proteomic databases

EPDiQ8N1G2.
MaxQBiQ8N1G2.
PaxDbiQ8N1G2.
PeptideAtlasiQ8N1G2.
PRIDEiQ8N1G2.
TopDownProteomicsiQ8N1G2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373451; ENSP00000362550; ENSG00000137200.
GeneIDi23070.
KEGGihsa:23070.
UCSCiuc003ons.4. human.

Organism-specific databases

CTDi23070.
DisGeNETi23070.
GeneCardsiCMTR1.
HGNCiHGNC:21077. CMTR1.
HPAiHPA029954.
HPA029979.
HPA029980.
MIMi616189. gene.
neXtProtiNX_Q8N1G2.
OpenTargetsiENSG00000137200.
PharmGKBiPA162389052.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3673. Eukaryota.
ENOG410XP08. LUCA.
GeneTreeiENSGT00530000063742.
HOGENOMiHOG000021970.
HOVERGENiHBG057271.
KOiK14589.
OMAiSRLFWEW.
OrthoDBiEOG091G02K5.
PhylomeDBiQ8N1G2.
TreeFamiTF314897.

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000137200-MONOMER.
ZFISH:ENSG00000137200-MONOMER.

Miscellaneous databases

GenomeRNAii23070.
PROiQ8N1G2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000137200.
CleanExiHS_FTSJD2.
ExpressionAtlasiQ8N1G2. baseline and differential.
GenevisibleiQ8N1G2. HS.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR030376. Cap_mRNA_MeTrfase_1.
IPR000467. G_patch_dom.
IPR025816. RrmJ-type_MeTrfase.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
IPR001202. WW_dom.
[Graphical view]
PANTHERiPTHR16121:SF0. PTHR16121:SF0. 1 hit.
PfamiPF01728. FtsJ. 1 hit.
PF01585. G-patch. 1 hit.
[Graphical view]
SMARTiSM00443. G_patch. 1 hit.
SM00456. WW. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS50174. G_PATCH. 1 hit.
PS51613. SAM_MT_RRMJ. 1 hit.
PS01159. WW_DOMAIN_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCMTR1_HUMAN
AccessioniPrimary (citable) accession number: Q8N1G2
Secondary accession number(s): A8K949, Q14670, Q96FJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.