Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vacuolar protein sorting-associated protein 52 homolog

Gene

VPS52

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD (PubMed:15878329, PubMed:18367545). Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane (PubMed:25799061).3 Publications

GO - Molecular functioni

  • Rab GTPase binding Source: GO_Central
  • syntaxin binding Source: UniProtKB

GO - Biological processi

  • ectodermal cell differentiation Source: Ensembl
  • embryonic ectodermal digestive tract development Source: Ensembl
  • endocytic recycling Source: UniProtKB
  • Golgi to vacuole transport Source: GO_Central
  • lysosomal transport Source: MGI
  • protein transport Source: UniProtKB-KW
  • retrograde transport, endosome to Golgi Source: GO_Central

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-6811440 Retrograde transport at the Trans-Golgi-Network

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 52 homolog
Alternative name(s):
SAC2 suppressor of actin mutations 2-like protein
Gene namesi
Name:VPS52
Synonyms:SACM2L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000223501.8
HGNCiHGNC:10518 VPS52
MIMi603443 gene
neXtProtiNX_Q8N1B4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi6293
OpenTargetsiENSG00000223501
PharmGKBiPA34926

Polymorphism and mutation databases

BioMutaiVPS52
DMDMi74750887

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002133152 – 723Vacuolar protein sorting-associated protein 52 homologAdd BLAST722

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei355PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8N1B4
MaxQBiQ8N1B4
PaxDbiQ8N1B4
PeptideAtlasiQ8N1B4
PRIDEiQ8N1B4
ProteomicsDBi71582

PTM databases

iPTMnetiQ8N1B4
PhosphoSitePlusiQ8N1B4

Expressioni

Gene expression databases

BgeeiENSG00000223501
CleanExiHS_VPS52
ExpressionAtlasiQ8N1B4 baseline and differential
GenevisibleiQ8N1B4 HS

Organism-specific databases

HPAiHPA042667
HPA048128

Interactioni

Subunit structurei

Component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54 (PubMed:15878329, PubMed:20685960). Component of the endosome-associated retrograde protein (EARP) complex, composed of VPS51, VPS52, VPS53 and VPS50/Syndetin (PubMed:25799061). Interacts with RAB6A and STX10 (PubMed:15878329, PubMed:20685960). Interacts with UHRF1BP1L (PubMed:20163565).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Q9WMX22EBI-2799833,EBI-6863748From Hepatitis C virus genotype 1b (isolate Con1).
AAMPC9JG973EBI-2799833,EBI-10176499
ATP6V1DQ6PJ053EBI-2799833,EBI-10253919
ATP6V1DQ8N5Z93EBI-2799833,EBI-10266952
ATP6V1DQ9Y5K85EBI-2799833,EBI-2684998
C1orf109Q9NX045EBI-2799833,EBI-8643161
CCDC146Q8IYE0-23EBI-2799833,EBI-10247802
DCXO43602-24EBI-2799833,EBI-14148644
DDX6P261965EBI-2799833,EBI-351257
EPM2AIP1Q7L7755EBI-2799833,EBI-6255981
FAM110AQ9BQ894EBI-2799833,EBI-1752811
FAM184AQ6P9G83EBI-2799833,EBI-10253239
GEMP550404EBI-2799833,EBI-744104
GFM2Q969S93EBI-2799833,EBI-2371750
GOLGA1Q928055EBI-2799833,EBI-6164177
HAUS1Q96CS23EBI-2799833,EBI-2514791
HEATR1Q96ES53EBI-2799833,EBI-10285245
HEL-S-61V9HW293EBI-2799833,EBI-10330057
HGSA0A0S2Z4Q43EBI-2799833,EBI-16429135
HGSO149643EBI-2799833,EBI-740220
KANK2Q63ZY33EBI-2799833,EBI-2556193
KIAA1217Q5T5P2-63EBI-2799833,EBI-10188326
KLC3Q6P5973EBI-2799833,EBI-1643885
LMO4P619683EBI-2799833,EBI-2798728
METTL13Q8N6R03EBI-2799833,EBI-1053295
MFAP1P550815EBI-2799833,EBI-1048159
MRPL11Q9Y3B73EBI-2799833,EBI-5453723
NFKBIBQ156533EBI-2799833,EBI-352889
NOP2P460873EBI-2799833,EBI-356811
PIMREGQ9BSJ63EBI-2799833,EBI-2568609
PPP1R18Q6NYC85EBI-2799833,EBI-2557469
PRKAA1Q131313EBI-2799833,EBI-1181405
PRKAA2P546463EBI-2799833,EBI-1383852
RAB4AP203383EBI-2799833,EBI-722284
RNF41Q9H4P45EBI-2799833,EBI-2130266
SH2D4AQ9H7883EBI-2799833,EBI-747035
STX11O755583EBI-2799833,EBI-714135
TBC1D22BQ9NU195EBI-2799833,EBI-8787464
TCEANCQ8N8B73EBI-2799833,EBI-954696
TEAD4D3DUQ63EBI-2799833,EBI-10176734
TEAD4Q155613EBI-2799833,EBI-747736
THAP11Q96EK43EBI-2799833,EBI-1790529
TPM3P067533EBI-2799833,EBI-355607
TPM3Q5VU623EBI-2799833,EBI-10184033
TRAF6Q9Y4K35EBI-2799833,EBI-359276
TSSK3Q96PN87EBI-2799833,EBI-3918381
TSTD2Q5T7W75EBI-2799833,EBI-8994397
TXLNAP402225EBI-2799833,EBI-359793
TXN2Q997575EBI-2799833,EBI-2932492
USP2O756045EBI-2799833,EBI-743272
VPS28Q548N13EBI-2799833,EBI-10243107
VPS28Q9UK414EBI-2799833,EBI-727424
VPS50Q96JG67EBI-2799833,EBI-11044388
VPS51Q9UID3-14EBI-2799833,EBI-16067837
WTAPQ150075EBI-2799833,EBI-751647
ZMAT2Q96NC03EBI-2799833,EBI-2682299

GO - Molecular functioni

  • Rab GTPase binding Source: GO_Central
  • syntaxin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi112200, 77 interactors
CORUMiQ8N1B4
DIPiDIP-57843N
IntActiQ8N1B4, 128 interactors
MINTiQ8N1B4
STRINGi9606.ENSP00000409952

Structurei

3D structure databases

ProteinModelPortaliQ8N1B4
SMRiQ8N1B4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili107 – 127Sequence analysisAdd BLAST21
Coiled coili194 – 215Sequence analysisAdd BLAST22

Sequence similaritiesi

Belongs to the VPS52 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1961 Eukaryota
ENOG410XNZ1 LUCA
GeneTreeiENSGT00390000008815
HOVERGENiHBG052966
InParanoidiQ8N1B4
KOiK20298
OMAiYKFRKPM
OrthoDBiEOG09370VL2
PhylomeDBiQ8N1B4
TreeFamiTF314937

Family and domain databases

InterProiView protein in InterPro
IPR007258 Vps52
PANTHERiPTHR14190 PTHR14190, 1 hit
PfamiView protein in Pfam
PF04129 Vps52, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N1B4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAATMAAA ARELVLRAGT SDMEEEEGPL AGGPGLQEPL QLGELDITSD
60 70 80 90 100
EFILDEVDVH IQANLEDELV KEALKTGVDL RHYSKQVELE LQQIEQKSIR
110 120 130 140 150
DYIQESENIA SLHNQITACD AVLERMEQML GAFQSDLSSI SSEIRTLQEQ
160 170 180 190 200
SGAMNIRLRN RQAVRGKLGE LVDGLVVPSA LVTAILEAPV TEPRFLEQLQ
210 220 230 240 250
ELDAKAAAVR EQEARGTAAC ADVRGVLDRL RVKAVTKIRE FILQKIYSFR
260 270 280 290 300
KPMTNYQIPQ TALLKYRFFY QFLLGNERAT AKEIRDEYVE TLSKIYLSYY
310 320 330 340 350
RSYLGRLMKV QYEEVAEKDD LMGVEDTAKK GFFSKPSLRS RNTIFTLGTR
360 370 380 390 400
GSVISPTELE APILVPHTAQ RGEQRYPFEA LFRSQHYALL DNSCREYLFI
410 420 430 440 450
CEFFVVSGPA AHDLFHAVMG RTLSMTLKHL DSYLADCYDA IAVFLCIHIV
460 470 480 490 500
LRFRNIAAKR DVPALDRYWE QVLALLWPRF ELILEMNVQS VRSTDPQRLG
510 520 530 540 550
GLDTRPHYIT RRYAEFSSAL VSINQTIPNE RTMQLLGQLQ VEVENFVLRV
560 570 580 590 600
AAEFSSRKEQ LVFLINNYDM MLGVLMERAA DDSKEVESFQ QLLNARTQEF
610 620 630 640 650
IEELLSPPFG GLVAFVKEAE ALIERGQAER LRGEEARVTQ LIRGFGSSWK
660 670 680 690 700
SSVESLSQDV MRSFTNFRNG TSIIQGALTQ LIQLYHRFHR VLSQPQLRAL
710 720
PARAELINIH HLMVELKKHK PNF
Length:723
Mass (Da):82,221
Last modified:October 1, 2002 - v1
Checksum:iC0961E2BE50B24DF
GO
Isoform 2 (identifier: Q8N1B4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-125: Missing.

Note: No experimental confirmation available.
Show »
Length:598
Mass (Da):68,496
Checksum:iA5901265E5EEDBC7
GO

Sequence cautioni

The sequence CAI95619 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti710H → P in BAD96587 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0564761 – 125Missing in isoform 2. 1 PublicationAdd BLAST125

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK297935 mRNA Translation: BAG60251.1
AK222867 mRNA Translation: BAD96587.1
AL645940 Genomic DNA No translation available.
AL031228 Genomic DNA Translation: CAI95619.1 Sequence problems.
BX248408 Genomic DNA Translation: CAM26258.1
CR762434, CR759817 Genomic DNA Translation: CAQ06872.1
CR759817, CR762434 Genomic DNA Translation: CAQ08020.1
CR759786 Genomic DNA Translation: CAQ08250.1
CH471081 Genomic DNA Translation: EAX03688.1
BC032108 mRNA Translation: AAH32108.1
BC040114 mRNA Translation: AAH40114.2
AL390171 mRNA Translation: CAB99099.1
CCDSiCCDS4770.2 [Q8N1B4-1]
PIRiT51882
RefSeqiNP_001276104.1, NM_001289175.1 [Q8N1B4-2]
NP_001276105.1, NM_001289176.1
NP_072047.4, NM_022553.5 [Q8N1B4-1]
XP_016866667.1, XM_017011178.1 [Q8N1B4-2]
UniGeneiHs.480356

Genome annotation databases

EnsembliENST00000383210; ENSP00000372697; ENSG00000206286 [Q8N1B4-1]
ENST00000428608; ENSP00000406988; ENSG00000236014 [Q8N1B4-1]
ENST00000441058; ENSP00000390831; ENSG00000228425 [Q8N1B4-1]
ENST00000443860; ENSP00000404016; ENSG00000224455 [Q8N1B4-1]
ENST00000445902; ENSP00000409952; ENSG00000223501 [Q8N1B4-1]
ENST00000448042; ENSP00000391197; ENSG00000225590 [Q8N1B4-1]
GeneIDi6293
KEGGihsa:6293
UCSCiuc003odm.3 human [Q8N1B4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiVPS52_HUMAN
AccessioniPrimary (citable) accession number: Q8N1B4
Secondary accession number(s): A2BF38
, B0UZZ4, B4DNI9, Q53GR4, Q5JPA0, Q5SQW1, Q8IUN6, Q9NPT5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: October 1, 2002
Last modified: June 20, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health