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Protein

Sodium-dependent phosphate transport protein 2C

Gene

SLC34A3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in actively transporting phosphate into cells via Na+ cotransport in the renal brush border membrane. Probably mediates 20-30% of the apical influx.

Kineticsi

  1. KM=79 µM for phosphate1 Publication

    GO - Molecular functioni

    • sodium:phosphate symporter activity Source: UniProtKB
    • sodium-dependent phosphate transmembrane transporter activity Source: UniProtKB

    GO - Biological processi

    • cellular phosphate ion homeostasis Source: UniProtKB
    • ion transport Source: Reactome
    • phosphate ion transmembrane transport Source: GOC
    • phosphate ion transport Source: UniProtKB
    • sodium ion transmembrane transport Source: GOC
    • sodium ion transport Source: UniProtKB
    • transmembrane transport Source: Reactome
    Complete GO annotation...

    Keywords - Biological processi

    Ion transport, Sodium transport, Symport, Transport

    Keywords - Ligandi

    Sodium

    Enzyme and pathway databases

    ReactomeiREACT_19411. Type II Na+/Pi cotransporters.

    Protein family/group databases

    TCDBi2.A.58.1.3. the phosphate:na(+) symporter (pnas) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium-dependent phosphate transport protein 2C
    Short name:
    Sodium-phosphate transport protein 2C
    Alternative name(s):
    Na(+)-dependent phosphate cotransporter 2C
    Sodium/inorganic phosphate cotransporter IIC
    Sodium/phosphate cotransporter 2C
    Short name:
    Na(+)/Pi cotransporter 2C
    Short name:
    NaPi-2c
    Solute carrier family 34 member 3
    Gene namesi
    Name:SLC34A3
    Synonyms:NPT2C, NPTIIC
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640 Componenti: Chromosome 9

    Organism-specific databases

    HGNCiHGNC:20305. SLC34A3.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 7676CytoplasmicSequence AnalysisAdd
    BLAST
    Transmembranei77 – 9721Helical; Name=M1Sequence AnalysisAdd
    BLAST
    Topological domaini98 – 11114ExtracellularSequence AnalysisAdd
    BLAST
    Transmembranei112 – 13221Helical; Name=M2Sequence AnalysisAdd
    BLAST
    Topological domaini133 – 18856CytoplasmicSequence AnalysisAdd
    BLAST
    Transmembranei189 – 20921Helical; Name=M3Sequence AnalysisAdd
    BLAST
    Topological domaini210 – 322113ExtracellularSequence AnalysisAdd
    BLAST
    Transmembranei323 – 34321Helical; Name=M4Sequence AnalysisAdd
    BLAST
    Topological domaini344 – 36724CytoplasmicSequence AnalysisAdd
    BLAST
    Transmembranei368 – 38821Helical; Name=M5Sequence AnalysisAdd
    BLAST
    Topological domaini389 – 44557ExtracellularSequence AnalysisAdd
    BLAST
    Transmembranei446 – 46621Helical; Name=M6Sequence AnalysisAdd
    BLAST
    Topological domaini467 – 48519CytoplasmicSequence AnalysisAdd
    BLAST
    Transmembranei486 – 50621Helical; Name=M7Sequence AnalysisAdd
    BLAST
    Topological domaini507 – 5104ExtracellularSequence Analysis
    Transmembranei511 – 53121Helical; Name=M8Sequence AnalysisAdd
    BLAST
    Topological domaini532 – 59968CytoplasmicSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    • apical plasma membrane Source: HGNC
    • brush border membrane Source: InterPro
    • integral component of membrane Source: UniProtKB-KW
    • plasma membrane Source: Reactome
    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Involvement in diseasei

    Hereditary hypophosphatemic rickets with hypercalciuria (HHRH)2 Publications

    The disease is caused by mutations affecting the gene represented in this entry.

    Disease descriptionAutosomal recessive form of hypophosphatemia characterized by reduced renal phosphate reabsorption and rickets. Increased serum levels of 1,25-dihydroxyvitamin D lead to increase in urinary calcium excretion.

    See also OMIM:241530
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti138 – 1381S → F in HHRH. 1 Publication
    VAR_025707
    Natural varianti192 – 1921S → L in HHRH. 2 Publications
    VAR_025709
    Natural varianti196 – 1961G → R in HHRH. 1 Publication
    VAR_025710
    Natural varianti353 – 3531R → L in HHRH. 1 Publication
    VAR_025713
    Natural varianti413 – 4131A → E in HHRH. 1 Publication
    VAR_025714
    Natural varianti468 – 4681R → W in HHRH. 1 Publication
    VAR_025715

    Keywords - Diseasei

    Disease mutation

    Organism-specific databases

    MIMi241530. phenotype.
    Orphaneti157215. Hereditary hypophosphatemic rickets with hypercalciuria.
    PharmGKBiPA134930298.

    Polymorphism and mutation databases

    BioMutaiSLC34A3.
    DMDMi313104149.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 599599Sodium-dependent phosphate transport protein 2CPRO_0000068617Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi265 – 2651N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi268 – 2681N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi276 ↔ 309By similarity
    Glycosylationi286 – 2861N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi299 – 2991N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ8N130.
    PRIDEiQ8N130.

    PTM databases

    PhosphoSiteiQ8N130.

    Expressioni

    Gene expression databases

    BgeeiQ8N130.
    CleanExiHS_SLC34A3.
    GenevisibleiQ8N130. HS.

    Organism-specific databases

    HPAiHPA023776.

    Interactioni

    Protein-protein interaction databases

    BioGridi126771. 2 interactions.
    STRINGi9606.ENSP00000355353.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8N130.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi424 – 4274Poly-Thr

    Sequence similaritiesi

    Belongs to the SLC34A transporter family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1283.
    GeneTreeiENSGT00390000005032.
    HOGENOMiHOG000006550.
    HOVERGENiHBG006527.
    InParanoidiQ8N130.
    KOiK14683.
    OMAiLIKRWCG.
    OrthoDBiEOG72ZCDP.
    PhylomeDBiQ8N130.
    TreeFamiTF313981.

    Family and domain databases

    InterProiIPR003841. Na/Pi_transpt.
    IPR029850. Na/Pi_transpt_2C.
    [Graphical view]
    PANTHERiPTHR10010:SF22. PTHR10010:SF22. 1 hit.
    PfamiPF02690. Na_Pi_cotrans. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR01013. 2a58. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8N130-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPSSLPGSQV PHPTLDAVDL VEKTLRNEGT SSSAPVLEEG DTDPWTLPQL
    60 70 80 90 100
    KDTSQPWKEL RVAGRLRRVA GSVLKACGLL GSLYFFICSL DVLSSAFQLL
    110 120 130 140 150
    GSKVAGDIFK DNVVLSNPVA GLVIGVLVTA LVQSSSTSSS IVVSMVAAKL
    160 170 180 190 200
    LTVRVSVPII MGVNVGTSIT STLVSMAQSG DRDEFQRAFS GSAVHGIFNW
    210 220 230 240 250
    LTVLVLLPLE SATALLERLS ELALGAASLT PRAQAPDILK VLTKPLTHLI
    260 270 280 290 300
    VQLDSDMIMS SATGNATNSS LIKHWCGTTG QPTQENSSCG AFGPCTEKNS
    310 320 330 340 350
    TAPADRLPCR HLFAGTELTD LAVGCILLAG SLLVLCGCLV LIVKLLNSVL
    360 370 380 390 400
    RGRVAQVVRT VINADFPFPL GWLGGYLAVL AGAGLTFALQ SSSVFTAAVV
    410 420 430 440 450
    PLMGVGVISL DRAYPLLLGS NIGTTTTALL AALASPADRM LSALQVALIH
    460 470 480 490 500
    FFFNLAGILL WYLVPALRLP IPLARHFGVV TARYRWVAGV YLLLGFLLLP
    510 520 530 540 550
    LAAFGLSLAG GMELAAVGGP LVGLVLLVIL VTVLQRRRPA WLPVRLRSWA
    560 570 580 590
    WLPVWLHSLE PWDRLVTRCC PCNVCSPPKA TTKEAYCYEN PEILASQQL
    Length:599
    Mass (Da):63,550
    Last modified:November 30, 2010 - v2
    Checksum:i3747DE33D0A53E95
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti67 – 671R → H.1 Publication
    Corresponds to variant rs34372115 [ dbSNP | Ensembl ].
    VAR_025706
    Natural varianti138 – 1381S → F in HHRH. 1 Publication
    VAR_025707
    Natural varianti180 – 1801G → A.1 Publication
    Corresponds to variant rs35643193 [ dbSNP | Ensembl ].
    VAR_025708
    Natural varianti192 – 1921S → L in HHRH. 2 Publications
    VAR_025709
    Natural varianti196 – 1961G → R in HHRH. 1 Publication
    VAR_025710
    Natural varianti237 – 2371D → N.1 Publication
    Corresponds to variant rs145877051 [ dbSNP | Ensembl ].
    VAR_025711
    Natural varianti337 – 3371G → S.1 Publication
    Corresponds to variant rs35699762 [ dbSNP | Ensembl ].
    VAR_025712
    Natural varianti353 – 3531R → L in HHRH. 1 Publication
    VAR_025713
    Natural varianti413 – 4131A → E in HHRH. 1 Publication
    VAR_025714
    Natural varianti468 – 4681R → W in HHRH. 1 Publication
    VAR_025715
    Natural varianti513 – 5131E → V.4 Publications
    Corresponds to variant rs28542318 [ dbSNP | Ensembl ].
    VAR_025716

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB055000 mRNA. Translation: BAB83242.1.
    AK095999 mRNA. Translation: BAC04667.1.
    BX255925 Genomic DNA. Translation: CAM24147.1.
    CCDSiCCDS7038.1.
    RefSeqiNP_001170787.1. NM_001177316.1.
    NP_001170788.1. NM_001177317.1.
    NP_543153.1. NM_080877.2.
    UniGeneiHs.432442.

    Genome annotation databases

    EnsembliENST00000361134; ENSP00000355353; ENSG00000198569.
    ENST00000538474; ENSP00000442397; ENSG00000198569.
    GeneIDi142680.
    KEGGihsa:142680.
    UCSCiuc004cmf.1. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB055000 mRNA. Translation: BAB83242.1.
    AK095999 mRNA. Translation: BAC04667.1.
    BX255925 Genomic DNA. Translation: CAM24147.1.
    CCDSiCCDS7038.1.
    RefSeqiNP_001170787.1. NM_001177316.1.
    NP_001170788.1. NM_001177317.1.
    NP_543153.1. NM_080877.2.
    UniGeneiHs.432442.

    3D structure databases

    ProteinModelPortaliQ8N130.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi126771. 2 interactions.
    STRINGi9606.ENSP00000355353.

    Protein family/group databases

    TCDBi2.A.58.1.3. the phosphate:na(+) symporter (pnas) family.

    PTM databases

    PhosphoSiteiQ8N130.

    Polymorphism and mutation databases

    BioMutaiSLC34A3.
    DMDMi313104149.

    Proteomic databases

    PaxDbiQ8N130.
    PRIDEiQ8N130.

    Protocols and materials databases

    DNASUi142680.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000361134; ENSP00000355353; ENSG00000198569.
    ENST00000538474; ENSP00000442397; ENSG00000198569.
    GeneIDi142680.
    KEGGihsa:142680.
    UCSCiuc004cmf.1. human.

    Organism-specific databases

    CTDi142680.
    GeneCardsiGC09P140125.
    HGNCiHGNC:20305. SLC34A3.
    HPAiHPA023776.
    MIMi241530. phenotype.
    609826. gene.
    neXtProtiNX_Q8N130.
    Orphaneti157215. Hereditary hypophosphatemic rickets with hypercalciuria.
    PharmGKBiPA134930298.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiCOG1283.
    GeneTreeiENSGT00390000005032.
    HOGENOMiHOG000006550.
    HOVERGENiHBG006527.
    InParanoidiQ8N130.
    KOiK14683.
    OMAiLIKRWCG.
    OrthoDBiEOG72ZCDP.
    PhylomeDBiQ8N130.
    TreeFamiTF313981.

    Enzyme and pathway databases

    ReactomeiREACT_19411. Type II Na+/Pi cotransporters.

    Miscellaneous databases

    ChiTaRSiSLC34A3. human.
    GeneWikiiSLC34A3.
    GenomeRNAii142680.
    NextBioi84599.
    PROiQ8N130.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ8N130.
    CleanExiHS_SLC34A3.
    GenevisibleiQ8N130. HS.

    Family and domain databases

    InterProiIPR003841. Na/Pi_transpt.
    IPR029850. Na/Pi_transpt_2C.
    [Graphical view]
    PANTHERiPTHR10010:SF22. PTHR10010:SF22. 1 hit.
    PfamiPF02690. Na_Pi_cotrans. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR01013. 2a58. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, BIOPHYSICOCHEMICAL PROPERTIES, VARIANT VAL-513.
      Tissue: Kidney.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-513.
      Tissue: Kidney.
    3. "DNA sequence and analysis of human chromosome 9."
      Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
      , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
      Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "SLC34A3 mutations in patients with hereditary hypophosphatemic rickets with hypercalciuria predict a key role for the sodium-phosphate cotransporter NaPi-IIc in maintaining phosphate homeostasis."
      Bergwitz C., Roslin N.M., Tieder M., Loredo-Osti J.C., Bastepe M., Abu-Zahra H., Frappier D., Burkett K., Carpenter T.O., Anderson D., Garabedian M., Sermet I., Fujiwara T.M., Morgan K., Tenenhouse H.S., Jueppner H.
      Am. J. Hum. Genet. 78:179-192(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS HHRH PHE-138; LEU-192; ARG-196 AND TRP-468, VARIANT VAL-513.
    5. "Hereditary hypophosphatemic rickets with hypercalciuria is caused by mutations in the sodium-phosphate cotransporter gene SLC34A3."
      Lorenz-Depiereux B., Benet-Pages A., Eckstein G., Tenenbaum-Rakover Y., Wagenstaller J., Tiosano D., Gershoni-Baruch R., Albers N., Lichtner P., Schnabel D., Hochberg Z., Strom T.M.
      Am. J. Hum. Genet. 78:193-201(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS HHRH LEU-192; LEU-353 AND GLU-413, VARIANTS HIS-67; ALA-180; ASN-237; SER-337 AND VAL-513.

    Entry informationi

    Entry nameiNPT2C_HUMAN
    AccessioniPrimary (citable) accession number: Q8N130
    Secondary accession number(s): A2BFA1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: November 30, 2010
    Last modified: June 24, 2015
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    A 2:1 stoichiometry to Na+/Pi is observed at pH 7.4.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 9
      Human chromosome 9: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.