Q8N118 (CP4X1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 4X1
EC=1.14.14.1

Alternative name(s):
CYPIVX1

Gene names

Name:	CYP4X1
ORF Names:	UNQ1929/PRO4404

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane By similarity; Single-pass membrane protein Potential. Microsome membrane By similarity.
Tissue specificity	Expressed in brain, heart and kidney and, at lower levels, in skeletal muscle and liver. In the heart, very high level levels in aorta, but very levels in other heart regions. Ref.1
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N118-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N118-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-59: MEFSWLETRW...PTHWFLGHQK → MMWGGGLDLC...ICMTSSSSGL

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8N118-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	Cytochrome P450 4X1		PRO_0000051862
Regions
	Transmembrane	14 – 34	21	Helical; Potential
Sites
	Metal binding	454	1	Iron (heme axial ligand) By similarity
Natural variations
	Alternative sequence	1 – 65	65	Missing in isoform 3.		VSP_045889
	Alternative sequence	1 – 59	59	MEFSW…LGHQK → MMWGGGLDLCPMPGQLKFPP CLSRCLLWEPPSLYLTQPTS SLAEPQALICMTSSSSGL in isoform 2.		VSP_045890
Experimental info
	Sequence conflict	192	1	I → V in BAD18508. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 702470A76E814278	FASTA	509	58,875
	10 20 30 40 50 60 MEFSWLETRW ARPFYLAFVF CLALGLLQAI KLYLRRQRLL RDLRPFPAPP THWFLGHQKF 70 80 90 100 110 120 IQDDNMEKLE EIIEKYPRAF PFWIGPFQAF FCIYDPDYAK TLLSRTDPKS QYLQKFSPPL 130 140 150 160 170 180 LGKGLAALDG PKWFQHRRLL TPGFHFNILK AYIEVMAHSV KMMLDKWEKI CSTQDTSVEV 190 200 210 220 230 240 YEHINSMSLD IIMKCAFSKE TNCQTNSTHD PYAKAIFELS KIIFHRLYSL LYHSDIIFKL 250 260 270 280 290 300 SPQGYRFQKL SRVLNQYTDT IIQERKKSLQ AGVKQDNTPK RKYQDFLDIV LSAKDESGSS 310 320 330 340 350 360 FSDIDVHSEV STFLLAGHDT LAASISWILY CLALNPEHQE RCREEVRGIL GDGSSITWDQ 370 380 390 400 410 420 LGEMSYTTMC IKETCRLIPA VPSISRDLSK PLTFPDGCTL PAGITVVLSI WGLHHNPAVW 430 440 450 460 470 480 KNPKVFDPLR FSQENSDQRH PYAYLPFSAG SRNCIGQEFA MIELKVTIAL ILLHFRVTPD 490 500 PTRPLTFPNH FILKPKNGMY LHLKKLSEC
	Isoform 2 [UniParc]. Checksum: 8E66A23B0353DC06	FASTA	508	57,901
	10 20 30 40 50 60 MMWGGGLDLC PMPGQLKFPP CLSRCLLWEP PSLYLTQPTS SLAEPQALIC MTSSSSGLFI 70 80 90 100 110 120 QDDNMEKLEE IIEKYPRAFP FWIGPFQAFF CIYDPDYAKT LLSRTDPKSQ YLQKFSPPLL 130 140 150 160 170 180 GKGLAALDGP KWFQHRRLLT PGFHFNILKA YIEVMAHSVK MMLDKWEKIC STQDTSVEVY 190 200 210 220 230 240 EHINSMSLDI IMKCAFSKET NCQTNSTHDP YAKAIFELSK IIFHRLYSLL YHSDIIFKLS 250 260 270 280 290 300 PQGYRFQKLS RVLNQYTDTI IQERKKSLQA GVKQDNTPKR KYQDFLDIVL SAKDESGSSF 310 320 330 340 350 360 SDIDVHSEVS TFLLAGHDTL AASISWILYC LALNPEHQER CREEVRGILG DGSSITWDQL 370 380 390 400 410 420 GEMSYTTMCI KETCRLIPAV PSISRDLSKP LTFPDGCTLP AGITVVLSIW GLHHNPAVWK 430 440 450 460 470 480 NPKVFDPLRF SQENSDQRHP YAYLPFSAGS RNCIGQEFAM IELKVTIALI LLHFRVTPDP 490 500 TRPLTFPNHF ILKPKNGMYL HLKKLSEC
	Isoform 3 [UniParc]. Checksum: 8162580C640B5813	FASTA	444	50,944
	10 20 30 40 50 60 MEKLEEIIEK YPRAFPFWIG PFQAFFCIYD PDYAKTLLSR TDPKSQYLQK FSPPLLGKGL 70 80 90 100 110 120 AALDGPKWFQ HRRLLTPGFH FNILKAYIEV MAHSVKMMLD KWEKICSTQD TSVEVYEHIN 130 140 150 160 170 180 SMSLDIIMKC AFSKETNCQT NSTHDPYAKA IFELSKIIFH RLYSLLYHSD IIFKLSPQGY 190 200 210 220 230 240 RFQKLSRVLN QYTDTIIQER KKSLQAGVKQ DNTPKRKYQD FLDIVLSAKD ESGSSFSDID 250 260 270 280 290 300 VHSEVSTFLL AGHDTLAASI SWILYCLALN PEHQERCREE VRGILGDGSS ITWDQLGEMS 310 320 330 340 350 360 YTTMCIKETC RLIPAVPSIS RDLSKPLTFP DGCTLPAGIT VVLSIWGLHH NPAVWKNPKV 370 380 390 400 410 420 FDPLRFSQEN SDQRHPYAYL PFSAGSRNCI GQEFAMIELK VTIALILLHF RVTPDPTRPL 430 440 TFPNHFILKP KNGMYLHLKK LSEC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cytochrome P450 Cyp4x1 is a major P450 protein in mouse brain." Al-Anizy M., Horley N.J., Kuo C.W., Gillett L.C., Laughton C.A., Kendall D., Barrett D.A., Parker T., Bell D.R. FEBS J. 273:936-947(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Aorta.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Tissue: Liver, Lung and Trachea.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM040940 mRNA. Translation: CAJ13826.1. AY358537 mRNA. Translation: AAQ88901.1. AK091806 mRNA. Translation: BAC03751.1. AK098065 mRNA. Translation: BAC05226.1. AK131355 mRNA. Translation: BAD18508.1. AL450996 Genomic DNA. Translation: CAH71035.1. AL731892 Genomic DNA. No translation available. CH471059 Genomic DNA. Translation: EAX06882.1. CH471059 Genomic DNA. Translation: EAX06883.1. CH471059 Genomic DNA. Translation: EAX06884.1. BC028102 mRNA. Translation: AAH28102.1.
IPI	IPI00166042.
RefSeq	NP_828847.1. NM_178033.1.
UniGene	Hs.439760.
3D structure databases
ProteinModelPortal	Q8N118.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000360968.
PTM databases
PhosphoSite	Q8N118.
Polymorphism databases
DMDM	48428082.
Proteomic databases
PaxDb	Q8N118.
PRIDE	Q8N118.
Protocols and materials databases
DNASU	260293.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000371901; ENSP00000360968; ENSG00000186377. ENST00000538609; ENSP00000445965; ENSG00000186377.
GeneID	260293.
KEGG	hsa:260293.
UCSC	uc001cqs.3. human.
Organism-specific databases
CTD	260293.
GeneCards	GC01P047489.
HGNC	HGNC:20244. CYP4X1.
HPA	HPA017661.
MIM	614999. gene.
neXtProt	NX_Q8N118.
PharmGKB	PA134933184.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000233833.
HOVERGEN	HBG000182.
InParanoid	Q8N118.
KO	K07428.
OMA	NQPFLMR.
OrthoDB	EOG4V170S.
Gene expression databases
ArrayExpress	Q8N118.
Bgee	Q8N118.
CleanEx	HS_CYP4X1.
Genevestigator	Q8N118.
GermOnline	ENSG00000186377. Homo sapiens.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q8N118.
GenomeRNAi	260293.
NextBio	35516628.
SOURCE	Search...

Entry information

Entry name

CP4X1_HUMAN

Accession

Primary (citable) accession number: Q8N118
Secondary accession number(s): G3V1U1 , Q5VVE5, Q6ZN67, Q8NAZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.