Q8N114 (SHSA5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein shisa-5

Alternative name(s):
Putative NF-kappa-B-activating protein 120
Scotin

Gene names

Name:	SHISA5
Synonyms:	SCOTIN
ORF Names:	PSEC0133

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Can induce apoptosis in a caspase-dependent manner and plays a role in p53/TP53-dependent apoptosis. Ref.1
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein. Nucleus membrane Ref.1.
Induction	Induced in a p53/TP53-dependent manner in response to cellular stress. Ref.1
Domain	The proline-rich region is required for endoplasmic reticulum localization. Ref.1
Sequence similarities	Belongs to the shisa family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Endoplasmic reticulum Membrane Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW induction of apoptosis Inferred from electronic annotation. Source: Compara positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from mutant phenotype Ref.2. Source: UniProtKB
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	signal transducer activity Inferred from mutant phenotype Ref.2. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
WWOX	Q9NZC7	2	EBI-2115556,EBI-4320739

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N114-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N114-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: Missing.

Isoform 3 (identifier: Q8N114-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.
     104-104: S → M

Isoform 4 (identifier: Q8N114-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.
     104-104: S → M
     216-240: GAAAPYPASQPPYNPAYMDAPKAAL → ECPCQL

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 240	212	Protein shisa-5		PRO_0000312878
Regions
	Topological domain	29 – 105	77	Extracellular Potential
	Transmembrane	106 – 126	21	Helical; Potential
	Topological domain	127 – 240	114	Cytoplasmic Potential
	Compositional bias	147 – 153	7	Poly-Thr
	Compositional bias	158 – 230	73	Pro-rich
Natural variations
	Alternative sequence	1 – 103	103	Missing in isoform 3 and isoform 4.		VSP_029953
	Alternative sequence	1 – 31	31	Missing in isoform 2.		VSP_029954
	Alternative sequence	104	1	S → M in isoform 3 and isoform 4.		VSP_029955
	Alternative sequence	216 – 240	25	GAAAP…PKAAL → ECPCQL in isoform 4.		VSP_029956
	Natural variant	216	1	G → R. Corresponds to variant rs35750010 [ dbSNP | Ensembl ].		VAR_054031

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 7C4A8E7A2AD8E272	FASTA	240	25,582
	10 20 30 40 50 60 MTAPVPAPRI LLPLLLLLLL TPPPGARGEV CMASRGLSLF PESCPDFCCG TCDDQYCCSD 70 80 90 100 110 120 VLKKFVWSEE RCAVPEASVP ASVEPVEQLG SALRFRPGYN DPMSGFGATL AVGLTIFVLS 130 140 150 160 170 180 VVTIIICFTC SCCCLYKTCR RPRPVVTTTT STTVVHAPYP QPPSVPPSYP GPSYQGYHTM 190 200 210 220 230 240 PPQPGMPAAP YPMQYPPPYP AQPMGPPAYH ETLAGGAAAP YPASQPPYNP AYMDAPKAAL
	Isoform 2 [UniParc]. Checksum: 523A68BE70B836AF	FASTA	209	22,367
	10 20 30 40 50 60 MASRGLSLFP ESCPDFCCGT CDDQYCCSDV LKKFVWSEER CAVPEASVPA SVEPVEQLGS 70 80 90 100 110 120 ALRFRPGYND PMSGFGATLA VGLTIFVLSV VTIIICFTCS CCCLYKTCRR PRPVVTTTTS 130 140 150 160 170 180 TTVVHAPYPQ PPSVPPSYPG PSYQGYHTMP PQPGMPAAPY PMQYPPPYPA QPMGPPAYHE 190 200 TLAGGAAAPY PASQPPYNPA YMDAPKAAL
	Isoform 3 [UniParc]. Checksum: 7DB8894F341443AD	FASTA	137	14,502
	10 20 30 40 50 60 MGFGATLAVG LTIFVLSVVT IIICFTCSCC CLYKTCRRPR PVVTTTTSTT VVHAPYPQPP 70 80 90 100 110 120 SVPPSYPGPS YQGYHTMPPQ PGMPAAPYPM QYPPPYPAQP MGPPAYHETL AGGAAAPYPA 130 SQPPYNPAYM DAPKAAL
	Isoform 4 [UniParc]. Checksum: 028379F596023F38	FASTA	118	12,661
	10 20 30 40 50 60 MGFGATLAVG LTIFVLSVVT IIICFTCSCC CLYKTCRRPR PVVTTTTSTT VVHAPYPQPP 70 80 90 100 110 SVPPSYPGPS YQGYHTMPPQ PGMPAAPYPM QYPPPYPAQP MGPPAYHETL AGECPCQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Scotin, a novel p53-inducible proapoptotic protein located in the ER and the nuclear membrane." Bourdon J.-C., Renzing J., Robertson P.L., Fernandes K.N., Lane D.P. J. Cell Biol. 158:235-246(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), FUNCTION, INDUCTION, DOMAIN, SUBCELLULAR LOCATION.
[2]	"Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways." Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S. Oncogene 22:3307-3318(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Lung.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Amygdala, Placenta and Teratocarcinoma.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4). Tissue: Brain and Kidney.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Placenta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF520698 mRNA. Translation: AAM74232.1. AH011688 Genomic DNA. Translation: AAM74235.1. AB097007 mRNA. Translation: BAC77360.1. AK056328 mRNA. Translation: BAB71152.1. AK075441 mRNA. Translation: BAC11623.1. AK124652 mRNA. Translation: BAG54061.1. AL117413 mRNA. Translation: CAH10718.1. AL832568 mRNA. Translation: CAH10587.1. CH471055 Genomic DNA. Translation: EAW64885.1. CH471055 Genomic DNA. Translation: EAW64887.1. BC001463 mRNA. Translation: AAH01463.2.
IPI	IPI00166039. IPI00743122. IPI00796691. IPI00876934.
RefSeq	NP_001258994.1. NM_001272065.1. NP_001258995.1. NM_001272066.1. NP_001258996.1. NM_001272067.1. NP_001258997.1. NM_001272068.1. NP_001259011.1. NM_001272082.1. NP_001259012.1. NM_001272083.1. NP_057563.3. NM_016479.4.
UniGene	Hs.414579. Hs.664265.
3D structure databases
ProteinModelPortal	Q8N114.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8N114. 2 interactions.
STRING	9606.ENSP00000296444.
PTM databases
PhosphoSite	Q8N114.
Polymorphism databases
DMDM	74714697.
Proteomic databases
PaxDb	Q8N114.
PRIDE	Q8N114.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000296444; ENSP00000296444; ENSG00000164054. ENST00000426002; ENSP00000390388; ENSG00000164054. ENST00000442747; ENSP00000408223; ENSG00000164054. ENST00000444115; ENSP00000407957; ENSG00000164054.
GeneID	51246.
KEGG	hsa:51246.
UCSC	uc003ctm.2. human. uc003cto.1. human. uc003ctt.3. human.
Organism-specific databases
CTD	51246.
GeneCards	GC03M048485.
HGNC	HGNC:30376. SHISA5.
HPA	HPA042295.
MIM	607290. gene.
neXtProt	NX_Q8N114.
PharmGKB	PA162403295.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG42686.
HOVERGEN	HBG106208.
InParanoid	Q8N114.
KO	K10135.
OMA	MSGFGAT.
OrthoDB	EOG4HX52F.
PhylomeDB	Q8N114.
Gene expression databases
ArrayExpress	Q8N114.
Bgee	Q8N114.
CleanEx	HS_SHISA5.
Genevestigator	Q8N114.
Family and domain databases
InterPro	IPR026910. Shisa. [Graphical view]
Pfam	PF13908. Shisa. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SHISA5. human.
GenomeRNAi	51246.
NextBio	54370.
SOURCE	Search...

Entry information

Entry name

SHSA5_HUMAN

Accession

Primary (citable) accession number: Q8N114
Secondary accession number(s): B3KW99 , Q69YY9, Q7Z433, Q8NHL9, Q96MW8, Q9BV58

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	October 1, 2002
Last modified:	May 29, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.