Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8N114 (SHSA5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein shisa-5
Alternative name(s):
Putative NF-kappa-B-activating protein 120
Scotin
Gene names
Name:SHISA5
Synonyms:SCOTIN
ORF Names:PSEC0133
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length240 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Can induce apoptosis in a caspase-dependent manner and plays a role in p53/TP53-dependent apoptosis. Ref.1

Subunit structure

Interacts with PDCD6; PDCD6 can stabilze SHISA5. Ref.7

Subcellular location

Endoplasmic reticulum membrane; Single-pass type I membrane protein. Nucleus membrane Ref.1.

Induction

Induced in a p53/TP53-dependent manner in response to cellular stress. Ref.1

Domain

The proline-rich region is required for endoplasmic reticulum localization. Ref.1

Sequence similarities

Belongs to the shisa family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Pdcd6P128155EBI-2115556,EBI-309164From a different organism.
WWOXQ9NZC72EBI-2115556,EBI-4320739

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8N114-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8N114-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: Missing.
Isoform 3 (identifier: Q8N114-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.
     104-104: S → M
Isoform 4 (identifier: Q8N114-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.
     104-104: S → M
     216-240: GAAAPYPASQPPYNPAYMDAPKAAL → ECPCQL

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 240212Protein shisa-5
PRO_0000312878

Regions

Topological domain29 – 10577Extracellular Potential
Transmembrane106 – 12621Helical; Potential
Topological domain127 – 240114Cytoplasmic Potential
Compositional bias147 – 1537Poly-Thr
Compositional bias158 – 23073Pro-rich

Natural variations

Alternative sequence1 – 103103Missing in isoform 3 and isoform 4.
VSP_029953
Alternative sequence1 – 3131Missing in isoform 2.
VSP_029954
Alternative sequence1041S → M in isoform 3 and isoform 4.
VSP_029955
Alternative sequence216 – 24025GAAAP…PKAAL → ECPCQL in isoform 4.
VSP_029956
Natural variant2161G → R.
Corresponds to variant rs35750010 [ dbSNP | Ensembl ].
VAR_054031

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 7C4A8E7A2AD8E272

FASTA24025,582
        10         20         30         40         50         60 
MTAPVPAPRI LLPLLLLLLL TPPPGARGEV CMASRGLSLF PESCPDFCCG TCDDQYCCSD 

        70         80         90        100        110        120 
VLKKFVWSEE RCAVPEASVP ASVEPVEQLG SALRFRPGYN DPMSGFGATL AVGLTIFVLS 

       130        140        150        160        170        180 
VVTIIICFTC SCCCLYKTCR RPRPVVTTTT STTVVHAPYP QPPSVPPSYP GPSYQGYHTM 

       190        200        210        220        230        240 
PPQPGMPAAP YPMQYPPPYP AQPMGPPAYH ETLAGGAAAP YPASQPPYNP AYMDAPKAAL 

« Hide

Isoform 2 [UniParc].

Checksum: 523A68BE70B836AF
Show »

FASTA20922,367
Isoform 3 [UniParc].

Checksum: 7DB8894F341443AD
Show »

FASTA13714,502
Isoform 4 [UniParc].

Checksum: 028379F596023F38
Show »

FASTA11812,661

References

« Hide 'large scale' references
[1]"Scotin, a novel p53-inducible proapoptotic protein located in the ER and the nuclear membrane."
Bourdon J.-C., Renzing J., Robertson P.L., Fernandes K.N., Lane D.P.
J. Cell Biol. 158:235-246(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), FUNCTION, INDUCTION, DOMAIN, SUBCELLULAR LOCATION.
[2]"Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways."
Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S.
Oncogene 22:3307-3318(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Lung.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Amygdala, Placenta and Teratocarcinoma.
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4).
Tissue: Brain and Kidney.
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Placenta.
[7]"The calcium binding protein ALG-2 binds and stabilizes Scotin, a p53-inducible gene product localized at the endoplasmic reticulum membrane."
Draeby I., Woods Y.L., la Cour J.M., Mollerup J., Bourdon J.C., Berchtold M.W.
Arch. Biochem. Biophys. 467:87-94(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH WITH PDCD6.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF520698 mRNA. Translation: AAM74232.1.
AH011688 Genomic DNA. Translation: AAM74235.1.
AB097007 mRNA. Translation: BAC77360.1.
AK056328 mRNA. Translation: BAB71152.1.
AK075441 mRNA. Translation: BAC11623.1.
AK124652 mRNA. Translation: BAG54061.1.
AL117413 mRNA. Translation: CAH10718.1.
AL832568 mRNA. Translation: CAH10587.1.
CH471055 Genomic DNA. Translation: EAW64885.1.
CH471055 Genomic DNA. Translation: EAW64887.1.
BC001463 mRNA. Translation: AAH01463.2.
CCDSCCDS2770.1. [Q8N114-1]
CCDS63621.1. [Q8N114-3]
CCDS63622.1. [Q8N114-2]
RefSeqNP_001258994.1. NM_001272065.1.
NP_001258995.1. NM_001272066.1. [Q8N114-2]
NP_001258996.1. NM_001272067.1. [Q8N114-2]
NP_001258997.1. NM_001272068.1. [Q8N114-2]
NP_001259011.1. NM_001272082.1. [Q8N114-3]
NP_001259012.1. NM_001272083.1. [Q8N114-4]
NP_057563.3. NM_016479.4. [Q8N114-1]
XP_005265262.1. XM_005265205.1. [Q8N114-2]
XP_006713251.1. XM_006713188.1. [Q8N114-2]
UniGeneHs.414579.
Hs.664265.

3D structure databases

ProteinModelPortalQ8N114.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119403. 3 interactions.
IntActQ8N114. 3 interactions.
STRING9606.ENSP00000296444.

PTM databases

PhosphoSiteQ8N114.

Polymorphism databases

DMDM74714697.

Proteomic databases

PaxDbQ8N114.
PRIDEQ8N114.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000296444; ENSP00000296444; ENSG00000164054. [Q8N114-1]
ENST00000426002; ENSP00000390388; ENSG00000164054. [Q8N114-3]
ENST00000442747; ENSP00000408223; ENSG00000164054. [Q8N114-2]
ENST00000444115; ENSP00000407957; ENSG00000164054. [Q8N114-2]
GeneID51246.
KEGGhsa:51246.
UCSCuc003ctm.3. human. [Q8N114-3]
uc003cto.1. human. [Q8N114-1]
uc003ctt.4. human. [Q8N114-4]

Organism-specific databases

CTD51246.
GeneCardsGC03M048485.
HGNCHGNC:30376. SHISA5.
HPAHPA042295.
MIM607290. gene.
neXtProtNX_Q8N114.
PharmGKBPA162403295.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG42686.
HOVERGENHBG106208.
InParanoidQ8N114.
KOK10135.
OMAMDPPKAA.
OrthoDBEOG7ZKSD0.
PhylomeDBQ8N114.
TreeFamTF332572.

Gene expression databases

ArrayExpressQ8N114.
BgeeQ8N114.
CleanExHS_SHISA5.
GenevestigatorQ8N114.

Family and domain databases

InterProIPR026910. Shisa.
[Graphical view]
PfamPF13908. Shisa. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSHISA5. human.
GenomeRNAi51246.
NextBio54370.
PROQ8N114.
SOURCESearch...

Entry information

Entry nameSHSA5_HUMAN
AccessionPrimary (citable) accession number: Q8N114
Secondary accession number(s): B3KW99 expand/collapse secondary AC list , Q69YY9, Q7Z433, Q8NHL9, Q96MW8, Q9BV58
Entry history
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM