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Q8N114

- SHSA5_HUMAN

UniProt

Q8N114 - SHSA5_HUMAN

Protein

Protein shisa-5

Gene

SHISA5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Can induce apoptosis in a caspase-dependent manner and plays a role in p53/TP53-dependent apoptosis.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. signal transducer activity Source: UniProtKB
    3. WW domain binding Source: UniProtKB

    GO - Biological processi

    1. intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: Ensembl
    2. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB

    Keywords - Biological processi

    Apoptosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein shisa-5
    Alternative name(s):
    Putative NF-kappa-B-activating protein 120
    Scotin
    Gene namesi
    Name:SHISA5
    Synonyms:SCOTIN
    ORF Names:PSEC0133
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:30376. SHISA5.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Single-pass type I membrane protein 1 Publication. Nucleus membrane 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. extracellular vesicular exosome Source: UniProt
    3. integral component of membrane Source: UniProtKB-KW
    4. nuclear membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162403295.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Chaini29 – 240212Protein shisa-5PRO_0000312878Add
    BLAST

    Proteomic databases

    PaxDbiQ8N114.
    PRIDEiQ8N114.

    PTM databases

    PhosphoSiteiQ8N114.

    Expressioni

    Inductioni

    Induced in a p53/TP53-dependent manner in response to cellular stress.1 Publication

    Gene expression databases

    ArrayExpressiQ8N114.
    BgeeiQ8N114.
    CleanExiHS_SHISA5.
    GenevestigatoriQ8N114.

    Organism-specific databases

    HPAiHPA042295.

    Interactioni

    Subunit structurei

    Interacts with PDCD6; PDCD6 can stabilze SHISA5.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Pdcd6P128155EBI-2115556,EBI-309164From a different organism.
    WWOXQ9NZC72EBI-2115556,EBI-4320739

    Protein-protein interaction databases

    BioGridi119403. 3 interactions.
    IntActiQ8N114. 3 interactions.
    STRINGi9606.ENSP00000296444.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8N114.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini29 – 10577ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini127 – 240114CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei106 – 12621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi147 – 1537Poly-Thr
    Compositional biasi158 – 23073Pro-richAdd
    BLAST

    Domaini

    The proline-rich region is required for endoplasmic reticulum localization.1 Publication

    Sequence similaritiesi

    Belongs to the shisa family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG42686.
    HOVERGENiHBG106208.
    InParanoidiQ8N114.
    KOiK10135.
    OMAiMDPPKAA.
    OrthoDBiEOG7ZKSD0.
    PhylomeDBiQ8N114.
    TreeFamiTF332572.

    Family and domain databases

    InterProiIPR026910. Shisa.
    [Graphical view]
    PfamiPF13908. Shisa. 1 hit.
    [Graphical view]

    Sequences (5)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 5 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8N114-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTAPVPAPRI LLPLLLLLLL TPPPGARGEV CMASRGLSLF PESCPDFCCG    50
    TCDDQYCCSD VLKKFVWSEE RCAVPEASVP ASVEPVEQLG SALRFRPGYN 100
    DPMSGFGATL AVGLTIFVLS VVTIIICFTC SCCCLYKTCR RPRPVVTTTT 150
    STTVVHAPYP QPPSVPPSYP GPSYQGYHTM PPQPGMPAAP YPMQYPPPYP 200
    AQPMGPPAYH ETLAGGAAAP YPASQPPYNP AYMDAPKAAL 240
    Length:240
    Mass (Da):25,582
    Last modified:October 1, 2002 - v1
    Checksum:i7C4A8E7A2AD8E272
    GO
    Isoform 2 (identifier: Q8N114-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-31: Missing.

    Show »
    Length:209
    Mass (Da):22,367
    Checksum:i523A68BE70B836AF
    GO
    Isoform 3 (identifier: Q8N114-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-103: Missing.
         104-104: S → M

    Show »
    Length:137
    Mass (Da):14,502
    Checksum:i7DB8894F341443AD
    GO
    Isoform 4 (identifier: Q8N114-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-103: Missing.
         104-104: S → M
         216-240: GAAAPYPASQPPYNPAYMDAPKAAL → ECPCQL

    Show »
    Length:118
    Mass (Da):12,661
    Checksum:i028379F596023F38
    GO
    Isoform 5 (identifier: Q8N114-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         71-77: Missing.

    Note: Gene prediction based on EST data.

    Show »
    Length:233
    Mass (Da):24,855
    Checksum:i8302434F0F5AF946
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti216 – 2161G → R.
    Corresponds to variant rs35750010 [ dbSNP | Ensembl ].
    VAR_054031

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 103103Missing in isoform 3 and isoform 4. 3 PublicationsVSP_029953Add
    BLAST
    Alternative sequencei1 – 3131Missing in isoform 2. 1 PublicationVSP_029954Add
    BLAST
    Alternative sequencei71 – 777Missing in isoform 5. CuratedVSP_055712
    Alternative sequencei104 – 1041S → M in isoform 3 and isoform 4. 3 PublicationsVSP_029955
    Alternative sequencei216 – 24025GAAAP…PKAAL → ECPCQL in isoform 4. 1 PublicationVSP_029956Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF520698 mRNA. Translation: AAM74232.1.
    AH011688 Genomic DNA. Translation: AAM74235.1.
    AB097007 mRNA. Translation: BAC77360.1.
    AK056328 mRNA. Translation: BAB71152.1.
    AK075441 mRNA. Translation: BAC11623.1.
    AK124652 mRNA. Translation: BAG54061.1.
    AL117413 mRNA. Translation: CAH10718.1.
    AL832568 mRNA. Translation: CAH10587.1.
    AC104448 Genomic DNA. No translation available.
    AC134772 Genomic DNA. No translation available.
    CH471055 Genomic DNA. Translation: EAW64885.1.
    CH471055 Genomic DNA. Translation: EAW64887.1.
    BC001463 mRNA. Translation: AAH01463.2.
    CCDSiCCDS2770.1. [Q8N114-1]
    CCDS63621.1. [Q8N114-3]
    CCDS63622.1. [Q8N114-2]
    RefSeqiNP_001258994.1. NM_001272065.1.
    NP_001258995.1. NM_001272066.1. [Q8N114-2]
    NP_001258996.1. NM_001272067.1. [Q8N114-2]
    NP_001258997.1. NM_001272068.1. [Q8N114-2]
    NP_001259011.1. NM_001272082.1. [Q8N114-3]
    NP_001259012.1. NM_001272083.1. [Q8N114-4]
    NP_057563.3. NM_016479.4. [Q8N114-1]
    XP_005265262.1. XM_005265205.1. [Q8N114-2]
    XP_006713251.1. XM_006713188.1. [Q8N114-2]
    UniGeneiHs.414579.
    Hs.664265.

    Genome annotation databases

    EnsembliENST00000296444; ENSP00000296444; ENSG00000164054. [Q8N114-1]
    ENST00000426002; ENSP00000390388; ENSG00000164054. [Q8N114-3]
    ENST00000442747; ENSP00000408223; ENSG00000164054. [Q8N114-2]
    ENST00000443308; ENSP00000395373; ENSG00000164054. [Q8N114-5]
    ENST00000444115; ENSP00000407957; ENSG00000164054. [Q8N114-2]
    GeneIDi51246.
    KEGGihsa:51246.
    UCSCiuc003ctm.3. human. [Q8N114-3]
    uc003cto.1. human. [Q8N114-1]
    uc003ctt.4. human. [Q8N114-4]

    Polymorphism databases

    DMDMi74714697.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF520698 mRNA. Translation: AAM74232.1 .
    AH011688 Genomic DNA. Translation: AAM74235.1 .
    AB097007 mRNA. Translation: BAC77360.1 .
    AK056328 mRNA. Translation: BAB71152.1 .
    AK075441 mRNA. Translation: BAC11623.1 .
    AK124652 mRNA. Translation: BAG54061.1 .
    AL117413 mRNA. Translation: CAH10718.1 .
    AL832568 mRNA. Translation: CAH10587.1 .
    AC104448 Genomic DNA. No translation available.
    AC134772 Genomic DNA. No translation available.
    CH471055 Genomic DNA. Translation: EAW64885.1 .
    CH471055 Genomic DNA. Translation: EAW64887.1 .
    BC001463 mRNA. Translation: AAH01463.2 .
    CCDSi CCDS2770.1. [Q8N114-1 ]
    CCDS63621.1. [Q8N114-3 ]
    CCDS63622.1. [Q8N114-2 ]
    RefSeqi NP_001258994.1. NM_001272065.1.
    NP_001258995.1. NM_001272066.1. [Q8N114-2 ]
    NP_001258996.1. NM_001272067.1. [Q8N114-2 ]
    NP_001258997.1. NM_001272068.1. [Q8N114-2 ]
    NP_001259011.1. NM_001272082.1. [Q8N114-3 ]
    NP_001259012.1. NM_001272083.1. [Q8N114-4 ]
    NP_057563.3. NM_016479.4. [Q8N114-1 ]
    XP_005265262.1. XM_005265205.1. [Q8N114-2 ]
    XP_006713251.1. XM_006713188.1. [Q8N114-2 ]
    UniGenei Hs.414579.
    Hs.664265.

    3D structure databases

    ProteinModelPortali Q8N114.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119403. 3 interactions.
    IntActi Q8N114. 3 interactions.
    STRINGi 9606.ENSP00000296444.

    PTM databases

    PhosphoSitei Q8N114.

    Polymorphism databases

    DMDMi 74714697.

    Proteomic databases

    PaxDbi Q8N114.
    PRIDEi Q8N114.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000296444 ; ENSP00000296444 ; ENSG00000164054 . [Q8N114-1 ]
    ENST00000426002 ; ENSP00000390388 ; ENSG00000164054 . [Q8N114-3 ]
    ENST00000442747 ; ENSP00000408223 ; ENSG00000164054 . [Q8N114-2 ]
    ENST00000443308 ; ENSP00000395373 ; ENSG00000164054 . [Q8N114-5 ]
    ENST00000444115 ; ENSP00000407957 ; ENSG00000164054 . [Q8N114-2 ]
    GeneIDi 51246.
    KEGGi hsa:51246.
    UCSCi uc003ctm.3. human. [Q8N114-3 ]
    uc003cto.1. human. [Q8N114-1 ]
    uc003ctt.4. human. [Q8N114-4 ]

    Organism-specific databases

    CTDi 51246.
    GeneCardsi GC03M048485.
    HGNCi HGNC:30376. SHISA5.
    HPAi HPA042295.
    MIMi 607290. gene.
    neXtProti NX_Q8N114.
    PharmGKBi PA162403295.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG42686.
    HOVERGENi HBG106208.
    InParanoidi Q8N114.
    KOi K10135.
    OMAi MDPPKAA.
    OrthoDBi EOG7ZKSD0.
    PhylomeDBi Q8N114.
    TreeFami TF332572.

    Miscellaneous databases

    ChiTaRSi SHISA5. human.
    GenomeRNAii 51246.
    NextBioi 54370.
    PROi Q8N114.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8N114.
    Bgeei Q8N114.
    CleanExi HS_SHISA5.
    Genevestigatori Q8N114.

    Family and domain databases

    InterProi IPR026910. Shisa.
    [Graphical view ]
    Pfami PF13908. Shisa. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Scotin, a novel p53-inducible proapoptotic protein located in the ER and the nuclear membrane."
      Bourdon J.-C., Renzing J., Robertson P.L., Fernandes K.N., Lane D.P.
      J. Cell Biol. 158:235-246(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), FUNCTION, INDUCTION, DOMAIN, SUBCELLULAR LOCATION.
    2. "Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways."
      Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S.
      Oncogene 22:3307-3318(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Tissue: Lung.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Amygdala, Placenta and Teratocarcinoma.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4).
      Tissue: Brain and Kidney.
    5. "The DNA sequence and comparative analysis of human chromosome 5."
      Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
      , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
      Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Tissue: Placenta.
    8. "The calcium binding protein ALG-2 binds and stabilizes Scotin, a p53-inducible gene product localized at the endoplasmic reticulum membrane."
      Draeby I., Woods Y.L., la Cour J.M., Mollerup J., Bourdon J.C., Berchtold M.W.
      Arch. Biochem. Biophys. 467:87-94(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH WITH PDCD6.

    Entry informationi

    Entry nameiSHSA5_HUMAN
    AccessioniPrimary (citable) accession number: Q8N114
    Secondary accession number(s): B3KW99
    , F8W9N8, Q69YY9, Q7Z433, Q8NHL9, Q96MW8, Q9BV58
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 4, 2007
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3