Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

T-cell activation Rho GTPase-activating protein

Gene

TAGAP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May function as a GTPase-activating protein and may play important roles during T-cell activation.1 Publication

GO - Molecular functioni

  1. guanyl-nucleotide exchange factor activity Source: UniProtKB-KW

GO - Biological processi

  1. regulation of small GTPase mediated signal transduction Source: Reactome
  2. small GTPase mediated signal transduction Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiREACT_11051. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell activation Rho GTPase-activating protein
Alternative name(s):
T-cell activation GTPase-activating protein
Gene namesi
Name:TAGAP
Synonyms:TAGAP1
ORF Names:FKSG15
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:15669. TAGAP.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38016.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 731731T-cell activation Rho GTPase-activating proteinPRO_0000056719Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei400 – 4001Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8N103.
PRIDEiQ8N103.

PTM databases

PhosphoSiteiQ8N103.

Expressioni

Gene expression databases

BgeeiQ8N103.
CleanExiHS_TAGAP.
GenevestigatoriQ8N103.

Organism-specific databases

HPAiHPA031000.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000356033.

Structurei

3D structure databases

ProteinModelPortaliQ8N103.
SMRiQ8N103. Positions 83-277.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini88 – 277190Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG282252.
GeneTreeiENSGT00530000063299.
HOGENOMiHOG000139745.
HOVERGENiHBG071607.
InParanoidiQ8N103.
OMAiKHSMSFS.
OrthoDBiEOG7NCV2Q.
PhylomeDBiQ8N103.
TreeFamiTF331062.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N103-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLRSSHNAS KTLNANNMET LIECQSEGDI KEHPLLASCE SEDSICQLIE
60 70 80 90 100
VKKRKKVLSW PFLMRRLSPA SDFSGALETD LKASLFDQPL SIICGDSDTL
110 120 130 140 150
PRPIQDILTI LCLKGPSTEG IFRRAANEKA RKELKEELNS GDAVDLERLP
160 170 180 190 200
VHLLAVVFKD FLRSIPRKLL SSDLFEEWMG ALEMQDEEDR IEALKQVADK
210 220 230 240 250
LPRPNLLLLK HLVYVLHLIS KNSEVNRMDS SNLAICIGPN MLTLENDQSL
260 270 280 290 300
SFEAQKDLNN KVKTLVEFLI DNCFEIFGEN IPVHSSITSD DSLEHTDSSD
310 320 330 340 350
VSTLQNDSAY DSNDPDVESN SSSGISSPSR QPQVPMATAA GLDSAGPQDA
360 370 380 390 400
REVSPEPIVS TVARLKSSLA QPDRRYSEPS MPSSQECLES RVTNQTLTKS
410 420 430 440 450
EGDFPVPRVG SRLESEEAED PFPEEVFPAV QGKTKRPVDL KIKNLAPGSV
460 470 480 490 500
LPRALVLKAF SSSSLDASSD SSPVASPSSP KRNFFSRHQS FTTKTEKGKP
510 520 530 540 550
SREIKKHSMS FTFAPHKKVL TKNLSAGSGK SQDFTRDHVP RGVRKESQLA
560 570 580 590 600
GRIVQENGCE THNQTARGFC LRPHALSVDD VFQGADWERP GSPPSYEEAM
610 620 630 640 650
QGPAARLVAS ESQTVGSMTV GSMRARMLEA HCLLPPLPPA HHVEDSRHRG
660 670 680 690 700
SKEPLPGHGL SPLPERWKQS RTVHASGDSL GHVSGPGRPE LLPLRTVSES
710 720 730
VQRNKRDCLV RRCSQPVFEA DQFQYAKESY I
Length:731
Mass (Da):80,703
Last modified:September 30, 2002 - v1
Checksum:i9D8768258A0A681E
GO
Isoform 2 (identifier: Q8N103-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-178: Missing.

Show »
Length:553
Mass (Da):60,669
Checksum:i9728446CFC104C5D
GO
Isoform 3 (identifier: Q8N103-3) [UniParc]FASTAAdd to basket

Also known as: FKSG15

The sequence of this isoform differs from the canonical sequence as follows:
     1-209: Missing.
     210-261: KHLVYVLHLI...FEAQKDLNNK → MAATAAAVVA...LALEEQEKVE

Note: No experimental confirmation available. Dubious isoform. The N-terminus appears to be derived from exons of the FGFR1OP locus which is located on the opposing strand of chromosome 6 at a distance of several Mb.

Show »
Length:522
Mass (Da):56,919
Checksum:i813D0EF6C7956A16
GO
Isoform 4 (identifier: Q8N103-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     263-266: KTLV → CSAY
     267-731: Missing.

Note: No experimental confirmation available.

Show »
Length:266
Mass (Da):29,977
Checksum:i93A7C4A94871F9A0
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti346 – 3461G → D.
Corresponds to variant rs35263580 [ dbSNP | Ensembl ].
VAR_049146

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 209209Missing in isoform 3. 1 PublicationVSP_015868Add
BLAST
Alternative sequencei1 – 178178Missing in isoform 2. 1 PublicationVSP_015869Add
BLAST
Alternative sequencei210 – 26152KHLVY…DLNNK → MAATAAAVVAEEDTELRDLL VQTLENSGVLNRIKAELRAA VFLALEEQEKVE in isoform 3. 1 PublicationVSP_015870Add
BLAST
Alternative sequencei263 – 2664KTLV → CSAY in isoform 4. 1 PublicationVSP_015871
Alternative sequencei267 – 731465Missing in isoform 4. 1 PublicationVSP_015872Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF385429 mRNA. Translation: AAM43830.1.
AF385430 mRNA. Translation: AAM43831.1.
AF314817 mRNA. Translation: AAL16675.1.
AK097090 mRNA. Translation: BAC04947.1.
AL035530 Genomic DNA. Translation: CAI19231.1.
AL035530 Genomic DNA. Translation: CAI19232.1.
AL035530 Genomic DNA. Translation: CAI19233.1.
BC015859 mRNA. Translation: AAH15859.1.
BC111731 mRNA. Translation: AAI11732.1.
CCDSiCCDS5261.1. [Q8N103-1]
CCDS5262.1. [Q8N103-2]
CCDS5263.1. [Q8N103-4]
RefSeqiNP_001265662.1. NM_001278733.1.
NP_473455.2. NM_054114.4. [Q8N103-1]
NP_620165.1. NM_138810.3. [Q8N103-4]
NP_687034.1. NM_152133.2. [Q8N103-2]
UniGeneiHs.529984.

Genome annotation databases

EnsembliENST00000326965; ENSP00000322650; ENSG00000164691. [Q8N103-2]
ENST00000338313; ENSP00000340217; ENSG00000164691. [Q8N103-4]
ENST00000367066; ENSP00000356033; ENSG00000164691. [Q8N103-1]
GeneIDi117289.
KEGGihsa:117289.
UCSCiuc003qrz.3. human. [Q8N103-1]
uc003qsb.3. human. [Q8N103-4]

Polymorphism databases

DMDMi74750874.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF385429 mRNA. Translation: AAM43830.1.
AF385430 mRNA. Translation: AAM43831.1.
AF314817 mRNA. Translation: AAL16675.1.
AK097090 mRNA. Translation: BAC04947.1.
AL035530 Genomic DNA. Translation: CAI19231.1.
AL035530 Genomic DNA. Translation: CAI19232.1.
AL035530 Genomic DNA. Translation: CAI19233.1.
BC015859 mRNA. Translation: AAH15859.1.
BC111731 mRNA. Translation: AAI11732.1.
CCDSiCCDS5261.1. [Q8N103-1]
CCDS5262.1. [Q8N103-2]
CCDS5263.1. [Q8N103-4]
RefSeqiNP_001265662.1. NM_001278733.1.
NP_473455.2. NM_054114.4. [Q8N103-1]
NP_620165.1. NM_138810.3. [Q8N103-4]
NP_687034.1. NM_152133.2. [Q8N103-2]
UniGeneiHs.529984.

3D structure databases

ProteinModelPortaliQ8N103.
SMRiQ8N103. Positions 83-277.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000356033.

PTM databases

PhosphoSiteiQ8N103.

Polymorphism databases

DMDMi74750874.

Proteomic databases

PaxDbiQ8N103.
PRIDEiQ8N103.

Protocols and materials databases

DNASUi117289.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000326965; ENSP00000322650; ENSG00000164691. [Q8N103-2]
ENST00000338313; ENSP00000340217; ENSG00000164691. [Q8N103-4]
ENST00000367066; ENSP00000356033; ENSG00000164691. [Q8N103-1]
GeneIDi117289.
KEGGihsa:117289.
UCSCiuc003qrz.3. human. [Q8N103-1]
uc003qsb.3. human. [Q8N103-4]

Organism-specific databases

CTDi117289.
GeneCardsiGC06M159457.
H-InvDBHIX0006337.
HGNCiHGNC:15669. TAGAP.
HPAiHPA031000.
MIMi609667. gene.
neXtProtiNX_Q8N103.
PharmGKBiPA38016.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG282252.
GeneTreeiENSGT00530000063299.
HOGENOMiHOG000139745.
HOVERGENiHBG071607.
InParanoidiQ8N103.
OMAiKHSMSFS.
OrthoDBiEOG7NCV2Q.
PhylomeDBiQ8N103.
TreeFamiTF331062.

Enzyme and pathway databases

ReactomeiREACT_11051. Rho GTPase cycle.

Miscellaneous databases

ChiTaRSiTAGAP. human.
GenomeRNAii117289.
NextBioi80198.
PROiQ8N103.
SOURCEiSearch...

Gene expression databases

BgeeiQ8N103.
CleanExiHS_TAGAP.
GenevestigatoriQ8N103.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "T lymphocyte activation gene identification by coregulated expression on DNA microarrays."
    Mao M., Biery M.C., Kobayashi S.V., Ward T., Schimmack G., Burchard J., Schelter J.M., Dai H., He Y.D., Linsley P.S.
    Genomics 83:989-999(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION.
  2. "FKSG15, a novel 57 kDa protein, shares the N-terminal region with FOP."
    Wang Y.-G., Gong L.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Spleen.
  4. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
    Tissue: Lung.
  6. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-400, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiTAGAP_HUMAN
AccessioniPrimary (citable) accession number: Q8N103
Secondary accession number(s): Q2NKM8
, Q8NI40, Q96KZ2, Q96QA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2005
Last sequence update: September 30, 2002
Last modified: March 3, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.