Q8N103 (TAGAP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
T-cell activation Rho GTPase-activating protein

Alternative name(s):
T-cell activation GTPase-activating protein

Gene names

Name:	TAGAP
Synonyms:	TAGAP1
ORF Names:	FKSG15

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	731 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May function as a GTPase-activating protein and may play important roles during T-cell activation. Ref.1
Sequence similarities	Contains 1 Rho-GAP domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Guanine-nucleotide releasing factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of small GTPase mediated signal transduction Traceable author statement. Source: Reactome small GTPase mediated signal transduction Traceable author statement. Source: Reactome
Cellular component	cytosol Traceable author statement. Source: Reactome
Molecular function	guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N103-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N103-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-178: Missing.

Isoform 3 (identifier: Q8N103-3)

Also known as: FKSG15;

The sequence of this isoform differs from the canonical sequence as follows:
     1-209: Missing.
     210-261: KHLVYVLHLI...FEAQKDLNNK → MAATAAAVVA...LALEEQEKVE

Note: No experimental confirmation available. Dubious isoform. The N-terminus appears to be derived from exons of the FGFR1OP locus which is located on the opposing strand of chromosome 6 at a distance of several Mb.

Isoform 4 (identifier: Q8N103-4)

The sequence of this isoform differs from the canonical sequence as follows:
     263-266: KTLV → CSAY
     267-731: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 731	731	T-cell activation Rho GTPase-activating protein		PRO_0000056719
Regions
	Domain	88 – 277	190	Rho-GAP
Natural variations
	Alternative sequence	1 – 209	209	Missing in isoform 3.		VSP_015868
	Alternative sequence	1 – 178	178	Missing in isoform 2.		VSP_015869
	Alternative sequence	210 – 261	52	KHLVY…DLNNK → MAATAAAVVAEEDTELRDLL VQTLENSGVLNRIKAELRAA VFLALEEQEKVE in isoform 3.		VSP_015870
	Alternative sequence	263 – 266	4	KTLV → CSAY in isoform 4.		VSP_015871
	Alternative sequence	267 – 731	465	Missing in isoform 4.		VSP_015872
	Natural variant	346	1	G → D. Corresponds to variant rs35263580 [ dbSNP | Ensembl ].		VAR_049146

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 9D8768258A0A681E	FASTA	731	80,703
	10 20 30 40 50 60 MKLRSSHNAS KTLNANNMET LIECQSEGDI KEHPLLASCE SEDSICQLIE VKKRKKVLSW 70 80 90 100 110 120 PFLMRRLSPA SDFSGALETD LKASLFDQPL SIICGDSDTL PRPIQDILTI LCLKGPSTEG 130 140 150 160 170 180 IFRRAANEKA RKELKEELNS GDAVDLERLP VHLLAVVFKD FLRSIPRKLL SSDLFEEWMG 190 200 210 220 230 240 ALEMQDEEDR IEALKQVADK LPRPNLLLLK HLVYVLHLIS KNSEVNRMDS SNLAICIGPN 250 260 270 280 290 300 MLTLENDQSL SFEAQKDLNN KVKTLVEFLI DNCFEIFGEN IPVHSSITSD DSLEHTDSSD 310 320 330 340 350 360 VSTLQNDSAY DSNDPDVESN SSSGISSPSR QPQVPMATAA GLDSAGPQDA REVSPEPIVS 370 380 390 400 410 420 TVARLKSSLA QPDRRYSEPS MPSSQECLES RVTNQTLTKS EGDFPVPRVG SRLESEEAED 430 440 450 460 470 480 PFPEEVFPAV QGKTKRPVDL KIKNLAPGSV LPRALVLKAF SSSSLDASSD SSPVASPSSP 490 500 510 520 530 540 KRNFFSRHQS FTTKTEKGKP SREIKKHSMS FTFAPHKKVL TKNLSAGSGK SQDFTRDHVP 550 560 570 580 590 600 RGVRKESQLA GRIVQENGCE THNQTARGFC LRPHALSVDD VFQGADWERP GSPPSYEEAM 610 620 630 640 650 660 QGPAARLVAS ESQTVGSMTV GSMRARMLEA HCLLPPLPPA HHVEDSRHRG SKEPLPGHGL 670 680 690 700 710 720 SPLPERWKQS RTVHASGDSL GHVSGPGRPE LLPLRTVSES VQRNKRDCLV RRCSQPVFEA 730 DQFQYAKESY I
	Isoform 2 [UniParc]. Checksum: 9728446CFC104C5D	FASTA	553	60,669
	10 20 30 40 50 60 MGALEMQDEE DRIEALKQVA DKLPRPNLLL LKHLVYVLHL ISKNSEVNRM DSSNLAICIG 70 80 90 100 110 120 PNMLTLENDQ SLSFEAQKDL NNKVKTLVEF LIDNCFEIFG ENIPVHSSIT SDDSLEHTDS 130 140 150 160 170 180 SDVSTLQNDS AYDSNDPDVE SNSSSGISSP SRQPQVPMAT AAGLDSAGPQ DAREVSPEPI 190 200 210 220 230 240 VSTVARLKSS LAQPDRRYSE PSMPSSQECL ESRVTNQTLT KSEGDFPVPR VGSRLESEEA 250 260 270 280 290 300 EDPFPEEVFP AVQGKTKRPV DLKIKNLAPG SVLPRALVLK AFSSSSLDAS SDSSPVASPS 310 320 330 340 350 360 SPKRNFFSRH QSFTTKTEKG KPSREIKKHS MSFTFAPHKK VLTKNLSAGS GKSQDFTRDH 370 380 390 400 410 420 VPRGVRKESQ LAGRIVQENG CETHNQTARG FCLRPHALSV DDVFQGADWE RPGSPPSYEE 430 440 450 460 470 480 AMQGPAARLV ASESQTVGSM TVGSMRARML EAHCLLPPLP PAHHVEDSRH RGSKEPLPGH 490 500 510 520 530 540 GLSPLPERWK QSRTVHASGD SLGHVSGPGR PELLPLRTVS ESVQRNKRDC LVRRCSQPVF 550 EADQFQYAKE SYI
	Isoform 3 (FKSG15) [UniParc]. Checksum: 813D0EF6C7956A16	FASTA	522	56,919
	10 20 30 40 50 60 MAATAAAVVA EEDTELRDLL VQTLENSGVL NRIKAELRAA VFLALEEQEK VEVKTLVEFL 70 80 90 100 110 120 IDNCFEIFGE NIPVHSSITS DDSLEHTDSS DVSTLQNDSA YDSNDPDVES NSSSGISSPS 130 140 150 160 170 180 RQPQVPMATA AGLDSAGPQD AREVSPEPIV STVARLKSSL AQPDRRYSEP SMPSSQECLE 190 200 210 220 230 240 SRVTNQTLTK SEGDFPVPRV GSRLESEEAE DPFPEEVFPA VQGKTKRPVD LKIKNLAPGS 250 260 270 280 290 300 VLPRALVLKA FSSSSLDASS DSSPVASPSS PKRNFFSRHQ SFTTKTEKGK PSREIKKHSM 310 320 330 340 350 360 SFTFAPHKKV LTKNLSAGSG KSQDFTRDHV PRGVRKESQL AGRIVQENGC ETHNQTARGF 370 380 390 400 410 420 CLRPHALSVD DVFQGADWER PGSPPSYEEA MQGPAARLVA SESQTVGSMT VGSMRARMLE 430 440 450 460 470 480 AHCLLPPLPP AHHVEDSRHR GSKEPLPGHG LSPLPERWKQ SRTVHASGDS LGHVSGPGRP 490 500 510 520 ELLPLRTVSE SVQRNKRDCL VRRCSQPVFE ADQFQYAKES YI
	Isoform 4 [UniParc]. Checksum: 93A7C4A94871F9A0	FASTA	266	29,977
	10 20 30 40 50 60 MKLRSSHNAS KTLNANNMET LIECQSEGDI KEHPLLASCE SEDSICQLIE VKKRKKVLSW 70 80 90 100 110 120 PFLMRRLSPA SDFSGALETD LKASLFDQPL SIICGDSDTL PRPIQDILTI LCLKGPSTEG 130 140 150 160 170 180 IFRRAANEKA RKELKEELNS GDAVDLERLP VHLLAVVFKD FLRSIPRKLL SSDLFEEWMG 190 200 210 220 230 240 ALEMQDEEDR IEALKQVADK LPRPNLLLLK HLVYVLHLIS KNSEVNRMDS SNLAICIGPN 250 260 MLTLENDQSL SFEAQKDLNN KVCSAY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"T lymphocyte activation gene identification by coregulated expression on DNA microarrays." Mao M., Biery M.C., Kobayashi S.V., Ward T., Schimmack G., Burchard J., Schelter J.M., Dai H., He Y.D., Linsley P.S. Genomics 83:989-999(2004) [PubMed: 15177553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION.
[2]	"FKSG15, a novel 57 kDa protein, shares the N-terminal region with FOP." Wang Y.-G., Gong L. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Spleen.
[4]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4). Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF385429 mRNA. Translation: AAM43830.1. AF385430 mRNA. Translation: AAM43831.1. AF314817 mRNA. Translation: AAL16675.1. AK097090 mRNA. Translation: BAC04947.1. AL035530 Genomic DNA. Translation: CAI19231.1. AL035530 Genomic DNA. Translation: CAI19232.1. AL035530 Genomic DNA. Translation: CAI19233.1. BC015859 mRNA. Translation: AAH15859.1. BC111731 mRNA. Translation: AAI11732.1.
IPI	IPI00160359. IPI00166033. IPI00182659. IPI00651776.
RefSeq	NP_473455.2. NM_054114.3. NP_620165.1. NM_138810.2. NP_687034.1. NM_152133.1.
UniGene	Hs.529984.
3D structure databases
ProteinModelPortal	Q8N103.
SMR	Q8N103. Positions 83-277.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8N103.
PTM databases
PhosphoSite	Q8N103.
Polymorphism databases
DMDM	74750874.
Proteomic databases
PRIDE	Q8N103.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000367066; ENSP00000356033; ENSG00000164691.
GeneID	117289.
KEGG	hsa:117289.
UCSC	uc003qrz.1. human. uc003qsa.1. human. uc003qsb.1. human.
Organism-specific databases
CTD	117289.
GeneCards	GC06M159457.
H-InvDB	HIX0006337.
HGNC	HGNC:15669. TAGAP.
HPA	HPA031000.
MIM	609667. gene.
neXtProt	NX_Q8N103.
PharmGKB	PA38016.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG12529.
HOGENOM	HBG282610.
HOVERGEN	HBG071607.
InParanoid	Q8N103.
OMA	RRYSEPS.
OrthoDB	EOG4P8FHZ.
PhylomeDB	Q8N103.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q8N103.
Bgee	Q8N103.
CleanEx	HS_TAGAP.
Genevestigator	Q8N103.
GermOnline	ENSG00000164691. Homo sapiens.
Family and domain databases
InterPro	IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP_dom. [Graphical view]
Gene3D	G3DSA:1.10.555.10. RhoGAP. 1 hit.
Pfam	PF00620. RhoGAP. 1 hit. [Graphical view]
SMART	SM00324. RhoGAP. 1 hit. [Graphical view]
SUPFAM	SSF48350. Rho_GAP. 1 hit.
PROSITE	PS50238. RHOGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	80198.
SOURCE	Search...

Entry information

Entry name

TAGAP_HUMAN

Accession

Primary (citable) accession number: Q8N103
Secondary accession number(s): Q2NKM8 , Q8NI40, Q96KZ2, Q96QA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.