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Q8N0Z8

- PUSL1_HUMAN

UniProt

Q8N0Z8 - PUSL1_HUMAN

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Protein

tRNA pseudouridine synthase-like 1

Gene

PUSL1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

tRNA uridine = tRNA pseudouridine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661NucleophileBy similarity
Binding sitei130 – 1301SubstrateBy similarity

GO - Molecular functioni

  1. pseudouridine synthase activity Source: InterPro
  2. RNA binding Source: InterPro

GO - Biological processi

  1. pseudouridine synthesis Source: InterPro
  2. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase-like 1 (EC:5.4.99.-)
Alternative name(s):
tRNA pseudouridylate synthase-like 1
tRNA-uridine isomerase-like 1
Gene namesi
Name:PUSL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:26914. PUSL1.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134947837.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303tRNA pseudouridine synthase-like 1PRO_0000057522Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei84 – 841Phosphoserine1 Publication
Modified residuei100 – 1001Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8N0Z8.
PaxDbiQ8N0Z8.
PRIDEiQ8N0Z8.

PTM databases

PhosphoSiteiQ8N0Z8.

Expressioni

Gene expression databases

BgeeiQ8N0Z8.
CleanExiHS_PUSL1.
ExpressionAtlasiQ8N0Z8. baseline and differential.
GenevestigatoriQ8N0Z8.

Organism-specific databases

HPAiHPA032057.

Interactioni

Protein-protein interaction databases

BioGridi126015. 8 interactions.
IntActiQ8N0Z8. 1 interaction.
MINTiMINT-8052350.
STRINGi9606.ENSP00000368318.

Structurei

3D structure databases

ProteinModelPortaliQ8N0Z8.
SMRiQ8N0Z8. Positions 13-284.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0101.
GeneTreeiENSGT00510000048520.
HOGENOMiHOG000248674.
HOVERGENiHBG053770.
InParanoidiQ8N0Z8.
OMAiAHLDIQR.
OrthoDBiEOG75QR4H.
PhylomeDBiQ8N0Z8.
TreeFamiTF105127.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPiMF_00171. TruA.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
PIRSFiPIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMiSSF55120. SSF55120. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8N0Z8) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSAPASGSV RARYLVYFQY VGTDFNGVAA VRGTQRAVGV QNYLEEAAER
60 70 80 90 100
LNSVEPVRFT ISSRTDAGVH ALSNAAHLDV QRRSGRPPFP PEVLAEALNT
110 120 130 140 150
HLRHPAIRVL RAFRVPSDFH ARHAATSRTY LYRLATGCHR RDELPVFERN
160 170 180 190 200
LCWTLPADCL DMVAMQEAAQ HLLGTHDFSA FQSAGSPVPS PVRTLRRVSV
210 220 230 240 250
SPGQASPLVT PEESRKLRFW NLEFESQSFL YRQVRRMTAV LVAVGLGALA
260 270 280 290 300
PAQVKTILES QDPLGKHQTR VAPAHGLFLK SVLYGNLGAA SCTLQGPQFG

SHG
Length:303
Mass (Da):33,233
Last modified:October 1, 2002 - v1
Checksum:iA1B41615F9F214DA
GO
Isoform 2 (identifier: Q8N0Z8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-161: Missing.

Show »
Length:142
Mass (Da):15,279
Checksum:iB6C04E0C2DF4A920
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti103 – 1031R → Q.
Corresponds to variant rs12094447 [ dbSNP | Ensembl ].
VAR_034424
Natural varianti197 – 1971R → W.
Corresponds to variant rs34738574 [ dbSNP | Ensembl ].
VAR_051869

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 161161Missing in isoform 2. 1 PublicationVSP_036048Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK075292 mRNA. Translation: BAC11527.1.
AK298218 mRNA. Translation: BAG60488.1.
AL139287 Genomic DNA. Translation: CAI23172.1.
CH471183 Genomic DNA. Translation: EAW56245.1.
BC034304 mRNA. Translation: AAH34304.1.
CCDSiCCDS20.1. [Q8N0Z8-1]
RefSeqiNP_699170.1. NM_153339.1. [Q8N0Z8-1]
UniGeneiHs.400659.

Genome annotation databases

EnsembliENST00000379031; ENSP00000368318; ENSG00000169972. [Q8N0Z8-1]
GeneIDi126789.
KEGGihsa:126789.
UCSCiuc001aed.3. human. [Q8N0Z8-1]
uc010nyi.2. human. [Q8N0Z8-2]

Polymorphism databases

DMDMi52783232.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK075292 mRNA. Translation: BAC11527.1 .
AK298218 mRNA. Translation: BAG60488.1 .
AL139287 Genomic DNA. Translation: CAI23172.1 .
CH471183 Genomic DNA. Translation: EAW56245.1 .
BC034304 mRNA. Translation: AAH34304.1 .
CCDSi CCDS20.1. [Q8N0Z8-1 ]
RefSeqi NP_699170.1. NM_153339.1. [Q8N0Z8-1 ]
UniGenei Hs.400659.

3D structure databases

ProteinModelPortali Q8N0Z8.
SMRi Q8N0Z8. Positions 13-284.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 126015. 8 interactions.
IntActi Q8N0Z8. 1 interaction.
MINTi MINT-8052350.
STRINGi 9606.ENSP00000368318.

PTM databases

PhosphoSitei Q8N0Z8.

Polymorphism databases

DMDMi 52783232.

Proteomic databases

MaxQBi Q8N0Z8.
PaxDbi Q8N0Z8.
PRIDEi Q8N0Z8.

Protocols and materials databases

DNASUi 126789.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000379031 ; ENSP00000368318 ; ENSG00000169972 . [Q8N0Z8-1 ]
GeneIDi 126789.
KEGGi hsa:126789.
UCSCi uc001aed.3. human. [Q8N0Z8-1 ]
uc010nyi.2. human. [Q8N0Z8-2 ]

Organism-specific databases

CTDi 126789.
GeneCardsi GC01P001233.
HGNCi HGNC:26914. PUSL1.
HPAi HPA032057.
neXtProti NX_Q8N0Z8.
PharmGKBi PA134947837.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0101.
GeneTreei ENSGT00510000048520.
HOGENOMi HOG000248674.
HOVERGENi HBG053770.
InParanoidi Q8N0Z8.
OMAi AHLDIQR.
OrthoDBi EOG75QR4H.
PhylomeDBi Q8N0Z8.
TreeFami TF105127.

Miscellaneous databases

ChiTaRSi PUSL1. human.
GenomeRNAii 126789.
NextBioi 81916.
PROi Q8N0Z8.

Gene expression databases

Bgeei Q8N0Z8.
CleanExi HS_PUSL1.
ExpressionAtlasi Q8N0Z8. baseline and differential.
Genevestigatori Q8N0Z8.

Family and domain databases

Gene3Di 3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPi MF_00171. TruA.
InterProi IPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view ]
PANTHERi PTHR11142. PTHR11142. 1 hit.
Pfami PF01416. PseudoU_synth_1. 2 hits.
[Graphical view ]
PIRSFi PIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMi SSF55120. SSF55120. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
  2. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Skin.
  5. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-84 AND THR-100, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.

Entry informationi

Entry nameiPUSL1_HUMAN
AccessioniPrimary (citable) accession number: Q8N0Z8
Secondary accession number(s): B4DP76, Q5TA41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3