Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA pseudouridine synthase-like 1

Gene

PUSL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

tRNA uridine = tRNA pseudouridine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661NucleophileBy similarity
Binding sitei130 – 1301SubstrateBy similarity

GO - Molecular functioni

  1. pseudouridine synthase activity Source: InterPro
  2. RNA binding Source: InterPro

GO - Biological processi

  1. pseudouridine synthesis Source: InterPro
  2. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase-like 1 (EC:5.4.99.-)
Alternative name(s):
tRNA pseudouridylate synthase-like 1
tRNA-uridine isomerase-like 1
Gene namesi
Name:PUSL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:26914. PUSL1.

Subcellular locationi

GO - Cellular componenti

  1. intracellular membrane-bounded organelle Source: HPA
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134947837.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303tRNA pseudouridine synthase-like 1PRO_0000057522Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei84 – 841Phosphoserine1 Publication
Modified residuei100 – 1001Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8N0Z8.
PaxDbiQ8N0Z8.
PRIDEiQ8N0Z8.

PTM databases

PhosphoSiteiQ8N0Z8.

Expressioni

Gene expression databases

BgeeiQ8N0Z8.
CleanExiHS_PUSL1.
ExpressionAtlasiQ8N0Z8. baseline and differential.
GenevestigatoriQ8N0Z8.

Organism-specific databases

HPAiHPA032057.
HPA039999.

Interactioni

Protein-protein interaction databases

BioGridi126015. 7 interactions.
IntActiQ8N0Z8. 1 interaction.
MINTiMINT-8052350.
STRINGi9606.ENSP00000368318.

Structurei

3D structure databases

ProteinModelPortaliQ8N0Z8.
SMRiQ8N0Z8. Positions 13-284.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0101.
GeneTreeiENSGT00510000048520.
HOGENOMiHOG000248674.
HOVERGENiHBG053770.
InParanoidiQ8N0Z8.
OMAiGDIMVID.
OrthoDBiEOG75QR4H.
PhylomeDBiQ8N0Z8.
TreeFamiTF105127.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPiMF_00171. TruA.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
PIRSFiPIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMiSSF55120. SSF55120. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N0Z8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSAPASGSV RARYLVYFQY VGTDFNGVAA VRGTQRAVGV QNYLEEAAER
60 70 80 90 100
LNSVEPVRFT ISSRTDAGVH ALSNAAHLDV QRRSGRPPFP PEVLAEALNT
110 120 130 140 150
HLRHPAIRVL RAFRVPSDFH ARHAATSRTY LYRLATGCHR RDELPVFERN
160 170 180 190 200
LCWTLPADCL DMVAMQEAAQ HLLGTHDFSA FQSAGSPVPS PVRTLRRVSV
210 220 230 240 250
SPGQASPLVT PEESRKLRFW NLEFESQSFL YRQVRRMTAV LVAVGLGALA
260 270 280 290 300
PAQVKTILES QDPLGKHQTR VAPAHGLFLK SVLYGNLGAA SCTLQGPQFG

SHG
Length:303
Mass (Da):33,233
Last modified:September 30, 2002 - v1
Checksum:iA1B41615F9F214DA
GO
Isoform 2 (identifier: Q8N0Z8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-161: Missing.

Show »
Length:142
Mass (Da):15,279
Checksum:iB6C04E0C2DF4A920
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti103 – 1031R → Q.
Corresponds to variant rs12094447 [ dbSNP | Ensembl ].
VAR_034424
Natural varianti197 – 1971R → W.
Corresponds to variant rs34738574 [ dbSNP | Ensembl ].
VAR_051869

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 161161Missing in isoform 2. 1 PublicationVSP_036048Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK075292 mRNA. Translation: BAC11527.1.
AK298218 mRNA. Translation: BAG60488.1.
AL139287 Genomic DNA. Translation: CAI23172.1.
CH471183 Genomic DNA. Translation: EAW56245.1.
BC034304 mRNA. Translation: AAH34304.1.
CCDSiCCDS20.1. [Q8N0Z8-1]
RefSeqiNP_699170.1. NM_153339.1. [Q8N0Z8-1]
UniGeneiHs.400659.

Genome annotation databases

EnsembliENST00000379031; ENSP00000368318; ENSG00000169972. [Q8N0Z8-1]
GeneIDi126789.
KEGGihsa:126789.
UCSCiuc001aed.3. human. [Q8N0Z8-1]
uc010nyi.2. human. [Q8N0Z8-2]

Polymorphism databases

DMDMi52783232.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK075292 mRNA. Translation: BAC11527.1.
AK298218 mRNA. Translation: BAG60488.1.
AL139287 Genomic DNA. Translation: CAI23172.1.
CH471183 Genomic DNA. Translation: EAW56245.1.
BC034304 mRNA. Translation: AAH34304.1.
CCDSiCCDS20.1. [Q8N0Z8-1]
RefSeqiNP_699170.1. NM_153339.1. [Q8N0Z8-1]
UniGeneiHs.400659.

3D structure databases

ProteinModelPortaliQ8N0Z8.
SMRiQ8N0Z8. Positions 13-284.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126015. 7 interactions.
IntActiQ8N0Z8. 1 interaction.
MINTiMINT-8052350.
STRINGi9606.ENSP00000368318.

PTM databases

PhosphoSiteiQ8N0Z8.

Polymorphism databases

DMDMi52783232.

Proteomic databases

MaxQBiQ8N0Z8.
PaxDbiQ8N0Z8.
PRIDEiQ8N0Z8.

Protocols and materials databases

DNASUi126789.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000379031; ENSP00000368318; ENSG00000169972. [Q8N0Z8-1]
GeneIDi126789.
KEGGihsa:126789.
UCSCiuc001aed.3. human. [Q8N0Z8-1]
uc010nyi.2. human. [Q8N0Z8-2]

Organism-specific databases

CTDi126789.
GeneCardsiGC01P001233.
HGNCiHGNC:26914. PUSL1.
HPAiHPA032057.
HPA039999.
neXtProtiNX_Q8N0Z8.
PharmGKBiPA134947837.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0101.
GeneTreeiENSGT00510000048520.
HOGENOMiHOG000248674.
HOVERGENiHBG053770.
InParanoidiQ8N0Z8.
OMAiGDIMVID.
OrthoDBiEOG75QR4H.
PhylomeDBiQ8N0Z8.
TreeFamiTF105127.

Miscellaneous databases

ChiTaRSiPUSL1. human.
GenomeRNAii126789.
NextBioi81916.
PROiQ8N0Z8.

Gene expression databases

BgeeiQ8N0Z8.
CleanExiHS_PUSL1.
ExpressionAtlasiQ8N0Z8. baseline and differential.
GenevestigatoriQ8N0Z8.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPiMF_00171. TruA.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
PIRSFiPIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMiSSF55120. SSF55120. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
  2. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Skin.
  5. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-84 AND THR-100, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.

Entry informationi

Entry nameiPUSL1_HUMAN
AccessioniPrimary (citable) accession number: Q8N0Z8
Secondary accession number(s): B4DP76, Q5TA41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2004
Last sequence update: September 30, 2002
Last modified: March 3, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.