Q8N0Z8 (PUSL1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase-like 1
EC=5.4.99.-

Alternative name(s):
tRNA pseudouridylate synthase-like 1
tRNA-uridine isomerase-like 1

Gene names

Name:

PUSL1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	tRNA uridine = tRNA pseudouridine.
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
Biological process	tRNA processing
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Isomerase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N0Z8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N0Z8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-161: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	tRNA pseudouridine synthase-like 1		PRO_0000057522
Sites
	Active site	66	1	Nucleophile By similarity
	Binding site	130	1	Substrate By similarity
Amino acid modifications
	Modified residue	84	1	Phosphoserine Ref.5
	Modified residue	100	1	Phosphothreonine Ref.5
Natural variations
	Alternative sequence	1 – 161	161	Missing in isoform 2.		VSP_036048
	Natural variant	103	1	R → Q. Corresponds to variant rs12094447 [ dbSNP | Ensembl ].		VAR_034424
	Natural variant	197	1	R → W. Corresponds to variant rs34738574 [ dbSNP | Ensembl ].		VAR_051869

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: A1B41615F9F214DA	FASTA	303	33,233
	10 20 30 40 50 60 MSSAPASGSV RARYLVYFQY VGTDFNGVAA VRGTQRAVGV QNYLEEAAER LNSVEPVRFT 70 80 90 100 110 120 ISSRTDAGVH ALSNAAHLDV QRRSGRPPFP PEVLAEALNT HLRHPAIRVL RAFRVPSDFH 130 140 150 160 170 180 ARHAATSRTY LYRLATGCHR RDELPVFERN LCWTLPADCL DMVAMQEAAQ HLLGTHDFSA 190 200 210 220 230 240 FQSAGSPVPS PVRTLRRVSV SPGQASPLVT PEESRKLRFW NLEFESQSFL YRQVRRMTAV 250 260 270 280 290 300 LVAVGLGALA PAQVKTILES QDPLGKHQTR VAPAHGLFLK SVLYGNLGAA SCTLQGPQFG SHG
	Isoform 2 [UniParc]. Checksum: B6C04E0C2DF4A920	FASTA	142	15,279
	10 20 30 40 50 60 MVAMQEAAQH LLGTHDFSAF QSAGSPVPSP VRTLRRVSVS PGQASPLVTP EESRKLRFWN 70 80 90 100 110 120 LEFESQSFLY RQVRRMTAVL VAVGLGALAP AQVKTILESQ DPLGKHQTRV APAHGLFLKS 130 140 VLYGNLGAAS CTLQGPQFGS HG

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Skin.
[5]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-84 AND THR-100, MASS SPECTROMETRY. Tissue: Leukemic T-cell.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK075292 mRNA. Translation: BAC11527.1. AK298218 mRNA. Translation: BAG60488.1. AL139287 Genomic DNA. Translation: CAI23172.1. CH471183 Genomic DNA. Translation: EAW56245.1. BC034304 mRNA. Translation: AAH34304.1.
IPI	IPI00166125. IPI00915388.
RefSeq	NP_699170.1. NM_153339.1.
UniGene	Hs.400659.
3D structure databases
ProteinModelPortal	Q8N0Z8.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000368318.
PTM databases
PhosphoSite	Q8N0Z8.
Polymorphism databases
DMDM	52783232.
Proteomic databases
PaxDb	Q8N0Z8.
PRIDE	Q8N0Z8.
Protocols and materials databases
DNASU	126789.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000379031; ENSP00000368318; ENSG00000169972.
GeneID	126789.
KEGG	hsa:126789.
UCSC	uc001aed.3. human. uc010nyi.2. human.
Organism-specific databases
CTD	126789.
GeneCards	GC01P001233.
HGNC	HGNC:26914. PUSL1.
HPA	HPA032057.
neXtProt	NX_Q8N0Z8.
PharmGKB	PA134947837.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0101.
HOGENOM	HOG000248674.
HOVERGEN	HBG053770.
InParanoid	Q8N0Z8.
OMA	WALPADC.
OrthoDB	EOG4P5K9S.
PhylomeDB	Q8N0Z8.
Gene expression databases
Bgee	Q8N0Z8.
CleanEx	HS_PUSL1.
Genevestigator	Q8N0Z8.
GermOnline	ENSG00000169972. Homo sapiens.
Family and domain databases
Gene3D	3.30.70.580. 1 hit. 3.30.70.660. 1 hit.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
PANTHER	PTHR11142. PTHR11142. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	PUSL1. human.
GenomeRNAi	126789.
NextBio	81916.

Entry information

Entry name

PUSL1_HUMAN

Accession

Primary (citable) accession number: Q8N0Z8
Secondary accession number(s): B4DP76, Q5TA41

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.