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Q8N0Z8

- PUSL1_HUMAN

UniProt

Q8N0Z8 - PUSL1_HUMAN

Protein

tRNA pseudouridine synthase-like 1

Gene

PUSL1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    tRNA uridine = tRNA pseudouridine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei66 – 661NucleophileBy similarity
    Binding sitei130 – 1301SubstrateBy similarity

    GO - Molecular functioni

    1. pseudouridine synthase activity Source: InterPro
    2. RNA binding Source: InterPro

    GO - Biological processi

    1. pseudouridine synthesis Source: InterPro
    2. tRNA processing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    tRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA pseudouridine synthase-like 1 (EC:5.4.99.-)
    Alternative name(s):
    tRNA pseudouridylate synthase-like 1
    tRNA-uridine isomerase-like 1
    Gene namesi
    Name:PUSL1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:26914. PUSL1.

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134947837.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 303303tRNA pseudouridine synthase-like 1PRO_0000057522Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei84 – 841Phosphoserine1 Publication
    Modified residuei100 – 1001Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8N0Z8.
    PaxDbiQ8N0Z8.
    PRIDEiQ8N0Z8.

    PTM databases

    PhosphoSiteiQ8N0Z8.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8N0Z8.
    BgeeiQ8N0Z8.
    CleanExiHS_PUSL1.
    GenevestigatoriQ8N0Z8.

    Organism-specific databases

    HPAiHPA032057.

    Interactioni

    Protein-protein interaction databases

    BioGridi126015. 4 interactions.
    IntActiQ8N0Z8. 1 interaction.
    MINTiMINT-8052350.
    STRINGi9606.ENSP00000368318.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8N0Z8.
    SMRiQ8N0Z8. Positions 13-284.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0101.
    HOGENOMiHOG000248674.
    HOVERGENiHBG053770.
    InParanoidiQ8N0Z8.
    OMAiAHLDIQR.
    OrthoDBiEOG75QR4H.
    PhylomeDBiQ8N0Z8.
    TreeFamiTF105127.

    Family and domain databases

    Gene3Di3.30.70.580. 1 hit.
    3.30.70.660. 1 hit.
    HAMAPiMF_00171. TruA.
    InterProiIPR020103. PsdUridine_synth_cat_dom.
    IPR001406. PsdUridine_synth_TruA.
    IPR020097. PsdUridine_synth_TruA_a/b_dom.
    IPR020095. PsdUridine_synth_TruA_C.
    IPR020094. PsdUridine_synth_TruA_N.
    [Graphical view]
    PANTHERiPTHR11142. PTHR11142. 1 hit.
    PfamiPF01416. PseudoU_synth_1. 2 hits.
    [Graphical view]
    PIRSFiPIRSF001430. tRNA_psdUrid_synth. 1 hit.
    SUPFAMiSSF55120. SSF55120. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8N0Z8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSAPASGSV RARYLVYFQY VGTDFNGVAA VRGTQRAVGV QNYLEEAAER    50
    LNSVEPVRFT ISSRTDAGVH ALSNAAHLDV QRRSGRPPFP PEVLAEALNT 100
    HLRHPAIRVL RAFRVPSDFH ARHAATSRTY LYRLATGCHR RDELPVFERN 150
    LCWTLPADCL DMVAMQEAAQ HLLGTHDFSA FQSAGSPVPS PVRTLRRVSV 200
    SPGQASPLVT PEESRKLRFW NLEFESQSFL YRQVRRMTAV LVAVGLGALA 250
    PAQVKTILES QDPLGKHQTR VAPAHGLFLK SVLYGNLGAA SCTLQGPQFG 300
    SHG 303
    Length:303
    Mass (Da):33,233
    Last modified:October 1, 2002 - v1
    Checksum:iA1B41615F9F214DA
    GO
    Isoform 2 (identifier: Q8N0Z8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-161: Missing.

    Show »
    Length:142
    Mass (Da):15,279
    Checksum:iB6C04E0C2DF4A920
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti103 – 1031R → Q.
    Corresponds to variant rs12094447 [ dbSNP | Ensembl ].
    VAR_034424
    Natural varianti197 – 1971R → W.
    Corresponds to variant rs34738574 [ dbSNP | Ensembl ].
    VAR_051869

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 161161Missing in isoform 2. 1 PublicationVSP_036048Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK075292 mRNA. Translation: BAC11527.1.
    AK298218 mRNA. Translation: BAG60488.1.
    AL139287 Genomic DNA. Translation: CAI23172.1.
    CH471183 Genomic DNA. Translation: EAW56245.1.
    BC034304 mRNA. Translation: AAH34304.1.
    CCDSiCCDS20.1. [Q8N0Z8-1]
    RefSeqiNP_699170.1. NM_153339.1. [Q8N0Z8-1]
    UniGeneiHs.400659.

    Genome annotation databases

    EnsembliENST00000379031; ENSP00000368318; ENSG00000169972. [Q8N0Z8-1]
    GeneIDi126789.
    KEGGihsa:126789.
    UCSCiuc001aed.3. human. [Q8N0Z8-1]
    uc010nyi.2. human. [Q8N0Z8-2]

    Polymorphism databases

    DMDMi52783232.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK075292 mRNA. Translation: BAC11527.1 .
    AK298218 mRNA. Translation: BAG60488.1 .
    AL139287 Genomic DNA. Translation: CAI23172.1 .
    CH471183 Genomic DNA. Translation: EAW56245.1 .
    BC034304 mRNA. Translation: AAH34304.1 .
    CCDSi CCDS20.1. [Q8N0Z8-1 ]
    RefSeqi NP_699170.1. NM_153339.1. [Q8N0Z8-1 ]
    UniGenei Hs.400659.

    3D structure databases

    ProteinModelPortali Q8N0Z8.
    SMRi Q8N0Z8. Positions 13-284.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 126015. 4 interactions.
    IntActi Q8N0Z8. 1 interaction.
    MINTi MINT-8052350.
    STRINGi 9606.ENSP00000368318.

    PTM databases

    PhosphoSitei Q8N0Z8.

    Polymorphism databases

    DMDMi 52783232.

    Proteomic databases

    MaxQBi Q8N0Z8.
    PaxDbi Q8N0Z8.
    PRIDEi Q8N0Z8.

    Protocols and materials databases

    DNASUi 126789.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000379031 ; ENSP00000368318 ; ENSG00000169972 . [Q8N0Z8-1 ]
    GeneIDi 126789.
    KEGGi hsa:126789.
    UCSCi uc001aed.3. human. [Q8N0Z8-1 ]
    uc010nyi.2. human. [Q8N0Z8-2 ]

    Organism-specific databases

    CTDi 126789.
    GeneCardsi GC01P001233.
    HGNCi HGNC:26914. PUSL1.
    HPAi HPA032057.
    neXtProti NX_Q8N0Z8.
    PharmGKBi PA134947837.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0101.
    HOGENOMi HOG000248674.
    HOVERGENi HBG053770.
    InParanoidi Q8N0Z8.
    OMAi AHLDIQR.
    OrthoDBi EOG75QR4H.
    PhylomeDBi Q8N0Z8.
    TreeFami TF105127.

    Miscellaneous databases

    ChiTaRSi PUSL1. human.
    GenomeRNAii 126789.
    NextBioi 81916.
    PROi Q8N0Z8.

    Gene expression databases

    ArrayExpressi Q8N0Z8.
    Bgeei Q8N0Z8.
    CleanExi HS_PUSL1.
    Genevestigatori Q8N0Z8.

    Family and domain databases

    Gene3Di 3.30.70.580. 1 hit.
    3.30.70.660. 1 hit.
    HAMAPi MF_00171. TruA.
    InterProi IPR020103. PsdUridine_synth_cat_dom.
    IPR001406. PsdUridine_synth_TruA.
    IPR020097. PsdUridine_synth_TruA_a/b_dom.
    IPR020095. PsdUridine_synth_TruA_C.
    IPR020094. PsdUridine_synth_TruA_N.
    [Graphical view ]
    PANTHERi PTHR11142. PTHR11142. 1 hit.
    Pfami PF01416. PseudoU_synth_1. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF001430. tRNA_psdUrid_synth. 1 hit.
    SUPFAMi SSF55120. SSF55120. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    2. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Skin.
    5. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-84 AND THR-100, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.

    Entry informationi

    Entry nameiPUSL1_HUMAN
    AccessioniPrimary (citable) accession number: Q8N0Z8
    Secondary accession number(s): B4DP76, Q5TA41
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2004
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3