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Protein

Zinc finger protein 444

Gene

ZNF444

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator. Binds to the 5'-flanking critical region of the SCARF1 promoter.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri179 – 20123C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri207 – 22923C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri250 – 27223C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri278 – 30023C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 444
Alternative name(s):
Endothelial zinc finger protein 2
Short name:
EZF-2
Zinc finger and SCAN domain-containing protein 17
Gene namesi
Name:ZNF444
Synonyms:EZF2, ZSCAN17
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:16052. ZNF444.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134984823.

Polymorphism and mutation databases

BioMutaiZNF444.
DMDMi38258800.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327Zinc finger protein 444PRO_0000047593Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources
Modified residuei18 – 181PhosphoserineCombined sources
Modified residuei104 – 1041PhosphoserineCombined sources
Modified residuei235 – 2351PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8N0Y2.
MaxQBiQ8N0Y2.
PaxDbiQ8N0Y2.
PRIDEiQ8N0Y2.

PTM databases

iPTMnetiQ8N0Y2.
PhosphoSiteiQ8N0Y2.

Expressioni

Gene expression databases

BgeeiQ8N0Y2.
CleanExiHS_ZNF444.
ExpressionAtlasiQ8N0Y2. baseline and differential.
GenevisibleiQ8N0Y2. HS.

Organism-specific databases

HPAiHPA056895.

Interactioni

Protein-protein interaction databases

BioGridi120593. 34 interactions.
IntActiQ8N0Y2. 2 interactions.
STRINGi9606.ENSP00000338860.

Structurei

3D structure databases

ProteinModelPortaliQ8N0Y2.
SMRiQ8N0Y2. Positions 22-98, 181-309.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 10485SCAN boxPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 SCAN box domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri179 – 20123C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri207 – 22923C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri250 – 27223C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri278 – 30023C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00840000129892.
HOGENOMiHOG000234619.
HOVERGENiHBG018163.
InParanoidiQ8N0Y2.
KOiK09230.
OMAiVCSRQPQ.
PhylomeDBiQ8N0Y2.
TreeFamiTF337369.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR027775. C2H2_Znf_fam.
IPR008916. Retrov_capsid_C.
IPR003309. SCAN_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PANTHERiPTHR10032. PTHR10032. 1 hit.
PfamiPF02023. SCAN. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00431. SCAN. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view]
SUPFAMiSSF47353. SSF47353. 1 hit.
PROSITEiPS50804. SCAN_BOX. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8N0Y2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEVAVPVKQE AEGLALDSPW HRFRRFHLGD APGPREALGL LRALCRDWLR
60 70 80 90 100
PEVHTKEQML ELLVLEQFLS ALPADTQAWV CSRQPQSGEE AVALLEELWG
110 120 130 140 150
PAASPDGSSA TRVPQDVTQG PGATGGKEDS GMIPLAGTAP GAEGPAPGDS
160 170 180 190 200
QAVRPYKQEP SSPPLAPGLP AFLAAPGTTS CPECGKTSLK PAHLLRHRQS
210 220 230 240 250
HSGEKPHACP ECGKAFRRKE HLRRHRDTHP GSPGSPGPAL RPLPAREKPH
260 270 280 290 300
ACCECGKTFY WREHLVRHRK THSGARPFAC WECGKGFGRR EHVLRHQRIH
310 320
GRAAASAQGA VAPGPDGGGP FPPWPLG
Length:327
Mass (Da):35,204
Last modified:October 1, 2002 - v1
Checksum:iC7C812DC8FC6D706
GO
Isoform 2 (identifier: Q8N0Y2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     136-136: Missing.

Show »
Length:326
Mass (Da):35,133
Checksum:i4F3E49DD9F6182AF
GO

Sequence cautioni

The sequence BAB84889.1 differs from that shown. Reason: Frameshift at position 99. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491L → P in BAA92028 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei136 – 1361Missing in isoform 2. 3 PublicationsVSP_008798

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052955 mRNA. Translation: BAC02698.1.
AB052954 mRNA. Translation: BAC02697.1.
AL834130 mRNA. Translation: CAD38847.1.
AK074063 mRNA. Translation: BAB84889.1. Frameshift.
AK001999 mRNA. Translation: BAA92028.1.
BC021282 mRNA. Translation: AAH21282.1.
CCDSiCCDS12939.1. [Q8N0Y2-1]
CCDS59426.1. [Q8N0Y2-2]
RefSeqiNP_001240721.1. NM_001253792.1. [Q8N0Y2-2]
NP_060807.2. NM_018337.3. [Q8N0Y2-1]
XP_005259091.1. XM_005259034.2. [Q8N0Y2-1]
XP_005259092.1. XM_005259035.1. [Q8N0Y2-1]
XP_005259093.1. XM_005259036.1. [Q8N0Y2-1]
XP_011525370.1. XM_011527068.1. [Q8N0Y2-1]
UniGeneiHs.24545.
Hs.741890.

Genome annotation databases

EnsembliENST00000337080; ENSP00000338860; ENSG00000167685. [Q8N0Y2-1]
ENST00000592949; ENSP00000468069; ENSG00000167685. [Q8N0Y2-2]
GeneIDi55311.
KEGGihsa:55311.
UCSCiuc002qmm.4. human. [Q8N0Y2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052955 mRNA. Translation: BAC02698.1.
AB052954 mRNA. Translation: BAC02697.1.
AL834130 mRNA. Translation: CAD38847.1.
AK074063 mRNA. Translation: BAB84889.1. Frameshift.
AK001999 mRNA. Translation: BAA92028.1.
BC021282 mRNA. Translation: AAH21282.1.
CCDSiCCDS12939.1. [Q8N0Y2-1]
CCDS59426.1. [Q8N0Y2-2]
RefSeqiNP_001240721.1. NM_001253792.1. [Q8N0Y2-2]
NP_060807.2. NM_018337.3. [Q8N0Y2-1]
XP_005259091.1. XM_005259034.2. [Q8N0Y2-1]
XP_005259092.1. XM_005259035.1. [Q8N0Y2-1]
XP_005259093.1. XM_005259036.1. [Q8N0Y2-1]
XP_011525370.1. XM_011527068.1. [Q8N0Y2-1]
UniGeneiHs.24545.
Hs.741890.

3D structure databases

ProteinModelPortaliQ8N0Y2.
SMRiQ8N0Y2. Positions 22-98, 181-309.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120593. 34 interactions.
IntActiQ8N0Y2. 2 interactions.
STRINGi9606.ENSP00000338860.

PTM databases

iPTMnetiQ8N0Y2.
PhosphoSiteiQ8N0Y2.

Polymorphism and mutation databases

BioMutaiZNF444.
DMDMi38258800.

Proteomic databases

EPDiQ8N0Y2.
MaxQBiQ8N0Y2.
PaxDbiQ8N0Y2.
PRIDEiQ8N0Y2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000337080; ENSP00000338860; ENSG00000167685. [Q8N0Y2-1]
ENST00000592949; ENSP00000468069; ENSG00000167685. [Q8N0Y2-2]
GeneIDi55311.
KEGGihsa:55311.
UCSCiuc002qmm.4. human. [Q8N0Y2-1]

Organism-specific databases

CTDi55311.
GeneCardsiZNF444.
HGNCiHGNC:16052. ZNF444.
HPAiHPA056895.
MIMi607874. gene.
neXtProtiNX_Q8N0Y2.
PharmGKBiPA134984823.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00840000129892.
HOGENOMiHOG000234619.
HOVERGENiHBG018163.
InParanoidiQ8N0Y2.
KOiK09230.
OMAiVCSRQPQ.
PhylomeDBiQ8N0Y2.
TreeFamiTF337369.

Miscellaneous databases

GenomeRNAii55311.
PROiQ8N0Y2.
SOURCEiSearch...

Gene expression databases

BgeeiQ8N0Y2.
CleanExiHS_ZNF444.
ExpressionAtlasiQ8N0Y2. baseline and differential.
GenevisibleiQ8N0Y2. HS.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR027775. C2H2_Znf_fam.
IPR008916. Retrov_capsid_C.
IPR003309. SCAN_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PANTHERiPTHR10032. PTHR10032. 1 hit.
PfamiPF02023. SCAN. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00431. SCAN. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view]
SUPFAMiSSF47353. SSF47353. 1 hit.
PROSITEiPS50804. SCAN_BOX. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the human gene encoding the scavenger receptor expressed by endothelial cell and its regulation by a novel transcription factor, endothelial zinc finger protein-2."
    Adachi H., Tsujimoto M.
    J. Biol. Chem. 277:24014-24021(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Uterus.
  3. "The nucleotide sequence of a long cDNA clone isolated from human spleen."
    Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Spleen.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Placenta.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Lung.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-235, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiZN444_HUMAN
AccessioniPrimary (citable) accession number: Q8N0Y2
Secondary accession number(s): Q8TEQ9, Q8WU35, Q9NUU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: October 1, 2002
Last modified: June 8, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.