Q8N0X4 (CLYBL_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Citrate lyase subunit beta-like protein, mitochondrial
Short name=Citrate lyase beta-like
EC=4.1.-.-

Gene names

Name:	CLYBL
Synonyms:	CLB

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subcellular location	Mitochondrion Potential.
Sequence similarities	Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit-like subfamily.
Caution	This organism lacks the other subunits that are necessary for ATP-independent citrate lyase activity. Even though this protein has clear similarity to citrate lyase beta subunit, it is expected to have a somewhat different enzyme activity.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transit peptide
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular aromatic compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	citrate lyase complex Inferred from electronic annotation. Source: InterPro mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	citrate (pro-3S)-lyase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8N0X4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8N0X4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     147-180: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 22	22	Mitochondrion Potential
	Chain	23 – 340	318	Citrate lyase subunit beta-like protein, mitochondrial		PRO_0000286389
Sites
	Metal binding	171	1	Magnesium By similarity
	Metal binding	206	1	Magnesium By similarity
	Binding site	107	1	Substrate By similarity
	Binding site	171	1	Substrate By similarity
Natural variations
	Alternative sequence	147 – 180	34	Missing in isoform 2.		VSP_034643
	Natural variant	28	1	D → Y. Corresponds to variant rs17577293 [ dbSNP | Ensembl ].		VAR_032099
	Natural variant	128	1	V → I. Corresponds to variant rs35680839 [ dbSNP | Ensembl ].		VAR_032100
	Natural variant	241	1	I → V. Ref.2 Ref.4 Corresponds to variant rs3783185 [ dbSNP | Ensembl ].		VAR_032101

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2007. Version 2. Checksum: 0C99A5A4F09E03E2	FASTA	340	37,359
	10 20 30 40 50 60 MALRLLRRAA RGAAAAALLR LKASLAADIP RLGYSSSSHH KYIPRRAVLY VPGNDEKKIK 70 80 90 100 110 120 KIPSLNVDCA VLDCEDGVAA NKKNEARLRI VKTLEDIDLG PTEKCVRVNS VSSGLAEEDL 130 140 150 160 170 180 ETLLQSRVLP SSLMLPKVES PEEIQWFADK FSFHLKGRKL EQPMNLIPFV ETAMGLLNFK 190 200 210 220 230 240 AVCEETLKVG PQVGLFLDAV VFGGEDFRAS IGATSSKETL DILYARQKIV VIAKAFGLQA 250 260 270 280 290 300 IDLVYIDFRD GAGLLRQSRE GAAMGFTGKQ VIHPNQIAVV QEQFSPSPEK IKWAEELIAA 310 320 330 340 FKEHQQLGKG AFTFQGSMID MPLLKQAQNT VTLATSIKEK
	Isoform 2 [UniParc]. Checksum: EDFEA04BA12E1934	FASTA	306	33,409
	10 20 30 40 50 60 MALRLLRRAA RGAAAAALLR LKASLAADIP RLGYSSSSHH KYIPRRAVLY VPGNDEKKIK 70 80 90 100 110 120 KIPSLNVDCA VLDCEDGVAA NKKNEARLRI VKTLEDIDLG PTEKCVRVNS VSSGLAEEDL 130 140 150 160 170 180 ETLLQSRVLP SSLMLPKVES PEEIQWAVCE ETLKVGPQVG LFLDAVVFGG EDFRASIGAT 190 200 210 220 230 240 SSKETLDILY ARQKIVVIAK AFGLQAIDLV YIDFRDGAGL LRQSREGAAM GFTGKQVIHP 250 260 270 280 290 300 NQIAVVQEQF SPSPEKIKWA EELIAAFKEH QQLGKGAFTF QGSMIDMPLL KQAQNTVTLA TSIKEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of novel mouse and human putative citrate lyase beta-subunit." Morikawa J., Nishimura Y., Uchida A., Tanaka T. Biochem. Biophys. Res. Commun. 289:1282-1286(2001) [PubMed: 11741334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT VAL-241. Tissue: Brain.
[3]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed: 15057823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT VAL-241. Tissue: Skin.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF428253 mRNA. Translation: AAL84703.1. AK095506 mRNA. Translation: BAC04561.1. AL137139, AL139035 Genomic DNA. Translation: CAI39520.1. AL139035, AL137139 Genomic DNA. Translation: CAH71385.1. AL139035, AL137139 Genomic DNA. Translation: CAH71384.1. AL137139, AL139035 Genomic DNA. Translation: CAI39521.1. BC034360 mRNA. Translation: AAH34360.1.
IPI	IPI00477957. IPI00641200.
RefSeq	NP_996531.1. NM_206808.2.
UniGene	Hs.655642.
3D structure databases
ProteinModelPortal	Q8N0X4.
SMR	Q8N0X4. Positions 42-285.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8N0X4.
PTM databases
PhosphoSite	Q8N0X4.
Polymorphism databases
DMDM	146286073.
Proteomic databases
PRIDE	Q8N0X4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000339105; ENSP00000342991; ENSG00000125246. ENST00000376360; ENSP00000365538; ENSG00000125246.
GeneID	171425.
KEGG	hsa:171425.
UCSC	uc001vok.1. human.
Organism-specific databases
CTD	171425.
GeneCards	GC13P100258.
H-InvDB	HIX0011427.
HGNC	HGNC:18355. CLYBL.
HPA	HPA039959. HPA040691.
MIM	609686. gene.
neXtProt	NX_Q8N0X4.
PharmGKB	PA26622.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00390000017163.
HOGENOM	HBG676713.
HOVERGEN	HBG059382.
InParanoid	Q8N0X4.
OMA	YIDFRDE.
OrthoDB	EOG498V15.
PhylomeDB	Q8N0X4.
Gene expression databases
ArrayExpress	Q8N0X4.
Bgee	Q8N0X4.
CleanEx	HS_CLYBL.
Genevestigator	Q8N0X4.
Family and domain databases
InterPro	IPR005000. Aldehyde-lyase_domain. IPR011206. Citrate_lyase_beta. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
KO	K11390.
PANTHER	PTHR11105. PTHR11105. 1 hit.
Pfam	PF03328. HpcH_HpaI. 1 hit. [Graphical view]
PIRSF	PIRSF015582. Cit_lyase_B. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
ProtoNet	Search...
Other
NextBio	89246.
SOURCE	Search...

Entry information

Entry name

CLYBL_HUMAN

Accession

Primary (citable) accession number: Q8N0X4
Secondary accession number(s): Q5W0F7, Q8TDH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	May 1, 2007
Last modified:	January 25, 2012
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.