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Protein

Mitogen-activated protein kinase pmk-2

Gene

pmk-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+1 Publication

Enzyme regulationi

Activated by phosphorylation on threonine and tyrosine. Inhibited by pyridinyl-imidazole related compounds.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78ATPPROSITE-ProRule annotation1
Active sitei210Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi55 – 63ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: UniProtKB

GO - Biological processi

  • intracellular signal transduction Source: UniProtKB
  • response to osmotic stress Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-168638. NOD1/2 Signaling Pathway.
R-CEL-171007. p38MAPK events.
R-CEL-198753. ERK/MAPK targets.
R-CEL-2559580. Oxidative Stress Induced Senescence.
R-CEL-375170. CDO in myogenesis.
R-CEL-418592. ADP signalling through P2Y purinoceptor 1.
R-CEL-432142. Platelet sensitization by LDL.
R-CEL-4420097. VEGFA-VEGFR2 Pathway.
R-CEL-450302. activated TAK1 mediates p38 MAPK activation.
R-CEL-6798695. Neutrophil degranulation.
SignaLinkiQ8MXI4.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase pmk-2 (EC:2.7.11.24)
Alternative name(s):
Stress-activated protein kinase pmk-2
p38 MAP kinase 2
Gene namesi
Name:pmk-2
ORF Names:F42G8.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiF42G8.3a; CE49565; WBGene00004056; pmk-2.
F42G8.3b; CE34862; WBGene00004056; pmk-2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863041 – 419Mitogen-activated protein kinase pmk-2Add BLAST419

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei222PhosphothreonineBy similarity1
Modified residuei224PhosphotyrosineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-222 and Tyr-224, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8MXI4.
PeptideAtlasiQ8MXI4.
PRIDEiQ8MXI4.

Expressioni

Gene expression databases

BgeeiWBGene00004056.
ExpressionAtlasiQ8MXI4. differential.

Interactioni

Protein-protein interaction databases

BioGridi42724. 2 interactors.
DIPiDIP-24927N.
IntActiQ8MXI4. 1 interactor.
MINTiMINT-1042906.
STRINGi6239.F42G8.3a.2.

Structurei

3D structure databases

ProteinModelPortaliQ8MXI4.
SMRiQ8MXI4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 350Protein kinasePROSITE-ProRule annotationAdd BLAST302

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi222 – 224TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ8MXI4.
KOiK04441.
OMAiQSTIHAK.
OrthoDBiEOG091G08QL.
PhylomeDBiQ8MXI4.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform aCurated (identifier: Q8MXI4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGMSATMGDS ASIPGVFFAD FGPAPPEITP EGYHEVELNK TKWVLPQWYN
60 70 80 90 100
SLKPLGEGAY GVVCTAEYEP TGDRVAIKKF FRPFQSTIHA KRTYRELKLL
110 120 130 140 150
RTLQHDNVLE MIDVFTPDPD ASSLNNVYFV SVLMGSDLQN IMKIQRLTDE
160 170 180 190 200
QIQLLIYQVL RGLKYIHSAG IIHRDLKPSN IAVNERCEVK VFLSFSQLSF
210 220 230 240 250
LILSFFKILD FGLARAQDAE MTGYVATRWY RAPEIMLNWM HYTQTVDVWS
260 270 280 290 300
VGCILAELVS GRPLFPGDDH IDQLTKIMSV VGTPKEEFWS KIQSEEARNY
310 320 330 340 350
IKNRSPIIRQ DFVTLFPMAS PYALELLEMM LILDPDRRIS VSSALRHDYL
360 370 380 390 400
REYSVPNDEP VAMDTVINSI VTIDPAEERA TTLSDWRELI WNEIRLFQNS
410
ARRLSFVSCT DTEEEPMKI
Length:419
Mass (Da):48,026
Last modified:October 1, 2002 - v1
Checksum:i621C52BFAB7EF37B
GO
Isoform bCurated (identifier: Q8MXI4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     190-206: Missing.
     366-370: Missing.

Note: No experimental confirmation available.
Show »
Length:397
Mass (Da):45,494
Checksum:iE9393A7F1AFAC2E2
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009272190 – 206Missing in isoform b. CuratedAdd BLAST17
Alternative sequenceiVSP_050273366 – 370Missing in isoform b. Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080126 Genomic DNA. Translation: CCD61402.1.
FO080126 Genomic DNA. Translation: CCD61403.1.
RefSeqiNP_741457.2. NM_171392.5.
NP_741458.2. NM_171913.4. [Q8MXI4-2]
UniGeneiCel.7053.

Genome annotation databases

GeneIDi177611.
KEGGicel:CELE_F42G8.3.
UCSCiF42G8.3a. c. elegans. [Q8MXI4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080126 Genomic DNA. Translation: CCD61402.1.
FO080126 Genomic DNA. Translation: CCD61403.1.
RefSeqiNP_741457.2. NM_171392.5.
NP_741458.2. NM_171913.4. [Q8MXI4-2]
UniGeneiCel.7053.

3D structure databases

ProteinModelPortaliQ8MXI4.
SMRiQ8MXI4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi42724. 2 interactors.
DIPiDIP-24927N.
IntActiQ8MXI4. 1 interactor.
MINTiMINT-1042906.
STRINGi6239.F42G8.3a.2.

Proteomic databases

PaxDbiQ8MXI4.
PeptideAtlasiQ8MXI4.
PRIDEiQ8MXI4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi177611.
KEGGicel:CELE_F42G8.3.
UCSCiF42G8.3a. c. elegans. [Q8MXI4-1]

Organism-specific databases

CTDi177611.
WormBaseiF42G8.3a; CE49565; WBGene00004056; pmk-2.
F42G8.3b; CE34862; WBGene00004056; pmk-2.

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ8MXI4.
KOiK04441.
OMAiQSTIHAK.
OrthoDBiEOG091G08QL.
PhylomeDBiQ8MXI4.

Enzyme and pathway databases

ReactomeiR-CEL-168638. NOD1/2 Signaling Pathway.
R-CEL-171007. p38MAPK events.
R-CEL-198753. ERK/MAPK targets.
R-CEL-2559580. Oxidative Stress Induced Senescence.
R-CEL-375170. CDO in myogenesis.
R-CEL-418592. ADP signalling through P2Y purinoceptor 1.
R-CEL-432142. Platelet sensitization by LDL.
R-CEL-4420097. VEGFA-VEGFR2 Pathway.
R-CEL-450302. activated TAK1 mediates p38 MAPK activation.
R-CEL-6798695. Neutrophil degranulation.
SignaLinkiQ8MXI4.

Miscellaneous databases

PROiQ8MXI4.

Gene expression databases

BgeeiWBGene00004056.
ExpressionAtlasiQ8MXI4. differential.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPMK2_CAEEL
AccessioniPrimary (citable) accession number: Q8MXI4
Secondary accession number(s): Q8MXI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.