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Q8MXI4 (PMK2_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase pmk-2

EC=2.7.11.24
Alternative name(s):
Stress-activated protein kinase pmk-2
p38 MAP kinase 2
Gene names
Name:pmk-2
ORF Names:F42G8.3
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length419 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. Ref.1

Cofactor

Magnesium. Ref.1

Enzyme regulation

Activated by phosphorylation on threonine and tyrosine. Inhibited by pyridinyl-imidazole related compounds. Ref.1

Subcellular location

Cytoplasm Ref.1.

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-222 and Tyr-224, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processMAPK cascade

Inferred from direct assay Ref.1. Source: GOC

intracellular signal transduction

Inferred from direct assay Ref.1. Source: UniProtKB

response to osmotic stress

Inferred from direct assay Ref.1. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

MAP kinase activity

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: Q8MXI4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: Q8MXI4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     190-206: Missing.
     366-370: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 419419Mitogen-activated protein kinase pmk-2
PRO_0000186304

Regions

Domain49 – 350302Protein kinase
Nucleotide binding55 – 639ATP By similarity UniProtKB Q16539
Motif222 – 2243TXY

Sites

Active site2101Proton acceptor By similarity UniProtKB Q16539
Binding site781ATP By similarity UniProtKB Q16539

Amino acid modifications

Modified residue2221Phosphothreonine By similarity UniProtKB Q16539
Modified residue2241Phosphotyrosine By similarity UniProtKB Q16539

Natural variations

Alternative sequence190 – 20617Missing in isoform b.
VSP_009272
Alternative sequence366 – 3705Missing in isoform b.
VSP_050273

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 621C52BFAB7EF37B

FASTA41948,026
        10         20         30         40         50         60 
MGMSATMGDS ASIPGVFFAD FGPAPPEITP EGYHEVELNK TKWVLPQWYN SLKPLGEGAY 

        70         80         90        100        110        120 
GVVCTAEYEP TGDRVAIKKF FRPFQSTIHA KRTYRELKLL RTLQHDNVLE MIDVFTPDPD 

       130        140        150        160        170        180 
ASSLNNVYFV SVLMGSDLQN IMKIQRLTDE QIQLLIYQVL RGLKYIHSAG IIHRDLKPSN 

       190        200        210        220        230        240 
IAVNERCEVK VFLSFSQLSF LILSFFKILD FGLARAQDAE MTGYVATRWY RAPEIMLNWM 

       250        260        270        280        290        300 
HYTQTVDVWS VGCILAELVS GRPLFPGDDH IDQLTKIMSV VGTPKEEFWS KIQSEEARNY 

       310        320        330        340        350        360 
IKNRSPIIRQ DFVTLFPMAS PYALELLEMM LILDPDRRIS VSSALRHDYL REYSVPNDEP 

       370        380        390        400        410 
VAMDTVINSI VTIDPAEERA TTLSDWRELI WNEIRLFQNS ARRLSFVSCT DTEEEPMKI 

« Hide

Isoform b [UniParc].

Checksum: E9393A7F1AFAC2E2
Show »

FASTA39745,494

References

« Hide 'large scale' references
[1]"Isolation and characterization of pmk-(1-3): three p38 homologs in Caenorhabditis elegans."
Berman K., McKay J., Avery L., Cobb M.
Mol. Cell Biol. Res. Commun. 4:337-344(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION, ENZYME REGULATION.
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FO080126 Genomic DNA. Translation: CCD61402.1.
FO080126 Genomic DNA. Translation: CCD61403.1.
RefSeqNP_741457.1. NM_171392.4. [Q8MXI4-1]
NP_741458.2. NM_171913.4. [Q8MXI4-2]
UniGeneCel.7053.

3D structure databases

ProteinModelPortalQ8MXI4.
SMRQ8MXI4. Positions 33-397.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid42724. 2 interactions.
DIPDIP-24927N.
IntActQ8MXI4. 1 interaction.
MINTMINT-1042906.

Proteomic databases

PaxDbQ8MXI4.
PRIDEQ8MXI4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaF42G8.3a; F42G8.3a; WBGene00004056. [Q8MXI4-1]
GeneID177611.
KEGGcel:CELE_F42G8.3.
UCSCF42G8.3a. c. elegans. [Q8MXI4-1]

Organism-specific databases

CTD177611.
WormBaseF42G8.3a; CE49565; WBGene00004056; pmk-2.
F42G8.3b; CE34862; WBGene00004056; pmk-2.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00550000074271.
HOGENOMHOG000233024.
InParanoidQ8MXI4.
KOK04441.
OMAIAVNERC.
PhylomeDBQ8MXI4.

Enzyme and pathway databases

SignaLinkQ8MXI4.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01773. P38MAPKINASE.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio897584.

Entry information

Entry namePMK2_CAEEL
AccessionPrimary (citable) accession number: Q8MXI4
Secondary accession number(s): Q8MXI3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: October 1, 2002
Last modified: June 11, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase