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Q8MXI4

- PMK2_CAEEL

UniProt

Q8MXI4 - PMK2_CAEEL

Protein

Mitogen-activated protein kinase pmk-2

Gene

pmk-2

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.1 Publication

    Cofactori

    Magnesium.1 Publication

    Enzyme regulationi

    Activated by phosphorylation on threonine and tyrosine. Inhibited by pyridinyl-imidazole related compounds.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei78 – 781ATPPROSITE-ProRule annotation
    Active sitei210 – 2101Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi55 – 639ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB

    GO - Biological processi

    1. intracellular signal transduction Source: UniProtKB
    2. MAPK cascade Source: GOC
    3. response to osmotic stress Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SignaLinkiQ8MXI4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase pmk-2 (EC:2.7.11.24)
    Alternative name(s):
    Stress-activated protein kinase pmk-2
    p38 MAP kinase 2
    Gene namesi
    Name:pmk-2
    ORF Names:F42G8.3
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome IV

    Organism-specific databases

    WormBaseiF42G8.3a; CE49565; WBGene00004056; pmk-2.
    F42G8.3b; CE34862; WBGene00004056; pmk-2.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 419419Mitogen-activated protein kinase pmk-2PRO_0000186304Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei222 – 2221PhosphothreonineBy similarity
    Modified residuei224 – 2241PhosphotyrosineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-222 and Tyr-224, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8MXI4.
    PRIDEiQ8MXI4.

    Interactioni

    Protein-protein interaction databases

    BioGridi42724. 2 interactions.
    DIPiDIP-24927N.
    IntActiQ8MXI4. 1 interaction.
    MINTiMINT-1042906.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8MXI4.
    SMRiQ8MXI4. Positions 33-397.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini49 – 350302Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi222 – 2243TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00550000074271.
    HOGENOMiHOG000233024.
    InParanoidiQ8MXI4.
    KOiK04441.
    OMAiIAVNERC.
    PhylomeDBiQ8MXI4.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008352. MAPK_p38.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01773. P38MAPKINASE.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform aCurated (identifier: Q8MXI4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGMSATMGDS ASIPGVFFAD FGPAPPEITP EGYHEVELNK TKWVLPQWYN    50
    SLKPLGEGAY GVVCTAEYEP TGDRVAIKKF FRPFQSTIHA KRTYRELKLL 100
    RTLQHDNVLE MIDVFTPDPD ASSLNNVYFV SVLMGSDLQN IMKIQRLTDE 150
    QIQLLIYQVL RGLKYIHSAG IIHRDLKPSN IAVNERCEVK VFLSFSQLSF 200
    LILSFFKILD FGLARAQDAE MTGYVATRWY RAPEIMLNWM HYTQTVDVWS 250
    VGCILAELVS GRPLFPGDDH IDQLTKIMSV VGTPKEEFWS KIQSEEARNY 300
    IKNRSPIIRQ DFVTLFPMAS PYALELLEMM LILDPDRRIS VSSALRHDYL 350
    REYSVPNDEP VAMDTVINSI VTIDPAEERA TTLSDWRELI WNEIRLFQNS 400
    ARRLSFVSCT DTEEEPMKI 419
    Length:419
    Mass (Da):48,026
    Last modified:October 1, 2002 - v1
    Checksum:i621C52BFAB7EF37B
    GO
    Isoform bCurated (identifier: Q8MXI4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         190-206: Missing.
         366-370: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:397
    Mass (Da):45,494
    Checksum:iE9393A7F1AFAC2E2
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei190 – 20617Missing in isoform b. CuratedVSP_009272Add
    BLAST
    Alternative sequencei366 – 3705Missing in isoform b. CuratedVSP_050273

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO080126 Genomic DNA. Translation: CCD61402.1.
    FO080126 Genomic DNA. Translation: CCD61403.1.
    RefSeqiNP_741457.1. NM_171392.4. [Q8MXI4-1]
    NP_741458.2. NM_171913.4. [Q8MXI4-2]
    UniGeneiCel.7053.

    Genome annotation databases

    EnsemblMetazoaiF42G8.3a; F42G8.3a; WBGene00004056. [Q8MXI4-1]
    GeneIDi177611.
    KEGGicel:CELE_F42G8.3.
    UCSCiF42G8.3a. c. elegans. [Q8MXI4-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO080126 Genomic DNA. Translation: CCD61402.1 .
    FO080126 Genomic DNA. Translation: CCD61403.1 .
    RefSeqi NP_741457.1. NM_171392.4. [Q8MXI4-1 ]
    NP_741458.2. NM_171913.4. [Q8MXI4-2 ]
    UniGenei Cel.7053.

    3D structure databases

    ProteinModelPortali Q8MXI4.
    SMRi Q8MXI4. Positions 33-397.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 42724. 2 interactions.
    DIPi DIP-24927N.
    IntActi Q8MXI4. 1 interaction.
    MINTi MINT-1042906.

    Proteomic databases

    PaxDbi Q8MXI4.
    PRIDEi Q8MXI4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai F42G8.3a ; F42G8.3a ; WBGene00004056 . [Q8MXI4-1 ]
    GeneIDi 177611.
    KEGGi cel:CELE_F42G8.3.
    UCSCi F42G8.3a. c. elegans. [Q8MXI4-1 ]

    Organism-specific databases

    CTDi 177611.
    WormBasei F42G8.3a ; CE49565 ; WBGene00004056 ; pmk-2.
    F42G8.3b ; CE34862 ; WBGene00004056 ; pmk-2.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00550000074271.
    HOGENOMi HOG000233024.
    InParanoidi Q8MXI4.
    KOi K04441.
    OMAi IAVNERC.
    PhylomeDBi Q8MXI4.

    Enzyme and pathway databases

    SignaLinki Q8MXI4.

    Miscellaneous databases

    NextBioi 897584.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008352. MAPK_p38.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01773. P38MAPKINASE.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of pmk-(1-3): three p38 homologs in Caenorhabditis elegans."
      Berman K., McKay J., Avery L., Cobb M.
      Mol. Cell Biol. Res. Commun. 4:337-344(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION, ENZYME REGULATION.
      Strain: Bristol N2.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiPMK2_CAEEL
    AccessioniPrimary (citable) accession number: Q8MXI4
    Secondary accession number(s): Q8MXI3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3