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Q8MXI4

- PMK2_CAEEL

UniProt

Q8MXI4 - PMK2_CAEEL

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Protein

Mitogen-activated protein kinase pmk-2

Gene

pmk-2

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Magnesium.1 Publication

Enzyme regulationi

Activated by phosphorylation on threonine and tyrosine. Inhibited by pyridinyl-imidazole related compounds.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei78 – 781ATPPROSITE-ProRule annotation
Active sitei210 – 2101Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi55 – 639ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB

GO - Biological processi

  1. intracellular signal transduction Source: UniProtKB
  2. MAPK cascade Source: GOC
  3. response to osmotic stress Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ8MXI4.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase pmk-2 (EC:2.7.11.24)
Alternative name(s):
Stress-activated protein kinase pmk-2
p38 MAP kinase 2
Gene namesi
Name:pmk-2
ORF Names:F42G8.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome IV

Organism-specific databases

WormBaseiF42G8.3a; CE49565; WBGene00004056; pmk-2.
F42G8.3b; CE34862; WBGene00004056; pmk-2.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 419419Mitogen-activated protein kinase pmk-2PRO_0000186304Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei222 – 2221PhosphothreonineBy similarity
Modified residuei224 – 2241PhosphotyrosineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-222 and Tyr-224, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8MXI4.
PRIDEiQ8MXI4.

Interactioni

Protein-protein interaction databases

BioGridi42724. 2 interactions.
DIPiDIP-24927N.
IntActiQ8MXI4. 1 interaction.
MINTiMINT-1042906.

Structurei

3D structure databases

ProteinModelPortaliQ8MXI4.
SMRiQ8MXI4. Positions 8-397.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini49 – 350302Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi222 – 2243TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074271.
HOGENOMiHOG000233024.
InParanoidiQ8MXI4.
KOiK04441.
OMAiIAVNERC.
PhylomeDBiQ8MXI4.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform aCurated (identifier: Q8MXI4) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGMSATMGDS ASIPGVFFAD FGPAPPEITP EGYHEVELNK TKWVLPQWYN
60 70 80 90 100
SLKPLGEGAY GVVCTAEYEP TGDRVAIKKF FRPFQSTIHA KRTYRELKLL
110 120 130 140 150
RTLQHDNVLE MIDVFTPDPD ASSLNNVYFV SVLMGSDLQN IMKIQRLTDE
160 170 180 190 200
QIQLLIYQVL RGLKYIHSAG IIHRDLKPSN IAVNERCEVK VFLSFSQLSF
210 220 230 240 250
LILSFFKILD FGLARAQDAE MTGYVATRWY RAPEIMLNWM HYTQTVDVWS
260 270 280 290 300
VGCILAELVS GRPLFPGDDH IDQLTKIMSV VGTPKEEFWS KIQSEEARNY
310 320 330 340 350
IKNRSPIIRQ DFVTLFPMAS PYALELLEMM LILDPDRRIS VSSALRHDYL
360 370 380 390 400
REYSVPNDEP VAMDTVINSI VTIDPAEERA TTLSDWRELI WNEIRLFQNS
410
ARRLSFVSCT DTEEEPMKI
Length:419
Mass (Da):48,026
Last modified:October 1, 2002 - v1
Checksum:i621C52BFAB7EF37B
GO
Isoform bCurated (identifier: Q8MXI4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     190-206: Missing.
     366-370: Missing.

Note: No experimental confirmation available.

Show »
Length:397
Mass (Da):45,494
Checksum:iE9393A7F1AFAC2E2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei190 – 20617Missing in isoform b. CuratedVSP_009272Add
BLAST
Alternative sequencei366 – 3705Missing in isoform b. CuratedVSP_050273

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FO080126 Genomic DNA. Translation: CCD61402.1.
FO080126 Genomic DNA. Translation: CCD61403.1.
RefSeqiNP_741457.1. NM_171392.4. [Q8MXI4-1]
NP_741458.2. NM_171913.4. [Q8MXI4-2]
UniGeneiCel.7053.

Genome annotation databases

EnsemblMetazoaiF42G8.3a; F42G8.3a; WBGene00004056. [Q8MXI4-1]
GeneIDi177611.
KEGGicel:CELE_F42G8.3.
UCSCiF42G8.3a. c. elegans. [Q8MXI4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FO080126 Genomic DNA. Translation: CCD61402.1 .
FO080126 Genomic DNA. Translation: CCD61403.1 .
RefSeqi NP_741457.1. NM_171392.4. [Q8MXI4-1 ]
NP_741458.2. NM_171913.4. [Q8MXI4-2 ]
UniGenei Cel.7053.

3D structure databases

ProteinModelPortali Q8MXI4.
SMRi Q8MXI4. Positions 8-397.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 42724. 2 interactions.
DIPi DIP-24927N.
IntActi Q8MXI4. 1 interaction.
MINTi MINT-1042906.

Proteomic databases

PaxDbi Q8MXI4.
PRIDEi Q8MXI4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai F42G8.3a ; F42G8.3a ; WBGene00004056 . [Q8MXI4-1 ]
GeneIDi 177611.
KEGGi cel:CELE_F42G8.3.
UCSCi F42G8.3a. c. elegans. [Q8MXI4-1 ]

Organism-specific databases

CTDi 177611.
WormBasei F42G8.3a ; CE49565 ; WBGene00004056 ; pmk-2.
F42G8.3b ; CE34862 ; WBGene00004056 ; pmk-2.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074271.
HOGENOMi HOG000233024.
InParanoidi Q8MXI4.
KOi K04441.
OMAi IAVNERC.
PhylomeDBi Q8MXI4.

Enzyme and pathway databases

SignaLinki Q8MXI4.

Miscellaneous databases

NextBioi 897584.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01773. P38MAPKINASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of pmk-(1-3): three p38 homologs in Caenorhabditis elegans."
    Berman K., McKay J., Avery L., Cobb M.
    Mol. Cell Biol. Res. Commun. 4:337-344(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION, ENZYME REGULATION.
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.

Entry informationi

Entry nameiPMK2_CAEEL
AccessioniPrimary (citable) accession number: Q8MXI4
Secondary accession number(s): Q8MXI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3