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Protein

Mitogen-activated protein kinase pmk-2

Gene

pmk-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+1 Publication

Enzyme regulationi

Activated by phosphorylation on threonine and tyrosine. Inhibited by pyridinyl-imidazole related compounds.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78ATPPROSITE-ProRule annotation1
Active sitei210Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi55 – 63ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: UniProtKB

GO - Biological processi

  • intracellular signal transduction Source: UniProtKB
  • regulation of gene expression Source: GO_Central
  • response to osmotic stress Source: UniProtKB

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-168638 NOD1/2 Signaling Pathway
R-CEL-171007 p38MAPK events
R-CEL-198753 ERK/MAPK targets
R-CEL-2559580 Oxidative Stress Induced Senescence
R-CEL-375170 CDO in myogenesis
R-CEL-418592 ADP signalling through P2Y purinoceptor 1
R-CEL-432142 Platelet sensitization by LDL
R-CEL-4420097 VEGFA-VEGFR2 Pathway
R-CEL-450302 activated TAK1 mediates p38 MAPK activation
R-CEL-6798695 Neutrophil degranulation
SignaLinkiQ8MXI4

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase pmk-2 (EC:2.7.11.24)
Alternative name(s):
Stress-activated protein kinase pmk-2
p38 MAP kinase 2
Gene namesi
Name:pmk-2
ORF Names:F42G8.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiF42G8.3a ; CE49565 ; WBGene00004056 ; pmk-2
F42G8.3b ; CE34862 ; WBGene00004056 ; pmk-2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863041 – 419Mitogen-activated protein kinase pmk-2Add BLAST419

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei222PhosphothreonineBy similarity1
Modified residuei224PhosphotyrosineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-222 and Tyr-224, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8MXI4
PeptideAtlasiQ8MXI4
PRIDEiQ8MXI4

Expressioni

Gene expression databases

BgeeiWBGene00004056
ExpressionAtlasiQ8MXI4 baseline and differential

Interactioni

Protein-protein interaction databases

BioGridi42724, 2 interactors
DIPiDIP-24927N
IntActiQ8MXI4, 1 interactor
STRINGi6239.F42G8.3a.2

Structurei

3D structure databases

ProteinModelPortaliQ8MXI4
SMRiQ8MXI4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 350Protein kinasePROSITE-ProRule annotationAdd BLAST302

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi222 – 224TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660 Eukaryota
ENOG410XNY0 LUCA
HOGENOMiHOG000233024
InParanoidiQ8MXI4
KOiK04441
OrthoDBiEOG091G08QL
PhylomeDBiQ8MXI4

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR008352 MAPK_p38-like
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PRINTSiPR01773 P38MAPKINASE
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform aCurated (identifier: Q8MXI4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGMSATMGDS ASIPGVFFAD FGPAPPEITP EGYHEVELNK TKWVLPQWYN
60 70 80 90 100
SLKPLGEGAY GVVCTAEYEP TGDRVAIKKF FRPFQSTIHA KRTYRELKLL
110 120 130 140 150
RTLQHDNVLE MIDVFTPDPD ASSLNNVYFV SVLMGSDLQN IMKIQRLTDE
160 170 180 190 200
QIQLLIYQVL RGLKYIHSAG IIHRDLKPSN IAVNERCEVK VFLSFSQLSF
210 220 230 240 250
LILSFFKILD FGLARAQDAE MTGYVATRWY RAPEIMLNWM HYTQTVDVWS
260 270 280 290 300
VGCILAELVS GRPLFPGDDH IDQLTKIMSV VGTPKEEFWS KIQSEEARNY
310 320 330 340 350
IKNRSPIIRQ DFVTLFPMAS PYALELLEMM LILDPDRRIS VSSALRHDYL
360 370 380 390 400
REYSVPNDEP VAMDTVINSI VTIDPAEERA TTLSDWRELI WNEIRLFQNS
410
ARRLSFVSCT DTEEEPMKI
Length:419
Mass (Da):48,026
Last modified:October 1, 2002 - v1
Checksum:i621C52BFAB7EF37B
GO
Isoform bCurated (identifier: Q8MXI4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     190-206: Missing.
     366-370: Missing.

Note: No experimental confirmation available.
Show »
Length:397
Mass (Da):45,494
Checksum:iE9393A7F1AFAC2E2
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009272190 – 206Missing in isoform b. CuratedAdd BLAST17
Alternative sequenceiVSP_050273366 – 370Missing in isoform b. Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080126 Genomic DNA Translation: CCD61402.1
FO080126 Genomic DNA Translation: CCD61403.1
RefSeqiNP_741457.2, NM_171392.5
NP_741458.2, NM_171913.4 [Q8MXI4-2]
UniGeneiCel.7053

Genome annotation databases

GeneIDi177611
KEGGicel:CELE_F42G8.3
UCSCiF42G8.3a c. elegans [Q8MXI4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPMK2_CAEEL
AccessioniPrimary (citable) accession number: Q8MXI4
Secondary accession number(s): Q8MXI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: October 1, 2002
Last modified: May 23, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

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