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Protein

Guanine nucleotide-binding protein-like 3 homolog

Gene

Ns1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in regulating cellular proliferation.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi189 – 192GTPSequence analysis4
Nucleotide bindingi274 – 281GTPSequence analysis8
Nucleotide bindingi318 – 321GTPSequence analysis4

GO - Molecular functioni

  • ATPase activity Source: FlyBase
  • GTPase activity Source: FlyBase
  • GTP binding Source: UniProtKB

GO - Biological processi

  • cellular response to starvation Source: FlyBase
  • mitotic spindle assembly Source: FlyBase
  • positive regulation of multicellular organism growth Source: FlyBase
  • regulation of cell proliferation Source: UniProtKB
  • regulation of insulin receptor signaling pathway Source: FlyBase
  • regulation of multicellular organism growth Source: FlyBase
  • ribosomal large subunit biogenesis Source: FlyBase

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein-like 3 homolog
Alternative name(s):
Nucleostemin 1
Nucleostemin homolog
Gene namesi
Name:Ns1
ORF Names:CG3983
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0038473. Ns1.

Subcellular locationi

GO - Cellular componenti

  • granular component Source: FlyBase
  • nucleolus Source: UniProtKB

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Impaired embryonic development resulting in a profoundly reduced rate of larval hatching.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001224511 – 581Guanine nucleotide-binding protein-like 3 homologAdd BLAST581

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei99Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8MT06.
PRIDEiQ8MT06.

PTM databases

iPTMnetiQ8MT06.

Expressioni

Gene expression databases

BgeeiFBgn0038473.
GenevisibleiQ8MT06. DM.

Interactioni

Protein-protein interaction databases

BioGridi67100. 12 interactors.
IntActiQ8MT06. 2 interactors.
STRINGi7227.FBpp0082867.

Structurei

3D structure databases

ProteinModelPortaliQ8MT06.
SMRiQ8MT06.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini141 – 325CP-type GPROSITE-ProRule annotationAdd BLAST185

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili64 – 108Sequence analysisAdd BLAST45

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi4 – 49Lys-richAdd BLAST46

Domaini

In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.

Sequence similaritiesi

Belongs to the TRAFAC class YlqF/YawG GTPase family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2484. Eukaryota.
COG1161. LUCA.
InParanoidiQ8MT06.
KOiK14538.
OMAiWIKYFRR.
OrthoDBiEOG091G08IZ.
PhylomeDBiQ8MT06.

Family and domain databases

Gene3Di1.10.1580.10. 1 hit.
InterProiView protein in InterPro
IPR030378. G_CP_dom.
IPR014813. Gnl3_N_dom.
IPR023179. GTP-bd_ortho_bundle.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF08701. GN3L_Grn1. 1 hit.
PF01926. MMR_HSR1. 1 hit.
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS51721. G_CP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8MT06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKRLKTKK SKRLTGRLKH KIEKKVRDHN KKERRAAKKN PKKGSKKQKL
60 70 80 90 100
IQIPNICPFK DDILKEVEEA KQRQEAERLA RREAFKAERE QNKFKTLESM
110 120 130 140 150
VEDADMRSTV HGIMHENDAQ DQDEKKYKNA VTKEQSLKQY FKEFRKVIEN
160 170 180 190 200
ADVVLEVVDA RDPLGTRCNE VERAVRGAPG NKRLVLVLNK ADLVPRENLN
210 220 230 240 250
NWIKYFRRSG PVTAFKASTQ DQANRLGRRK LREMKTEKAM QGSVCIGAEL
260 270 280 290 300
LMSMLGNYCR NKGIKTSIRV GVVGIPNVGK SSIINSLTRG RSCMVGSTPG
310 320 330 340 350
VTKSMQEVEL DSKIKLIDCP GIVFTSGGEN SHAVLKNAQR VGDVKDPFTI
360 370 380 390 400
AESVLKRASK EYFCTMYDIT NYDTFEEFFA KKAARMGKFL KKGVPDVVAA
410 420 430 440 450
ARSVLNDWNT GKIKYCTQPP EVQEGQSVHI SASIVHSEAR EFDVENFESM
460 470 480 490 500
ETEILEHCAV KTDDIMEITS TGPLEIRQPR EEAEPADKIT ASLVIDEKEK
510 520 530 540 550
PAKGRKRKLD EEKEKVDPSL LLEENQSLNK GIKQMQKLKK KQNVRNEKKI
560 570 580
SKITDVLDSF SLGPSSSKAE KYDFDEDYVI E
Length:581
Mass (Da):65,985
Last modified:April 12, 2005 - v2
Checksum:i77065E7A0430EEB7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94F → I in AAM49824 (PubMed:12537569).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF55384.3.
AY118455 mRNA. Translation: AAM49824.1.
BT050580 mRNA. Translation: ACJ23464.1.
RefSeqiNP_732199.2. NM_169742.3.
UniGeneiDm.11627.

Genome annotation databases

EnsemblMetazoaiFBtr0083426; FBpp0082867; FBgn0038473.
GeneIDi42060.
KEGGidme:Dmel_CG3983.
UCSCiCG3983-RA. d. melanogaster.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGNL3_DROME
AccessioniPrimary (citable) accession number: Q8MT06
Secondary accession number(s): A4V308, B6IDZ0, Q9VEN7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: April 12, 2005
Last modified: July 5, 2017
This is version 108 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families