Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein alan shepard

Gene

shep

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in the perception of gravity.1 Publication

GO - Molecular functioni

  • mRNA binding Source: FlyBase
  • nucleotide binding Source: InterPro

GO - Biological processi

  • adult locomotory behavior Source: FlyBase
  • female courtship behavior Source: FlyBase
  • gravitaxis Source: FlyBase
  • metamorphosis Source: FlyBase
  • neuron remodeling Source: FlyBase
  • response to endoplasmic reticulum stress Source: FlyBase
  • response to gravity Source: UniProtKB
  • sleep Source: FlyBase
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein alan shepard
Gene namesi
Name:shepImported
ORF Names:CG32423
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0052423. shep.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 590590Protein alan shepardPRO_0000379499Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei5 – 51Phosphotyrosine1 Publication
Modified residuei125 – 1251Phosphotyrosine1 Publication
Modified residuei142 – 1421Phosphotyrosine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8MSV2.
PRIDEiQ8MSV2.

PTM databases

iPTMnetiQ8MSV2.

Expressioni

Gene expression databases

BgeeiQ8MSV2.
GenevisibleiQ8MSV2. DM.

Interactioni

Protein-protein interaction databases

BioGridi64073. 1 interaction.
IntActiQ8MSV2. 27 interactions.
MINTiMINT-339377.
STRINGi7227.FBpp0301014.

Structurei

3D structure databases

ProteinModelPortaliQ8MSV2.
SMRiQ8MSV2. Positions 241-399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini243 – 31674RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini322 – 40180RRM 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi62 – 15594Ala-richSequence analysisAdd
BLAST

Sequence similaritiesi

Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4733. Eukaryota.
ENOG410XRB5. LUCA.
GeneTreeiENSGT00760000118913.
InParanoidiQ8MSV2.
OMAiNTNTNMG.
OrthoDBiEOG71G9V3.
PhylomeDBiQ8MSV2.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR002343. Hud_Sxl_RNA.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
PRINTSiPR00961. HUDSXLRNA.
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform F (identifier: Q8MSV2-5) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHPRYSPAPP PQQQQQMGGP PHQQQGGGGG GGVSMRGPSN AQQLPPQIPR
60 70 80 90 100
SQNYSNGSSS SAAAAPLTSR SAFPGAPLTA SAVALKGALP QRPPAMTSPA
110 120 130 140 150
AAAAGAALAA GAPYRGAASW TPQGYAPAAA AAAAAVAQQA AYRYTAPLPQ
160 170 180 190 200
PAYAAYTPHT ATTPATTTVS FLSQPVDYYW YGQRVPTAAS PSNTNSSSSS
210 220 230 240 250
NTGSQSGTLS TSLSNTTNTN TNMGPNGTVQ NQNQQGGEQL SKTNLYIRGL
260 270 280 290 300
QQGTTDKDLV NMCAQYGTII STKAILDKTT NKCKGYGFVD FEQPAFAECA
310 320 330 340 350
VKGLQGKGVQ AQMAKQQEQD PTNLYIANLP PHFKETDLEA MLSKYGQVVS
360 370 380 390 400
TRILRDQQMN SKGVGFARME SREKCEQIIQ MFNGNTIPGA KDPLLVKFAD
410 420 430 440 450
GGPKKKNLFK TPDPNARAWR DVSAEGIPVA YDPTMQQNGV SVNVGTPIGV
460 470 480 490 500
PYSRFSAPQV GGYPVAGSQW IPGYMMTQVD DQTSYSPQYM QMAAAPPLGV
510 520 530 540 550
TSYKPEAVNQ VQPRGISMMV SGDTGVPYGT MMPQLATLQI GNSYISPTYP
560 570 580 590
YYAPPPTIIP TMPMTDSEQA STAASPDEAY TQYPHQAAPK
Note: No experimenal confirmation available.
Length:590
Mass (Da):62,175
Last modified:April 16, 2014 - v2
Checksum:i7700D1E378A5255A
GO
Isoform A1 Publication (identifier: Q8MSV2-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     169-180: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:578
Mass (Da):60,689
Checksum:iA834D141737A0A89
GO
Isoform B1 Publication (identifier: Q8MSV2-2) [UniParc]FASTAAdd to basket

Also known as: D1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-95: Missing.
     169-180: Missing.
     284-285: Missing.
     315-315: K → KVGIWVLHRPAI
     543-543: S → SNFSPSLQ

Note: No experimental confirmation available.Curated
Show »
Length:499
Mass (Da):53,021
Checksum:i741195A5D8E51552
GO
Isoform E1 Publication (identifier: Q8MSV2-4) [UniParc]FASTAAdd to basket

Also known as: G

The sequence of this isoform differs from the canonical sequence as follows:
     1-222: Missing.
     315-315: K → KVGIWVLHRPAI

Note: No experimental confirmation available.Curated
Show »
Length:379
Mass (Da):41,266
Checksum:i741C06806CB3B168
GO
Isoform I (identifier: Q8MSV2-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-222: Missing.
     316-317: QQ → VGIWVLHRPAI

Note: No experimental confirmation available.
Show »
Length:377
Mass (Da):41,010
Checksum:iB82E7FDFC03FE5CE
GO
Isoform H (identifier: Q8MSV2-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-222: Missing.
     316-317: QQ → VGIWVLHRPAI
     483-488: Missing.

Note: No experimental confirmation available.
Show »
Length:371
Mass (Da):40,346
Checksum:iEA8DEB33523808DE
GO

Sequence cautioni

The sequence AAL28502.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAL28543.1 differs from that shown. Reason: Frameshift at position 420. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 222222Missing in isoform E, isoform H and isoform I. 1 PublicationVSP_054212Add
BLAST
Alternative sequencei1 – 9595Missing in isoform B. 2 PublicationsVSP_054213Add
BLAST
Alternative sequencei169 – 18012Missing in isoform A and isoform B. 1 PublicationVSP_054214Add
BLAST
Alternative sequencei284 – 2852Missing in isoform B. 1 PublicationVSP_054215
Alternative sequencei315 – 3151K → KVGIWVLHRPAI in isoform B and isoform E. 1 PublicationVSP_054216
Alternative sequencei316 – 3172QQ → VGIWVLHRPAI in isoform I and isoform H. CuratedVSP_054217
Alternative sequencei483 – 4886Missing in isoform H. CuratedVSP_054218
Alternative sequencei543 – 5431S → SNFSPSLQ in isoform B. 1 PublicationVSP_054219

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF50788.5.
AE014296 Genomic DNA. Translation: AAF50790.2.
AE014296 Genomic DNA. Translation: AAF50792.4.
AE014296 Genomic DNA. Translation: AAN12240.1.
AE014296 Genomic DNA. Translation: ACZ94622.1.
AE014296 Genomic DNA. Translation: AFH04307.1.
AE014296 Genomic DNA. Translation: AGB94133.1.
AE014296 Genomic DNA. Translation: AGB94134.1.
AY060954 mRNA. Translation: AAL28502.1. Different initiation.
AY060995 mRNA. Translation: AAL28543.1. Frameshift.
AY094948 mRNA. Translation: AAM11301.1.
AY118564 mRNA. Translation: AAM49933.1.
RefSeqiNP_001163350.1. NM_001169879.2. [Q8MSV2-4]
NP_001246636.1. NM_001259707.1. [Q8MSV2-5]
NP_001261438.1. NM_001274509.1. [Q8MSV2-4]
NP_001261439.1. NM_001274510.1. [Q8MSV2-7]
NP_729054.3. NM_168111.4. [Q8MSV2-1]
NP_729055.1. NM_168112.4. [Q8MSV2-2]
NP_729056.1. NM_168113.3. [Q8MSV2-2]
NP_729057.3. NM_168114.5. [Q8MSV2-6]
UniGeneiDm.20861.

Genome annotation databases

EnsemblMetazoaiFBtr0308859; FBpp0301014; FBgn0052423. [Q8MSV2-5]
GeneIDi38605.
KEGGidme:Dmel_CG32423.
UCSCiCG32423-RA. d. melanogaster. [Q8MSV2-5]
CG32423-RB. d. melanogaster.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF50788.5.
AE014296 Genomic DNA. Translation: AAF50790.2.
AE014296 Genomic DNA. Translation: AAF50792.4.
AE014296 Genomic DNA. Translation: AAN12240.1.
AE014296 Genomic DNA. Translation: ACZ94622.1.
AE014296 Genomic DNA. Translation: AFH04307.1.
AE014296 Genomic DNA. Translation: AGB94133.1.
AE014296 Genomic DNA. Translation: AGB94134.1.
AY060954 mRNA. Translation: AAL28502.1. Different initiation.
AY060995 mRNA. Translation: AAL28543.1. Frameshift.
AY094948 mRNA. Translation: AAM11301.1.
AY118564 mRNA. Translation: AAM49933.1.
RefSeqiNP_001163350.1. NM_001169879.2. [Q8MSV2-4]
NP_001246636.1. NM_001259707.1. [Q8MSV2-5]
NP_001261438.1. NM_001274509.1. [Q8MSV2-4]
NP_001261439.1. NM_001274510.1. [Q8MSV2-7]
NP_729054.3. NM_168111.4. [Q8MSV2-1]
NP_729055.1. NM_168112.4. [Q8MSV2-2]
NP_729056.1. NM_168113.3. [Q8MSV2-2]
NP_729057.3. NM_168114.5. [Q8MSV2-6]
UniGeneiDm.20861.

3D structure databases

ProteinModelPortaliQ8MSV2.
SMRiQ8MSV2. Positions 241-399.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi64073. 1 interaction.
IntActiQ8MSV2. 27 interactions.
MINTiMINT-339377.
STRINGi7227.FBpp0301014.

PTM databases

iPTMnetiQ8MSV2.

Proteomic databases

PaxDbiQ8MSV2.
PRIDEiQ8MSV2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0308859; FBpp0301014; FBgn0052423. [Q8MSV2-5]
GeneIDi38605.
KEGGidme:Dmel_CG32423.
UCSCiCG32423-RA. d. melanogaster. [Q8MSV2-5]
CG32423-RB. d. melanogaster.

Organism-specific databases

CTDi38605.
FlyBaseiFBgn0052423. shep.

Phylogenomic databases

eggNOGiKOG4733. Eukaryota.
ENOG410XRB5. LUCA.
GeneTreeiENSGT00760000118913.
InParanoidiQ8MSV2.
OMAiNTNTNMG.
OrthoDBiEOG71G9V3.
PhylomeDBiQ8MSV2.

Miscellaneous databases

ChiTaRSishep. fly.
GenomeRNAii38605.
PROiQ8MSV2.

Gene expression databases

BgeeiQ8MSV2.
GenevisibleiQ8MSV2. DM.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR002343. Hud_Sxl_RNA.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
PRINTSiPR00961. HUDSXLRNA.
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A; B AND E), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 398-590 (ISOFORMS A/E/F/I).
    Strain: BerkeleyImported.
    Tissue: Embryo1 Publication, Head1 Publication and Ovary1 Publication.
  4. "Gravitaxis in Drosophila melanogaster: a forward genetic screen."
    Armstrong J.D., Texada M.J., Munjaal R., Baker D.A., Beckingham K.M.
    Genes Brain Behav. 5:222-239(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, FUNCTION.
  5. "An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells."
    Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A.
    Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-125 AND TYR-142, IDENTIFICATION BY MASS SPECTROMETRY.
  6. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-5, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo1 Publication.

Entry informationi

Entry nameiSHEP_DROME
AccessioniPrimary (citable) accession number: Q8MSV2
Secondary accession number(s): E1JID7
, M9ND50, M9PEP6, Q7KU60, Q86BS4, Q8SWY6, Q95S20, Q95S52, Q9VRK3, Q9VRK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: April 16, 2014
Last modified: July 6, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

Named after Alan Bartlett Shepard, Jr. who was the second person and the first American in space and the fifth person to walk on the moon.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.