Reviewed, UniProtKB/Swiss-Prot Q8MSS1 (LVA_DROME)

Names and origin

Protein names

Recommended name:
    Protein lava lamp

Gene names

Name:	lva
ORF Names:	CG6450

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	2779 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Lva and spectrin may form a Golgi-based scaffold that mediates interaction of Golgi bodies with microtubules and facilitates Golgi-derived membrane secretion required for the formation of furrows during cellularization. Ref.4
Subunit structure	Interacts with CLIP-190 and spectrin separately. Ref.4
Subcellular location	Golgi apparatus. Note: Lva-alpha-spectrin and Lva-CLIP-190 complexes are found at the Golgi. Ref.4

Ontologies

Keywords
Cellular component	Golgi apparatus
Domain	Coiled coil
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellularization Ref.4 Inferred from mutant phenotype. Source: UniProtKB establishment of Golgi localization Inferred from mutant phenotype. Source: FlyBase negative regulation of dendrite morphogenesis Inferred from mutant phenotype. Source: FlyBase
Cellular component	Golgi apparatus Ref.4 Inferred from direct assay. Source: UniProtKB
Molecular function	microtubule binding Ref.4 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2779	2779	Protein lava lamp		PRO_0000084526
Regions
	Coiled coil	52 – 85	34	Potential
	Coiled coil	141 – 175	35	Potential
	Coiled coil	220 – 607	388	Potential
	Coiled coil	659 – 716	58	Potential
	Coiled coil	751 – 1733	983	Potential
	Coiled coil	1785 – 1863	79	Potential
	Coiled coil	1941 – 2433	493	Potential
	Coiled coil	2504 – 2544	41	Potential
	Coiled coil	2600 – 2641	42	Potential
Amino acid modifications
	Modified residue	34	1	Phosphoserine Ref.5
	Modified residue	35	1	Phosphoserine Ref.5
	Modified residue	95	1	Phosphoserine Ref.5
	Modified residue	98	1	Phosphoserine Ref.5
	Modified residue	122	1	Phosphoserine Ref.5
	Modified residue	133	1	Phosphoserine Ref.5
	Modified residue	186	1	Phosphoserine Ref.5
	Modified residue	352	1	Phosphoserine Ref.5
	Modified residue	354	1	Phosphoserine Ref.5
Experimental info
	Sequence conflict	1211	1	E → K in AAM50007. Ref.3
	Sequence conflict	1424	1	A → S in AAM50007. Ref.3
	Sequence conflict	1506	1	E → D in AAM50007. Ref.3
	Sequence conflict	2034	1	A → V in AAM50007. Ref.3
	Sequence conflict	2069	1	Q → H in AAM50007. Ref.3
	Sequence conflict	2133	1	G → E in AAM50007. Ref.3
	Sequence conflict	2154	1	Q → E in AAM50007. Ref.3
	Sequence conflict	2160	1	E → V in AAM50007. Ref.3
	Sequence conflict	2200	1	L → P in AAM50007. Ref.3
	Sequence conflict	2217	1	A → T in AAM50007. Ref.3
	Sequence conflict	2271	1	E → D in AAM50007. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8MSS1-1 [UniParc]. Last modified May 9, 2003. Version 2. Checksum: 1CB3965102018AEE	FASTA	2,779	315,900
	10 20 30 40 50 60 MAEDSGALES SYDFSIVQPD DHEYGEADIR LAGSSNDLSS LQNVSASTTR GTKGKGRLDS 70 80 90 100 110 120 LKENLYKQQE RLTALKERAL RKSQDERHKS SMSDSMESLK TLGQKLTVLK TRSGDSSTPL 130 140 150 160 170 180 VSPTKDSDPG DVSLLQTSGS EKLLMLTQRT EQNRALLEQR KRDLAKSLLS VKSNIGHQTT 190 200 210 220 230 240 AELGSSMTDL RHAASVSNPP VSRHRSALDL EAQGQEAVDE SRVKLLRSRM KLTELKQGRQ 250 260 270 280 290 300 EQELNELRTE LAKRAKLIER LELSGAELQR TLTQRNEELE QLRVVQAEED SLKVQENSRL 310 320 330 340 350 360 QGEVLVLRER LAELENVNDL LETTRCELQE ELTTARERQR NLELEQEQEK ASRSPQSEAA 370 380 390 400 410 420 HTDAQVSAEL AKQLQELTNQ LADLQATNEE LRQQVAAQAK LQVTDEIVSQ RLEELEATIA 430 440 450 460 470 480 AQLLELQEQK SAMAAQNEEL AEKTTELNVL NVNLRLLEEK LAQSSRSKPL FLEDHSEDSA 490 500 510 520 530 540 ASKQMQEDLQ QLKLKLDETN KANIKLKLKC KQAEKKLQKF QSQDGQQQLA SLLADNEELQ 550 560 570 580 590 600 QRIAVLEDEK GQWQLANMQE DDRQPEQSTE SNNPLQLETI RLLEEQKLEL QQALEALLSS 610 620 630 640 650 660 SSSAESIEIV ERHHLECLGQ RRPASEGDAQ EQKQVHPPGP SHVSELTQTE QTEEEDSSGE 670 680 690 700 710 720 TLSQLRERLE LFTQERGEVL DKLEQLSAEN LQLQARLEES SSSLQLLQRE REKDLISSTS 730 740 750 760 770 780 TSSNLSQELS SMQRSSEVVA TLDAGEGGPV LFEKCEKSLS KLNSELEAYR KANDRQAKFN 790 800 810 820 830 840 VSKKLAKEAK NCHTQLSELL HKVKEASTAV ETVTVVETVV AVTAPNGKAL AEYEQLNAQN 850 860 870 880 890 900 AELKAVISRL RQELDELRES YPETEAPLAI VGSDSQREDE ILQLQSQLED ARSLQAEQRQ 910 920 930 940 950 960 QIEEQVDQIK ELRQTEAEQL QLVARQSAEI TQLQLQSEQF DQLLNSKEMS HEKQLEQQTR 970 980 990 1000 1010 1020 IRRELEARAE SLEGELSILQ TLVAEQKQQL IESVSESEHA LNLKMLELQS AQEELRELRA 1030 1040 1050 1060 1070 1080 KEDPDQLREA LRVSKSLVAQ QVRELTSSQE TVDALNQQIQ EYQGLEHAHK EEQFKNRELR 1090 1100 1110 1120 1130 1140 EKLKKYALNL KKRTQDNADL EQKVQELTSQ LQEQQELVKQ KEEVEREPIV DNHRVEQLQQ 1150 1160 1170 1180 1190 1200 QVSKLNEDLK AKIHLNLENR DALRQLKQQI QEQEQLIQER DAELQDANLV SKELRRERQE 1210 1220 1230 1240 1250 1260 ADQEVFQLGQ ENSRLREEIS KLQEEIHNLG QRVNEEPTAV EDLRRQLEAK SKKFEKSKEL 1270 1280 1290 1300 1310 1320 IKLRNATIQS LQRELQQLQQ DQDSEVEHVR NARAAHEQLR LEKDAEITAL RQEILKLERS 1330 1340 1350 1360 1370 1380 RAAGEGDDTI TKTSHQLLES QSQQQAESLQ VAERELQQLR VQLTAAQEQH ALLAQQYASD 1390 1400 1410 1420 1430 1440 KANFEMTIAR LETLHEGIQA KLQEDASYIE SLEAQNTELQ ARSAALEEQA ASQANQQAAS 1450 1460 1470 1480 1490 1500 QDKVQILEQQ LKEQREQEEQ KRQQDQQLQE RFYELGQREQ AQSRQLELLT SEAEESRQQL 1510 1520 1530 1540 1550 1560 AGLRTEYESL LAKHSQLTAT AQAEREQMSS HSQEELAELR QQLDVKEADL HRQRQVYDAK 1570 1580 1590 1600 1610 1620 LAAKATELDE LECDLNSHVE RAAAETRELC QQLERSQELV AQRTEELQRL NEEFQEVERE 1630 1640 1650 1660 1670 1680 RSTLSREVTL LRLQHDSAEQ DVLELQELRM QAMQDKTEMD NLRTQIDALC ANHSQELQAL 1690 1700 1710 1720 1730 1740 QQRIAELDTL GQNQTDDQVY IETENKRLAE QLSELQAQLA RQQHQQQQQQ HHHPAVQSQQ 1750 1760 1770 1780 1790 1800 HPPPASLFFG GDALAAPSPF DEIAQPLRVS SLAASAPPPI SPPPTIEDLQ RNVSDLEKHA 1810 1820 1830 1840 1850 1860 QDLETKLLAR NQNLAEQEER RLQLEQRLSE VERLLSERTQ QLADIQTANE ERDRLAALEK 1870 1880 1890 1900 1910 1920 LIQPAAAPTL DMFFGGQAEE TVPDAVSHHL DLGLPQTEPV VEPLIQPKKA YLCQPKQEIQ 1930 1940 1950 1960 1970 1980 EQTAQTIDWG VDEDPWASAA NEAPQTDVEH LHTRIAQLEL QLSNAEQQKT ELQTKAAKLM 1990 2000 2010 2020 2030 2040 KRLKEYKTKA TTTATPTVTV DNDLDSTIIE ELKHQLQLQE SRLSKAEEIS QQHALEKEKL 2050 2060 2070 2080 2090 2100 AKRIDVLTAG NDRMAEMKER QDMDVQMYQA RIRELQEKLS QLDQWGEPAA TVSSSLDGDE 2110 2120 2130 2140 2150 2160 AARIESLQQE IQQLRQQVSE LEDERTRDQA ELGALRQSSQ GYDEAEDNQK LELQQLRQQE 2170 2180 2190 2200 2210 2220 SELEALRTRD QSELEALRQS CQGHDETVRI ATLQQDNQQL ELQQLRQAII ELETLRARDQ 2230 2240 2250 2260 2270 2280 TELEALRQSS QGHDEAARIA IEQRDNQQLE LQQLRQQLIE LEALRARDQA ELEALRQSCQ 2290 2300 2310 2320 2330 2340 GQQLSVDMAS RNDEQMAQLQ EKESEIVHLK QRIEELMRED QTEKLVFEIL TKNQELQLLR 2350 2360 2370 2380 2390 2400 MQVKQLEEDK EDQQVSAAPP KDDGETVEKL KSLCQQLQQE KSDMEEELRV LNNHVLSSLE 2410 2420 2430 2440 2450 2460 LEDRMKQTLL QLDTKNIEIT ELRRSLEILQ SQNLGQNSAA EQIPDLSAIN QQWEQLVEQK 2470 2480 2490 2500 2510 2520 CGEVASIWQE HLSQREAAFK AQLEEVTQQQ QRELPQSQQS TQGEATSDIM QKMQKALETQ 2530 2540 2550 2560 2570 2580 EMEIVTLKEQ LAIRSAEYAR LAAQYDPFRL QNRGGASGGN PASTTVSAGG PPSLTANEPL 2590 2600 2610 2620 2630 2640 PEYVLKADLD YALMMLHQRD MRVEEMIVEL VQLLEERDHL QLKLSDTLRQ LETERSRVSD 2650 2660 2670 2680 2690 2700 EPSATASSSA ASSSSPSKIS SAGSNSELLG TTSAAGSDLK QKLAELQTVK HSKDKVIVDE 2710 2720 2730 2740 2750 2760 REQRLQQMLQ LQKDMAKQGS GSQSGAGAVA AVAAPTSAAP TAIGVDLSQS GLRSPSMMLM 2770 DWILGNNNKE EEAGHQTTG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 980-2779. Strain: Berkeley. Tissue: Embryo.
[4]	"Lava lamp, a novel peripheral Golgi protein, is required for Drosophila melanogaster cellularization." Sisson J.C., Field C., Ventura R., Royou A., Sullivan W. J. Cell Biol. 151:905-918(2000) [PubMed: 11076973] [Abstract] Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
[5]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34; SER-35; SER-95; SER-98; SER-122; SER-133; SER-186; SER-352 AND SER-354, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014298 Genomic DNA. Translation: AAF45910.1. AY118638 mRNA. Translation: AAM50007.1. Different initiation.
RefSeq	NP_525064.1.
UniGene	Dm.1688
3D structure databases
HSSP	HSSP built from PDB template 1NO4 based on UniProtKB P13848.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:17967N.
IntAct	Q8MSS1. 8 interactions.
Genome annotation databases
Ensembl	FBgn0029688. Drosophila melanogaster. [Contig view]
GeneID	31350.
KEGG	dme:Dmel_CG6450.
NMPDR	fig|7227.3.peg.16429.
Organism-specific databases
FlyBase	FBgn0029688. lva.
Phylogenomic databases
HOGENOM	Q8MSS1.
OMA	Q8MSS1. LDVQNEK.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-000534-MON.
Gene expression databases
ArrayExpress	Q8MSS1.
GermOnline	CG6450. Drosophila melanogaster.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	773190.

Entry information

Entry name

LVA_DROME

Accession

Primary (citable) accession number: Q8MSS1
Secondary accession number(s): Q9W4N7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 9, 2003
Last sequence update:	May 9, 2003
Last modified:	June 16, 2009
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project