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Protein

Aprataxin-like protein

Gene

CG5316

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri381 – 40323C2H2-typeAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • mRNA splicing, via spliceosome Source: FlyBase
  • single strand break repair Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Aprataxin-like protein (EC:3.-.-.-)
Gene namesi
ORF Names:CG5316
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0038704. CG5316.

Subcellular locationi

GO - Cellular componenti

  • precatalytic spliceosome Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 662662Aprataxin-like proteinPRO_0000109849Add
BLAST

Proteomic databases

PaxDbiQ8MSG8.
PRIDEiQ8MSG8.

Expressioni

Gene expression databases

BgeeiFBgn0038704.
GenevisibleiQ8MSG8. DM.

Interactioni

Protein-protein interaction databases

BioGridi67317. 2 interactions.
IntActiQ8MSG8. 1 interaction.
MINTiMINT-775505.
STRINGi7227.FBpp0083172.

Structurei

3D structure databases

ProteinModelPortaliQ8MSG8.
SMRiQ8MSG8. Positions 3-176, 232-404.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 108105HITPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi538 – 647110Gln-richAdd
BLAST

Domaini

The HIT domain is required for enzymatic activity.By similarity
The C2H2-type zinc finger mediates DNA-binding.By similarity

Sequence similaritiesi

Contains 1 C2H2-type zinc finger.Curated
Contains 1 FHA-like domain.Curated
Contains 1 HIT domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri381 – 40323C2H2-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0562. Eukaryota.
ENOG41102F4. LUCA.
GeneTreeiENSGT00570000079163.
InParanoidiQ8MSG8.
KOiK10863.
OMAiPLHCNQC.
OrthoDBiEOG091G03Z8.
PhylomeDBiQ8MSG8.

Family and domain databases

Gene3Di3.30.428.10. 2 hits.
InterProiIPR026963. Aprataxin.
IPR019808. Histidine_triad_CS.
IPR011146. HIT-like.
IPR032566. Znf-C2HE.
IPR015880. Znf_C2H2-like.
[Graphical view]
PANTHERiPTHR12486:SF4. PTHR12486:SF4. 1 hit.
PfamiPF16278. zf-C2HE. 2 hits.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
SUPFAMiSSF54197. SSF54197. 2 hits.
PROSITEiPS00892. HIT_1. 1 hit.
PS51084. HIT_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: Q8MSG8-1) [UniParc]FASTAAdd to basket
Also known as: C

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSWSSALIKD ISKPENLIIS SEIAVVIADK FPKAQHHYLV LPLADIPSIF
60 70 80 90 100
HLNRSHLSLL EELHLLARNV VEVKGVRWQD FNVGFHAEPS MQRLHLHVIS
110 120 130 140 150
KDFVSTSLKT KKHWNSFNTE LFVPYTKLYA QLEKENSISR LPKSLKDELL
160 170 180 190 200
AKPLICNQCE FVARNLPSLK GHLVGHLQDP KSVCQRVRLG NQFFPTAGYR
210 220 230 240 250
TSELAYCFDF VDFYEYKKQM EVDKLAYIRD ELQRKLNDKR NFLIESDRAV
260 270 280 290 300
VMKADYPKSQ YHFRVVAKEE FRDITQLTEA QLPLLDHMMD LANQIIEKQK
310 320 330 340 350
HLESRNFLIG FKVNTFWNRL NLHVISNDFY SMAMKRISHW NSFNTELFMP
360 370 380 390 400
FQIAYMMLSV QGSIESISEE TYNNLQEKTP LRCNQCEFVT NMLLDLKAHL
410 420 430 440 450
YQHWQRKEDE RDQKKKVDKI IQMISETKLD EAEAKPKLLN EEEPIQAQPV
460 470 480 490 500
AAIAQYPNEH LGKPLTPQQQ PGKQQAQNVY DKNINGPSVN MMNQNNPNNP
510 520 530 540 550
FRNTPHLNRQ SQKPPHPRSG PRGPMAPWTG PRFPCHQQQN RFRPPGFNAC
560 570 580 590 600
RQPYPPYHSG HQQFPNASSV GGGQTGLPGQ GQGPRPKWNS NKIFNQQNRQ
610 620 630 640 650
NTVQAQPQAQ NQQTNQQQIQ NSNKNQTPKK KPWKNRLQPV GKVQNQGGAN
660
RDPAPPSNSK PS
Note: No experimental confirmation available.
Length:662
Mass (Da):76,477
Last modified:June 7, 2004 - v2
Checksum:iD4DC720076FFB331
GO
Isoform D (identifier: Q8MSG8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-90: Missing.

Note: No experimental confirmation available.
Show »
Length:572
Mass (Da):66,317
Checksum:iDF9B652A314D9902
GO
Isoform A (identifier: Q8MSG8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     127-127: K → SKAIRTRRRLQAS
     128-662: Missing.

Note: No experimental confirmation available.
Show »
Length:139
Mass (Da):16,019
Checksum:i4641400E7B2B5BFD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti44 – 441A → D in AAM50692 (PubMed:12537569).Curated
Sequence conflicti106 – 1061T → K in AAM50692 (PubMed:12537569).Curated
Sequence conflicti109 – 1091K → E in AAS93744 (Ref. 5) Curated
Sequence conflicti194 – 1941F → I in AAM50692 (PubMed:12537569).Curated
Sequence conflicti300 – 3001K → T in AAM50692 (PubMed:12537569).Curated
Sequence conflicti410 – 4101E → K in AAM50692 (PubMed:12537569).Curated
Sequence conflicti440 – 4401N → P in AAM50692 (PubMed:12537569).Curated
Sequence conflicti451 – 4522AA → GSG in AAM50692 (PubMed:12537569).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9090Missing in isoform D. 1 PublicationVSP_010547Add
BLAST
Alternative sequencei127 – 1271K → SKAIRTRRRLQAS in isoform A. 1 PublicationVSP_047935
Alternative sequencei128 – 662535Missing in isoform A. 1 PublicationVSP_047936Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF55666.2.
AE014297 Genomic DNA. Translation: AAF55667.1.
AE014297 Genomic DNA. Translation: AAN13800.1.
AY118832 mRNA. Translation: AAM50692.1.
BT011192 mRNA. Translation: AFZ88725.1.
BT012473 mRNA. Translation: AAS93744.1.
RefSeqiNP_650805.1. NM_142548.2. [Q8MSG8-1]
NP_732421.1. NM_169858.1. [Q8MSG8-1]
NP_732422.1. NM_169859.3. [Q8MSG8-4]
UniGeneiDm.13958.

Genome annotation databases

EnsemblMetazoaiFBtr0083758; FBpp0083172; FBgn0038704. [Q8MSG8-1]
FBtr0083760; FBpp0083174; FBgn0038704. [Q8MSG8-1]
GeneIDi42322.
KEGGidme:Dmel_CG5316.
UCSCiCG5316-RA. d. melanogaster. [Q8MSG8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF55666.2.
AE014297 Genomic DNA. Translation: AAF55667.1.
AE014297 Genomic DNA. Translation: AAN13800.1.
AY118832 mRNA. Translation: AAM50692.1.
BT011192 mRNA. Translation: AFZ88725.1.
BT012473 mRNA. Translation: AAS93744.1.
RefSeqiNP_650805.1. NM_142548.2. [Q8MSG8-1]
NP_732421.1. NM_169858.1. [Q8MSG8-1]
NP_732422.1. NM_169859.3. [Q8MSG8-4]
UniGeneiDm.13958.

3D structure databases

ProteinModelPortaliQ8MSG8.
SMRiQ8MSG8. Positions 3-176, 232-404.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi67317. 2 interactions.
IntActiQ8MSG8. 1 interaction.
MINTiMINT-775505.
STRINGi7227.FBpp0083172.

Proteomic databases

PaxDbiQ8MSG8.
PRIDEiQ8MSG8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0083758; FBpp0083172; FBgn0038704. [Q8MSG8-1]
FBtr0083760; FBpp0083174; FBgn0038704. [Q8MSG8-1]
GeneIDi42322.
KEGGidme:Dmel_CG5316.
UCSCiCG5316-RA. d. melanogaster. [Q8MSG8-1]

Organism-specific databases

FlyBaseiFBgn0038704. CG5316.

Phylogenomic databases

eggNOGiKOG0562. Eukaryota.
ENOG41102F4. LUCA.
GeneTreeiENSGT00570000079163.
InParanoidiQ8MSG8.
KOiK10863.
OMAiPLHCNQC.
OrthoDBiEOG091G03Z8.
PhylomeDBiQ8MSG8.

Miscellaneous databases

ChiTaRSiCG5316. fly.
GenomeRNAii42322.
PROiQ8MSG8.

Gene expression databases

BgeeiFBgn0038704.
GenevisibleiQ8MSG8. DM.

Family and domain databases

Gene3Di3.30.428.10. 2 hits.
InterProiIPR026963. Aprataxin.
IPR019808. Histidine_triad_CS.
IPR011146. HIT-like.
IPR032566. Znf-C2HE.
IPR015880. Znf_C2H2-like.
[Graphical view]
PANTHERiPTHR12486:SF4. PTHR12486:SF4. 1 hit.
PfamiPF16278. zf-C2HE. 2 hits.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
SUPFAMiSSF54197. SSF54197. 2 hits.
PROSITEiPS00892. HIT_1. 1 hit.
PS51084. HIT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPTX_DROME
AccessioniPrimary (citable) accession number: Q8MSG8
Secondary accession number(s): A4V347
, L0BID2, Q9VDX2, Q9VDX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: September 7, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.