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Protein

Aprataxin-like protein

Gene

CG5316

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri381 – 403C2H2-typeAdd BLAST23

GO - Molecular functioni

GO - Biological processi

  • mRNA splicing, via spliceosome Source: FlyBase
  • single strand break repair Source: UniProtKB

Keywordsi

Molecular functionDNA-binding, Hydrolase
Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Aprataxin-like protein (EC:3.-.-.-)
Gene namesi
ORF Names:CG5316
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0038704 CG5316

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001098491 – 662Aprataxin-like proteinAdd BLAST662

Proteomic databases

PaxDbiQ8MSG8
PRIDEiQ8MSG8

Expressioni

Gene expression databases

BgeeiFBgn0038704
ExpressionAtlasiQ8MSG8 baseline and differential
GenevisibleiQ8MSG8 DM

Interactioni

Protein-protein interaction databases

BioGridi67317, 2 interactors
IntActiQ8MSG8, 1 interactor
STRINGi7227.FBpp0083172

Structurei

3D structure databases

ProteinModelPortaliQ8MSG8
SMRiQ8MSG8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 108HITPROSITE-ProRule annotationAdd BLAST105

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi538 – 647Gln-richAdd BLAST110

Domaini

The HIT domain is required for enzymatic activity.By similarity
The C2H2-type zinc finger mediates DNA-binding.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri381 – 403C2H2-typeAdd BLAST23

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0562 Eukaryota
ENOG41102F4 LUCA
GeneTreeiENSGT00570000079163
InParanoidiQ8MSG8
KOiK10863
OMAiVEACEVR
OrthoDBiEOG091G03Z8
PhylomeDBiQ8MSG8

Family and domain databases

Gene3Di3.30.428.10, 2 hits
InterProiView protein in InterPro
IPR019808 Histidine_triad_CS
IPR011146 HIT-like
IPR036265 HIT-like_sf
IPR032566 Znf-C2HE
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF16278 zf-C2HE, 2 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 2 hits
SUPFAMiSSF54197 SSF54197, 2 hits
PROSITEiView protein in PROSITE
PS00892 HIT_1, 1 hit
PS51084 HIT_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: Q8MSG8-1) [UniParc]FASTAAdd to basket
Also known as: C

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSWSSALIKD ISKPENLIIS SEIAVVIADK FPKAQHHYLV LPLADIPSIF
60 70 80 90 100
HLNRSHLSLL EELHLLARNV VEVKGVRWQD FNVGFHAEPS MQRLHLHVIS
110 120 130 140 150
KDFVSTSLKT KKHWNSFNTE LFVPYTKLYA QLEKENSISR LPKSLKDELL
160 170 180 190 200
AKPLICNQCE FVARNLPSLK GHLVGHLQDP KSVCQRVRLG NQFFPTAGYR
210 220 230 240 250
TSELAYCFDF VDFYEYKKQM EVDKLAYIRD ELQRKLNDKR NFLIESDRAV
260 270 280 290 300
VMKADYPKSQ YHFRVVAKEE FRDITQLTEA QLPLLDHMMD LANQIIEKQK
310 320 330 340 350
HLESRNFLIG FKVNTFWNRL NLHVISNDFY SMAMKRISHW NSFNTELFMP
360 370 380 390 400
FQIAYMMLSV QGSIESISEE TYNNLQEKTP LRCNQCEFVT NMLLDLKAHL
410 420 430 440 450
YQHWQRKEDE RDQKKKVDKI IQMISETKLD EAEAKPKLLN EEEPIQAQPV
460 470 480 490 500
AAIAQYPNEH LGKPLTPQQQ PGKQQAQNVY DKNINGPSVN MMNQNNPNNP
510 520 530 540 550
FRNTPHLNRQ SQKPPHPRSG PRGPMAPWTG PRFPCHQQQN RFRPPGFNAC
560 570 580 590 600
RQPYPPYHSG HQQFPNASSV GGGQTGLPGQ GQGPRPKWNS NKIFNQQNRQ
610 620 630 640 650
NTVQAQPQAQ NQQTNQQQIQ NSNKNQTPKK KPWKNRLQPV GKVQNQGGAN
660
RDPAPPSNSK PS
Note: No experimental confirmation available.
Length:662
Mass (Da):76,477
Last modified:June 7, 2004 - v2
Checksum:iD4DC720076FFB331
GO
Isoform D (identifier: Q8MSG8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-90: Missing.

Note: No experimental confirmation available.
Show »
Length:572
Mass (Da):66,317
Checksum:iDF9B652A314D9902
GO
Isoform A (identifier: Q8MSG8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     127-127: K → SKAIRTRRRLQAS
     128-662: Missing.

Note: No experimental confirmation available.
Show »
Length:139
Mass (Da):16,019
Checksum:i4641400E7B2B5BFD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44A → D in AAM50692 (PubMed:12537569).Curated1
Sequence conflicti106T → K in AAM50692 (PubMed:12537569).Curated1
Sequence conflicti109K → E in AAS93744 (Ref. 5) Curated1
Sequence conflicti194F → I in AAM50692 (PubMed:12537569).Curated1
Sequence conflicti300K → T in AAM50692 (PubMed:12537569).Curated1
Sequence conflicti410E → K in AAM50692 (PubMed:12537569).Curated1
Sequence conflicti440N → P in AAM50692 (PubMed:12537569).Curated1
Sequence conflicti451 – 452AA → GSG in AAM50692 (PubMed:12537569).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0105471 – 90Missing in isoform D. 1 PublicationAdd BLAST90
Alternative sequenceiVSP_047935127K → SKAIRTRRRLQAS in isoform A. 1 Publication1
Alternative sequenceiVSP_047936128 – 662Missing in isoform A. 1 PublicationAdd BLAST535

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA Translation: AAF55666.2
AE014297 Genomic DNA Translation: AAF55667.1
AE014297 Genomic DNA Translation: AAN13800.1
AY118832 mRNA Translation: AAM50692.1
BT011192 mRNA Translation: AFZ88725.1
BT012473 mRNA Translation: AAS93744.1
RefSeqiNP_650805.1, NM_142548.2 [Q8MSG8-1]
NP_732421.1, NM_169858.1 [Q8MSG8-1]
NP_732422.1, NM_169859.3 [Q8MSG8-4]
UniGeneiDm.13958

Genome annotation databases

EnsemblMetazoaiFBtr0083758; FBpp0083172; FBgn0038704 [Q8MSG8-1]
FBtr0083759; FBpp0083173; FBgn0038704 [Q8MSG8-4]
FBtr0083760; FBpp0083174; FBgn0038704 [Q8MSG8-1]
GeneIDi42322
KEGGidme:Dmel_CG5316
UCSCiCG5316-RA d. melanogaster [Q8MSG8-1]
CG5316-RC d. melanogaster

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAPTX_DROME
AccessioniPrimary (citable) accession number: Q8MSG8
Secondary accession number(s): A4V347
, L0BID2, Q9VDX2, Q9VDX3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: May 23, 2018
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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