Reviewed, UniProtKB/Swiss-Prot Q8MR31 (SLIP1_DROME)

Names and origin

Protein names

Recommended name:
    Slo-interacting protein 1
Alternative name(s):
    dSLIP1

Gene names

Name:	Slip1
ORF Names:	CG1783

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	767 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May selectively reduce calcium-activated potassium channel (Slo) currents by reducing the number of Slo channels in the plasma membrane. Ref.1
Subunit structure	Interacts with Slo. Ref.1
Tissue specificity	In embryos, it is expressed throughout the CNS and in several peripheral locations. Colocalizes with Slo. Ref.1
Sequence similarities	Contains 1 PDZ (DHR) domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	potassium channel regulator activity Ref.1 Inferred from direct assay. Source: UniProtKB protein binding Ref.1 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
slo	Q03720	4	EBI-123875,EBI-426805

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8MR31-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8MR31-2)

The sequence of this isoform differs from the canonical sequence as follows:
     215-520: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 767	767	Slo-interacting protein 1		PRO_0000071957
Regions
	Domain	202 – 280	79	PDZ
Natural variations
	Alternative sequence	215 – 520	306	Missing in isoform 2.		VSP_010098

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 26, 2004. Version 2. Checksum: 8914206A7C34E46F	FASTA	767	85,846
	10 20 30 40 50 60 MSIADVEYEY VVLKINGYDI SHLSRYEAVQ KFLQSKETLV VEIRRQKHNA LDLELKHGSN 70 80 90 100 110 120 AKISKVDNPG ELSVLTDKSA EGTITAASAS QQINCPSSTS LKEIETKTPV VLTLRARSHE 130 140 150 160 170 180 DRLGSLQAAS KETQTQSVVG TDVLKDNDLV NTITDNFIEH EHHLFEQCLE PEIDIEEVTL 190 200 210 220 230 240 VKGVEQSSSN QIGLIVTSSG IQQSSTDTNK GDILGNVLEH SEDVFISGVQ PESIAYRDGR 250 260 270 280 290 300 LRQGDQILRI NGLDVKNQEE LETQIARSST SVTLLVSRIL YPEDDDDEDI HFEYANTFLP 310 320 330 340 350 360 DDYTNVVDKL DKVLLTHVKS LEELSNKSAM QSDECYHIPE KNSSDSNVKI SSLAKNIIEQ 370 380 390 400 410 420 STKSCSKIKL RPNANLDYKK KFNLPLSQEE VHLQYEYDES EHIYETIPED SESEPVYCSP 430 440 450 460 470 480 YQRSNDKTSI GCSSPIASRP AESLERTMQQ QTQRVAQWLG LKPQYQKTRQ TLVGRPPPLK 490 500 510 520 530 540 LVQQPTCSRV FTLRSTLTNT SASSSSGVAY SSYGQNNVVT GNAAAPGEEV DNSSSAYNTG 550 560 570 580 590 600 DSNNSASPHQ NTTNPDEAIA TGRKLDSTVI DSPNDHLDAT GVSTMLLLPF GKSGRIGLCS 610 620 630 640 650 660 SNLPTAYVSE RYTNVGSENE IHPLKSDIEI LRVKPTDDSY SHCPQFNAPN LSSYHFVSSQ 670 680 690 700 710 720 EVANRCHIST SLQKNATLLN GESAEEIPMV WKVKRRPDGT RYIVKRPVRN RPQVALRKNM 730 740 750 760 RYNEVTTTED DTISEVKIGR YWTKEERKRH IERAREKRHH QTQQQQQ
	Isoform 2. Checksum: C9849AF8BCB605FE	FASTA	461	51,273
	10 20 30 40 50 60 MSIADVEYEY VVLKINGYDI SHLSRYEAVQ KFLQSKETLV VEIRRQKHNA LDLELKHGSN 70 80 90 100 110 120 AKISKVDNPG ELSVLTDKSA EGTITAASAS QQINCPSSTS LKEIETKTPV VLTLRARSHE 130 140 150 160 170 180 DRLGSLQAAS KETQTQSVVG TDVLKDNDLV NTITDNFIEH EHHLFEQCLE PEIDIEEVTL 190 200 210 220 230 240 VKGVEQSSSN QIGLIVTSSG IQQSSTDTNK GDILGNAAAP GEEVDNSSSA YNTGDSNNSA 250 260 270 280 290 300 SPHQNTTNPD EAIATGRKLD STVIDSPNDH LDATGVSTML LLPFGKSGRI GLCSSNLPTA 310 320 330 340 350 360 YVSERYTNVG SENEIHPLKS DIEILRVKPT DDSYSHCPQF NAPNLSSYHF VSSQEVANRC 370 380 390 400 410 420 HISTSLQKNA TLLNGESAEE IPMVWKVKRR PDGTRYIVKR PVRNRPQVAL RKNMRYNEVT 430 440 450 460 TTEDDTISEV KIGRYWTKEE RKRHIERARE KRHHQTQQQQ Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"dSLo interacting protein 1, a novel protein that interacts with large-conductance calcium-activated potassium channels." Xia X.-M., Hirschberg B., Smolik S., Forte M., Adelman J.P. J. Neurosci. 18:2360-2369(1998) [PubMed: 9502797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH SLO. Tissue: Head.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: Berkeley. Tissue: Embryo.
[5]	"Nucleotide variation along the Drosophila melanogaster fourth chromosome." Wang W., Thornton K., Berry A., Long M. Science 295:134-137(2002) [PubMed: 11778050] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 252-282. Strain: 253.27 and 253.30.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014135 Genomic DNA. Translation: AAF59321.1. AY122157 mRNA. Translation: AAM52669.1. Different initiation. AF433691 Genomic DNA. Translation: AAM17954.1. AF433692 Genomic DNA. Translation: AAM17955.1.
RefSeq	NP_651933.1.
UniGene	Dm.11434
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8MR31. 8 interactions.
Genome annotation databases
Ensembl	FBgn0024728. Drosophila melanogaster. [Contig view]
GeneID	43809.
KEGG	dme:Dmel_CG1783.
Organism-specific databases
FlyBase	FBgn0024728. Slip1.
Phylogenomic databases
HOGENOM	Q8MR31.
OMA	Q8MR31. DESEHIY.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-018029-MON.
Gene expression databases
ArrayExpress	Q8MR31.
GermOnline	CG1783. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001478. PDZ/DHR/GLGF. [Graphical view]
Pfam	PF00595. PDZ. 1 hit. [Graphical view]
SMART	SM00228. PDZ. 1 hit. [Graphical view]
PROSITE	PS50106. PDZ. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	835990.

Entry information

Entry name

SLIP1_DROME

Accession

Primary (citable) accession number: Q8MR31
Secondary accession number(s): Q8STH5, Q9V4F2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	April 26, 2004
Last modified:	June 16, 2009
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project