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Q8MQU6 (CISYP_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Citrate synthase, peroxisomal

EC=2.3.3.1
Gene names
Name:cshA
ORF Names:DDB_G0267426
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Peroxisomal protein involved in the cellular biosynthesis of citrate, and required primarily for cell growth and modulation of multicellular development. Ref.1

Catalytic activity

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.

Subcellular location

Peroxisome Ref.1.

Developmental stage

Maximally expressed during vegetative growth and gradually decreased through subsequent developmental stages. Ref.1

Sequence similarities

Belongs to the citrate synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Peroxisome Potential
Chain? – 492Citrate synthase, peroxisomalPRO_0000327837

Sites

Active site3071 By similarity
Active site3461 By similarity
Active site4021 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8MQU6 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 2008B6988B19A597

FASTA49255,749
        10         20         30         40         50         60 
MAHIDRINIL ANHLSKEEEE ENELLTSPVS ADKKKETVTV TDNRNGKSYD FKIKNDTINA 

        70         80         90        100        110        120 
LNFKELQLAK GDGGLMIYDP GFQNTAVVTS YITYIDGDKG ILRYRGYPIE ELAERSNFLE 

       130        140        150        160        170        180 
VAYLLINGNL PNKSQLDGWS NKIMTHTFLH ENLVGLMKTF RYDAHPMGML ISTVAALGTF 

       190        200        210        220        230        240 
YPEANPALAG GDIFKSENVR NKQIYRIVGK LPTIAACAWR HRIGRPYNTP VNHLGYTENF 

       250        260        270        280        290        300 
LYMLDKLSEP DYKPNPVLCR ALEILFILHA DHELNCSTAA MRHISSSGTD PYTSVAGAAG 

       310        320        330        340        350        360 
ALYGPSHGGA NEAVLDMLIH IGSKENIPQF ISDVKSKKKK LMGFGHRIYK NYDPRAKIIR 

       370        380        390        400        410        420 
RVAYEVFESL GKEPLIEVAT ELEKQALEDE YFVSRKLYPN VDFYSGLIYK AMGFPTDMFP 

       430        440        450        460        470        480 
VLFTIPRAVG WLAHWVEHLE DPETKIYRPR QVYKGEWFRN YVPIDGRPPA KVRSQDSYSS 

       490 
ATTKRYSKVT SH 

« Hide

References

« Hide 'large scale' references
[1]"Disruption of the peroxisomal citrate synthase CshA affects cell growth and multicellular development in Dictyostelium discoideum."
Huang Y.-C., Chen Y.-H., Lo S.-R., Liu C.-I., Wang C.-W., Chang W.-T.
Mol. Microbiol. 53:81-91(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
Strain: AX2.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY124377 mRNA. Translation: AAM92490.1.
AAFI02000003 Genomic DNA. Translation: EAL73165.1.

3D structure databases

ProteinModelPortalQ8MQU6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0191110.

Proteomic databases

PRIDEQ8MQU6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0191110; DDB0191110; DDB_G0267426.
KEGGddi:DDB_G0267426.

Organism-specific databases

dictyBaseDDB_G0267426. cshA.

Phylogenomic databases

eggNOGCOG0372.
KOK01647.
OMAINILANH.
PhylomeDBQ8MQU6.
ProtClustDBCLSZ2430014.

Enzyme and pathway databases

UniPathwayUPA00223; UER00717.

Family and domain databases

Gene3D1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
IPR010953. Citrate_synthase_typ-II.
[Graphical view]
PANTHERPTHR11739. PTHR11739. 1 hit.
PfamPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFPIRSF001369. Citrate_synth. 1 hit.
PRINTSPR00143. CITRTSNTHASE.
SUPFAMSSF48256. SSF48256. 1 hit.
TIGRFAMsTIGR01798. cit_synth_I. 1 hit.
PROSITEPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCISYP_DICDI
AccessionPrimary (citable) accession number: Q8MQU6
Secondary accession number(s): Q55FD7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase