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Protein

Citrate synthase, peroxisomal

Gene

cshA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Peroxisomal protein involved in the cellular biosynthesis of citrate, and required primarily for cell growth and modulation of multicellular development.1 Publication

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Pathway:itricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase, mitochondrial (cs), Citrate synthase (gltA), Citrate synthase, peroxisomal (cshA)
  2. Probable aconitate hydratase, mitochondrial (aco2)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei307 – 3071PROSITE-ProRule annotation
Active sitei346 – 3461PROSITE-ProRule annotation
Active sitei402 – 4021PROSITE-ProRule annotation

GO - Molecular functioni

  • citrate (Si)-synthase activity Source: dictyBase

GO - Biological processi

  • asexual reproduction Source: dictyBase
  • endocytosis Source: dictyBase
  • glyoxylate cycle Source: dictyBase
  • phagocytosis Source: dictyBase
  • pinocytosis Source: dictyBase
  • positive regulation of phagocytosis Source: dictyBase
  • sorocarp morphogenesis Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase, peroxisomal (EC:2.3.3.16)
Gene namesi
Name:cshA
ORF Names:DDB_G0267426
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 1, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0267426. cshA.

Subcellular locationi

GO - Cellular componenti

  • peroxisome Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 492Citrate synthase, peroxisomalPRO_0000327837
Transit peptidei1 – ?PeroxisomeSequence Analysis

Proteomic databases

PRIDEiQ8MQU6.

Expressioni

Developmental stagei

Maximally expressed during vegetative growth and gradually decreased through subsequent developmental stages.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0191110.

Structurei

3D structure databases

ProteinModelPortaliQ8MQU6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0372.
InParanoidiQ8MQU6.
KOiK01647.
OMAiYADHEVP.
PhylomeDBiQ8MQU6.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase.
IPR019810. Citrate_synthase_AS.
IPR010953. Citrate_synthase_typ-I.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01798. cit_synth_I. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8MQU6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHIDRINIL ANHLSKEEEE ENELLTSPVS ADKKKETVTV TDNRNGKSYD
60 70 80 90 100
FKIKNDTINA LNFKELQLAK GDGGLMIYDP GFQNTAVVTS YITYIDGDKG
110 120 130 140 150
ILRYRGYPIE ELAERSNFLE VAYLLINGNL PNKSQLDGWS NKIMTHTFLH
160 170 180 190 200
ENLVGLMKTF RYDAHPMGML ISTVAALGTF YPEANPALAG GDIFKSENVR
210 220 230 240 250
NKQIYRIVGK LPTIAACAWR HRIGRPYNTP VNHLGYTENF LYMLDKLSEP
260 270 280 290 300
DYKPNPVLCR ALEILFILHA DHELNCSTAA MRHISSSGTD PYTSVAGAAG
310 320 330 340 350
ALYGPSHGGA NEAVLDMLIH IGSKENIPQF ISDVKSKKKK LMGFGHRIYK
360 370 380 390 400
NYDPRAKIIR RVAYEVFESL GKEPLIEVAT ELEKQALEDE YFVSRKLYPN
410 420 430 440 450
VDFYSGLIYK AMGFPTDMFP VLFTIPRAVG WLAHWVEHLE DPETKIYRPR
460 470 480 490
QVYKGEWFRN YVPIDGRPPA KVRSQDSYSS ATTKRYSKVT SH
Length:492
Mass (Da):55,749
Last modified:October 1, 2002 - v1
Checksum:i2008B6988B19A597
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY124377 mRNA. Translation: AAM92490.1.
AAFI02000003 Genomic DNA. Translation: EAL73165.1.
RefSeqiXP_647596.1. XM_642504.1.

Genome annotation databases

EnsemblProtistsiDDB0191110; DDB0191110; DDB_G0267426.
GeneIDi8616408.
KEGGiddi:DDB_G0267426.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY124377 mRNA. Translation: AAM92490.1.
AAFI02000003 Genomic DNA. Translation: EAL73165.1.
RefSeqiXP_647596.1. XM_642504.1.

3D structure databases

ProteinModelPortaliQ8MQU6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0191110.

Proteomic databases

PRIDEiQ8MQU6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0191110; DDB0191110; DDB_G0267426.
GeneIDi8616408.
KEGGiddi:DDB_G0267426.

Organism-specific databases

dictyBaseiDDB_G0267426. cshA.

Phylogenomic databases

eggNOGiCOG0372.
InParanoidiQ8MQU6.
KOiK01647.
OMAiYADHEVP.
PhylomeDBiQ8MQU6.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.

Miscellaneous databases

PROiQ8MQU6.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase.
IPR019810. Citrate_synthase_AS.
IPR010953. Citrate_synthase_typ-I.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01798. cit_synth_I. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Disruption of the peroxisomal citrate synthase CshA affects cell growth and multicellular development in Dictyostelium discoideum."
    Huang Y.-C., Chen Y.-H., Lo S.-R., Liu C.-I., Wang C.-W., Chang W.-T.
    Mol. Microbiol. 53:81-91(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    Strain: AX2.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiCISYP_DICDI
AccessioniPrimary (citable) accession number: Q8MQU6
Secondary accession number(s): Q55FD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 1, 2002
Last modified: July 22, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.