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Protein

Brain tumor protein

Gene

brat

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a central role in translation repression of hb mRNA by being recruited by nos and pum to the Nanos Response Element (NRE), a 16 bp sequence in the hb mRNA 3'-UTR. Probably recruited by other proteins to repress translation of other mRNAs in other tissues. Involved in the regulation of ribosomal RNA synthesis and cell growth. Participates in abdominal segmentation and imaginal disk development. Involved in brain tumor suppression, by acting as a growth suppressor in the larval brain.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri174 – 222B box-type 1PROSITE-ProRule annotationAdd BLAST49
Zinc fingeri323 – 366B box-type 2PROSITE-ProRule annotationAdd BLAST44

GO - Molecular functioni

  • translation regulator activity Source: FlyBase
  • translation repressor activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • asymmetric cell division Source: FlyBase
  • asymmetric neuroblast division resulting in ganglion mother cell formation Source: FlyBase
  • axon guidance Source: FlyBase
  • brain development Source: FlyBase
  • ganglion mother cell fate determination Source: FlyBase
  • negative regulation of cell proliferation Source: FlyBase
  • negative regulation of neuroblast proliferation Source: FlyBase
  • neuroblast fate determination Source: FlyBase
  • neuroblast proliferation Source: FlyBase
  • neurogenesis Source: FlyBase
  • neuron differentiation Source: FlyBase
  • oogenesis Source: FlyBase
  • phagocytosis Source: FlyBase
  • regulation of neurogenesis Source: FlyBase
  • regulation of synaptic growth at neuromuscular junction Source: FlyBase
  • regulation of synaptic vesicle endocytosis Source: FlyBase
  • regulation of translation Source: FlyBase
  • rRNA transcription Source: UniProtKB
  • segmentation Source: FlyBase
  • ventral cord development Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Translation regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Brain tumor protein
Gene namesi
Name:brat
ORF Names:CG10719
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0010300. brat.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • neuronal cell body Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi802H → L in bratfs3; induces production of tumor-like neoplasms in the larval brain. Disrupts interaction with nos and pum. 1
Mutagenesisi829Y → A: Disrupts recruitment by pum. 1 Publication1
Mutagenesisi847R → A: Disrupts recruitment by pum. 1 Publication1
Mutagenesisi859Y → A: Does not affect recruitment by pum. 1 Publication1
Mutagenesisi860G → D in bratts1; induces production of tumor-like neoplasms in the larval brain. Disrupts interaction with nos and pum. 1
Mutagenesisi875R → A: Disrupts recruitment by pum. 1 Publication1
Mutagenesisi970E → A: Does not affect recruitment by pum. 1 Publication1
Mutagenesisi1012D → A: Does not affect recruitment by pum. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002203681 – 1037Brain tumor proteinAdd BLAST1037

Proteomic databases

PaxDbiQ8MQJ9.
PRIDEiQ8MQJ9.

Expressioni

Tissue specificityi

Expressed during embryogenesis, mainly in nervous tissues. Expressed in the embryonic central and peripheral nervous systems including the embryonic brain. In third instar larva it is expressed in the larval central nervous system including the brain and the ventral ganglion, in two glands (the ring gland and the salivary gland, and in parts of the foregut) the gastric caeca and the proventriculus.1 Publication

Gene expression databases

BgeeiFBgn0010300.
ExpressionAtlasiQ8MQJ9. baseline.
GenevisibleiQ8MQJ9. DM.

Interactioni

Subunit structurei

Interacts with nos and pum. Acts via the formation of a quaternary complex composed of pum, nos, brat and the 3'-UTR mRNA of hb. Not recruited by nos and pum to cyclin B 3'-UTR mRNA.1 Publication

Protein-protein interaction databases

BioGridi61181. 3 interactors.
DIPiDIP-37857N.
IntActiQ8MQJ9. 3 interactors.
MINTiMINT-1602052.
STRINGi7227.FBpp0293081.

Structurei

Secondary structure

11037
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi762 – 764Combined sources3
Beta strandi766 – 770Combined sources5
Beta strandi773 – 776Combined sources4
Beta strandi779 – 781Combined sources3
Beta strandi783 – 788Combined sources6
Beta strandi794 – 798Combined sources5
Helixi799 – 801Combined sources3
Beta strandi803 – 807Combined sources5
Beta strandi813 – 817Combined sources5
Beta strandi820 – 823Combined sources4
Beta strandi827 – 835Combined sources9
Turni837 – 839Combined sources3
Beta strandi842 – 846Combined sources5
Helixi848 – 850Combined sources3
Beta strandi852 – 856Combined sources5
Beta strandi862 – 866Combined sources5
Turni868 – 870Combined sources3
Beta strandi874 – 879Combined sources6
Beta strandi885 – 889Combined sources5
Turni890 – 893Combined sources4
Beta strandi894 – 898Combined sources5
Beta strandi904 – 909Combined sources6
Turni911 – 913Combined sources3
Beta strandi915 – 922Combined sources8
Beta strandi924 – 932Combined sources9
Helixi933 – 935Combined sources3
Beta strandi937 – 942Combined sources6
Beta strandi947 – 952Combined sources6
Turni954 – 956Combined sources3
Beta strandi959 – 965Combined sources7
Beta strandi971 – 975Combined sources5
Beta strandi977 – 979Combined sources3
Beta strandi981 – 985Combined sources5
Beta strandi991 – 999Combined sources9
Beta strandi1004 – 1010Combined sources7
Turni1011 – 1013Combined sources3
Beta strandi1014 – 1019Combined sources6
Turni1020 – 1022Combined sources3
Beta strandi1023 – 1028Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q7FX-ray1.95A/B756-1037[»]
4ZLRX-ray2.30A/B756-1037[»]
5EX7X-ray2.60A758-1037[»]
ProteinModelPortaliQ8MQJ9.
SMRiQ8MQJ9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8MQJ9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati767 – 810NHL 1Add BLAST44
Repeati814 – 859NHL 2Add BLAST46
Repeati860 – 901NHL 3Add BLAST42
Repeati902 – 944NHL 4Add BLAST43
Repeati945 – 988NHL 5Add BLAST44

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 175Ser-richAdd BLAST173
Compositional biasi259 – 325Gln-richAdd BLAST67
Compositional biasi579 – 600His-richAdd BLAST22

Domaini

The NHL repeats form six-bladed beta-propeller that mediate the interaction with the pumilio repeats of pum, and are essential for translational effector function.1 Publication

Sequence similaritiesi

Contains 2 B box-type zinc fingers.PROSITE-ProRule annotation
Contains 5 NHL repeats.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri174 – 222B box-type 1PROSITE-ProRule annotationAdd BLAST49
Zinc fingeri323 – 366B box-type 2PROSITE-ProRule annotationAdd BLAST44

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2177. Eukaryota.
ENOG410XQJ4. LUCA.
GeneTreeiENSGT00550000074377.
InParanoidiQ8MQJ9.
KOiK11997.
OrthoDBiEOG091G02UY.
PhylomeDBiQ8MQJ9.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR001258. NHL_repeat.
IPR013017. NHL_repeat_subgr.
IPR000315. Znf_B-box.
[Graphical view]
PfamiPF01436. NHL. 4 hits.
PF00643. zf-B_box. 1 hit.
[Graphical view]
SMARTiSM00336. BBOX. 2 hits.
[Graphical view]
PROSITEiPS51125. NHL. 5 hits.
PS50119. ZF_BBOX. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8MQJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSPTPSLD SMRGGANSIE SYEHAGYLSD SPLTLSGSSP PASDSAICSD
60 70 80 90 100
EYTGGSSVKS RSEVTVINGH HPISASVSSS SSASSSSCSS SSSSSSSSSS
110 120 130 140 150
SSSSTSGLSG CGSTSSSVIS ANNVASSNGP GVIGSNLQSS NNGGNSGISS
160 170 180 190 200
LVVGAGKGSN SSSNSSSSNT SANGSPPRCT ACKSKCSDAV AKCFECQSYL
210 220 230 240 250
CANCVTAHEF MHCFNGHNVC LIKGFEASTT GTPLSVGSPS NNPASNEFKY
260 270 280 290 300
ASSLTMMLQQ QQQLDSQQQQ QQQQLLPAQP MSQLSKIVLA AAAQANSQEQ
310 320 330 340 350
QREDSIYGSL HPQQQQQQQQ QQQRQLFCPR HKQELLKFSC RTCCILVCKE
360 370 380 390 400
CIVLEHSTGL HELENVQSPG MTTSTGSTAN ESALQTLLAD MRGKIGEIVG
410 420 430 440 450
IAGNSDQNLT KVKLQYQKAH NELNETHQFF ASMLDERKTE LLKELETLYT
460 470 480 490 500
AKVNSNNSWQ QRSRDLIDKG LATCEAVERS PAPPSSLLTE ALLLRKSLEQ
510 520 530 540 550
QLQTGIQEMQ LPFEIEFMSN YQSIQAGVRN TFGYIRANSS DGGPTGMSLT
560 570 580 590 600
SNGHGKQPPI ARPTQSASNS SASSAGSGHH GHHQQSHHHG HHNHHQTAHH
610 620 630 640 650
QQLQAQSSLH GLGLGLSGAS LLDSSSSAGG AVGAFSNGGL LLGGRDRNAL
660 670 680 690 700
AVEQHFGELM PKRGGGGYTG SNGSATSAVA HYNPYEKWSN GGSDNLFSSV
710 720 730 740 750
TSGVSGSSAV ADAFASLSAV GGSVVSGAGA GGSTVSSESL LDLTNKLLSA
760 770 780 790 800
TIYPPKSQIK RQKMIYHCKF GEFGVMEGQF TEPSGVAVNA QNDIIVADTN
810 820 830 840 850
NHRIQIFDKE GRFKFQFGEC GKRDSQLLYP NRVAVVRNSG DIIVTERSPT
860 870 880 890 900
HQIQIYNQYG QFVRKFGATI LQHPRGVTVD NKGRIIVVEC KVMRVIIFDQ
910 920 930 940 950
NGNVLHKFGC SKHLEFPNGV VVNDKQEIFI SDNRAHCVKV FNYEGQYLRQ
960 970 980 990 1000
IGGEGITNYP IGVGINSNGE ILIADNHNNF NLTIFTQDGQ LISALESKVK
1010 1020 1030
HAQCFDVALM DDGSVVLASK DYRLYIYRYV QLAPVGM
Length:1,037
Mass (Da):110,366
Last modified:February 2, 2004 - v2
Checksum:i6B829CD924139DDF
GO

Sequence cautioni

The sequence AAM76183 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti591H → Y in ABA86472 (PubMed:16120803).Curated1
Sequence conflicti643G → S in AAF13928 (PubMed:10949924).Curated1
Sequence conflicti643G → S in AAF13929 (PubMed:10949924).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF119332 mRNA. Translation: AAF03086.1.
AF195870 Genomic DNA. Translation: AAF13926.1.
AF195871 Genomic DNA. Translation: AAF13927.1.
AF195872 Genomic DNA. Translation: AAF13928.1.
AF195873 Genomic DNA. Translation: AAF13929.1.
AB022432 mRNA. Translation: BAA82071.1.
AE014134 Genomic DNA. Translation: AAN11027.1.
AE014134 Genomic DNA. Translation: AAS64719.1.
AY122207 mRNA. Translation: AAM52719.1.
AY129441 mRNA. Translation: AAM76183.1. Different initiation.
DQ138866 Genomic DNA. Translation: ABA86472.1.
RefSeqiNP_001286083.1. NM_001299154.1.
NP_476945.1. NM_057597.4.
NP_599129.1. NM_134302.2.
NP_995726.1. NM_206004.2.
UniGeneiDm.6426.

Genome annotation databases

EnsemblMetazoaiFBtr0081158; FBpp0080702; FBgn0010300.
FBtr0081159; FBpp0080703; FBgn0010300.
FBtr0081160; FBpp0089239; FBgn0010300.
FBtr0344846; FBpp0311161; FBgn0010300.
GeneIDi35197.
KEGGidme:Dmel_CG10719.
UCSCiCG10719-RC. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF119332 mRNA. Translation: AAF03086.1.
AF195870 Genomic DNA. Translation: AAF13926.1.
AF195871 Genomic DNA. Translation: AAF13927.1.
AF195872 Genomic DNA. Translation: AAF13928.1.
AF195873 Genomic DNA. Translation: AAF13929.1.
AB022432 mRNA. Translation: BAA82071.1.
AE014134 Genomic DNA. Translation: AAN11027.1.
AE014134 Genomic DNA. Translation: AAS64719.1.
AY122207 mRNA. Translation: AAM52719.1.
AY129441 mRNA. Translation: AAM76183.1. Different initiation.
DQ138866 Genomic DNA. Translation: ABA86472.1.
RefSeqiNP_001286083.1. NM_001299154.1.
NP_476945.1. NM_057597.4.
NP_599129.1. NM_134302.2.
NP_995726.1. NM_206004.2.
UniGeneiDm.6426.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q7FX-ray1.95A/B756-1037[»]
4ZLRX-ray2.30A/B756-1037[»]
5EX7X-ray2.60A758-1037[»]
ProteinModelPortaliQ8MQJ9.
SMRiQ8MQJ9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61181. 3 interactors.
DIPiDIP-37857N.
IntActiQ8MQJ9. 3 interactors.
MINTiMINT-1602052.
STRINGi7227.FBpp0293081.

Proteomic databases

PaxDbiQ8MQJ9.
PRIDEiQ8MQJ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0081158; FBpp0080702; FBgn0010300.
FBtr0081159; FBpp0080703; FBgn0010300.
FBtr0081160; FBpp0089239; FBgn0010300.
FBtr0344846; FBpp0311161; FBgn0010300.
GeneIDi35197.
KEGGidme:Dmel_CG10719.
UCSCiCG10719-RC. d. melanogaster.

Organism-specific databases

CTDi35197.
FlyBaseiFBgn0010300. brat.

Phylogenomic databases

eggNOGiKOG2177. Eukaryota.
ENOG410XQJ4. LUCA.
GeneTreeiENSGT00550000074377.
InParanoidiQ8MQJ9.
KOiK11997.
OrthoDBiEOG091G02UY.
PhylomeDBiQ8MQJ9.

Miscellaneous databases

ChiTaRSibrat. fly.
EvolutionaryTraceiQ8MQJ9.
GenomeRNAii35197.
PROiQ8MQJ9.

Gene expression databases

BgeeiFBgn0010300.
ExpressionAtlasiQ8MQJ9. baseline.
GenevisibleiQ8MQJ9. DM.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR001258. NHL_repeat.
IPR013017. NHL_repeat_subgr.
IPR000315. Znf_B-box.
[Graphical view]
PfamiPF01436. NHL. 4 hits.
PF00643. zf-B_box. 1 hit.
[Graphical view]
SMARTiSM00336. BBOX. 2 hits.
[Graphical view]
PROSITEiPS51125. NHL. 5 hits.
PS50119. ZF_BBOX. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBRAT_DROME
AccessioniPrimary (citable) accession number: Q8MQJ9
Secondary accession number(s): A4V0U9
, Q0E8P2, Q2XXZ6, Q8MR02, Q9U633, Q9U634, Q9U635, Q9U636, Q9Y1W4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 2, 2004
Last modified: November 30, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.