Q8MP20 (SYTC2_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable threonine--tRNA ligase 2, cytoplasmic
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS2
ORF Names:	DDB_G0274545

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	698 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Inferred from direct assay. Source: dictyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 698	698	Probable threonine--tRNA ligase 2, cytoplasmic		PRO_0000333832
Regions
	Compositional bias	37 – 40	4	Poly-Gly
	Compositional bias	541 – 550	10	Poly-Asn
	Compositional bias	554 – 560	7	Poly-Asn

Sequences

	Sequence		Length	Mass (Da)
	Q8MP20 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: C08DC679012694A1	FASTA	698	81,318
	10 20 30 40 50 60 MSFLKLTKSI QKINNFNNNK INLINKYFST TNINNSGGGG NIKLNDGRIF KFEDKQTPLT 70 80 90 100 110 120 IANKINKTIG KQSILSRLNG NKLISMKEII EMSGKDYNIE FLNFEEHSDA RICFWNSSSL 130 140 150 160 170 180 VLARATMEYF KNENKEIELI NFGHLVNPQT ADNINQGTFY IDIFFKDKNQ TIKDNDINKI 190 200 210 220 230 240 KKIMEYIVKR NDKFEILKND QQDQDQDQEE SFIKFGEFKF KNNFLTIDSS NSIVSLDLIK 250 260 270 280 290 300 NSSVVGPNKE YSELQRIVGI SFPSKDQMNN WVEIQKIAAL RDHRVIGKDQ ELFFFHPFSP 310 320 330 340 350 360 GSCFFLPHGT KIYNKLLQFL RLEYRKRGYQ EVISPNIYNQ KLWETSGHWD NYKDNMFSFE 370 380 390 400 410 420 CDHTQYSLKP MNCPGHCLMY AHRARSYKEL PMRIADFGVL HRNETHGSLS GLTRVRRFQQ 430 440 450 460 470 480 DDAHIFCTPD MIREEIKQCL DFMKYVYTIF NFTFHLELST RPDSYLGELS VWEKAETSLS 490 500 510 520 530 540 QVLTEFCGDK WTINHGDGAF YGPKIDIHLK DANGKNHQCA TIQLDFQLPI RFNLEYSGGL 550 560 570 580 590 600 NNNNNNNNNN EEINDNNNNN SLNRPVMIHR ALFGSVERMM AILMEHTAGK WPFWLSPRQC 610 620 630 640 650 660 IVIPVSNKFN QFAQEIQSKI NLAGYDVDVD LNDSKLLSKK IREATVSQYN YIIVVGQEEI 670 680 690 DTNILNVRKR DLPEDNKQVK LSLNDLFSEF KLNIEQFK

References

[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC123513 Genomic DNA. Translation: AAM44378.1. AAFI02000012 Genomic DNA. Translation: EAL70165.1.
RefSeq	XP_644046.1. XM_638954.1.
3D structure databases
HSSP	HSSP built from PDB template 1EVL based on UniProtKB P0A8M3.
ProteinModelPortal	Q8MP20.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8MP20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231249; DDB0231249; DDB_G0274545.
GeneID	8619476.
GenomeReviews	Gene locus thrS2 in contig CM000151_GR.
KEGG	ddi:DDB_G0274545.
Organism-specific databases
dictyBase	DDB_G0274545. thrS2.
Phylogenomic databases
eggNOG	KOG1637.
GeneTree	EPrGT00050000002539.
HOGENOM	HBG352811.
OMA	REWVISA.
PhylomeDB	Q8MP20.
Family and domain databases
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYTC2_DICDI

Accession

Primary (citable) accession number: Q8MP20
Secondary accession number(s): Q555M0, Q8T175

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)