Q8MNZ1 (GP63_LEITR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leishmanolysin
EC=3.4.24.36

Alternative name(s):
Cell surface protease
Major surface glycoprotein
Major surface protease
Promastigote surface endopeptidase
Protein gp63

Gene names

Name:

mspC

Organism

Leishmania tropica

Taxonomic identifier

5666 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Leishmania

Protein attributes

Sequence length	657 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has an integral role during the infection of macrophages in the mammalian host By similarity.
Catalytic activity	Preference for hydrophobic residues at P1 and P1' and basic residues at P2' and P3'. A model nonapeptide is cleaved at -Ala-Tyr-|-Leu-Lys-Lys-.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Sequence similarities	Belongs to the peptidase M8 family.

Ontologies

Keywords
Biological process	Cell adhesion
Coding sequence diversity	Polymorphism
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 41	41	Potential
	Propeptide	42 – 102	61	Activation peptide Potential		PRO_0000028672
	Chain	103 – 657	555	Leishmanolysin		PRO_0000028673
Sites
	Active site	267	1	By similarity
	Metal binding	266	1	Zinc; catalytic By similarity
	Metal binding	270	1	Zinc; catalytic By similarity
	Metal binding	336	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	107	1	N-linked (GlcNAc...) Potential
	Glycosylation	302	1	N-linked (GlcNAc...) Potential
	Glycosylation	399	1	N-linked (GlcNAc...) Potential
	Glycosylation	409	1	N-linked (GlcNAc...) Potential
	Glycosylation	445	1	N-linked (GlcNAc...) Potential
	Glycosylation	466	1	N-linked (GlcNAc...) Potential
	Glycosylation	501	1	N-linked (GlcNAc...) Potential
	Disulfide bond	127 ↔ 144		By similarity
	Disulfide bond	193 ↔ 232		By similarity
	Disulfide bond	316 ↔ 388		By similarity
	Disulfide bond	395 ↔ 458		By similarity
	Disulfide bond	408 ↔ 427		By similarity
	Disulfide bond	417 ↔ 492		By similarity
	Disulfide bond	469 ↔ 513		By similarity
	Disulfide bond	518 ↔ 568		By similarity
	Disulfide bond	538 ↔ 561		By similarity
Natural variations
	Natural variant	596 – 611	16	Missing in allele mspCLtA2.

Sequences

	Sequence		Length	Mass (Da)
	Q8MNZ1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 83FBC04757887E51	FASTA	657	70,343
	10 20 30 40 50 60 MSVDSSSSST HRRRCVAARL VRLAAAGAAV TVAVGTAAAW AHAGALQHRC IHDAMQARVR 70 80 90 100 110 120 QSVARHHTAP GAVSAVGLPY VTLDAAHTAA AADPRPGSAP TVVRAANWST LRVAVSTEDL 130 140 150 160 170 180 TDPAYHCARV GQRVNNHAGA IVTCTAEDIL TDEKRDILRK YLIPQALQLH TERLKARQVQ 190 200 210 220 230 240 GKWKVTGMVD EICGDFKVPQ AHITEGFSNT DFVMYVASVP SEEGVLAWAT TCQVFSDGHP 250 260 270 280 290 300 AVGVINIPAA NIASRYDQLV TRVVTHEMAH ALGFSEEFFT AARIVAHVSN VRHKTLKVPV 310 320 330 340 350 360 VNSSTAVAKA REQYGCGTLE YLEIEDQGGA GSAGSHIKMR NAQDELMAPA AAGGYYTALT 370 380 390 400 410 420 MAVFQDLGFY QADFNKAKVM PWGRNAGCAF LSEKCMEQNI TKWRAMFCNE SEDVMRCPTS 430 440 450 460 470 480 RLSLGTCGIR GYRPPLPRYW QYFTNASLGG YSPFMDYCPV VIGYANGSCN QDASSAAEFL 490 500 510 520 530 540 AAFNVFSEAA RCIDGAFTPK NRTAADGYYA GLCANVRCDT ATRTYSVQVR GSMDYVSCTP 550 560 570 580 590 600 GLRVELSTVS NAFEEGGCIT CPPYVEVCQG NVKGAKDFAG DSDSSSSADD AAGKAAMLRW 610 620 630 640 650 NDRMVGLATA ATVLLGMVLS LMALVVVWLL LVSCPWWCCK LGGPPASVTP ACSPETE

References

[1]	"Genetic diversity in the Leishmania donovani complex." Mauricio I.L., Stothard J.R., Miles M.A. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELES MSPCLTA1 AND MSPCLTA2). Strain: MHOM/SU/1974/K27.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ495008 Genomic DNA. Translation: CAD42817.1. AJ495009 Genomic DNA. Translation: CAD42818.1.
3D structure databases
ProteinModelPortal	Q8MNZ1.
SMR	Q8MNZ1. Positions 102-577.
ModBase	Search...
Protein family/group databases
MEROPS	M08.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001577. Peptidase_M8. [Graphical view]
PANTHER	PTHR10942. Peptidase_M8. 1 hit.
Pfam	PF01457. Peptidase_M8. 1 hit. [Graphical view]
PRINTS	PR00782. LSHMANOLYSIN.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GP63_LEITR

Accession

Primary (citable) accession number: Q8MNZ1
Secondary accession number(s): Q8MNZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	October 1, 2002
Last modified:	November 16, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)