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Protein

cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase B

Gene

pdeE

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dual specificity cAMP and cGMP phosphodiesterase with marked preference for cyclic AMP, which is activated by cAMP and cGMP. Likely functions as a cAMP-stimulated cAMP-phosphodiesterase which may play a role in regulating the cAMP relay response.3 Publications

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.
Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Cofactori

Mn2+1 Publication, Mg2+1 Publication, Zn2+1 PublicationNote: Divalent metal cation. Can use Mn2+ or, to a lower extent, Mg2+ or Zn2+. Half-maximal activation occurs between 10 and 100 uM of Mn2+ whereas maximal activation occurs with 10 mM of Zn2+ or Mg2+.1 Publication

Kineticsi

cAMP/cGMP selectivity of 9.
  1. KM=200 µM for cAMP2 Publications
  2. KM=800 µM for cGMP2 Publications
  1. Vmax=650 nmol/min/mg enzyme with cAMP as substrate2 Publications
  2. Vmax=300 nmol/min/mg enzyme with cGMP as substrate2 Publications

pH dependencei

Optimum pH is 7.0.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi573Divalent metal cationSequence analysis1
Metal bindingi575Divalent metal cationSequence analysis1
Metal bindingi577Divalent metal cationSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi783 – 930cNMP 1Add BLAST148
Nucleotide bindingi946 – 1070cNMP 2Add BLAST125

GO - Molecular functioni

  • 3',5'-cyclic-AMP phosphodiesterase activity Source: dictyBase
  • 3',5'-cyclic-GMP phosphodiesterase activity Source: dictyBase
  • cAMP binding Source: UniProtKB-KW
  • cGMP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cAMP catabolic process Source: dictyBase
  • cAMP-mediated signaling Source: dictyBase
  • cGMP-mediated signaling Source: dictyBase
  • protein localization to cell cortex Source: dictyBase
  • regulation of positive chemotaxis to cAMP Source: dictyBase

Keywordsi

Molecular functionHydrolase
LigandcAMP, cAMP-binding, cGMP, cGMP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

SABIO-RKiQ8MM62

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase B (EC:3.1.4.35, EC:3.1.4.53)
Alternative name(s):
Cyclic GMP-binding protein B
Phosphodiesterase 6
Short name:
DdPDE6
Phosphodiesterase E
Gene namesi
Name:pdeE
Synonyms:gbpB, pde6
ORF Names:DDB_G0276027
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0276027 pdeE

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: dictyBase
  • intracellular Source: dictyBase

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003531061 – 1096cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase BAdd BLAST1096

Proteomic databases

PaxDbiQ8MM62

Expressioni

Developmental stagei

Low expression during growth. Mainly expressed after 8-10 hours of starvation when cells are aggregating and in the multicellular stage.3 Publications

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0185220

Structurei

3D structure databases

ProteinModelPortaliQ8MM62
SMRiQ8MM62
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi214 – 218Poly-Ser5
Compositional biasi241 – 245Poly-Gln5
Compositional biasi457 – 460Poly-Ser4

Domaini

The beta lactamase-like domain catalyzes the hydrolysis of cGMP.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

InParanoidiQ8MM62
OMAiLSHINSC

Family and domain databases

CDDicd00038 CAP_ED, 2 hits
Gene3Di2.60.120.10, 2 hits
3.60.15.10, 1 hit
InterProiView protein in InterPro
IPR018490 cNMP-bd-like
IPR000595 cNMP-bd_dom
IPR001279 Metallo-B-lactamas
IPR036866 RibonucZ/Hydroxyglut_hydro
IPR014710 RmlC-like_jellyroll
PfamiView protein in Pfam
PF00027 cNMP_binding, 2 hits
PF00753 Lactamase_B, 1 hit
SMARTiView protein in SMART
SM00100 cNMP, 2 hits
SM00849 Lactamase_B, 1 hit
SUPFAMiSSF51206 SSF51206, 3 hits
SSF56281 SSF56281, 1 hit
PROSITEiView protein in PROSITE
PS50042 CNMP_BINDING_3, 2 hits

Sequencei

Sequence statusi: Complete.

Q8MM62-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSKYGDNII DFLRYLEKFV KSLTKDNKIE EFKTFDIKSL LYPRNKDYFG
60 70 80 90 100
NLSKFLLAVI SARIGSNFIN EDDIFEISEL LKEFLGQELE HLPYLSYEEL
110 120 130 140 150
YVEIVEQVGE INGTVSEIIE AITVTIDFLD TFEKVSQTID RSNEKQKLLA
160 170 180 190 200
YLSAPVNQLD NSVSGVFNEN DYTDIQRFFT ELTNENNQSP DSNISILIND
210 220 230 240 250
FQSLLNILES QQASSSSSKM IINDSPRTQQ RNGTTEQQKK QQQQQYLQKN
260 270 280 290 300
KEPFYSKDKI MQVSGPSYVF TPSDCNVSIQ VGIPPDTLKR DQSICHFIVP
310 320 330 340 350
HFLISKDVSL SEVEFPIFYN KFVQKGKTKV VIICTVEQKQ RIETILCESI
360 370 380 390 400
FGPAPEHIYT DEEITIPDYK IDLLTERLAI DPRANDEKLD SYVIFKTFDT
410 420 430 440 450
FGVVDIDLPS ASDPTKLINL RIRNTKGLIS FHEDYHVVQK QLHLKLQEQQ
460 470 480 490 500
QDQQDKSSSS TTDKQMINTS GNRIILKNNT VSVIDSTIES QYVPVLPFGN
510 520 530 540 550
DHEQVKKFKA PILGVTFLGV SHGLDFTHCS HTTGFIIWIN GSGVVVDPPV
560 570 580 590 600
GNTTYLQTNG IYGKTVEHII LTHCHADHDS GILQKIIERN KVTLYTTKTI
610 620 630 640 650
NESYMRKLKA LTGLPEQSLK NYYTWVPVTI GNKIKILGAE FEFDYSFHVI
660 670 680 690 700
PTIRFKLEIY NKKISYSADT FYDLQKFKQL KDQGVLSKKR IERLKSFVFD
710 720 730 740 750
ADMIIHESGV APIHTPMANL LELPSEIRKK IRVVHCSSSV DTKGEIIRPK
760 770 780 790 800
EGLENTEIIK VDRKYKGVAE CIQIQTALNH CSVFSKLSPA EVQRVFFLCK
810 820 830 840 850
KIWVKRNDVI IKKGSPSDMF YIILSGKVLV YENEYEPIKS TTSVGTVVTD
860 870 880 890 900
TTTITTTVKT DAIKIPTIKL CAGETLGESA LQLDKNIDAS ATVIAETDVC
910 920 930 940 950
LLVWKTMDLR TEFHSNLNTF ISKVHMDLSH INSCRDAIIR AFQHNITQHI
960 970 980 990 1000
NKEEVDSIAN GSKDVSFAHH QVIFNEGDTS DSMYIIKQGR VRIHSKKNKN
1010 1020 1030 1040 1050
IIRYLNVGDF FGETAYRRSN EDSNFLPTRS FTATAIDPTI LLKLDIESIV
1060 1070 1080 1090
NPRIQNIIEQ KAKKNAEDNI RYHAYSPKIR TPRTPRKVYP IEGLSI
Length:1,096
Mass (Da):124,924
Last modified:October 1, 2002 - v1
Checksum:i4F4F8CF199223782
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF481922 Genomic DNA Translation: AAM34040.1
AY047364 Genomic DNA Translation: AAL06060.1
AAFI02000014 Genomic DNA Translation: EAL69313.1
RefSeqiXP_643272.1, XM_638180.1

Genome annotation databases

EnsemblProtistsiEAL69313; EAL69313; DDB_G0276027
GeneIDi8620315
KEGGiddi:DDB_G0276027

Entry informationi

Entry nameiPDE6_DICDI
AccessioniPrimary (citable) accession number: Q8MM62
Secondary accession number(s): Q552E1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health