Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8MLZ3

- PDE5_DICDI

UniProt

Q8MLZ3 - PDE5_DICDI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

cGMP-dependent 3',5'-cGMP phosphodiesterase A

Gene

pdeD

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Phosphodiesterase specific for cGMP, which is activated by cGMP but not by cAMP. Involved in the degradation of intracellular cGMP.1 Publication

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Cofactori

Mn2+1 Publication, Mg2+1 Publication, Zn2+1 PublicationNote: Divalent metal cation. Can use Mn(2+) or, to a lower extent, Mg(2+) or Zn(2+). Half-maximal activation occurs between 10 and 100 uM of Mn(2+) whereas maximal activation occurs with 10 mM of Zn(2+) or Mg(2+).1 Publication

Kineticsi

cAMP/cGMP selectivity of 0.003.

  1. KM=5.2 µM for cGMP2 Publications

Vmax=390 pmol/min/mg enzyme2 Publications

pH dependencei

Optimum pH is 7.0.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi399 – 3991Divalent metal cationSequence Analysis
Metal bindingi401 – 4011Divalent metal cationSequence Analysis
Metal bindingi403 – 4031Divalent metal cationSequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi607 – 721115cNMP 1Add
BLAST
Nucleotide bindingi734 – 851118cNMP 2Add
BLAST

GO - Molecular functioni

  1. 3',5'-cyclic-GMP phosphodiesterase activity Source: dictyBase
  2. cGMP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cGMP catabolic process Source: dictyBase
  2. cGMP-mediated signaling Source: dictyBase
  3. signal transduction Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cGMP, cGMP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.4.35. 1939.

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-dependent 3',5'-cGMP phosphodiesterase A (EC:3.1.4.35)
Alternative name(s):
Cyclic GMP-binding protein A
Phosphodiesterase 5
Short name:
DdPDE5
Phosphodiesterase D
Gene namesi
Name:pdeD
Synonyms:gbpA, pde5
ORF Names:DDB_G0274383
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0274383. pdeD.

Subcellular locationi

Cytoplasmcytosol 3 Publications

GO - Cellular componenti

  1. cytosol Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 867867cGMP-dependent 3',5'-cGMP phosphodiesterase APRO_0000353105Add
BLAST

Expressioni

Developmental stagei

Expressed during growth and development, with the highest level of expression during aggregation.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0185054.

Structurei

3D structure databases

ProteinModelPortaliQ8MLZ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni357 – 503147Phosphodiesterase activityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi124 – 1274Poly-Ser
Compositional biasi142 – 1454Poly-Ser
Compositional biasi151 – 1544Poly-Gln
Compositional biasi155 – 1584Poly-Pro
Compositional biasi178 – 1858Poly-His
Compositional biasi189 – 1968Poly-Asn
Compositional biasi200 – 2034Poly-Asn
Compositional biasi209 – 22113Poly-GlnAdd
BLAST

Domaini

The beta lactamase-like domain catalyzes the hydrolysis of cGMP.

Sequence similaritiesi

Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG70621.
InParanoidiQ8MLZ3.
OMAiETIKDSM.
PhylomeDBiQ8MLZ3.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
3.60.15.10. 1 hit.
InterProiIPR001279. Beta-lactamas-like.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 2 hits.
SM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 2 hits.
SSF56281. SSF56281. 1 hit.
PROSITEiPS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8MLZ3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMFSKRKSIR FNPEGDYTEL PRGGYVVPSK LGGIQFGVPP ETIKDSMALK
60 70 80 90 100
IDVPTIYVFP EELWDRKTGI NAAEAEFPAY FNYFILKRKV SFVCTKEQEQ
110 120 130 140 150
RIRIVFQETL LGPPEFNTGI IINSSSSTTD TSKTSPIKKQ TSSSSPPLSP
160 170 180 190 200
QQQQPPPPLV KQPSQQQLEE LATMDTCHYH HHHHHQEVND NDNNNNTTTN
210 220 230 240 250
NNNIEILEQQ QQQQQQQQQQ QDEDSTDVDE EFQKEFSSTF PRSEIPNLEK
260 270 280 290 300
ECKYLRTFNS VDELFDFILF DDNGIAKLSD DVEIHFQEDQ SLFKVLQFEE
310 320 330 340 350
TGKGNKVQTL VATIPSKILF PDMINSISTI NDNKIFDPPT FGITIIGSSH
360 370 380 390 400
GFDPKKSTTG FVLWINKRGI MVDPPLNSSS FLQSQGVPTR MVDHIILTHC
410 420 430 440 450
HADHDSGTFQ KLLEEYQITV VTTPTILGSF LRKYGALSNL STDLLRRLFI
460 470 480 490 500
FRPVMIGEPM IISGAEFRFF YTIHTIPTIS FEVFYGGKSI FYSGDTCYDP
510 520 530 540 550
NRIKDMNKRG IMKTSRMKFF LRPWNHTVVL HEAGVPPIHT PVSVLRALPD
560 570 580 590 600
EVKNRLYLVH ISEHTLPAGS GLKIAKEGVA HTLSLDVMKS SHSEAVDILK
610 620 630 640 650
LVESVDIFRS IPLTQACEIL QTATKRKYSQ GSVIIARDTE PDAFYVVASG
660 670 680 690 700
VVCVNIGELK KNLIVGDYFG EMSLVMGGLR SANVQAVTDV EVLSFNKEDF
710 720 730 740 750
LSITRNSTES IQFITRLWEM RNEKSWETMS LNSVFSRCTN SQKTAIQSIL
760 770 780 790 800
VRELIKKDET LWCKGEEALF GCLVAEGSFV FKEDDSLESF SQGSFLGDIN
810 820 830 840 850
AMTTQPPSIH KTTVVAKEES VIYKVLSQDL IKFFSNNPGI QLAFLDTIFV
860
DALRDQVQQL VNSKLTY
Length:867
Mass (Da):97,992
Last modified:October 1, 2002 - v1
Checksum:iE203EED614E6F386
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF481921 Genomic DNA. Translation: AAM34039.1.
AY047363 Genomic DNA. Translation: AAL06059.1.
AAFI02000012 Genomic DNA. Translation: EAL70084.1.
RefSeqiXP_644314.1. XM_639222.1.

Genome annotation databases

EnsemblProtistsiDDB0185054; DDB0185054; DDB_G0274383.
GeneIDi8619742.
KEGGiddi:DDB_G0274383.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF481921 Genomic DNA. Translation: AAM34039.1 .
AY047363 Genomic DNA. Translation: AAL06059.1 .
AAFI02000012 Genomic DNA. Translation: EAL70084.1 .
RefSeqi XP_644314.1. XM_639222.1.

3D structure databases

ProteinModelPortali Q8MLZ3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0185054.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0185054 ; DDB0185054 ; DDB_G0274383 .
GeneIDi 8619742.
KEGGi ddi:DDB_G0274383.

Organism-specific databases

dictyBasei DDB_G0274383. pdeD.

Phylogenomic databases

eggNOGi NOG70621.
InParanoidi Q8MLZ3.
OMAi ETIKDSM.
PhylomeDBi Q8MLZ3.

Enzyme and pathway databases

BRENDAi 3.1.4.35. 1939.

Family and domain databases

Gene3Di 2.60.120.10. 2 hits.
3.60.15.10. 1 hit.
InterProi IPR001279. Beta-lactamas-like.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view ]
Pfami PF00027. cNMP_binding. 2 hits.
PF00753. Lactamase_B. 1 hit.
[Graphical view ]
SMARTi SM00100. cNMP. 2 hits.
SM00849. Lactamase_B. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 2 hits.
SSF56281. SSF56281. 1 hit.
PROSITEi PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium."
    Bosgraaf L., Russcher H., Smith J.L., Wessels D., Soll D.R., Van Haastert P.J.M.
    EMBO J. 21:4560-4570(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of a novel type of cGMP phosphodiesterase that is defective in the chemotactic stmF mutants."
    Meima M.E., Biondi R.M., Schaap P.
    Mol. Biol. Cell 13:3870-3877(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, COFACTOR, DEVELOPMENTAL STAGE.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  4. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  5. "Identification and characterization of two unusual cGMP-stimulated phoshodiesterases in dictyostelium."
    Bosgraaf L., Russcher H., Snippe H., Bader S., Wind J., Van Haastert P.J.M.
    Mol. Biol. Cell 13:3878-3889(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
  6. Cited for: IDENTIFICATION.
  7. "Characterization of a cAMP-stimulated cAMP phosphodiesterase in Dictyostelium discoideum."
    Meima M.E., Weening K.E., Schaap P.
    J. Biol. Chem. 278:14356-14362(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
  8. "Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP."
    Bader S., Kortholt A., Van Haastert P.J.M.
    Biochem. J. 402:153-161(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPDE5_DICDI
AccessioniPrimary (citable) accession number: Q8MLZ3
Secondary accession number(s): Q554K9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: October 1, 2002
Last modified: November 26, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3