Reviewed, UniProtKB/Swiss-Prot Q8MKI5 (CASP3_CANFA)

Names and origin

Protein names

Recommended name:
    Caspase-3
      Short name=CASP-3
    EC=3.4.22.56
Cleaved into the following 2 chains:
    1- Recommended name:
            Caspase-3 subunit p17
    2- Recommended name:
            Caspase-3 subunit p12

Gene names

Name:

CASP3

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 By similarity.
Catalytic activity	Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.
Subunit structure	Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit By similarity.
Subcellular location	Cytoplasm.
Post-translational modification	Cleavage by granzyme B, caspase-6, -8 and -10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa By similarity. S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol By similarity.
Sequence similarities	Belongs to the peptidase C14A family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein S-nitrosylation Zymogen
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 9	9	By similarity		PRO_0000004557
	Propeptide	10 – 28	19	By similarity		PRO_0000004558
	Chain	29 – 175	147	Caspase-3 subunit p17 By similarity		PRO_0000004559
	Chain	176 – 277	102	Caspase-3 subunit p12 By similarity		PRO_0000004560
Sites
	Active site	121	1	By similarity
	Active site	163	1	By similarity
Amino acid modifications
	Modified residue	26	1	Phosphoserine By similarity
	Modified residue	163	1	S-nitrosocysteine; in inhibited form By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8MKI5-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 7094C76D868BDAB9	FASTA	277	31,335
	10 20 30 40 50 60 MENTENSVDA KSFKNAETKI LHGSKSMDSG MSFDNSYKMD YPEMGLCIII NNKNFHKSTG 70 80 90 100 110 120 MAPRSGTDVD AANLRETFTN LKYEVRNKND LTCEEILELM NSVSKEDHSK RSSFVCVLLS 130 140 150 160 170 180 HGDEGIIFGT NGPVDLRKVT GFFRGDYCRS LTGKPKLFII QACRGTELDC GIETDSGIED 190 200 210 220 230 240 DMACQKIPVE ADFLYAYSTA PGYYSWRNSK DGSWFIQSLC AMLKLYAHKL EFMHILTRVN 250 260 270 RKVATEFESF SLDSAFHGKK QIPCIVSMLT KELYLYH

References

[1]	"Characterization of canine caspase-3." Sano J., Oguma K., Kano R., Hasegawa A. J. Vet. Med. Sci. 66:563-567(2004) [PubMed: 15187371] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB085580 mRNA. Translation: BAB92962.1.
RefSeq	NP_001003042.1.
UniGene	Cfa.84
3D structure databases
HSSP	HSSP built from PDB template 1PAU based on UniProtKB P42574.
ModBase	Search...
Protein family/group databases
MEROPS	C14.003.
Genome annotation databases
Ensembl	ENSCAFG00000007750. Canis familiaris. [Contig view]
GeneID	403567.
KEGG	cfa:403567.
Phylogenomic databases
HOVERGEN	Q8MKI5.
Enzyme and pathway databases
BRENDA	3.4.22.56. 463.
Family and domain databases
InterPro	IPR015470. Caspase_3_related. IPR011600. Pept_C14_cat. IPR001309. Pept_C14_ICE_p20. IPR016129. Pept_C14_ICE_p20_AS. IPR002138. Pept_C14_p10. IPR002398. Pept_C14_p45. IPR015917. Pept_C14_p45_core. [Graphical view]
PANTHER	PTHR10454:SF30. Casp3_like. 1 hit. PTHR10454. Pept_C14_p45. 1 hit.
Pfam	PF00656. Peptidase_C14. 1 hit. [Graphical view]
PRINTS	PR00376. IL1BCENZYME.
SMART	SM00115. CASc. 1 hit. [Graphical view]
PROSITE	PS01122. CASPASE_CYS. 1 hit. PS01121. CASPASE_HIS. 1 hit. PS50207. CASPASE_P10. 1 hit. PS50208. CASPASE_P20. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CASP3_CANFA

Accession

Primary (citable) accession number: Q8MKI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)