Reviewed, UniProtKB/Swiss-Prot Q8MKF1 (THTPA_BOVIN)

Names and origin

Protein names

Recommended name:
    Thiamine-triphosphatase
      Short name=ThTPase
    EC=3.6.1.28

Gene names

Name:

THTPA

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolase highly specific for thiamine triphosphate (ThTP). Ref.1
Catalytic activity	Thiamine triphosphate + H2O = thiamine diphosphate + phosphate.
Subunit structure	Monomer. Ref.1
Subcellular location	Cytoplasm. Ref.1
Sequence similarities	Belongs to the ThTPase family.
Mass spectrometry	Molecular mass is 23892 Da from positions 2 - 219. Determined by ESI. Ref.1

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cAMP biosynthetic process Inferred from electronic annotation. Source: InterPro thiamin metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenylate cyclase activity Inferred from electronic annotation. Source: InterPro thiamin-triphosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 219	218	Thiamine-triphosphatase		PRO_0000221489
Amino acid modifications
	Modified residue	2	1	N-acetylalanine

Sequences

	Sequence		Length	Mass (Da)
	Q8MKF1-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 6E617AA2FDB7ACC3	FASTA	219	23,983
	10 20 30 40 50 60 MAQGLIEVER KFVPGPSTEE RLQELGGTLE HRVTFRDSYY DTPELSLMRA DYWLRQREGS 70 80 90 100 110 120 GWELKCPGAA GVSGPHTEYT ELTAEPSIVA QLCEVLGAEV PGAGGVAAVL GPLGLQLVAS 130 140 150 160 170 180 FVTKRSAWKL VLSGADGEER LLRVDLDTAD FGYAVGEVEA LVHKEAEVPA ALEKIHHLSS 190 200 210 LLGVLEQGRA PAKLIVYLQR FRPQDYQRLL EVYGSKEKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of a specific thiamine triphosphatase widely expressed in mammalian tissues." Lakaye B., Makarchikov A.F., Antunes A.F., Zorzi W., Coumans B., De Pauw E., Wins P., Grisar T., Bettendorff L. J. Biol. Chem. 277:13771-13777(2002) [PubMed: 11827967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, CHARACTERIZATION, MASS SPECTROMETRY.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF432863 mRNA. Translation: AAM22404.1. BC105468 mRNA. Translation: AAI05469.1.
IPI	IPI00714789.
RefSeq	NP_776895.1.
UniGene	Bt.8966
3D structure databases
SMR	Q8MKF1. Positions 2-217.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000007097. Bos taurus. [Contig view]
GeneID	282090.
KEGG	bta:282090.
Phylogenomic databases
HOVERGEN	Q8MKF1.
OMA	Q8MKF1. EVERKFV.
Enzyme and pathway databases
BRENDA	3.6.1.28. 251.
Family and domain databases
InterPro	IPR008172. Adenylate_cyclase. IPR012177. ThTPase. [Graphical view]
PANTHER	PTHR14586. ThTPase. 1 hit.
Pfam	PF01928. CYTH. 1 hit. [Graphical view]
PIRSF	PIRSF036561. ThTPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THTPA_BOVIN

Accession

Primary (citable) accession number: Q8MKF1
Secondary accession number(s): Q2KJ88

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2003
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 44 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)