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Protein

Diacylglycerol O-acyltransferase 1

Gene

DGAT1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders. In liver, plays a role in esterifying exogenous fatty acids to glycerol.By similarity

Catalytic activityi

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.By similarity
Acyl-CoA + retinol = CoA + retinyl ester.By similarity

Pathwayi: glycerolipid metabolism

This protein is involved in the pathway glycerolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway glycerolipid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei416 – 4161By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.20. 908.
UniPathwayiUPA00230.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol O-acyltransferase 1 (EC:2.3.1.20)
Alternative name(s):
Acyl-CoA retinol O-fatty-acyltransferase (EC:2.3.1.76)
Short name:
ARAT
Short name:
Retinol O-fatty-acyltransferase
Diglyceride acyltransferase
Gene namesi
Name:DGAT1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8484CytoplasmicSequence analysisAdd
BLAST
Transmembranei85 – 10622HelicalSequence analysisAdd
BLAST
Topological domaini107 – 218112LumenalSequence analysisAdd
BLAST
Transmembranei219 – 23012HelicalSequence analysisAdd
BLAST
Topological domaini231 – 2311CytoplasmicSequence analysis
Transmembranei232 – 2409HelicalSequence analysis
Topological domaini241 – 489249LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 489489Diacylglycerol O-acyltransferase 1PRO_0000244770Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei20 – 201PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8MK44.
PRIDEiQ8MK44.

Interactioni

Subunit structurei

Homodimer or homotetramer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000037256.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 8888Involved in homomerizationBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0380. Eukaryota.
COG5056. LUCA.
HOVERGENiHBG051341.
InParanoidiQ8MK44.
KOiK11155.

Family and domain databases

InterProiIPR027251. Diacylglycerol_acylTrfase1.
IPR004299. MBOAT_fam.
IPR014371. Oat_ACAT_DAG_ARE.
[Graphical view]
PANTHERiPTHR10408. PTHR10408. 1 hit.
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000439. Oat_ACAT_DAG_ARE. 1 hit.
PIRSF500231. Oat_dag. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8MK44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDRGGAGGS RRRRTGSRPS IQGGSGPAAA EEEVRDVGAG GDAPVRDTDK
60 70 80 90 100
DGDVDVGSGH WDLRCHRLQD SLFSSDSGFS NYRGILNWCV VMLILSNARL
110 120 130 140 150
FLENLIKYGI LVDPIQVVSL FLKDPYSWPA LCLVIVANIF AVAAFQVEKR
160 170 180 190 200
LAVGALTEQA GLLLHGVNLA TILCFPAAVA FLLESITPVG SVLALMVYTI
210 220 230 240 250
LFLKLFSYRD VNLWCRERRA GAKAKAALAG KKANGGAAQR TVSYPDNLTY
260 270 280 290 300
RDLYYFLFAP TLCYELNFPR SPRIRKRFLL RRLLEMLFLT QLQVGLIQQW
310 320 330 340 350
MVPAIQNSMK PFKDMDYSRI VERLLKLAVP NHLIWLIFFY WLFHSCLNAV
360 370 380 390 400
AELMQFGDRE FYRDWWNSES ITYFWQNWNI PVHKWCIRHF YKPMLRRGSS
410 420 430 440 450
KWAARTAVFL ASAFFHEYLV SIPLRMFRLW AFTGMMAQIP LAWIVGRFFR
460 470 480
GNYGNAAVWL SLIIGQPVAV LMYVHDYYVL NREAPAAGT
Length:489
Mass (Da):55,602
Last modified:June 27, 2006 - v2
Checksum:i4066D8C1B6743253
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti425 – 4251R → G in CAC86391 (PubMed:12077321).Curated

Polymorphismi

Lys-232 is associated with higher milk fat content.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti232 – 2321K → A.3 Publications

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY065621 Genomic DNA. Translation: AAL49962.1.
AJ318490 Genomic DNA. Translation: CAC86391.1.
BC118146 mRNA. Translation: AAI18147.1.
RefSeqiNP_777118.2. NM_174693.2.
UniGeneiBt.61989.

Genome annotation databases

GeneIDi282609.
KEGGibta:282609.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY065621 Genomic DNA. Translation: AAL49962.1.
AJ318490 Genomic DNA. Translation: CAC86391.1.
BC118146 mRNA. Translation: AAI18147.1.
RefSeqiNP_777118.2. NM_174693.2.
UniGeneiBt.61989.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000037256.

Proteomic databases

PaxDbiQ8MK44.
PRIDEiQ8MK44.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi282609.
KEGGibta:282609.

Organism-specific databases

CTDi8694.

Phylogenomic databases

eggNOGiKOG0380. Eukaryota.
COG5056. LUCA.
HOVERGENiHBG051341.
InParanoidiQ8MK44.
KOiK11155.

Enzyme and pathway databases

UniPathwayiUPA00230.
BRENDAi2.3.1.20. 908.

Miscellaneous databases

NextBioi20806313.

Family and domain databases

InterProiIPR027251. Diacylglycerol_acylTrfase1.
IPR004299. MBOAT_fam.
IPR014371. Oat_ACAT_DAG_ARE.
[Graphical view]
PANTHERiPTHR10408. PTHR10408. 1 hit.
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000439. Oat_ACAT_DAG_ARE. 1 hit.
PIRSF500231. Oat_dag. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition."
    Grisart B., Coppieters W., Farnir F., Karim L., Ford C., Berzi P., Cambisano N., Mni M., Reid S., Simon P., Spelman R., Georges M., Snell R.
    Genome Res. 12:222-231(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-232.
  2. "Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA:diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content."
    Winter A., Kramer W., Werner F.A.O., Kollers S., Kata S., Durstewitz G., Buitkamp J., Womack J.E., Thaller G., Fries R.
    Proc. Natl. Acad. Sci. U.S.A. 99:9300-9305(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-232.
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-232.
    Strain: Hereford.
    Tissue: Ascending colon.

Entry informationi

Entry nameiDGAT1_BOVIN
AccessioniPrimary (citable) accession number: Q8MK44
Secondary accession number(s): Q148M3, Q8SQB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: April 13, 2016
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.