Reviewed, UniProtKB/Swiss-Prot Q8MK44 (DGAT1_BOVIN)

Names and origin

Protein names

Recommended name:
    Diacylglycerol O-acyltransferase 1
    EC=2.3.1.20
Alternative name(s):
    Diglyceride acyltransferase

Gene names

Name:

DGAT1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly By similarity.
Catalytic activity	Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
Pathway	Lipid metabolism; glycerolipid metabolism.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Polymorphism	Lys-232 is associated with higher milk fat content.
Sequence similarities	Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	diacylglycerol O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 489	489	Diacylglycerol O-acyltransferase 1		PRO_0000244770
Regions
	Transmembrane	125 – 145	21	Potential
	Transmembrane	162 – 182	21	Potential
	Transmembrane	186 – 206	21	Potential
	Transmembrane	283 – 303	21	Potential
	Transmembrane	333 – 353	21	Potential
	Transmembrane	407 – 427	21	Potential
	Transmembrane	429 – 449	21	Potential
	Transmembrane	454 – 474	21	Potential
Amino acid modifications
	Modified residue	20	1	Phosphoserine By similarity
Natural variations
	Natural variant	232	1	K → A
Experimental info
	Sequence conflict	425	1	R → G in CAC86391. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8MK44-1 [UniParc]. Last modified June 27, 2006. Version 2. Checksum: 4066D8C1B6743253	FASTA	489	55,602
	10 20 30 40 50 60 MGDRGGAGGS RRRRTGSRPS IQGGSGPAAA EEEVRDVGAG GDAPVRDTDK DGDVDVGSGH 70 80 90 100 110 120 WDLRCHRLQD SLFSSDSGFS NYRGILNWCV VMLILSNARL FLENLIKYGI LVDPIQVVSL 130 140 150 160 170 180 FLKDPYSWPA LCLVIVANIF AVAAFQVEKR LAVGALTEQA GLLLHGVNLA TILCFPAAVA 190 200 210 220 230 240 FLLESITPVG SVLALMVYTI LFLKLFSYRD VNLWCRERRA GAKAKAALAG KKANGGAAQR 250 260 270 280 290 300 TVSYPDNLTY RDLYYFLFAP TLCYELNFPR SPRIRKRFLL RRLLEMLFLT QLQVGLIQQW 310 320 330 340 350 360 MVPAIQNSMK PFKDMDYSRI VERLLKLAVP NHLIWLIFFY WLFHSCLNAV AELMQFGDRE 370 380 390 400 410 420 FYRDWWNSES ITYFWQNWNI PVHKWCIRHF YKPMLRRGSS KWAARTAVFL ASAFFHEYLV 430 440 450 460 470 480 SIPLRMFRLW AFTGMMAQIP LAWIVGRFFR GNYGNAAVWL SLIIGQPVAV LMYVHDYYVL NREAPAAGT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition." Grisart B., Coppieters W., Farnir F., Karim L., Ford C., Berzi P., Cambisano N., Mni M., Reid S., Simon P., Spelman R., Georges M., Snell R. Genome Res. 12:222-231(2002) [PubMed: 11827942] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-232.
[2]	"Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA:diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content." Winter A., Kramer W., Werner F.A.O., Kollers S., Kata S., Durstewitz G., Buitkamp J., Womack J.E., Thaller G., Fries R. Proc. Natl. Acad. Sci. U.S.A. 99:9300-9305(2002) [PubMed: 12077321] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-232.
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-232. Strain: Hereford. Tissue: Ascending colon.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY065621 Genomic DNA. Translation: AAL49962.1. AJ318490 Genomic DNA. Translation: CAC86391.1. BC118146 mRNA. Translation: AAI18147.1.
IPI	IPI00710817.
RefSeq	NP_777118.2.
UniGene	Bt.61989
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8MK44.
Genome annotation databases
GeneID	282609.
KEGG	bta:282609.
Organism-specific databases
CTD	282609.
Phylogenomic databases
HOVERGEN	Q8MK44.
Enzyme and pathway databases
BRENDA	2.3.1.20. 251.
Family and domain databases
InterPro	IPR004299. MBOAT_fam. [Graphical view]
Pfam	PF03062. MBOAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DGAT1_BOVIN

Accession

Primary (citable) accession number: Q8MK44
Secondary accession number(s): Q148M3, Q8SQB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	June 27, 2006
Last modified:	October 13, 2009
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)