Reviewed, UniProtKB/Swiss-Prot Q8MIR0 (TDH_PIG)

Names and origin

Protein names

Recommended name:
    L-threonine 3-dehydrogenase, mitochondrial
    EC=1.1.1.103

Gene names

Name:

TDH

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH. Ref.2
Pathway	Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 1/2.
Subcellular location	Mitochondrion Ref.2.
Sequence similarities	Belongs to the sugar epimerase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cellular metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-threonine 3-dehydrogenase activity Inferred from electronic annotation. Source: EC coenzyme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion
	Chain	? – 373		L-threonine 3-dehydrogenase, mitochondrial		PRO_0000298785
Regions
	Nucleotide binding	9 – 46	38	NAD By similarity UniProtKB Q8R059
Sites
	Active site	195	1	Proton acceptor By similarity UniProtKB Q8R059
Experimental info
	Sequence conflict	65	1	G → Q AA sequence Ref.2
	Sequence conflict	309	1	K → R AA sequence Ref.2
	Sequence conflict	331	1	W → I AA sequence Ref.2
	Sequence conflict	341	1	R → A AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8MIR0-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 6F3CCB6879E66480	FASTA	373	41,432
	10 20 30 40 50 60 MPVVKMLKQV ASRTLGSPAC GCQPPTLPRR FLGTSPRQIP ADANFHSTSF SEANQPRVLI 70 80 90 100 110 120 TGGLGQLGVG LASLLRKRFG KDNVILSDIR KPPEHVFLSG PFIYSDILDY KNLREIVVNN 130 140 150 160 170 180 RVTWLFHYSA LLSAVGEANV SLARAVNITG LHNVLDVAAE HGLRLFVPST IGAFGPTSPR 190 200 210 220 230 240 NPTPDLCIQR PRTIYGVSKV HAELMGEYYY YRYGLDFRCL RYPGIISADS QPGGGTTDYA 250 260 270 280 290 300 VQIFQDAVKN GRFECNLNPG TKLPMMYIDD CLRATLEVME APAEALSLRT YNVNAMSFTP 310 320 330 340 350 360 AELAQEVLKH IPEFQITYNV DSVRQAIADS WPMNFDDSTA RRDWGWKHDF DLPELVTTML 370 NFHGAHSRVA QAN

References

[1]	"Molecular cloning and tissue distribution of mammalian L-threonine 3-dehydrogenases." Edgar A.J. BMC Biochem. 3:19-19(2002) [PubMed: 12097150] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Purification and structural characterization of porcine L-threonine dehydrogenase." Kao Y.-C., Davis L. Protein Expr. Purif. 5:423-431(1994) [PubMed: 7827500] [Abstract] Cited for: PROTEIN SEQUENCE OF 51-111; 145-180; 200-218; 222-249 AND 274-342, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY095535 mRNA. Translation: AAM18208.1.
RefSeq	NP_999169.1.
UniGene	Ssc.51
3D structure databases
SMR	Q8MIR0. Positions 55-362.
ModBase	Search...
Genome annotation databases
GeneID	397065.
KEGG	ssc:397065.
Organism-specific databases
CTD	397065.
Phylogenomic databases
HOVERGEN	Q8MIR0.
Enzyme and pathway databases
BRENDA	1.1.1.103. 249.
Family and domain databases
InterPro	IPR001509. Epimerase_deHydtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01370. Epimerase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TDH_PIG

Accession

Primary (citable) accession number: Q8MIR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	October 1, 2002
Last modified:	January 19, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)