Q8MIF7 (PGK2_HORSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycerate kinase 2
EC=2.7.2.3

Alternative name(s):
Phosphoglycerate kinase, testis specific

Gene names

Name:

PGK2

Organism

Equus caballus (Horse) [Complete proteome]

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from sequence or structural similarity. Source: UniProtKB phosphoglycerate kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 417	416	Phosphoglycerate kinase 2		PRO_0000145830
Regions
	Nucleotide binding	373 – 376	4	ATP By similarity
	Region	24 – 26	3	Substrate binding By similarity
	Region	63 – 66	4	Substrate binding By similarity
Sites
	Binding site	39	1	Substrate By similarity
	Binding site	123	1	Substrate By similarity
	Binding site	171	1	Substrate By similarity
	Binding site	220	1	ATP By similarity
	Binding site	313	1	ATP; via carbonyl oxygen By similarity
	Binding site	344	1	ATP By similarity
Amino acid modifications
	Modified residue	91	1	N6-acetyllysine By similarity
	Modified residue	156	1	N6-acetyllysine By similarity
	Modified residue	267	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8MIF7 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 5AA58A6CF3685392	FASTA	417	44,879
	10 20 30 40 50 60 MSLSKKLTLD KLDVKGKRII MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGARSVVL 70 80 90 100 110 120 MSHLGRPDGV PMPDKYSLEP VAAELKSLLG KDVLFLKDCV GSEVEKACAN PATGSVILLE 130 140 150 160 170 180 NLRFHVEEEG KGQDPSGNKL KAEAGKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGIN 190 200 210 220 230 240 LPQKASGFLM KKELEYFAKA LENPERPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM 250 260 270 280 290 300 AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKANKNGVRI TFPVDFVTAD KFEENAKVGQ 310 320 330 340 350 360 ATVASGIPAG WMGLDCGPET NKKYAQVMAQ AKLIVWNGPV GVFEWDAFAK GTKALMDEIV 370 380 390 400 410 KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VDALSNL

References

[1]	"Molecular characterization of the equine AEG1 locus." Giese A., Jude R., Kuiper H., Piumi F., Schambony A., Guerin G., Distl O., Topfer-Petersen E., Leeb T. Gene 292:65-72(2002) [PubMed: 12119100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ315378 Genomic DNA. Translation: CAD43034.1.
RefSeq	NP_001077063.1. NM_001083594.1.
UniGene	Eca.13389.
3D structure databases
ProteinModelPortal	Q8MIF7.
SMR	Q8MIF7. Positions 2-417.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100036552.
KEGG	ecb:100036552.
Organism-specific databases
CTD	5232.
Phylogenomic databases
HOVERGEN	HBG008177.
Family and domain databases
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
KO	K00927.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PGK2_HORSE

Accession

Primary (citable) accession number: Q8MIF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 49 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program